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Protein

Enteropeptidase

Gene

Tmprss15

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Responsible for initiating activation of pancreatic proteolytic proenzymes (trypsin, chymotrypsin and carboxypeptidase A). It catalyzes the conversion of trypsinogen to trypsin which in turn activates other proenzymes including chymotrypsinogen, procarboxypeptidases, and proelastases (By similarity).By similarity

Catalytic activityi

Activation of trypsinogen by selective cleavage of 6-Lys-|-Ile-7 bond.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei874Charge relay systemBy similarity1
Active sitei925Charge relay systemBy similarity1
Active sitei1021Charge relay systemBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.156.

Names & Taxonomyi

Protein namesi
Recommended name:
Enteropeptidase (EC:3.4.21.9)
Alternative name(s):
Enterokinase
Serine protease 7
Transmembrane protease serine 15
Cleaved into the following 2 chains:
Gene namesi
Name:Tmprss15
Synonyms:Entk, Prss7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1197523. Tmprss15.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 18CytoplasmicSequence analysisAdd BLAST18
Transmembranei19 – 47Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST29
Topological domaini48 – 1069ExtracellularSequence analysisAdd BLAST1022

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000277211 – 829Enteropeptidase non-catalytic heavy chainAdd BLAST829
ChainiPRO_0000027722830 – 1069Enteropeptidase catalytic light chainAdd BLAST240

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi147N-linked (GlcNAc...)Sequence analysis1
Glycosylationi197N-linked (GlcNAc...)Sequence analysis1
Glycosylationi212N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi229 ↔ 242By similarity
Disulfide bondi236 ↔ 255By similarity
Disulfide bondi249 ↔ 266By similarity
Disulfide bondi270 ↔ 298By similarity
Glycosylationi373N-linked (GlcNAc...)Sequence analysis1
Glycosylationi380N-linked (GlcNAc...)Sequence analysis1
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Glycosylationi515N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi569 ↔ 597By similarity
Glycosylationi579N-linked (GlcNAc...)Sequence analysis1
Glycosylationi675N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi688 ↔ 700By similarity
Disulfide bondi695 ↔ 713By similarity
Disulfide bondi707 ↔ 722By similarity
Glycosylationi727N-linked (GlcNAc...)Sequence analysis1
Glycosylationi751N-linked (GlcNAc...)Sequence analysis1
Glycosylationi770N-linked (GlcNAc...)Sequence analysis1
Glycosylationi791N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi802 ↔ 812By similarity
Disulfide bondi817 ↔ 945Interchain (between heavy and light chains)PROSITE-ProRule annotation
Disulfide bondi859 ↔ 875By similarity
Glycosylationi897N-linked (GlcNAc...)Sequence analysis1
Glycosylationi936N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi959 ↔ 1027By similarity
Disulfide bondi991 ↔ 1006By similarity
Glycosylationi999N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi1017 ↔ 1045By similarity

Post-translational modificationi

The chains are derived from a single precursor that is cleaved by a trypsin-like protease.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiP97435.
PaxDbiP97435.
PRIDEiP97435.

PTM databases

PhosphoSitePlusiP97435.

Expressioni

Gene expression databases

BgeeiENSMUSG00000022857.
CleanExiMM_PRSS7.
ExpressionAtlasiP97435. baseline and differential.
GenevisibleiP97435. MM.

Interactioni

Subunit structurei

Heterodimer of a catalytic (light) chain and a multidomain (heavy) chain linked by a disulfide bond.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023566.

Structurei

3D structure databases

ProteinModelPortaliP97435.
SMRiP97435.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini54 – 169SEAPROSITE-ProRule annotationAdd BLAST116
Domaini227 – 268LDL-receptor class A 1PROSITE-ProRule annotationAdd BLAST42
Domaini270 – 379CUB 1PROSITE-ProRule annotationAdd BLAST110
Domaini387 – 549MAMPROSITE-ProRule annotationAdd BLAST163
Domaini569 – 679CUB 2PROSITE-ProRule annotationAdd BLAST111
Domaini686 – 724LDL-receptor class A 2PROSITE-ProRule annotationAdd BLAST39
Domaini723 – 816SRCRPROSITE-ProRule annotationAdd BLAST94
Domaini830 – 1069Peptidase S1PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 2 CUB domains.PROSITE-ProRule annotation
Contains 2 LDL-receptor class A domains.PROSITE-ProRule annotation
Contains 1 MAM domain.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation
Contains 1 SEA domain.PROSITE-ProRule annotation
Contains 1 SRCR domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000112380.
HOVERGENiHBG005588.
InParanoidiP97435.
KOiK01316.
OMAiFEDGFCF.
OrthoDBiEOG091G0DF7.
PhylomeDBiP97435.
TreeFamiTF351678.

