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Protein

Myosin phosphatase Rho-interacting protein

Gene

Mprip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.2 Publications

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin phosphatase Rho-interacting protein
Alternative name(s):
Rho-interacting protein 3
Short name:
RIP3
p116Rip
Gene namesi
Name:Mprip
Synonyms:Kiaa0864, Mrip, Rhoip3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1349438. Mprip.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi857 – 8571L → P: Loss of interaction with PPP1R12C and PPP1R12A. 1 Publication
Mutagenesisi905 – 9051L → P: No effect. 1 Publication
Mutagenesisi919 – 9191I → P: Loss of interaction with PPP1R12C and PPP1R12A. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10241024Myosin phosphatase Rho-interacting proteinPRO_0000223870Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei193 – 1931PhosphoserineBy similarity
Modified residuei219 – 2191PhosphoserineCombined sources
Modified residuei221 – 2211PhosphoserineCombined sources
Modified residuei225 – 2251PhosphoserineCombined sources
Modified residuei227 – 2271PhosphoserineCombined sources
Modified residuei266 – 2661PhosphoserineBy similarity
Modified residuei270 – 2701PhosphoserineBy similarity
Modified residuei289 – 2891PhosphoserineCombined sources
Modified residuei292 – 2921PhosphoserineBy similarity
Modified residuei295 – 2951PhosphothreonineBy similarity
Modified residuei364 – 3641PhosphoserineCombined sources
Modified residuei492 – 4921PhosphoserineBy similarity
Modified residuei617 – 6171PhosphoserineCombined sources
Modified residuei645 – 6451PhosphothreonineBy similarity
Modified residuei799 – 7991PhosphoserineBy similarity
Modified residuei976 – 9761PhosphoserineCombined sources
Modified residuei992 – 9921PhosphoserineBy similarity
Modified residuei1013 – 10131PhosphoserineCombined sources
Modified residuei1015 – 10151PhosphoserineCombined sources
Isoform 3 (identifier: P97434-3)
Modified residuei979 – 9791PhosphoserineCombined sources
Isoform 2 (identifier: P97434-2)
Modified residuei1015 – 10151PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP97434.
PaxDbiP97434.
PeptideAtlasiP97434.
PRIDEiP97434.

PTM databases

iPTMnetiP97434.
PhosphoSiteiP97434.

Expressioni

Tissue specificityi

Expressed in Kidney, Brain, Heart and Lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000005417.
ExpressionAtlasiP97434. baseline and differential.
GenevisibleiP97434. MM.

Interactioni

Subunit structurei

Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains. Interacts with MYZAP. Binds F-actin through its N-terminus.4 Publications

Protein-protein interaction databases

BioGridi205073. 7 interactions.
IntActiP97434. 10 interactions.
MINTiMINT-4132519.
STRINGi10090.ENSMUSP00000071914.

Structurei

3D structure databases

ProteinModelPortaliP97434.
SMRiP97434. Positions 390-481.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini43 – 150108PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini386 – 48297PH 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 382382Interaction with F-actinAdd
BLAST
Regioni545 – 823279Interaction with RHOAAdd
BLAST
Regioni823 – 87856Interaction with PPP1R12ABy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili672 – 976305Sequence analysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi184 – 1907Poly-Ser

