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Protein

Myosin phosphatase Rho-interacting protein

Gene

Mprip

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Targets myosin phosphatase to the actin cytoskeleton. Required for the regulation of the actin cytoskeleton by RhoA and ROCK1. Depletion leads to an increased number of stress fibers in smooth muscle cells through stabilization of actin fibers by phosphorylated myosin. Overexpression of MRIP as well as its F-actin-binding region leads to disassembly of stress fibers in neuronal cells.2 Publications

GO - Molecular functioni

Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Myosin phosphatase Rho-interacting protein
Alternative name(s):
Rho-interacting protein 3
Short name:
RIP3
p116Rip
Gene namesi
Name:Mprip
Synonyms:Kiaa0864, Mrip, Rhoip3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1349438. Mprip.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi857L → P: Loss of interaction with PPP1R12C and PPP1R12A. 1 Publication1
Mutagenesisi905L → P: No effect. 1 Publication1
Mutagenesisi919I → P: Loss of interaction with PPP1R12C and PPP1R12A. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002238701 – 1024Myosin phosphatase Rho-interacting proteinAdd BLAST1024

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei193PhosphoserineBy similarity1
Modified residuei219PhosphoserineCombined sources1
Modified residuei221PhosphoserineCombined sources1
Modified residuei225PhosphoserineCombined sources1
Modified residuei227PhosphoserineCombined sources1
Modified residuei266PhosphoserineBy similarity1
Modified residuei270PhosphoserineBy similarity1
Modified residuei289PhosphoserineCombined sources1
Modified residuei292PhosphoserineBy similarity1
Modified residuei295PhosphothreonineBy similarity1
Modified residuei364PhosphoserineCombined sources1
Modified residuei492PhosphoserineBy similarity1
Modified residuei617PhosphoserineCombined sources1
Modified residuei645PhosphothreonineBy similarity1
Modified residuei799PhosphoserineBy similarity1
Modified residuei976PhosphoserineCombined sources1
Modified residuei992PhosphoserineBy similarity1
Modified residuei1013PhosphoserineCombined sources1
Modified residuei1015PhosphoserineCombined sources1
Isoform 3 (identifier: P97434-3)
Modified residuei979PhosphoserineCombined sources1
Isoform 2 (identifier: P97434-2)
Modified residuei1015PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP97434.
PaxDbiP97434.
PeptideAtlasiP97434.
PRIDEiP97434.

PTM databases

iPTMnetiP97434.
PhosphoSitePlusiP97434.

Expressioni

Tissue specificityi

Expressed in Kidney, Brain, Heart and Lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000005417.
ExpressionAtlasiP97434. baseline and differential.
GenevisibleiP97434. MM.

Interactioni

Subunit structurei

Binds RHOA, PPP1R12A/MBS and PPP1R12C/MBS85 through adjacent coiled coil domains. Interacts with MYZAP. Binds F-actin through its N-terminus.4 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205073. 7 interactors.
IntActiP97434. 10 interactors.
MINTiMINT-4132519.
STRINGi10090.ENSMUSP00000071914.

Structurei

3D structure databases

ProteinModelPortaliP97434.
SMRiP97434.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini43 – 150PH 1PROSITE-ProRule annotationAdd BLAST108
Domaini386 – 482PH 2PROSITE-ProRule annotationAdd BLAST97

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 382Interaction with F-actinAdd BLAST382
Regioni545 – 823Interaction with RHOA1 PublicationAdd BLAST279
Regioni823 – 878Interaction with PPP1R12ABy similarityAdd BLAST56

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili672 – 976Sequence analysisAdd BLAST305