Family and domain databases

CDDicd00041. CUB. 2 hits.
cd06263. MAM. 1 hit.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
3.10.250.10. 1 hit.
3.30.70.960. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR000998. MAM_dom.
IPR011163. Pept_S1A_enterop.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000082. SEA_dom.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF00629. MAM. 1 hit.
PF01390. SEA. 1 hit.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001138. Enteropeptidase. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 2 hits.
SM00137. MAM. 1 hit.
SM00200. SEA. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 2 hits.
PS50068. LDLRA_2. 2 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50024. SEA. 1 hit.
PS00420. SRCR_1. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97435-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKSSRDEAVG HHSISSFEVM LSALFIMLMV FSIGLIAVSW LAVKESEGDA
60 70 80 90 100
ALGKSHEVRG TFKITSGVTY NPNLQDKHSV DFKVLAFDLQ QMIDEIFESS
110 120 130 140 150
SLKNEYEKSK VFQFEKGSVI VLFDLFFAQW VSDKNVKEEL IQGIEANISS
160 170 180 190 200
QLVTLHIDLN SIDITASLSD FTTAVPVTTS DKLTTSSPMT TSASLGNLST
210 220 230 240 250
TVAATTSAPL CNLSTATFAT TSGHVSIECQ PGSRPCAHAW NCVATDLFCD
260 270 280 290 300
GEVNCPDGSD EDTGLCATAC DGRFLLTGDS GVFQADRYPR PDESGVVCRW
310 320 330 340 350
IIRVNQGLSI RMNFGSFIPH YTDVLDIYEG IGPSKILRGS FWETDPGTIR
360 370 380 390 400
IFSNLVTVTF LIKSDEYDYI GFNATYSTFN NSELNNYEKI DCTFDDGFCF
410 420 430 440 450
WTQDLDDDNE WERIQVTTFP CYTGPRFDHT YGNGSGFYIS TPTEQGWRSE
460 470 480 490 500
RVGLSSLSLD LTSEPVCLHF WYYMCCENVY NLNIHISSAE TTDKIVFQRK
510 520 530 540 550
GNYGRNWNYG QVTLNETGEF KVVFNAFRNR GCSTIALDDI SLTNGICSQS
560 570 580 590 600
PYPEPTLVPT PPPELPTDCG GPFELWEPNS TFSSPNFPDK YPNQASCIWN
610 620 630 640 650
LNAQRGKNIQ LHFQEFDLEN INDVVEVRDG GEFDSLLLAV YTGPGPVKDL
660 670 680 690 700
FSTTNRMTVI FTTNMETRRK GFKANFTSGY YLGIPEPCQD DEFQCKDGNC
710 720 730 740 750
IPLGNLCDSY PHCRDGSDEA SCVRFLNGTR SNNGLVQFNI HSIWHIACAE
760 770 780 790 800
NWTTQISNEV CHLLGLGSAN SSMPISSTGG GPFVRVNQAP NGSLILTPSL
810 820 830 840 850
QCSQDSLILL QCNHKSCGEK KVTQKVSPKI VGGSDAQAGA WPWVVALYHR
860 870 880 890 900
DRSTDRLLCG ASLVSSDWLV SAAHCVYRRN LDPTRWTAVL GLHMQSNLTS
910 920 930 940 950
PQVVRRVVDQ IVINPHYDRR RKVNDIAMMH LEFKVNYTDY IQPICLPEEN
960 970 980 990 1000
QIFIPGRTCS IAGWGYDKIN AGSTVDVLKE ADVPLISNEK CQQQLPEYNI
1010 1020 1030 1040 1050
TESMICAGYE EGGIDSCQGD SGGPLMCQEN NRWFLVGVTS FGVQCALPNH
1060
PGVYVRVSQF IEWIHSFLH
Length:1,069
Mass (Da):118,735
Last modified:May 1, 1997 - v1
Checksum:iE62549E463743C3D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73378 mRNA. Translation: AAB37317.1.
BC117917 mRNA. Translation: AAI17918.1.
BC117918 mRNA. Translation: AAI17919.1.
CCDSiCCDS28280.1.
RefSeqiNP_032967.1. NM_008941.3.
NP_849186.2. NM_178855.4.
UniGeneiMm.5184.

