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P97435

- ENTK_MOUSE

UniProt

P97435 - ENTK_MOUSE

Protein

Enteropeptidase

Gene

Tmprss15

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases By similarity.By similarity

    Catalytic activityi

    Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei874 – 8741Charge relay systemBy similarity
    Active sitei925 – 9251Charge relay systemBy similarity
    Active sitei1021 – 10211Charge relay systemBy similarity

    GO - Molecular functioni

    1. scavenger receptor activity Source: InterPro
    2. serine-type endopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.156.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Enteropeptidase (EC:3.4.21.9)
    Alternative name(s):
    Enterokinase
    Serine protease 7
    Transmembrane protease serine 15
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Tmprss15
    Synonyms:Entk, Prss7
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 16

    Organism-specific databases

    MGIiMGI:1197523. Tmprss15.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 829829Enteropeptidase non-catalytic heavy chainPRO_0000027721Add
    BLAST
    Chaini830 – 1069240Enteropeptidase catalytic light chainPRO_0000027722Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi147 – 1471N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi229 ↔ 242By similarity
    Disulfide bondi236 ↔ 255By similarity
    Disulfide bondi249 ↔ 266By similarity
    Disulfide bondi270 ↔ 298By similarity
    Glycosylationi373 – 3731N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi380 – 3801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi515 – 5151N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi569 ↔ 597By similarity
    Glycosylationi579 – 5791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi675 – 6751N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi688 ↔ 700By similarity
    Disulfide bondi695 ↔ 713By similarity
    Disulfide bondi707 ↔ 722By similarity
    Glycosylationi727 – 7271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi751 – 7511N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi770 – 7701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi791 – 7911N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi802 ↔ 812By similarity
    Disulfide bondi817 ↔ 945Interchain (between heavy and light chains)PROSITE-ProRule annotation
    Disulfide bondi859 ↔ 875By similarity
    Glycosylationi897 – 8971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi936 – 9361N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi959 ↔ 1027By similarity
    Disulfide bondi991 ↔ 1006By similarity
    Glycosylationi999 – 9991N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi1017 ↔ 1045By similarity

    Post-translational modificationi

    The chains are derived from a single precursor that is cleaved by a trypsin-like protease.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiP97435.
    PaxDbiP97435.
    PRIDEiP97435.

    PTM databases

    PhosphoSiteiP97435.

    Expressioni

    Gene expression databases

    BgeeiP97435.
    CleanExiMM_PRSS7.
    GenevestigatoriP97435.

    Interactioni

    Subunit structurei

    Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP97435.
    SMRiP97435. Positions 392-547, 568-704, 830-1064.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1818CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini48 – 10691022ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei19 – 4729Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini54 – 169116SEAPROSITE-ProRule annotationAdd
    BLAST
    Domaini227 – 26842LDL-receptor class A 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini270 – 379110CUB 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini387 – 549163MAMPROSITE-ProRule annotationAdd
    BLAST
    Domaini569 – 679111CUB 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini686 – 72439LDL-receptor class A 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini723 – 81694SRCRPROSITE-ProRule annotationAdd
    BLAST
    Domaini830 – 1069240Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 2 CUB domains.PROSITE-ProRule annotation
    Contains 2 LDL-receptor class A domains.PROSITE-ProRule annotation
    Contains 1 MAM domain.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
    Contains 1 SEA domain.PROSITE-ProRule annotation
    Contains 1 SRCR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00720000108393.
    HOGENOMiHOG000112380.
    HOVERGENiHBG005588.
    InParanoidiQ148Y3.
    KOiK01316.
    OMAiFEDGFCF.
    OrthoDBiEOG7RNK07.
    PhylomeDBiP97435.
    TreeFamiTF351678.

