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Protein

Rho guanine nucleotide exchange factor 28

Gene

Arhgef28

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as a RHOA-specific guanine nucleotide exchange factor regulating signaling pathways downstream of integrins and growth factor receptors. Functions in axonal branching, synapse formation and dendritic morphogenesis. Functions also in focal adhesion formation, cell motility and B-lymphocytes activation. May regulate NEFL expression and aggregation and play a role in apoptosis.10 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri651 – 69848Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • central nervous system neuron axonogenesis Source: MGI
  • intracellular signal transduction Source: InterPro
  • neurofilament cytoskeleton organization Source: MGI
  • regulation of Rho protein signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Differentiation

Keywords - Ligandi

Metal-binding, RNA-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Rho guanine nucleotide exchange factor 28
Alternative name(s):
190 kDa guanine nucleotide exchange factor
Short name:
p190-RhoGEF
Short name:
p190RhoGEF
Rho guanine nucleotide exchange factor
Rho-interacting protein 2
Gene namesi
Name:Arhgef28
Synonyms:Kiaa1998, Rgnef, Rhoip2, Rip2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1346016. Arhgef28.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi1003 – 10031Y → A: Loss of function. 1 Publication
Mutagenesisi1375 – 13751N → A: Alters interaction with YWHAH. 1 Publication
Mutagenesisi1376 – 13761L → A: Alters interaction with YWHAH. 1 Publication
Mutagenesisi1563 – 15631F → A: Alters interaction with MAPK8IP1. 1 Publication
Mutagenesisi1565 – 15651N → A: Alters interaction with MAPK8IP1. 1 Publication
Mutagenesisi1568 – 15681F → A: Alters interaction with MAPK8IP1. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 16931693Rho guanine nucleotide exchange factor 28PRO_0000080967Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei312 – 3121PhosphoserineBy similarity
Modified residuei314 – 3141PhosphoserineBy similarity
Modified residuei478 – 4781PhosphoserineBy similarity
Modified residuei623 – 6231PhosphoserineBy similarity
Modified residuei1535 – 15351PhosphoserineBy similarity
Modified residuei1604 – 16041PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated on tyrosine upon stimulation of cells by laminin.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP97433.
PaxDbiP97433.
PRIDEiP97433.

PTM databases

iPTMnetiP97433.
PhosphoSiteiP97433.

Expressioni

Tissue specificityi

Highly enriched in the brain (at protein level). Also detected in lung and kidney.2 Publications

Inductioni

Up-regulated in B-lymphocytes upon CD40 stimulation. Up-regulated by PTK2B/PYK2 (at protein level).2 Publications

Gene expression databases

CleanExiMM_RGNEF.

Interactioni

Subunit structurei

Homooligomer; forms some cytoplasmic aggregates. Forms a complex with MAPK8 and MAPK8IP1. Interacts with RHOA. Interacts with microtubules. Interacts with YWHAE and YWHAH. Interacts with PTK2/FAK1. Interacts with NEFL. Interacts with CTNND2; prevents interaction with RHOA.7 Publications

Protein-protein interaction databases

IntActiP97433. 2 interactions.
MINTiMINT-111716.
STRINGi10090.ENSMUSP00000105053.

Structurei

3D structure databases

ProteinModelPortaliP97433.
SMRiP97433. Positions 818-1186.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini846 – 1041196DHPROSITE-ProRule annotationAdd
BLAST
Domaini1095 – 118490PHPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1292 – 130110Interaction with PTK2/FAK1; required for regulation of axonal branching and synapse formation
Regioni1369 – 138012Mediates cytoplasmic retention and interaction with YWHAHAdd
BLAST
Regioni1421 – 1693273Interaction with microtubulesAdd
BLAST
Regioni1493 – 152432RNA-bindingBy similarityAdd
BLAST
Regioni1563 – 157614Mediates cytoplasmic retention and interaction with MAPK8IP1Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili1421 – 1522102Sequence analysisAdd
BLAST

