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Protein

Enteropeptidase

Gene

Tmprss15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases (By similarity).By similarity

Catalytic activityi

Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei874Charge relay systemBy similarity1
Active sitei925Charge relay systemBy similarity1
Active sitei1021Charge relay systemBy similarity1

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.156

Names & Taxonomyi

Protein namesi
Recommended name:
Enteropeptidase (EC:3.4.21.9)
Alternative name(s):
Enterokinase
Serine protease 7
Transmembrane protease serine 15
Cleaved into the following 2 chains:
Gene namesi
Name:Tmprss15
Synonyms:Entk, Prss7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1197523 Tmprss15

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 47Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST29
Topological domaini48 – 1069ExtracellularSequence analysisAdd BLAST1022

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000277211 – 829Enteropeptidase non-catalytic heavy chainAdd BLAST829
ChainiPRO_0000027722830 – 1069Enteropeptidase catalytic light chainAdd BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi147N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi197N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi212N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi229 ↔ 242By similarity
Disulfide bondi236 ↔ 255By similarity
Disulfide bondi249 ↔ 266By similarity
Disulfide bondi270 ↔ 298By similarity
Glycosylationi373N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi380N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi433N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi515N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi569 ↔ 597By similarity
Glycosylationi579N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi675N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi688 ↔ 700By similarity
Disulfide bondi695 ↔ 713By similarity
Disulfide bondi707 ↔ 722By similarity
Glycosylationi727N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi751N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi770N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi791N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi802 ↔ 812By similarity
Disulfide bondi817 ↔ 945Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi859 ↔ 875By similarity
Glycosylationi897N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi936N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi959 ↔ 1027By similarity
Disulfide bondi991 ↔ 1006By similarity
Glycosylationi999N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi1017 ↔ 1045By similarity

Post-translational modificationi

The chains are derived from a single precursor that is cleaved by a trypsin-like protease.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP97435
PaxDbiP97435
PRIDEiP97435

PTM databases

iPTMnetiP97435
PhosphoSitePlusiP97435

Expressioni

Gene expression databases

BgeeiENSMUSG00000022857
CleanExiMM_PRSS7
ExpressionAtlasiP97435 baseline and differential
GenevisibleiP97435 MM

Interactioni

Subunit structurei

Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023566

Structurei

3D structure databases

ProteinModelPortaliP97435
SMRiP97435
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 169SEAPROSITE-ProRule annotationAdd BLAST116
Domaini227 – 268LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST42
Domaini270 – 379CUB 1PROSITE-ProRule annotationAdd BLAST110
Domaini387 – 549MAMPROSITE-ProRule annotationAdd BLAST163
Domaini569 – 679CUB 2PROSITE-ProRule annotationAdd BLAST111
Domaini686 – 724LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST39
Domaini723 – 816SRCRPROSITE-ProRule annotationAdd BLAST94
Domaini830 – 1069Peptidase S1PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
GeneTreeiENSGT00760000118962
HOGENOMiHOG000112380
HOVERGENiHBG005588
InParanoidiP97435
KOiK01316
OMAiNWNYGQV
OrthoDBiEOG091G0DF7
PhylomeDBiP97435
TreeFamiTF351678