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi184 – 190Poly-Ser7

Sequence similaritiesi

Contains 2 PH domains.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG4807. Eukaryota.
ENOG410ZA8F. LUCA.
GeneTreeiENSGT00530000063678.
HOVERGENiHBG023864.
InParanoidiP97434.
TreeFamiTF329258.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97434-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSAAKENPCR KFQANIFNKS KCQNCFKPRE SHLLNDEDLT QAKPIYGGWL
60 70 80 90 100
LLAPDGTDFD NPVHRSRKWQ RRFFILYEHG LLRYALDEMP TTLPQGTINM
110 120 130 140 150
NQCTDVVDGE ARTGQKFSLC ILTPDKEHFI RAETKEIISG WLEMLMVYPR
160 170 180 190 200
TNKQNQKKKR KVEPPTPQEP GPAKMAVTSS SGGSSGSSSS IPSAEKVPTT
210 220 230 240 250
KSTLWQEEMR AKDQPDGTSL SPAQSPSQSQ PPAACTPREP GLESKEDEST
260 270 280 290 300
ISGDRVDGGR KVRVESGYFS LEKAKQDLRA EEQLPPLLSP PSPSTPHSRR
310 320 330 340 350
SQVIEKFEAL DIEKAEHMET NMLILTTPSS DTRQGRSERR AIPRKRDFAS
360 370 380 390 400
EAPTAPLSDA CPLSPHRRAK SLDRRSTESS MTPDLLNFKK GWLTKQYEDG
410 420 430 440 450
QWKKHWFVLA DQSLRYYRDS VAEEAADLDG EINLSTCYDV TEYPVQRNYG
460 470 480 490 500
FQIHTKEGEF TLSAMTSGIR RNWIQTIMKH VLPASAPDVT SSLPEGKNKS
510 520 530 540 550
TSFETCSRST EKQEAEPGEP DPEQKKSRAR ERRREGRSKT FDWAEFRPIQ
560 570 580 590 600
QALAQERASA VGSSDSGDPG CLEAEPGELE RERARRREER RKRFGMLDTI
610 620 630 640 650
DGPGMEDTAL RMDIDRSPGL LGTPDLKTQN VHVEIEQRWH QVETTPLREE
660 670 680 690 700
KQVPIAPLHL SLEDRSERLS THELTSLLEK ELEQSQKEAS DLLEQNRLLQ
710 720 730 740 750
DQLRVALGRE QSAREGYVLQ ATCERGFAAM EETHQKKIED LQRQHQRELE
760 770 780 790 800
KLREEKDRLL AEETAATISA IEAMKNAHRE EMERELEKSQ RSQISSINSD
810 820 830 840 850
IEALRRQYLE ELQSVQRELE VLSEQYSQKC LENAHLAQAL EAERQALRQC
860 870 880 890 900
QRENQELNAH NQELNNRLAA EITRLRTLLT GDGGGESTGL PLTQGKDAYE
910 920 930 940 950
LEVLLRVKES EIQYLKQEIS SLKDELQTAL RDKKYASDKY KDIYTELSIA
960 970 980 990 1000
KAKADCDISR LKEQLKAATE ALGEKSPEGT TVSGYDIMKS KSNPDFLKKD
1010 1020
RSCVTRQLRN IRSKSVIEQV SWDN
Length:1,024
Mass (Da):116,408
Last modified:February 21, 2006 - v2
Checksum:i7BE3BFB89011ECC5
GO
Isoform 2 (identifier: P97434-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1016-1024: VIEQVSWDN → LKEGLTVQERLKLFESRDLKKD

Show »
Length:1,037
Mass (Da):117,965
Checksum:i44AD4103995E08F7
GO
Isoform 3 (identifier: P97434-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     347-382: Missing.
     1016-1024: VIEQVSWDN → LKEGLTVQERLKLFESRDLKKD

Note: Gene prediction based on human cDNA data. No experimental confirmation available.Combined sources
Show »
Length:1,001
Mass (Da):114,094
Checksum:iC718480566C25CA9
GO
Isoform 4 (identifier: P97434-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     168-205: Missing.

Note: No experimental confirmation available.
Show »
Length:986
Mass (Da):112,703
Checksum:iF8F9FF6AA58B6330
GO
Isoform 5 (identifier: P97434-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     402-409: WKKHWFVL → TQVPFLRTKIGTF

Note: No experimental confirmation available.
Show »
Length:1,029
Mass (Da):116,773
Checksum:iB14325EC3B88E5A5
GO

Sequence cautioni

The sequence BAE36957 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti43K → E in BAE32843 (PubMed:16141072).Curated1
Sequence conflicti70Q → R in BAE33355 (PubMed:16141072).Curated1
Sequence conflicti184S → T in AAB18198 (PubMed:9199174).Curated1
Sequence conflicti289S → P in BAE42242 (PubMed:16141072).Curated1
Sequence conflicti301S → P in BAE32843 (PubMed:16141072).Curated1
Sequence conflicti484A → T in BAE42242 (PubMed:16141072).Curated1
Sequence conflicti484A → T in BAE42072 (PubMed:16141072).Curated1
Sequence conflicti590R → P in AAB18198 (PubMed:9199174).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_017290168 – 205Missing in isoform 4. 1 PublicationAdd BLAST38
Alternative sequenceiVSP_017292347 – 382Missing in isoform 3. CuratedAdd BLAST36
Alternative sequenceiVSP_017291402 – 409WKKHWFVL → TQVPFLRTKIGTF in isoform 5. 1 Publication8
Alternative sequenceiVSP_0172931016 – 1024VIEQVSWDN → LKEGLTVQERLKLFESRDLK KD in isoform 2 and isoform 3. 2 Publications9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73200 mRNA. Translation: AAB18198.1.
AK154807 mRNA. Translation: BAE32843.1.
AK155630 mRNA. Translation: BAE33355.1.
AK158296 mRNA. Translation: BAE34450.1.
AK162530 mRNA. Translation: BAE36957.1. Different initiation.
AK170849 mRNA. Translation: BAE42072.1.
AK171089 mRNA. Translation: BAE42242.1.
AL596204 Genomic DNA. Translation: CAI24300.1.
AL596204 Genomic DNA. Translation: CAI24303.1.
AL596204 Genomic DNA. Translation: CAI24304.1.
BC049803 mRNA. Translation: AAH49803.1.
BC063067 mRNA. Translation: AAH63067.1.
CCDSiCCDS24774.1. [P97434-2]
CCDS24775.1. [P97434-1]
PIRiT30868.
RefSeqiNP_036157.2. NM_012027.2. [P97434-1]
NP_957697.1. NM_201245.3. [P97434-2]
XP_006533503.1. XM_006533440.1. [P97434-3]
UniGeneiMm.2402.