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri651 – 69848Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG3519. Eukaryota.
KOG3520. Eukaryota.
ENOG410XT68. LUCA.
HOGENOMiHOG000154109.
HOVERGENiHBG079129.
InParanoidiP97433.
PhylomeDBiP97433.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97433-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MELSCSEVPL YGQKTVYAKF GKNVYLPEDA EFYFVYGGSH QRHVVIADRV
60 70 80 90 100
QDNVLQSSIP GHWLQETVTV SVCLCSEGYS PVTMGSGSVT YVDNMACRLA
110 120 130 140 150
RLLVTQADRL TACSHQTLLT PFALTVEALP ALDEELVLAL TQLELPLGWT
160 170 180 190 200
VLGNSSLEVS LHRESLLHLA VRWALPKLFH FLLCLPGGVK ALKLPNEEAT
210 220 230 240 250
TPLDLALQGG HSTLVEDITN FQGSHSPGFS RLRLNEEATL QFVHSSETLT
260 270 280 290 300
LTVNHTAEHL LEADIKLFRK YFWDRAFLVK ALEQEAKTEK ATMPSGAAET
310 320 330 340 350
EEEVRNLESG RSPSEEEEDA KSIKSQVDGP SEHEDQDRLA LDRSFDGLKK
360 370 380 390 400
SKHVPASLAA GQLSDVLNGG DEVYANCMVI DQVGDLDINY INLEGLSTHT
410 420 430 440 450
SPESGRSMLG PQACMHTLPP DTSPCGRPLI ENSEGTLDAA ASQSFVTPSS
460 470 480 490 500
SRTSNLNLSF GLHGFEKEQS HLKKRSSSLD ALVADSEGEG GSEPPICYAV
510 520 530 540 550
GSQSSPRTGL PSGDELDSFE TNTEPDCNIS RTESLSLSST LHSKESLLSG
560 570 580 590 600
IRSRSYSCSS PKISSGKSRL VRDFTVCSTS EEQRSYSFQE PPGEKRIQEE
610 620 630 640 650
EWDEYVIPAK SESEKYKVSR TFSFLMNRMT SPRNKSKMKN KDTKEKEKMN
660 670 680 690 700
RHQFVPGTFS GVLQCSGCDK TLLGKESLQC ANCKANTHKG CKDAVPPCTK
710 720 730 740 750
KFQEKYNKNK PQSILGSSSV RDVPAPGLSL HPSSSMPIGL PAGRKEFAAQ
760 770 780 790 800
VHPLSRSVPG TTLESFRRAV TSLESEGDSW RSRSHSDELF QSMGSSPSTE
810 820 830 840 850
SFMMEDVVDS SLWIDLSSDA QEFEAESWSL VVDPSFCSRQ EKDVIKRQDV
860 870 880 890 900
IFELMQTEVH HIQTLLIMSE VFRKGMKEEL QLDHSTVDKI FPCLDELLET
910 920 930 940 950
HRHFFFSMKE RRQESCAGSD RNFVINQIGD ILVQQFSEEN ASKMKRIYGE
960 970 980 990 1000
FCSHHKEAMS LFKELQQNKK FQNFIKIRNS NLLARRRGIP ECILLVTQRI
1010 1020 1030 1040 1050
TKYPVLVERI LQYTKERTEE HRDLCKALGL IKDMIAAVDL KVSEYEKNQK
1060 1070 1080 1090 1100
WLEILNKIEN KTYTKLKNGH VFRKQALLSQ ERALLHDGLV YWKTATGRFK
1110 1120 1130 1140 1150
DILALLLTDV LLFLQEKDQK YIFAAVDQKP SVISLQKLIA REVANEERGM
1160 1170 1180 1190 1200
FLISASSAGP EMYEIHTNSK EERNNWMRRI QQAVESCPEE EGGRTSESDE
1210 1220 1230 1240 1250
ERRKAEARVA KIQQCQEILS NQDQQICTYL EEKLHIYAEL GELSGFEDVH
1260 1270 1280 1290 1300
LEPHLLIKPD PGEPPQAASL LAAALREAES LQVAVKASKM GDVSQSSEES
1310 1320 1330 1340 1350
PGGTVLMDTP STQDVPASPT ASLVTEGTEG RGCWDVDPGL QGVVTDLAVS
1360 1370 1380 1390 1400
DAGEKVEYRS FSGSSQSEII QAIQNLTRLL YSLQAALTIQ DSHIEIHKLV
1410 1420 1430 1440 1450
LQQRESLAPS HSFRGGPLQD QEKSRYLEKQ REELANIHKL QHQFQQEQRR
1460 1470 1480 1490 1500
WHRTCDQQQR EQEAQESWLQ ARERECQSQE ELLLRHRSEL DHQLQEYQQS
1510 1520 1530 1540 1550
LERLREGQRM VERERQKMRV QQGLLGHCKH SRQRSLPAVF SPGSKEVTEL
1560 1570 1580 1590 1600
NRAESLCHEN SFFINEAFGH MSLNTSNKPN PSGVPWDAHP LEGSHFDLAR
1610 1620 1630 1640 1650
TSESPTELKI DISQPPDVNS ELWTTGPGHQ RPALQENSKE SYKNVADLDS
1660 1670 1680 1690
FQSESSSPQD SNQRWPPATD THNRSKTKSS DGGWTRWRCW RRG
Length:1,693
Mass (Da):190,326
Last modified:May 1, 1997 - v1
Checksum:i4E96087C449FF14C
GO
Isoform 2 (identifier: P97433-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1322-1324: SLV → CGI
     1325-1693: Missing.

