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Protein

Regulator of G-protein signaling 16

Gene

Rgs16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates G protein-coupled receptor signaling cascades (PubMed:9079700). Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:10373502). Plays an important role in the phototransduction cascade by regulating the lifetime and effective concentration of activated transducin alpha (PubMed:8917514). May regulate extra and intracellular mitogenic signals.Curated2 Publications

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • negative regulation of signal transduction Source: UniProtKB-KW
  • positive regulation of GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Signal transduction inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 16
Short name:
RGS16
Alternative name(s):
A28-RGS14P
Retinal-specific RGS
Short name:
RGS-r1 Publication
Retinally abundant regulator of G-protein signaling
Gene namesi
Name:Rgs16
Synonyms:Rgsr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:108407. Rgs16.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • intrinsic component of membrane Source: UniProtKB
  • membrane raft Source: Ensembl
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 201201Regulator of G-protein signaling 16PRO_0000204222Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21S-palmitoyl cysteine1 Publication
Lipidationi12 – 121S-palmitoyl cysteine1 Publication
Modified residuei167 – 1671Phosphotyrosine; by EGFRBy similarity
Modified residuei176 – 1761PhosphotyrosineBy similarity

Post-translational modificationi

Palmitoylated on Cys-2 and/or Cys-12.1 Publication
Phosphorylated. Phosphorylation at Tyr-167 by EGFR enhances GTPase accelerating (GAP) activity toward GNAI1.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP97428.
PRIDEiP97428.

PTM databases

iPTMnetiP97428.
PhosphoSiteiP97428.
SwissPalmiP97428.

Expressioni

Tissue specificityi

Retinal; also predominantly expressed in the liver and pituitary.

Gene expression databases

BgeeiENSMUSG00000026475.
CleanExiMM_RGS16.
ExpressionAtlasiP97428. baseline and differential.
GenevisibleiP97428. MM.

Interactioni

Subunit structurei

Interacts with GNAI1 and GNAQ (By similarity). Interacts with GNAI3, GNAI3 and GNAO1 (PubMed:9079700, PubMed:18434540).By similarity2 Publications

Protein-protein interaction databases

DIPiDIP-29922N.
IntActiP97428. 1 interaction.
MINTiMINT-1522517.
STRINGi10090.ENSMUSP00000027748.

Structurei

Secondary structure

1
201
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi58 – 636Combined sources
Helixi65 – 684Combined sources
Helixi72 – 8312Combined sources
Turni84 – 863Combined sources
Helixi88 – 10013Combined sources
Helixi106 – 12015Combined sources
Helixi133 – 14412Combined sources
Turni148 – 1514Combined sources
Helixi152 – 16312Combined sources
Helixi166 – 1716Combined sources
Helixi174 – 1774Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C7KX-ray2.90B/D53-180[»]
3C7LX-ray1.89A/B53-180[»]
ProteinModelPortaliP97428.
SMRiP97428. Positions 53-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP97428.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini64 – 180117RGSPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiP97428.
KOiK16449.
OMAiCFDAAQG.
OrthoDBiEOG091G0BDG.
PhylomeDBiP97428.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCRTLATFPN TCLERAKEFK TRLGIFLHKS ELSSDTGGIS KFEWASKHNK
60 70 80 90 100
ERSFSEDVLG WRESFDLLLN SKNGVAAFHA FLKTEFSEEN LEFWLACEEF
110 120 130 140 150
KKIRSATKLA SRAHHIFDEY IRSEAPKEVN IDHETRELTK TNLQAATTSC
160 170 180 190 200
FDVAQGKTRT LMEKDSYPRF LKSPAYRDLA AQASATSTSA PSGSPAEPSH

T
Length:201
Mass (Da):22,691
Last modified:May 1, 1997 - v1
Checksum:iAE3D798F15787392
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti51 – 511E → EN in AAC16913 (PubMed:9223279).Curated
Sequence conflicti115 – 1151H → Q in AAC16913 (PubMed:9223279).Curated
Sequence conflicti118 – 1181D → E in AAC16913 (PubMed:9223279).Curated
Sequence conflicti190 – 1901A → V in AAC16913 (PubMed:9223279).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72881 mRNA. Translation: AAC52927.1.
U67189 mRNA. Translation: AAB50619.1.
U70427 Genomic DNA. Translation: AAC16913.1.
U94828 mRNA. Translation: AAC53549.1.
BC010980 mRNA. Translation: AAH10980.1.
BC049968 mRNA. Translation: AAH49968.2.
CCDSiCCDS15376.1.
RefSeqiNP_035397.2. NM_011267.3.
UniGeneiMm.181709.

Genome annotation databases

EnsembliENSMUST00000027748; ENSMUSP00000027748; ENSMUSG00000026475.
GeneIDi19734.
KEGGimmu:19734.
UCSCiuc007dae.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72881 mRNA. Translation: AAC52927.1.
U67189 mRNA. Translation: AAB50619.1.
U70427 Genomic DNA. Translation: AAC16913.1.
U94828 mRNA. Translation: AAC53549.1.
BC010980 mRNA. Translation: AAH10980.1.
BC049968 mRNA. Translation: AAH49968.2.
CCDSiCCDS15376.1.
RefSeqiNP_035397.2. NM_011267.3.
UniGeneiMm.181709.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3C7KX-ray2.90B/D53-180[»]
3C7LX-ray1.89A/B53-180[»]
ProteinModelPortaliP97428.
SMRiP97428. Positions 53-180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29922N.
IntActiP97428. 1 interaction.
MINTiMINT-1522517.
STRINGi10090.ENSMUSP00000027748.

PTM databases

iPTMnetiP97428.
PhosphoSiteiP97428.
SwissPalmiP97428.

Proteomic databases

PaxDbiP97428.
PRIDEiP97428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027748; ENSMUSP00000027748; ENSMUSG00000026475.
GeneIDi19734.
KEGGimmu:19734.
UCSCiuc007dae.2. mouse.

Organism-specific databases

CTDi6004.
MGIiMGI:108407. Rgs16.

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiP97428.
KOiK16449.
OMAiCFDAAQG.
OrthoDBiEOG091G0BDG.
PhylomeDBiP97428.
TreeFamiTF315837.

Miscellaneous databases

ChiTaRSiRgs16. mouse.
EvolutionaryTraceiP97428.
PROiP97428.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026475.
CleanExiMM_RGS16.
ExpressionAtlasiP97428. baseline and differential.
GenevisibleiP97428. MM.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGS16_MOUSE
AccessioniPrimary (citable) accession number: P97428
Secondary accession number(s): O09091, P97420, Q80V16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.