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG4807. Eukaryota.
ENOG410ZA8F. LUCA.
GeneTreeiENSGT00530000063678.
HOVERGENiHBG023864.
InParanoidiP97434.
TreeFamiTF329258.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97434-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL
60 70 80 90 100
LLAPDGTDFD NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM
110 120 130 140 150
NQCTDVVDGE ARTGQKFSLC ILTPDKEHFI RAETKEIISG WLEMLMVYPR
160 170 180 190 200
TNKQNQKKKR KVEPPTPQEP GPAKMAVTSS SGGSSGSSSS IPSAEKVPTT
210 220 230 240 250
KSTLWQEEMR AKDQPDGTSL SPAQSPSQSQ PPAACTPREP GLESKEDEST
260 270 280 290 300
ISGDRVDGGR KVRVESGYFS LEKAKQDLRA EEQLPPLLSP PSPSTPHSRR
310 320 330 340 350
SQVIEKFEAL DIEKAEHMET NMLILTTPSS DTRQGRSERR AIPRKRDFAS
360 370 380 390 400
EAPTAPLSDA CPLSPHRRAK SLDRRSTESS MTPDLLNFKK GWLTKQYEDG
410 420 430 440 450
QWKKHWFVLA DQSLRYYRDS VAEEAADLDG EINLSTCYDV TEYPVQRNYG
460 470 480 490 500
FQIHTKEGEF TLSAMTSGIR RNWIQTIMKH VLPASAPDVT SSLPEGKNKS
510 520 530 540 550
TSFETCSRST EKQEAEPGEP DPEQKKSRAR ERRREGRSKT FDWAEFRPIQ
560 570 580 590 600
QALAQERASA VGSSDSGDPG CLEAEPGELE RERARRREER RKRFGMLDTI
610 620 630 640 650
DGPGMEDTAL RMDIDRSPGL LGTPDLKTQN VHVEIEQRWH QVETTPLREE
660 670 680 690 700
KQVPIAPLHL SLEDRSERLS THELTSLLEK ELEQSQKEAS DLLEQNRLLQ
710 720 730 740 750
DQLRVALGRE QSAREGYVLQ ATCERGFAAM EETHQKKIED LQRQHQRELE
760 770 780 790 800
KLREEKDRLL AEETAATISA IEAMKNAHRE EMERELEKSQ RSQISSINSD
810 820 830 840 850
IEALRRQYLE ELQSVQRELE VLSEQYSQKC LENAHLAQAL EAERQALRQC
860 870 880 890 900
QRENQELNAH NQELNNRLAA EITRLRTLLT GDGGGESTGL PLTQGKDAYE
910 920 930 940 950
LEVLLRVKES EIQYLKQEIS SLKDELQTAL RDKKYASDKY KDIYTELSIA
960 970 980 990 1000
KAKADCDISR LKEQLKAATE ALGEKSPEGT TVSGYDIMKS KSNPDFLKKD
1010 1020
RSCVTRQLRN IRSKSVIEQV SWDN
Length:1,024
Mass (Da):116,408
Last modified:February 21, 2006 - v2
Checksum:i7BE3BFB89011ECC5
GO
Isoform 2 (identifier: P97434-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1016-1024: VIEQVSWDN → LKEGLTVQERLKLFESRDLKKD

Show »
Length:1,037
Mass (Da):117,965
Checksum:i44AD4103995E08F7
GO
Isoform 3 (identifier: P97434-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-382: Missing.
     1016-1024: VIEQVSWDN → LKEGLTVQERLKLFESRDLKKD

Note: Gene prediction based on human cDNA data. No experimental confirmation available.Combined sources
Show »
Length:1,001
Mass (Da):114,094
Checksum:iC718480566C25CA9
GO
Isoform 4 (identifier: P97434-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-205: Missing.

Note: No experimental confirmation available.
Show »
Length:986
Mass (Da):112,703
Checksum:iF8F9FF6AA58B6330
GO
Isoform 5 (identifier: P97434-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     402-409: WKKHWFVL → TQVPFLRTKIGTF

Note: No experimental confirmation available.
Show »
Length:1,029
Mass (Da):116,773
Checksum:iB14325EC3B88E5A5
GO

Sequence cautioni

The sequence BAE36957 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti43 – 431K → E in BAE32843 (PubMed:16141072).Curated
Sequence conflicti70 – 701Q → R in BAE33355 (PubMed:16141072).Curated
Sequence conflicti184 – 1841S → T in AAB18198 (PubMed:9199174).Curated
Sequence conflicti289 – 2891S → P in BAE42242 (PubMed:16141072).Curated
Sequence conflicti301 – 3011S → P in BAE32843 (PubMed:16141072).Curated
Sequence conflicti484 – 4841A → T in BAE42242 (PubMed:16141072).Curated
Sequence conflicti484 – 4841A → T in BAE42072 (PubMed:16141072).Curated
Sequence conflicti590 – 5901R → P in AAB18198 (PubMed:9199174).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei168 – 20538Missing in isoform 4. 1 PublicationVSP_017290Add
BLAST
Alternative sequencei347 – 38236Missing in isoform 3. CuratedVSP_017292Add
BLAST
Alternative sequencei402 – 4098WKKHWFVL → TQVPFLRTKIGTF in isoform 5. 1 PublicationVSP_017291
Alternative sequencei1016 – 10249VIEQVSWDN → LKEGLTVQERLKLFESRDLK KD in isoform 2 and isoform 3. 2 PublicationsVSP_017293

