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P97432 (NBR1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Next to BRCA1 gene 1 protein
Alternative name(s):
Membrane component chromosome 17 surface marker 2 homolog
Neighbor of BRCA1 gene 1 protein
Gene names
Name:Nbr1
Synonyms:M17s2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length988 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts probably as a receptor for selective autophagosomal degradation of ubiquitinated targets By similarity.

Subunit structure

Homooligomer and heterooligomer By similarity. Interacts with SQSTM1, titin/TTN, TRIM55, RNF29, USP8, SQSTM1, MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2 By similarity. Binds to ubiquitin and ubiquitinated proteins By similarity.

Subcellular location

Cytoplasm By similarity. Cytoplasmic vesicle › autophagosome By similarity. Lysosome By similarity. Cytoplasm › myofibril › sarcomere › M line By similarity. Note: In cardiac muscles localizes to the sarcomeric M line By similarity. Is targeted to lysosomes for degradation By similarity.

Tissue specificity

Expressed in brain.

Domain

The OPR domain mediates interaction with SQSTM1 By similarity.

Sequence similarities

Contains 1 OPR domain.

Contains 1 UBA domain.

Contains 1 ZZ-type zinc finger.

Ontologies

Keywords
   Cellular componentCytoplasm
Cytoplasmic vesicle
Lysosome
   DomainZinc-finger
   LigandMetal-binding
Zinc
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmacroautophagy

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of osteoblast differentiation

Inferred from mutant phenotype PubMed 20616007. Source: BHF-UCL

protein oligomerization

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of bone mineralization

Inferred from mutant phenotype PubMed 20616007. Source: BHF-UCL

regulation of stress-activated MAPK cascade

Inferred from mutant phenotype PubMed 20616007. Source: BHF-UCL

   Cellular_componentM band

Inferred from electronic annotation. Source: UniProtKB-SubCell

autophagic vacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

cytoplasmic vesicle

Inferred from electronic annotation. Source: UniProtKB-KW

cytosol

Inferred from sequence or structural similarity. Source: UniProtKB

late endosome

Inferred from direct assay PubMed 19250911. Source: UniProtKB

lysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

pre-autophagosomal structure

Inferred from direct assay PubMed 21220506. Source: MGI

   Molecular_functionmitogen-activated protein kinase binding

Inferred from direct assay PubMed 20616007. Source: BHF-UCL

ubiquitin binding

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

MAP1LC3BQ9GZQ82EBI-642554,EBI-373144From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 988988Next to BRCA1 gene 1 protein
PRO_0000096747

Regions

Domain43 – 7230OPR
Domain935 – 97945UBA
Zinc finger212 – 25847ZZ-type
Region543 – 63795ATG8 family proteins-binding By similarity
Region745 – 75612ATG8 family proteins-binding By similarity
Compositional bias729 – 7335Poly-Glu

Experimental info

Sequence conflict8071Q → R in AAF74118. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P97432 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: E5BB4B281FE33CE0

FASTA988109,958
        10         20         30         40         50         60 
MEPQVTLNVT FKNETQSFLV SDPENTTWAD VEAMVKVSFD LNTIQIKYLD EENEEISINS 

        70         80         90        100        110        120 
QGEYEEALKM ANIKQGNQLQ MQVHEGYHVV DEALPKNVVE NQAAARTGKK PLAHYSSLVR 

       130        140        150        160        170        180 
VLGSDMKTTE EPAPEQCSSA PCDTDQPQDK PPDWFTSYLE MFREQVVKET VEKLEQRLQE 

       190        200        210        220        230        240 
KLVLQKPLLS SSPTEVSMPI SEETLFLPEN QFSWHIACSH CQKRIVGVRY QCSLCPSYNI 

       250        260        270        280        290        300 
CEDCEAGPYT HDTNHVLLKL RRPVVISSEP FFYSKYSAPR LPAALEQVRL QKQVDKNFVK 

       310        320        330        340        350        360 
AEKQRLRAEK KQRKAEVKEL KKQLKLHRKI HLWNSIHGLQ SPKSPLGRPE SLLQSNTLML 

       370        380        390        400        410        420 
PLQPCAPVMP TLSAAFVDEN LPDGTHLQPG TKFIKHWRMK NTGNVKWNTD TKLKFMWGNL 

       430        440        450        460        470        480 
TLASTEKKDV LVPCLKAGHV GVVSVEFIAP TLEGTYTSHW RLSHKGQQFG PRVWCSIIVD 