    Family and domain databases

    Gene3Di2.60.120.290. 2 hits.
    3.10.250.10. 1 hit.
    3.30.70.960. 1 hit.
    4.10.400.10. 2 hits.
    InterProiIPR008985. ConA-like_lec_gl_sf.
    IPR000859. CUB_dom.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR000998. MAM_dom.
    IPR011163. Pept_S1A_enterop.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR000082. SEA_dom.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00431. CUB. 2 hits.
    PF00057. Ldl_recept_a. 1 hit.
    PF00629. MAM. 1 hit.
    PF01390. SEA. 1 hit.
    PF00530. SRCR. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001138. Enteropeptidase. 1 hit.
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00042. CUB. 2 hits.
    SM00192. LDLa. 2 hits.
    SM00137. MAM. 1 hit.
    SM00200. SEA. 1 hit.
    SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF49854. SSF49854. 2 hits.
    SSF49899. SSF49899. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    SSF57424. SSF57424. 2 hits.
    SSF82671. SSF82671. 1 hit.
    PROSITEiPS01180. CUB. 2 hits.
    PS01209. LDLRA_1. 2 hits.
    PS50068. LDLRA_2. 2 hits.
    PS00740. MAM_1. 1 hit.
    PS50060. MAM_2. 1 hit.
    PS50024. SEA. 1 hit.
    PS00420. SRCR_1. 1 hit.
    PS50287. SRCR_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97435-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKSSRDEAVG HHSISSFEVM LSALFIMLMV FSIGLIAVSW LAVKESEGDA     50
    ALGKSHEVRG TFKITSGVTY NPNLQDKHSV DFKVLAFDLQ QMIDEIFESS 100
    SLKNEYEKSK VFQFEKGSVI VLFDLFFAQW VSDKNVKEEL IQGIEANISS 150
    QLVTLHIDLN SIDITASLSD FTTAVPVTTS DKLTTSSPMT TSASLGNLST 200
    TVAATTSAPL CNLSTATFAT TSGHVSIECQ PGSRPCAHAW NCVATDLFCD 250
    GEVNCPDGSD EDTGLCATAC DGRFLLTGDS GVFQADRYPR PDESGVVCRW 300
    IIRVNQGLSI RMNFGSFIPH YTDVLDIYEG IGPSKILRGS FWETDPGTIR 350
    IFSNLVTVTF LIKSDEYDYI GFNATYSTFN NSELNNYEKI DCTFDDGFCF 400
    WTQDLDDDNE WERIQVTTFP CYTGPRFDHT YGNGSGFYIS TPTEQGWRSE 450
    RVGLSSLSLD LTSEPVCLHF WYYMCCENVY NLNIHISSAE TTDKIVFQRK 500
    GNYGRNWNYG QVTLNETGEF KVVFNAFRNR GCSTIALDDI SLTNGICSQS 550
    PYPEPTLVPT PPPELPTDCG GPFELWEPNS TFSSPNFPDK YPNQASCIWN 600
    LNAQRGKNIQ LHFQEFDLEN INDVVEVRDG GEFDSLLLAV YTGPGPVKDL 650
    FSTTNRMTVI FTTNMETRRK GFKANFTSGY YLGIPEPCQD DEFQCKDGNC 700
    IPLGNLCDSY PHCRDGSDEA SCVRFLNGTR SNNGLVQFNI HSIWHIACAE 750
    NWTTQISNEV CHLLGLGSAN SSMPISSTGG GPFVRVNQAP NGSLILTPSL 800
    QCSQDSLILL QCNHKSCGEK KVTQKVSPKI VGGSDAQAGA WPWVVALYHR 850
    DRSTDRLLCG ASLVSSDWLV SAAHCVYRRN LDPTRWTAVL GLHMQSNLTS 900
    PQVVRRVVDQ IVINPHYDRR RKVNDIAMMH LEFKVNYTDY IQPICLPEEN 950
    QIFIPGRTCS IAGWGYDKIN AGSTVDVLKE ADVPLISNEK CQQQLPEYNI 1000
    TESMICAGYE EGGIDSCQGD SGGPLMCQEN NRWFLVGVTS FGVQCALPNH 1050
    PGVYVRVSQF IEWIHSFLH 1069
    Length:1,069
    Mass (Da):118,735
    Last modified:May 1, 1997 - v1
    Checksum:iE62549E463743C3D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73378 mRNA. Translation: AAB37317.1.
    BC117917 mRNA. Translation: AAI17918.1.
    BC117918 mRNA. Translation: AAI17919.1.
    CCDSiCCDS28280.1.
    RefSeqiNP_032967.1. NM_008941.3.
    NP_849186.2. NM_178855.4.
    UniGeneiMm.5184.

