Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P97436

- NKX31_MOUSE

UniProt

P97436 - NKX31_MOUSE

Protein

Homeobox protein Nkx-3.1

Gene

Nkx3-1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor By similarity. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to suppress growth and tumorigenicity of prostate carcinoma cells. Plays a role in the formation of minor salivary glands (particularly palatine and lingual glands). Essential for appropriate differentiation and secretory function of the bulbourethral gland.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi125 – 18460HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. androgen receptor activity Source: UniProtKB
    2. core promoter binding Source: UniProtKB
    3. cysteine-type endopeptidase activator activity involved in apoptotic process Source: Ensembl
    4. DNA binding Source: MGI
    5. estrogen receptor activity Source: UniProtKB
    6. estrogen receptor binding Source: UniProtKB
    7. MADS box domain binding Source: UniProtKB
    8. protein binding Source: MGI
    9. protein kinase activator activity Source: UniProtKB
    10. protein self-association Source: UniProtKB
    11. sequence-specific DNA binding Source: UniProtKB
    12. sequence-specific DNA binding transcription factor activity Source: MGI
    13. transcription factor binding Source: UniProtKB
    14. transcription regulatory region sequence-specific DNA binding Source: UniProtKB

    GO - Biological processi

    1. activation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    2. androgen receptor signaling pathway Source: UniProtKB
    3. branching involved in prostate gland morphogenesis Source: MGI
    4. branching morphogenesis of an epithelial tube Source: MGI
    5. cellular response to drug Source: Ensembl
    6. cellular response to hypoxia Source: Ensembl
    7. cellular response to interleukin-1 Source: Ensembl
    8. cellular response to steroid hormone stimulus Source: UniProtKB
    9. cellular response to tumor necrosis factor Source: Ensembl
    10. dorsal aorta development Source: UniProtKB
    11. epithelial cell proliferation involved in salivary gland morphogenesis Source: MGI
    12. heart development Source: BHF-UCL
    13. internal genitalia morphogenesis Source: BHF-UCL
    14. male gonad development Source: UniProtKB
    15. metanephros development Source: UniProtKB
    16. mitotic cell cycle arrest Source: Ensembl
    17. negative regulation of cell proliferation Source: MGI
    18. negative regulation of epithelial cell proliferation Source: MGI
    19. negative regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
    20. negative regulation of estrogen receptor binding Source: UniProtKB
    21. negative regulation of insulin-like growth factor receptor signaling pathway Source: MGI
    22. negative regulation of mitotic cell cycle Source: Ensembl
    23. negative regulation of transcription, DNA-templated Source: UniProtKB
    24. pharyngeal system development Source: BHF-UCL
    25. positive regulation of androgen secretion Source: UniProtKB
    26. positive regulation of cell death Source: UniProtKB
    27. positive regulation of cell division Source: UniProtKB
    28. positive regulation of cell proliferation Source: UniProtKB
    29. positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: UniProtKB
    30. positive regulation of gene expression Source: UniProtKB
    31. positive regulation of intrinsic apoptotic signaling pathway Source: UniProtKB
    32. positive regulation of mitotic cell cycle Source: UniProtKB
    33. positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
    34. positive regulation of protein phosphorylation Source: UniProtKB
    35. positive regulation of response to DNA damage stimulus Source: UniProtKB
    36. positive regulation of transcription, DNA-templated Source: MGI
    37. positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    38. prostate gland development Source: UniProtKB
    39. protein kinase B signaling Source: UniProtKB
    40. response to testosterone Source: UniProtKB
    41. salivary gland development Source: MGI
    42. somitogenesis Source: BHF-UCL
    43. steroid hormone mediated signaling pathway Source: GOC
    44. transcription from RNA polymerase II promoter Source: GOC
    45. urogenital system development Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein Nkx-3.1
    Alternative name(s):
    Homeobox protein NK-3 homolog A
    Gene namesi
    Name:Nkx3-1
    Synonyms:Nkx-3.1, Nkx3a
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:97352. Nkx3-1.