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73200 mRNA. Translation: AAB18198.1.
AK154807 mRNA. Translation: BAE32843.1.
AK155630 mRNA. Translation: BAE33355.1.
AK158296 mRNA. Translation: BAE34450.1.
AK162530 mRNA. Translation: BAE36957.1. Different initiation.
AK170849 mRNA. Translation: BAE42072.1.
AK171089 mRNA. Translation: BAE42242.1.
AL596204 Genomic DNA. Translation: CAI24300.1.
AL596204 Genomic DNA. Translation: CAI24303.1.
AL596204 Genomic DNA. Translation: CAI24304.1.
BC049803 mRNA. Translation: AAH49803.1.
BC063067 mRNA. Translation: AAH63067.1.
CCDSiCCDS24774.1. [P97434-2]
CCDS24775.1. [P97434-1]
PIRiT30868.
RefSeqiNP_036157.2. NM_012027.2. [P97434-1]
NP_957697.1. NM_201245.3. [P97434-2]
XP_006533503.1. XM_006533440.1. [P97434-3]
UniGeneiMm.2402.

Genome annotation databases

EnsembliENSMUST00000072031; ENSMUSP00000071914; ENSMUSG00000005417. [P97434-2]
ENSMUST00000108751; ENSMUSP00000104382; ENSMUSG00000005417. [P97434-4]
ENSMUST00000116371; ENSMUSP00000112072; ENSMUSG00000005417. [P97434-1]
GeneIDi26936.
KEGGimmu:26936.
UCSCiuc007jen.2. mouse. [P97434-1]
uc007jeo.2. mouse. [P97434-2]
uc007jep.2. mouse. [P97434-4]
uc011xvo.1. mouse. [P97434-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73200 mRNA. Translation: AAB18198.1.
AK154807 mRNA. Translation: BAE32843.1.
AK155630 mRNA. Translation: BAE33355.1.
AK158296 mRNA. Translation: BAE34450.1.
AK162530 mRNA. Translation: BAE36957.1. Different initiation.
AK170849 mRNA. Translation: BAE42072.1.
AK171089 mRNA. Translation: BAE42242.1.
AL596204 Genomic DNA. Translation: CAI24300.1.
AL596204 Genomic DNA. Translation: CAI24303.1.
AL596204 Genomic DNA. Translation: CAI24304.1.
BC049803 mRNA. Translation: AAH49803.1.
BC063067 mRNA. Translation: AAH63067.1.
CCDSiCCDS24774.1. [P97434-2]
CCDS24775.1. [P97434-1]
PIRiT30868.
RefSeqiNP_036157.2. NM_012027.2. [P97434-1]
NP_957697.1. NM_201245.3. [P97434-2]
XP_006533503.1. XM_006533440.1. [P97434-3]
UniGeneiMm.2402.

3D structure databases

ProteinModelPortaliP97434.
SMRiP97434. Positions 390-481.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205073. 7 interactions.
IntActiP97434. 10 interactions.
MINTiMINT-4132519.
STRINGi10090.ENSMUSP00000071914.

PTM databases

iPTMnetiP97434.
PhosphoSiteiP97434.

Proteomic databases

MaxQBiP97434.
PaxDbiP97434.
PeptideAtlasiP97434.
PRIDEiP97434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072031; ENSMUSP00000071914; ENSMUSG00000005417. [P97434-2]
ENSMUST00000108751; ENSMUSP00000104382; ENSMUSG00000005417. [P97434-4]
ENSMUST00000116371; ENSMUSP00000112072; ENSMUSG00000005417. [P97434-1]
GeneIDi26936.
KEGGimmu:26936.
UCSCiuc007jen.2. mouse. [P97434-1]
uc007jeo.2. mouse. [P97434-2]
uc007jep.2. mouse. [P97434-4]
uc011xvo.1. mouse. [P97434-5]

Organism-specific databases

CTDi23164.
MGIiMGI:1349438. Mprip.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4807. Eukaryota.
ENOG410ZA8F. LUCA.
GeneTreeiENSGT00530000063678.
HOVERGENiHBG023864.
InParanoidiP97434.
TreeFamiTF329258.

Miscellaneous databases

PROiP97434.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005417.
ExpressionAtlasiP97434. baseline and differential.
GenevisibleiP97434. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPRIP_MOUSE
AccessioniPrimary (citable) accession number: P97434
Secondary accession number(s): Q3TBR5
, Q3TC85, Q3TRS0, Q3U1Y8, Q3U3E4, Q5SWZ3, Q5SWZ4, Q5SWZ7, Q6P559, Q80YC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 21, 2006
Last modified: September 7, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.