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Protein

Regulator of G-protein signaling 16

Gene

Rgs16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulates G protein-coupled receptor signaling cascades (PubMed:9079700). Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits, thereby driving them into their inactive GDP-bound form (PubMed:10373502). Plays an important role in the phototransduction cascade by regulating the lifetime and effective concentration of activated transducin alpha (PubMed:8917514). May regulate extra and intracellular mitogenic signals.Curated2 Publications

GO - Molecular functioni

  • GTPase activator activity Source: UniProtKB

GO - Biological processi

  • G-protein coupled receptor signaling pathway Source: UniProtKB
  • negative regulation of signal transduction Source: UniProtKB-KW
  • positive regulation of GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

GTPase activation, Signal transduction inhibitor

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 16
Short name:
RGS16
Alternative name(s):
A28-RGS14P
Retinal-specific RGS
Short name:
RGS-r1 Publication
Retinally abundant regulator of G-protein signaling
Gene namesi
Name:Rgs16
Synonyms:Rgsr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:108407. Rgs16.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • intrinsic component of membrane Source: UniProtKB
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002042221 – 201Regulator of G-protein signaling 16Add BLAST201

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2S-palmitoyl cysteine1 Publication1
Lipidationi12S-palmitoyl cysteine1 Publication1
Modified residuei167Phosphotyrosine; by EGFRBy similarity1
Modified residuei176PhosphotyrosineBy similarity1

Post-translational modificationi

Palmitoylated on Cys-2 and/or Cys-12.1 Publication
Phosphorylated. Phosphorylation at Tyr-167 by EGFR enhances GTPase accelerating (GAP) activity toward GNAI1.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiP97428.
PRIDEiP97428.

PTM databases

iPTMnetiP97428.
PhosphoSitePlusiP97428.
SwissPalmiP97428.

Expressioni

Tissue specificityi

Retinal; also predominantly expressed in the liver and pituitary.

Gene expression databases

BgeeiENSMUSG00000026475.
CleanExiMM_RGS16.
ExpressionAtlasiP97428. baseline and differential.
GenevisibleiP97428. MM.

Interactioni

Subunit structurei

Interacts with GNAI1 and GNAQ (By similarity). Interacts with GNAI3, GNAI3 and GNAO1 (PubMed:9079700, PubMed:18434540).By similarity2 Publications

Protein-protein interaction databases

DIPiDIP-29922N.
IntActiP97428. 1 interactor.
MINTiMINT-1522517.
STRINGi10090.ENSMUSP00000027748.

Structurei

Secondary structure

1201
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi58 – 63Combined sources6
Helixi65 – 68Combined sources4
Helixi72 – 83Combined sources12
Turni84 – 86Combined sources3
Helixi88 – 100Combined sources13
Helixi106 – 120Combined sources15
Helixi133 – 144Combined sources12
Turni148 – 151Combined sources4
Helixi152 – 163Combined sources12
Helixi166 – 171Combined sources6
Helixi174 – 177Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C7KX-ray2.90B/D53-180[»]
3C7LX-ray1.89A/B53-180[»]
ProteinModelPortaliP97428.
SMRiP97428.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP97428.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini64 – 180RGSPROSITE-ProRule annotationAdd BLAST117

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiP97428.
KOiK16449.
OMAiCFDAAQG.
OrthoDBiEOG091G0BDG.
PhylomeDBiP97428.
TreeFamiTF315837.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCRTLATFPN TCLERAKEFK TRLGIFLHKS ELSSDTGGIS KFEWASKHNK
60 70 80 90 100
ERSFSEDVLG WRESFDLLLN SKNGVAAFHA FLKTEFSEEN LEFWLACEEF
110 120 130 140 150
KKIRSATKLA SRAHHIFDEY IRSEAPKEVN IDHETRELTK TNLQAATTSC
160 170 180 190 200
FDVAQGKTRT LMEKDSYPRF LKSPAYRDLA AQASATSTSA PSGSPAEPSH

T
Length:201
Mass (Da):22,691
Last modified:May 1, 1997 - v1
Checksum:iAE3D798F15787392
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti51E → EN in AAC16913 (PubMed:9223279).Curated1
Sequence conflicti115H → Q in AAC16913 (PubMed:9223279).Curated1
Sequence conflicti118D → E in AAC16913 (PubMed:9223279).Curated1
Sequence conflicti190A → V in AAC16913 (PubMed:9223279).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72881 mRNA. Translation: AAC52927.1.
U67189 mRNA. Translation: AAB50619.1.
U70427 Genomic DNA. Translation: AAC16913.1.
U94828 mRNA. Translation: AAC53549.1.
BC010980 mRNA. Translation: AAH10980.1.
BC049968 mRNA. Translation: AAH49968.2.
CCDSiCCDS15376.1.
RefSeqiNP_035397.2. NM_011267.3.
UniGeneiMm.181709.

Genome annotation databases

EnsembliENSMUST00000027748; ENSMUSP00000027748; ENSMUSG00000026475.
GeneIDi19734.
KEGGimmu:19734.
UCSCiuc007dae.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72881 mRNA. Translation: AAC52927.1.
U67189 mRNA. Translation: AAB50619.1.
U70427 Genomic DNA. Translation: AAC16913.1.
U94828 mRNA. Translation: AAC53549.1.
BC010980 mRNA. Translation: AAH10980.1.
BC049968 mRNA. Translation: AAH49968.2.
CCDSiCCDS15376.1.
RefSeqiNP_035397.2. NM_011267.3.
UniGeneiMm.181709.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3C7KX-ray2.90B/D53-180[»]
3C7LX-ray1.89A/B53-180[»]
ProteinModelPortaliP97428.
SMRiP97428.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29922N.
IntActiP97428. 1 interactor.
MINTiMINT-1522517.
STRINGi10090.ENSMUSP00000027748.

PTM databases

iPTMnetiP97428.
PhosphoSitePlusiP97428.
SwissPalmiP97428.

Proteomic databases

PaxDbiP97428.
PRIDEiP97428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000027748; ENSMUSP00000027748; ENSMUSG00000026475.
GeneIDi19734.
KEGGimmu:19734.
UCSCiuc007dae.2. mouse.

Organism-specific databases

CTDi6004.
MGIiMGI:108407. Rgs16.

Phylogenomic databases

eggNOGiKOG3589. Eukaryota.
ENOG410YMJD. LUCA.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233512.
HOVERGENiHBG013233.
InParanoidiP97428.
KOiK16449.
OMAiCFDAAQG.
OrthoDBiEOG091G0BDG.
PhylomeDBiP97428.
TreeFamiTF315837.

Miscellaneous databases

ChiTaRSiRgs16. mouse.
EvolutionaryTraceiP97428.
PROiP97428.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026475.
CleanExiMM_RGS16.
ExpressionAtlasiP97428. baseline and differential.
GenevisibleiP97428. MM.

Family and domain databases

Gene3Di1.10.196.10. 2 hits.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGS16_MOUSE
AccessioniPrimary (citable) accession number: P97428
Secondary accession number(s): O09091, P97420, Q80V16
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 2, 2016
This is version 141 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.