Family and domain databases

CDDicd00041 CUB, 2 hits
cd00112 LDLa, 2 hits
cd06263 MAM, 1 hit
cd00190 Tryp_SPc, 1 hit
Gene3Di2.60.120.290, 2 hits
3.10.250.10, 1 hit
3.30.70.960, 1 hit
InterProiView protein in InterPro
IPR013320 ConA-like_dom_sf
IPR000859 CUB_dom
IPR036055 LDL_receptor-like_sf
IPR023415 LDLR_class-A_CS
IPR002172 LDrepeatLR_classA_rpt
IPR000998 MAM_dom
IPR011163 Pept_S1A_enterop
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR000082 SEA_dom
IPR036364 SEA_dom_sf
IPR035914 Sperma_CUB_dom_sf
IPR001190 SRCR
IPR017448 SRCR-like_dom
IPR036772 SRCR-like_dom_sf
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00431 CUB, 2 hits
PF00057 Ldl_recept_a, 1 hit
PF00629 MAM, 1 hit
PF01390 SEA, 1 hit
PF00530 SRCR, 1 hit
PF00089 Trypsin, 1 hit
PIRSFiPIRSF001138 Enteropeptidase, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00042 CUB, 2 hits
SM00192 LDLa, 2 hits
SM00137 MAM, 1 hit
SM00200 SEA, 1 hit
SM00202 SR, 1 hit
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF49854 SSF49854, 2 hits
SSF49899 SSF49899, 1 hit
SSF50494 SSF50494, 1 hit
SSF56487 SSF56487, 1 hit
SSF57424 SSF57424, 2 hits
SSF82671 SSF82671, 1 hit
PROSITEiView protein in PROSITE
PS01180 CUB, 2 hits
PS01209 LDLRA_1, 2 hits
PS50068 LDLRA_2, 2 hits
PS00740 MAM_1, 1 hit
PS50060 MAM_2, 1 hit
PS50024 SEA, 1 hit
PS00420 SRCR_1, 1 hit
PS50287 SRCR_2, 1 hit
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97435-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSSRDEAVG HHSISSFEVM LSALFIMLMV FSIGLIAVSW LAVKESEGDA
60 70 80 90 100
ALGKSHEVRG TFKITSGVTY NPNLQDKHSV DFKVLAFDLQ QMIDEIFESS
110 120 130 140 150
SLKNEYEKSK VFQFEKGSVI VLFDLFFAQW VSDKNVKEEL IQGIEANISS
160 170 180 190 200
QLVTLHIDLN SIDITASLSD FTTAVPVTTS DKLTTSSPMT TSASLGNLST
210 220 230 240 250
TVAATTSAPL CNLSTATFAT TSGHVSIECQ PGSRPCAHAW NCVATDLFCD
260 270 280 290 300
GEVNCPDGSD EDTGLCATAC DGRFLLTGDS GVFQADRYPR PDESGVVCRW
310 320 330 340 350
IIRVNQGLSI RMNFGSFIPH YTDVLDIYEG IGPSKILRGS FWETDPGTIR
360 370 380 390 400
IFSNLVTVTF LIKSDEYDYI GFNATYSTFN NSELNNYEKI DCTFDDGFCF
410 420 430 440 450
WTQDLDDDNE WERIQVTTFP CYTGPRFDHT YGNGSGFYIS TPTEQGWRSE
460 470 480 490 500
RVGLSSLSLD LTSEPVCLHF WYYMCCENVY NLNIHISSAE TTDKIVFQRK
510 520 530 540 550
GNYGRNWNYG QVTLNETGEF KVVFNAFRNR GCSTIALDDI SLTNGICSQS
560 570 580 590 600
PYPEPTLVPT PPPELPTDCG GPFELWEPNS TFSSPNFPDK YPNQASCIWN
610 620 630 640 650
LNAQRGKNIQ LHFQEFDLEN INDVVEVRDG GEFDSLLLAV YTGPGPVKDL
660 670 680 690 700
FSTTNRMTVI FTTNMETRRK GFKANFTSGY YLGIPEPCQD DEFQCKDGNC
710 720 730 740 750
IPLGNLCDSY PHCRDGSDEA SCVRFLNGTR SNNGLVQFNI HSIWHIACAE
760 770 780 790 800
NWTTQISNEV CHLLGLGSAN SSMPISSTGG GPFVRVNQAP NGSLILTPSL
810 820 830 840 850
QCSQDSLILL QCNHKSCGEK KVTQKVSPKI VGGSDAQAGA WPWVVALYHR
860 870 880 890 900
DRSTDRLLCG ASLVSSDWLV SAAHCVYRRN LDPTRWTAVL GLHMQSNLTS
910 920 930 940 950
PQVVRRVVDQ IVINPHYDRR RKVNDIAMMH LEFKVNYTDY IQPICLPEEN
960 970 980 990 1000
QIFIPGRTCS IAGWGYDKIN AGSTVDVLKE ADVPLISNEK CQQQLPEYNI
1010 1020 1030 1040 1050
TESMICAGYE EGGIDSCQGD SGGPLMCQEN NRWFLVGVTS FGVQCALPNH
1060
PGVYVRVSQF IEWIHSFLH
Length:1,069
Mass (Da):118,735
Last modified:May 1, 1997 - v1
Checksum:iE62549E463743C3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73378 mRNA Translation: AAB37317.1
BC117917 mRNA Translation: AAI17918.1
BC117918 mRNA Translation: AAI17919.1
CCDSiCCDS28280.1
RefSeqiNP_032967.1, NM_008941.3
NP_849186.2, NM_178855.4
UniGeneiMm.5184

Genome annotation databases

EnsembliENSMUST00000023566; ENSMUSP00000023566; ENSMUSG00000022857
GeneIDi19146
KEGGimmu:19146
UCSCiuc007zsy.2 mouse

Similar proteinsi

Entry informationi

Entry nameiENTK_MOUSE
AccessioniPrimary (citable) accession number: P97435
Secondary accession number(s): Q148Y3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: March 28, 2018
This is version 159 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

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