    Subcellular locationi

    Nucleus PROSITE-ProRule annotation

    GO - Cellular componenti

    1. intracellular Source: UniProtKB
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Keywords - Diseasei

    Tumor suppressor

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 237237Homeobox protein Nkx-3.1PRO_0000048946Add
    BLAST

    Post-translational modificationi

    Ubiquitinated by TOPORS; monoubiquitinated at several residues and also polyubiquitinated on single residues.By similarity

    Keywords - PTMi

    Ubl conjugation

    Proteomic databases

    PRIDEiP97436.

    PTM databases

    PhosphoSiteiP97436.

    Expressioni

    Tissue specificityi

    Expressed mostly in the male urogenital tract, with highest expression in the epithelial cells lining the ducts of anterior, dorsolateral and ventral prostate and in the bulbourethral gland, and much lower in the seminal vesicle and the testis. Expression in the prostate increases during sexual maturation and is drastically reduced following castration. Expressed also in brain (hippocampus and external granular layer of the cerebral cortex), kidney (intralobular arteries), thymus and adrenal and salivary glands.

    Developmental stagei

    Early marker of the sclerotome and of a subset of vascular smooth muscle cells, expressed also in outgrowths of epithelial cells, in ectodermal epithelial cells and in restricted regions of the central nervous system. Detected first at 7.5 dpc in the paraxial mesoderm adjacent to the neural fold. At 8.5 dpc, segmental expression in the first 8 or 9 somites. Expression proceeds caudally in parallel with somite maturation and is restricted to the sclerotome. As the somites mature, expression moves away from the axial structures, becomes transiently restricted to a subset of early myotomal cells at the dorsal medial lip and is subsequently down-regulated. At 10.5 dpc, expressed only in the most caudal immature somites. At 9.5 dpc, present in the dorsal aorta. At 11.5 dpc, restricted to the vascular smooth muscle cells of caudal region of the dorsal aorta. At 12.5 dpc, expressed in the distal epithelium of the tongue and in Rathke pouch (anterior pituitary). By 13.5 dpc, also detected in tooth buds. Expression in the abdominal aorta continues through 11.5 to 15.5 dpc. Detected in the vertebral vessels at 12.5 dpc, in the carotid vessel at 13.5 dpc and in arcuate and interlobular arteries of the kidney at 15.5 dpc. In neonates, present in palatine glands, epithelial root sheath of the tooth and epithelial hair sheath. In the nervous system of neonates, expressed in the olfactory lobe, olfactory epithelial cells and cerebellar cortex. Expressed in the male urogenital system during late embryogenesis: at day 14.5, expressed in the outbuddings of the pelvic region of the urogenital sinus, and, at lower levels, in the prospective urethra. Expression is confined to the epithelial cells that are invaginating into the surrounding mesenchyme, with highest levels at the leading edge. At 17.5 dpc, present in the developing ventral, dorsolateral and anterior prostatic buds, in the nascent bulbourethral glands, as well as in the epithelial ducts that join the glands to the prospective urethra. During postnatal growth and morphogenesis of the prostate, high expression is maintain at sites of ductal outgrowth and branching. In the developing testis, detected at 14.5 and 17.5 dpc in the medullary cords, which form seminiferous tubules.

    Inductioni

    By androgens. During embryonic development, induced and maintained by sonic hedgehog in pre-somitic mesoderm, in immature somites and in urogenital sinus, but not in the other expression domains.

    Gene expression databases

    ArrayExpressiP97436.
    BgeeiP97436.
    CleanExiMM_NKX3-1.
    GenevestigatoriP97436.

    Interactioni

    Subunit structurei

    Interacts with serum response factor (SRF). Interacts with SPDEF By similarity. Interacts with WDR77 By similarity. Interacts with TOPORS which polyubiquitinates NKX3-1 and induces its proteasomal degradation By similarity.By similarity

    Protein-protein interaction databases

    BioGridi201781. 2 interactions.
    STRINGi10090.ENSMUSP00000022646.