Genome annotation databases

EnsembliENSMUST00000072031; ENSMUSP00000071914; ENSMUSG00000005417. [P97434-2]
ENSMUST00000108751; ENSMUSP00000104382; ENSMUSG00000005417. [P97434-4]
ENSMUST00000116371; ENSMUSP00000112072; ENSMUSG00000005417. [P97434-1]
GeneIDi26936.
KEGGimmu:26936.
UCSCiuc007jen.2. mouse. [P97434-1]
uc007jeo.2. mouse. [P97434-2]
uc007jep.2. mouse. [P97434-4]
uc011xvo.1. mouse. [P97434-5]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73200 mRNA. Translation: AAB18198.1.
AK154807 mRNA. Translation: BAE32843.1.
AK155630 mRNA. Translation: BAE33355.1.
AK158296 mRNA. Translation: BAE34450.1.
AK162530 mRNA. Translation: BAE36957.1. Different initiation.
AK170849 mRNA. Translation: BAE42072.1.
AK171089 mRNA. Translation: BAE42242.1.
AL596204 Genomic DNA. Translation: CAI24300.1.
AL596204 Genomic DNA. Translation: CAI24303.1.
AL596204 Genomic DNA. Translation: CAI24304.1.
BC049803 mRNA. Translation: AAH49803.1.
BC063067 mRNA. Translation: AAH63067.1.
CCDSiCCDS24774.1. [P97434-2]
CCDS24775.1. [P97434-1]
PIRiT30868.
RefSeqiNP_036157.2. NM_012027.2. [P97434-1]
NP_957697.1. NM_201245.3. [P97434-2]
XP_006533503.1. XM_006533440.1. [P97434-3]
UniGeneiMm.2402.

3D structure databases

ProteinModelPortaliP97434.
SMRiP97434.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205073. 7 interactors.
IntActiP97434. 10 interactors.
MINTiMINT-4132519.
STRINGi10090.ENSMUSP00000071914.

PTM databases

iPTMnetiP97434.
PhosphoSitePlusiP97434.

Proteomic databases

MaxQBiP97434.
PaxDbiP97434.
PeptideAtlasiP97434.
PRIDEiP97434.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000072031; ENSMUSP00000071914; ENSMUSG00000005417. [P97434-2]
ENSMUST00000108751; ENSMUSP00000104382; ENSMUSG00000005417. [P97434-4]
ENSMUST00000116371; ENSMUSP00000112072; ENSMUSG00000005417. [P97434-1]
GeneIDi26936.
KEGGimmu:26936.
UCSCiuc007jen.2. mouse. [P97434-1]
uc007jeo.2. mouse. [P97434-2]
uc007jep.2. mouse. [P97434-4]
uc011xvo.1. mouse. [P97434-5]

Organism-specific databases

CTDi23164.
MGIiMGI:1349438. Mprip.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG4807. Eukaryota.
ENOG410ZA8F. LUCA.
GeneTreeiENSGT00530000063678.
HOVERGENiHBG023864.
InParanoidiP97434.
TreeFamiTF329258.

Miscellaneous databases

PROiP97434.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005417.
ExpressionAtlasiP97434. baseline and differential.
GenevisibleiP97434. MM.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00169. PH. 2 hits.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiPS50003. PH_DOMAIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMPRIP_MOUSE
AccessioniPrimary (citable) accession number: P97434
Secondary accession number(s): Q3TBR5
, Q3TC85, Q3TRS0, Q3U1Y8, Q3U3E4, Q5SWZ3, Q5SWZ4, Q5SWZ7, Q6P559, Q80YC8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: February 21, 2006
Last modified: November 2, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.