Show »
Length:1,324
Mass (Da):147,757
Checksum:iB86E628B11ED16A3
GO

Sequence cautioni

The sequence AAH22978 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH75734 differs from that shown.Contaminating sequence. Potential poly-A sequence.Curated
The sequence BAD32603 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti340 – 3401A → P in AAH75734 (PubMed:15489334).Curated
Sequence conflicti481 – 4811A → T in AAH75734 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1322 – 13243SLV → CGI in isoform 2. 1 PublicationVSP_032145
Alternative sequencei1325 – 1693369Missing in isoform 2. 1 PublicationVSP_032146Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73199 mRNA. Translation: AAB18197.1.
AK173325 mRNA. Translation: BAD32603.1. Different initiation.
BC022978 mRNA. Translation: AAH22978.1. Sequence problems.
BC075734 mRNA. Translation: AAH75734.1. Sequence problems.
PIRiT30867.
RefSeqiNP_036156.2. NM_012026.2.
UniGeneiMm.252718.

Genome annotation databases

GeneIDi110596.
KEGGimmu:110596.
UCSCiuc007rol.1. mouse. [P97433-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U73199 mRNA. Translation: AAB18197.1.
AK173325 mRNA. Translation: BAD32603.1. Different initiation.
BC022978 mRNA. Translation: AAH22978.1. Sequence problems.
BC075734 mRNA. Translation: AAH75734.1. Sequence problems.
PIRiT30867.
RefSeqiNP_036156.2. NM_012026.2.
UniGeneiMm.252718.

3D structure databases

ProteinModelPortaliP97433.
SMRiP97433. Positions 818-1186.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP97433. 2 interactions.
MINTiMINT-111716.
STRINGi10090.ENSMUSP00000105053.

PTM databases

iPTMnetiP97433.
PhosphoSiteiP97433.

Proteomic databases

MaxQBiP97433.
PaxDbiP97433.
PRIDEiP97433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi110596.
KEGGimmu:110596.
UCSCiuc007rol.1. mouse. [P97433-2]

Organism-specific databases

CTDi64283.
MGIiMGI:1346016. Arhgef28.
RougeiSearch...

Phylogenomic databases

eggNOGiKOG3519. Eukaryota.
KOG3520. Eukaryota.
ENOG410XT68. LUCA.
HOGENOMiHOG000154109.
HOVERGENiHBG079129.
InParanoidiP97433.
PhylomeDBiP97433.

Miscellaneous databases

PROiP97433.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RGNEF.

Family and domain databases

Gene3Di1.20.900.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR000219. DH-domain.
IPR002219. PE/DAG-bd.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00233. PH. 1 hit.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50010. DH_2. 1 hit.
PS50003. PH_DOMAIN. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiARG28_MOUSE
AccessioniPrimary (citable) accession number: P97433
Secondary accession number(s): A0PJC1, Q69Z41, Q6DI55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.