Genome annotation databases

EnsembliENSMUST00000023566; ENSMUSP00000023566; ENSMUSG00000022857.
GeneIDi19146.
KEGGimmu:19146.
UCSCiuc007zsy.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73378 mRNA. Translation: AAB37317.1.
BC117917 mRNA. Translation: AAI17918.1.
BC117918 mRNA. Translation: AAI17919.1.
CCDSiCCDS28280.1.
RefSeqiNP_032967.1. NM_008941.3.
NP_849186.2. NM_178855.4.
UniGeneiMm.5184.

3D structure databases

ProteinModelPortaliP97435.
SMRiP97435.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000023566.

Protein family/group databases

MEROPSiS01.156.

PTM databases

PhosphoSitePlusiP97435.

Proteomic databases

MaxQBiP97435.
PaxDbiP97435.
PRIDEiP97435.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023566; ENSMUSP00000023566; ENSMUSG00000022857.
GeneIDi19146.
KEGGimmu:19146.
UCSCiuc007zsy.2. mouse.

Organism-specific databases

CTDi5651.
MGIiMGI:1197523. Tmprss15.

Phylogenomic databases

eggNOGiKOG3627. Eukaryota.
COG5640. LUCA.
GeneTreeiENSGT00760000118962.
HOGENOMiHOG000112380.
HOVERGENiHBG005588.
InParanoidiP97435.
KOiK01316.
OMAiFEDGFCF.
OrthoDBiEOG091G0DF7.
PhylomeDBiP97435.
TreeFamiTF351678.

Miscellaneous databases

PROiP97435.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022857.
CleanExiMM_PRSS7.
ExpressionAtlasiP97435. baseline and differential.
GenevisibleiP97435. MM.

Family and domain databases

CDDicd00041. CUB. 2 hits.
cd06263. MAM. 1 hit.
cd00190. Tryp_SPc. 1 hit.
Gene3Di2.60.120.290. 2 hits.
3.10.250.10. 1 hit.
3.30.70.960. 1 hit.
4.10.400.10. 2 hits.
InterProiIPR013320. ConA-like_dom.
IPR000859. CUB_dom.
IPR023415. LDLR_class-A_CS.
IPR002172. LDrepeatLR_classA_rpt.
IPR000998. MAM_dom.
IPR011163. Pept_S1A_enterop.
IPR009003. Peptidase_S1_PA.
IPR001314. Peptidase_S1A.
IPR000082. SEA_dom.
IPR001190. SRCR.
IPR017448. SRCR-like_dom.
IPR001254. Trypsin_dom.
IPR018114. TRYPSIN_HIS.
IPR033116. TRYPSIN_SER.
[Graphical view]
PfamiPF00431. CUB. 2 hits.
PF00057. Ldl_recept_a. 1 hit.
PF00629. MAM. 1 hit.
PF01390. SEA. 1 hit.
PF00530. SRCR. 1 hit.
PF00089. Trypsin. 1 hit.
[Graphical view]
PIRSFiPIRSF001138. Enteropeptidase. 1 hit.
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00042. CUB. 2 hits.
SM00192. LDLa. 2 hits.
SM00137. MAM. 1 hit.
SM00200. SEA. 1 hit.
SM00202. SR. 1 hit.
SM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
SSF50494. SSF50494. 1 hit.
SSF56487. SSF56487. 1 hit.
SSF57424. SSF57424. 2 hits.
SSF82671. SSF82671. 1 hit.
PROSITEiPS01180. CUB. 2 hits.
PS01209. LDLRA_1. 2 hits.
PS50068. LDLRA_2. 2 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
PS50024. SEA. 1 hit.
PS00420. SRCR_1. 1 hit.
PS50287. SRCR_2. 1 hit.
PS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENTK_MOUSE
AccessioniPrimary (citable) accession number: P97435
Secondary accession number(s): Q148Y3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 151 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.