    Genome annotation databases

    EnsembliENSMUST00000023566; ENSMUSP00000023566; ENSMUSG00000022857.
    GeneIDi19146.
    KEGGimmu:19146.
    UCSCiuc007zsy.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U73378 mRNA. Translation: AAB37317.1 .
    BC117917 mRNA. Translation: AAI17918.1 .
    BC117918 mRNA. Translation: AAI17919.1 .
    CCDSi CCDS28280.1.
    RefSeqi NP_032967.1. NM_008941.3.
    NP_849186.2. NM_178855.4.
    UniGenei Mm.5184.

    3D structure databases

    ProteinModelPortali P97435.
    SMRi P97435. Positions 392-547, 568-704, 830-1064.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S01.156.

    PTM databases

    PhosphoSitei P97435.

    Proteomic databases

    MaxQBi P97435.
    PaxDbi P97435.
    PRIDEi P97435.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023566 ; ENSMUSP00000023566 ; ENSMUSG00000022857 .
    GeneIDi 19146.
    KEGGi mmu:19146.
    UCSCi uc007zsy.1. mouse.

    Organism-specific databases

    CTDi 5651.
    MGIi MGI:1197523. Tmprss15.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00720000108393.
    HOGENOMi HOG000112380.
    HOVERGENi HBG005588.
    InParanoidi Q148Y3.
    KOi K01316.
    OMAi FEDGFCF.
    OrthoDBi EOG7RNK07.
    PhylomeDBi P97435.
    TreeFami TF351678.

    Miscellaneous databases

    NextBioi 295786.
    PROi P97435.
    SOURCEi Search...

    Gene expression databases

    Bgeei P97435.
    CleanExi MM_PRSS7.
    Genevestigatori P97435.

    Family and domain databases

    Gene3Di 2.60.120.290. 2 hits.
    3.10.250.10. 1 hit.
    3.30.70.960. 1 hit.
    4.10.400.10. 2 hits.
    InterProi IPR008985. ConA-like_lec_gl_sf.
    IPR000859. CUB_dom.
    IPR023415. LDLR_class-A_CS.
    IPR002172. LDrepeatLR_classA_rpt.
    IPR000998. MAM_dom.
    IPR011163. Pept_S1A_enterop.
    IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR000082. SEA_dom.
    IPR001190. SRCR.
    IPR017448. SRCR-like_dom.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00431. CUB. 2 hits.
    PF00057. Ldl_recept_a. 1 hit.
    PF00629. MAM. 1 hit.
    PF01390. SEA. 1 hit.
    PF00530. SRCR. 1 hit.
    PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001138. Enteropeptidase. 1 hit.
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00042. CUB. 2 hits.
    SM00192. LDLa. 2 hits.
    SM00137. MAM. 1 hit.
    SM00200. SEA. 1 hit.
    SM00202. SR. 1 hit.
    SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49854. SSF49854. 2 hits.
    SSF49899. SSF49899. 1 hit.
    SSF50494. SSF50494. 1 hit.
    SSF56487. SSF56487. 1 hit.
    SSF57424. SSF57424. 2 hits.
    SSF82671. SSF82671. 1 hit.
    PROSITEi PS01180. CUB. 2 hits.
    PS01209. LDLRA_1. 2 hits.
    PS50068. LDLRA_2. 2 hits.
    PS00740. MAM_1. 1 hit.
    PS50060. MAM_2. 1 hit.
    PS50024. SEA. 1 hit.
    PS00420. SRCR_1. 1 hit.
    PS50287. SRCR_2. 1 hit.
    PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of murine enterokinase (enteropeptidase) and expression in small intestine during development."
      Yuan X., Zheng X., Lu D., Rubin D.C., Pung C.Y.M., Sadler J.E.
      Am. J. Physiol. 274:G342-G349(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
      Tissue: Duodenum.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].

    Entry informationi

    Entry nameiENTK_MOUSE
    AccessioniPrimary (citable) accession number: P97435
    Secondary accession number(s): Q148Y3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3