       490        500        510        520        530        540 
PFPSSESPDN VEGDRISSSK ADDFSCEQEE AFLLAEEEIP LGEVTKQTEG TGASASQKTR 

       550        560        570        580        590        600 
RAASERELYI PSVDLLTAQD LLSFELLDIN IVQELERVPH NTPVDMTPCM SPLPHDSPLI 

       610        620        630        640        650        660 
EKPGLGQIQE ESEGAGFKAP PDSTVSAKRK AETPASVEET EEDLSGTQFV CETVIRSLTL 

       670        680        690        700        710        720 
DAAPDHNPPC RQRSPQRELQ LYSTEGQQPL VLPGFCRKDS SLKFALPEEG PRGDEREEIV 

       730        740        750        760        770        780 
HIVEEEVVEE EEEVQDEEVQ SQSSASSEDY IIILPECFDT SRPLGDSMYS SALSQPGLER 

       790        800        810        820        830        840 
GAEGEPGIES GLEPTEARER LPERESQPQE QSISDILTTS QPLDTVPLVP EVAGLPAALS 

       850        860        870        880        890        900 
RSAPCGQCES SGVDSPGVDS PATMHEVPPA PDDIRGEPRG STGLANSRQR SCDHSRHHNG 

       910        920        930        940        950        960 
SSIAGGLVKG ALSVAASAYK ALFSGPPVTA QPIVSEDQTT ALMAHLFEMG FCDRQLNLRL 

       970        980 
LRKHNYNILQ VVTELLQVNN NDWYSHRY

« Hide

References

[1]"Isolation of the murine Nbr1 gene adjacent to the murine Brca1 gene."
Chambers J.A., Solomon E.
Genomics 38:305-313(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: ICR.
Tissue: Brain.
[2]"Expression profiles and intergenic structure of head-to-head oriented Brca1 and Nbr1 genes."
Dimitrov S., Brennerova M., Forejt J.
Gene 262:89-98(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129/Sv.
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U73039 mRNA. Translation: AAC53025.1.
AF227188 mRNA. Translation: AAF74118.1.
IPIIPI00125341.
RefSeqNP_001239149.1. NM_001252220.1.
NP_032702.1. NM_008676.3.
UniGeneMm.489817.
Mm.784.

3D structure databases

ProteinModelPortalP97432.
SMRP97432. Positions 1-86, 935-979.
ModBaseSearch...

Protein-protein interaction databases

IntActP97432. 7 interactions.
MINTMINT-1591819.

PTM databases

PhosphoSiteP97432.

Proteomic databases

PaxDbP97432.
PRIDEP97432.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103098; ENSMUSP00000099387; ENSMUSG00000017119.
ENSMUST00000103099; ENSMUSP00000099388; ENSMUSG00000017119.
ENSMUST00000107218; ENSMUSP00000102836; ENSMUSG00000017119.
GeneID17966.
KEGGmmu:17966.

Organism-specific databases

CTD4077.
MGIMGI:108498. Nbr1.

Phylogenomic databases

eggNOGNOG239857.
GeneTreeENSGT00390000016335.
HOGENOMHOG000220861.
HOVERGENHBG052578.
InParanoidP97432.
OMAPDHNPPC.
OrthoDBEOG4Z62MX.

Gene expression databases

ArrayExpressP97432.
BgeeP97432.
CleanExMM_NBR1.
GenevestigatorP97432.
GermOnlineENSMUSG00000017119. Mus musculus.

Family and domain databases

InterProIPR000270. OPR_PB1.
IPR009060. UBA-like.
IPR015940. UBA/transl_elong_EF1B_N_euk.
IPR000433. Znf_ZZ.
[Graphical view]
PfamPF00564. PB1. 1 hit.
PF00569. ZZ. 1 hit.
[Graphical view]
SMARTSM00666. PB1. 1 hit.
SM00291. ZnF_ZZ. 1 hit.
[Graphical view]
SUPFAMSSF46934. UBA_like. 1 hit.
PROSITEPS50030. UBA. 1 hit.
PS01357. ZF_ZZ_1. 1 hit.
PS50135. ZF_ZZ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNBR1. mouse.
NextBio292899.
SOURCESearch...

Entry information

Entry nameNBR1_MOUSE
AccessionPrimary (citable) accession number: P97432
Secondary accession number(s): Q9JIH9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: May 1, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents