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P97431 (IRF6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interferon regulatory factor 6

Short name=IRF-6
Gene names
Name:Irf6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length467 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development. Plays a role in regulating mammary epithelial cell proliferation. May regulate WDR65 transcription. Ref.7 Ref.8 Ref.9 Ref.10

Subunit structure

Interacts with SERPINB5 By similarity. Ref.9

Subcellular location

Nucleus Potential. Cytoplasm. Note: Translocates to nucleus in response to an activating signal Probable. Ref.9

Tissue specificity

High levels of expression along the medial edge of the fusing palate, tooth buds, hair follicles, genitalia and skin. Ref.6

Post-translational modification

Phosphorylated By similarity. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation By similarity.

Sequence similarities

Belongs to the IRF family.

Contains 1 IRF tryptophan pentad repeat DNA-binding domain.

Ontologies

Keywords
   Biological processDifferentiation
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   LigandDNA-binding
   PTMUbl conjugation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell cycle arrest

Inferred from electronic annotation. Source: Ensembl

cell development

Inferred from mutant phenotype Ref.7. Source: MGI

keratinocyte differentiation

Inferred from mutant phenotype PubMed 17041601Ref.7. Source: MGI

keratinocyte proliferation

Inferred from mutant phenotype PubMed 17041601Ref.7. Source: MGI

mammary gland epithelial cell differentiation

Inferred from expression pattern Ref.8. Source: UniProtKB

negative regulation of cell proliferation

Inferred from genetic interaction Ref.7. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from direct assay Ref.9. Source: UniProtKB

skin development

Inferred from mutant phenotype PubMed 17041601Ref.7. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionDNA binding

Inferred from direct assay Ref.9. Source: UniProtKB

regulatory region DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.9. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 467467Interferon regulatory factor 6
PRO_0000154561

Regions

DNA binding7 – 115109IRF tryptophan pentad repeat Ref.9

Experimental info

Mutagenesis841R → C: Mice show an atypical limb development, are covered by an abnormal smooth skin and die shortly after birth. The esophageal lumen is obliterated by adhesion of the stratified, squamous epithelium lining this structure. The hindlimbs, tail and body are fused together by a thickened epidermis. Epidermis is hyperproliferative but does not undergo a normal differentiation program. Ref.7
Sequence conflict1191T → P in AAB36714. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P97431 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 1C564BC8D79C5259

FASTA46753,111
        10         20         30         40         50         60 
MALHPRRVRL KPWLVAQVDS GLYPGLIWLH RDSKRFQIPW KHATRHSPQQ EEENTIFKAW 

        70         80         90        100        110        120 
AVETGKYQEG VDDPDPAKWK AQLRCALNKS REFNLMYDGT KEVPMNPVKI YQVCDIPQTQ 

       130        140        150        160        170        180 
GSVINPGSTG SAPWDEKDND VDEDEEEDEL EQSQHHVPIQ DTFPFLNING SPMAPASVGN 

       190        200        210        220        230        240 
CSVGNCSPES VWPKTEPLEM EVPQAPIQPF YSSPELWISS LPMTDLDIKF QYRGKEYGQT 

       250        260        270        280        290        300 
MTVSNPQGCR LFYGDLGPMP DQEELFGPVS LEQVKFPGPE HITNEKQKLF TSKLLDVMDR 

       310        320        330        340        350        360 
GLILEVSGHA IYAIRLCQCK VYWSGPCAPS LAAPNLIERQ KKVKLFCLET FLSELIAHQK 

       370        380        390        400        410        420 
GQIEKQPPFE IYLCFGEEWP DGKPLERKLI LVQVIPVVAR MIYEMFSGDF TRSFDSGSVR 