    Structurei

    3D structure databases

    ProteinModelPortaliP97436.
    SMRiP97436. Positions 135-190.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NK-3 homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG238786.
    HOGENOMiHOG000231923.
    HOVERGENiHBG006689.
    InParanoidiP97436.
    KOiK09348.
    OMAiGAQRQGG.
    OrthoDBiEOG7QZG9Z.
    PhylomeDBiP97436.
    TreeFamiTF315720.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    [Graphical view]
    PRINTSiPR00024. HOMEOBOX.
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97436-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLRVAEPREP RVEAGGRSPW AAPPTQSKRL TSFLIQDILR DRAERHGGHS    50
    GNPQHSPDPR RDSAPEPDKA GGRGVAPEDP PSIRHSPAET PTEPESDAHF 100
    ETYLLDCEHN PGDLASAPQV TKQPQKRSRA AFSHTQVIEL ERKFSHQKYL 150
    SAPERAHLAK NLKLTETQVK IWFQNRRYKT KRKQLSEDLG VLEKNSPLSL 200
    PALKDDSLPS TSLVSVYTSY PYYPYLYCLG SWHPSFW 237
    Length:237
    Mass (Da):26,824
    Last modified:May 1, 1997 - v1
    Checksum:i4B074387F3BA1223
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73460 mRNA. Translation: AAC52956.1.
    U88542 mRNA. Translation: AAB58025.1.
    CCDSiCCDS27237.1.
    RefSeqiNP_035051.1. NM_010921.3.
    UniGeneiMm.3520.

    Genome annotation databases

    EnsembliENSMUST00000022646; ENSMUSP00000022646; ENSMUSG00000022061.
    GeneIDi18095.
    KEGGimmu:18095.
    UCSCiuc007umd.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73460 mRNA. Translation: AAC52956.1 .
    U88542 mRNA. Translation: AAB58025.1 .
    CCDSi CCDS27237.1.
    RefSeqi NP_035051.1. NM_010921.3.
    UniGenei Mm.3520.

    3D structure databases

    ProteinModelPortali P97436.
    SMRi P97436. Positions 135-190.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201781. 2 interactions.
    STRINGi 10090.ENSMUSP00000022646.

    PTM databases

    PhosphoSitei P97436.

    Proteomic databases

    PRIDEi P97436.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022646 ; ENSMUSP00000022646 ; ENSMUSG00000022061 .
    GeneIDi 18095.
    KEGGi mmu:18095.
    UCSCi uc007umd.1. mouse.

    Organism-specific databases

    CTDi 4824.
    MGIi MGI:97352. Nkx3-1.

    Phylogenomic databases

    eggNOGi NOG238786.
    HOGENOMi HOG000231923.
    HOVERGENi HBG006689.
    InParanoidi P97436.
    KOi K09348.
    OMAi GAQRQGG.
    OrthoDBi EOG7QZG9Z.
    PhylomeDBi P97436.
    TreeFami TF315720.

    Miscellaneous databases

    NextBioi 293247.
    PROi P97436.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97436.
    Bgeei P97436.
    CleanExi MM_NKX3-1.
    Genevestigatori P97436.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR020479. Homeobox_metazoa.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    [Graphical view ]
    PRINTSi PR00024. HOMEOBOX.
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Prostate-specific and androgen-dependent expression of a novel homeobox gene."
      Bieberich C.J., Fujita K., He W.-W., Jay G.
      J. Biol. Chem. 271:31779-31782(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Prostate.
    2. "Tissue-specific expression of murine Nkx3.1 in the male urogenital system."
      Sciavolino P.J., Abrams E.W., Yang L., Austenberg L.P., Shen M.M., Abate-Shen C.
      Dev. Dyn. 209:127-138(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss Webster.
      Tissue: Embryo.
    3. "Nkx3.1 mutant mice recapitulate early stages of prostate carcinogenesis."
      Kim M.J., Bhatia-Gaur R., Banach-Petrosky W.A., Desai N., Wang Y., Hayward S.W., Cunha G.R., Cardiff R.D., Shen M.M., Abate-Shen C.
      Cancer Res. 62:2999-3004(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION AS TUMOR SUPPRESSOR.

    Entry informationi

    Entry nameiNKX31_MOUSE
    AccessioniPrimary (citable) accession number: P97436
    Secondary accession number(s): O09087
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3