       430        440        450        460 
LQISTPDIKD NIVAQLKQLY RILQTQESWQ PMQPAPSMQL PQALPAQ 

« Hide

References

« Hide 'large scale' references
[1]Grossman A., Mittrucker H.W., Antonio L., Mak T.W.
Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Colon.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Kidney and Oviduct.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[6]"Mutations in IRF6 cause Van der Woude and popliteal pterygium syndromes."
Kondo S., Schutte B.C., Richardson R.J., Bjork B.C., Knight A.S., Watanabe Y., Howard E., de Lima R.L.L., Daack-Hirsch S., Sander A., McDonald-McGinn D.M., Zackai E.H., Lammer E.J., Aylsworth A.S., Ardinger H.H., Lidral A.C., Pober B.R., Moreno L. expand/collapse author list , Arcos-Burgos M., Valencia C., Houdayer C., Bahuau M., Moretti-Ferreira D., Richieri-Costa A., Dixon M.J., Murray J.C.
Nat. Genet. 32:285-289(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Irf6 is a key determinant of the keratinocyte proliferation-differentiation switch."
Richardson R.J., Dixon J., Malhotra S., Hardman M.J., Knowles L., Boot-Handford R.P., Shore P., Whitmarsh A., Dixon M.J.
Nat. Genet. 38:1329-1334(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF ARG-84.
[8]"Interferon regulatory factor 6 promotes cell cycle arrest and is regulated by the proteasome in a cell cycle-dependent manner."
Bailey C.M., Abbott D.E., Margaryan N.V., Khalkhali-Ellis Z., Hendrix M.J.C.
Mol. Cell. Biol. 28:2235-2243(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"Missense mutations that cause Van der Woude syndrome and popliteal pterygium syndrome affect the DNA-binding and transcriptional activation functions of IRF6."
Little H.J., Rorick N.K., Su L.-I., Baldock C., Malhotra S., Jowitt T., Gakhar L., Subramanian R., Schutte B.C., Dixon M.J., Shore P.
Hum. Mol. Genet. 18:535-545(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING, SUBUNIT, SUBCELLULAR LOCATION.
[10]"Genomic strategy identifies a missense mutation in WD-repeat domain 65 (WDR65) in an individual with Van der Woude syndrome."
Rorick N.K., Kinoshita A., Weirather J.L., Peyrard-Janvid M., de Lima R.L., Dunnwald M., Shanske A.L., Moretti-Ferreira D., Koillinen H., Kere J., Mansilla M.A., Murray J.C., Goudy S.L., Schutte B.C.
Am. J. Med. Genet. A 155:1314-1321(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U73029 mRNA. Translation: AAB36714.1.
AK143299 mRNA. Translation: BAE25338.1.
AK143954 mRNA. Translation: BAE25628.1.
AL365322 Genomic DNA. Translation: CAX15615.1.
CH466555 Genomic DNA. Translation: EDL12956.1.
BC008515 mRNA. Translation: AAH08515.1.
CCDSCCDS15632.1.
RefSeqNP_058547.2. NM_016851.2.
XP_006497325.1. XM_006497262.1.
XP_006497326.1. XM_006497263.1.
UniGeneMm.273695.

3D structure databases

ProteinModelPortalP97431.
SMRP97431. Positions 12-112, 214-445.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000075839.

PTM databases

PhosphoSiteP97431.

Proteomic databases

MaxQBP97431.
PaxDbP97431.
PRIDEP97431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000076521; ENSMUSP00000075839; ENSMUSG00000026638.
GeneID54139.
KEGGmmu:54139.
UCSCuc007edy.1. mouse.

Organism-specific databases

CTD3664.
MGIMGI:1859211. Irf6.

Phylogenomic databases

eggNOGNOG45968.
GeneTreeENSGT00740000115254.
HOGENOMHOG000037433.
HOVERGENHBG105715.
InParanoidQ91VD0.
KOK10154.
OMAHEEENTI.
OrthoDBEOG7CCBR1.
TreeFamTF328512.

Gene expression databases

BgeeP97431.
CleanExMM_IRF6.
GenevestigatorP97431.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.60.200.10. 1 hit.
InterProIPR019817. Interferon_reg_fac_CS.
IPR001346. Interferon_reg_fact_DNA-bd_dom.
IPR019471. Interferon_reg_factor-3.
IPR017855. SMAD_dom-like.
IPR008984. SMAD_FHA_domain.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF00605. IRF. 1 hit.
PF10401. IRF-3. 1 hit.
[Graphical view]
PRINTSPR00267. INTFRNREGFCT.
SMARTSM00348. IRF. 1 hit.
[Graphical view]
SUPFAMSSF49879. SSF49879. 1 hit.
PROSITEPS00601. IRF_1. 1 hit.
PS51507. IRF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio310957.
PROP97431.
SOURCESearch...

Entry information

Entry nameIRF6_MOUSE
AccessionPrimary (citable) accession number: P97431
Secondary accession number(s): Q91VD0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot