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Protein

Eosinophil cationic protein 2

Gene

Ear2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Cytotoxin and helminthotoxin with ribonuclease activity. Selectively chemotactic for dendritic cells. Possesses a wide variety of biological activities.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei38Proton acceptorBy similarity1
Active sitei151Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

  • chemotaxis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Chemotaxis

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-6803157. Antimicrobial peptides.

Names & Taxonomyi

Protein namesi
Recommended name:
Eosinophil cationic protein 2 (EC:3.1.27.-)
Short name:
ECP 2
Alternative name(s):
Eosinophil secondary granule ribonuclease 2
Short name:
EAR-2
Ribonuclease 3-2
Short name:
RNase 3-2
Gene namesi
Name:Ear2
Synonyms:Rnase2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:108020. Ear2.

Subcellular locationi

  • Cytoplasmic granule By similarity

  • Note: Matrix of eosinophil's large specific granule.By similarity

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000003086826 – 156Eosinophil cationic protein 2Add BLAST131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi47 ↔ 106By similarity
Disulfide bondi61 ↔ 119By similarity
Disulfide bondi79 ↔ 134By similarity
Disulfide bondi86 ↔ 94By similarity
Glycosylationi89N-linked (GlcNAc...)By similarity1
Glycosylationi96N-linked (GlcNAc...)Sequence analysis1
Glycosylationi107N-linked (GlcNAc...)By similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP97425.
PeptideAtlasiP97425.
PRIDEiP97425.

Expressioni

Gene expression databases

BgeeiENSMUSG00000072596.
ExpressionAtlasiP97425. baseline and differential.
GenevisibleiP97425. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074386.

Structurei

3D structure databases

ProteinModelPortaliP97425.
SMRiP97425.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni62 – 66Substrate bindingBy similarity5

Domaini

The N-terminal region is necessary for mediating chemotactic activity.

Sequence similaritiesi

Belongs to the pancreatic ribonuclease family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410JDT3. Eukaryota.
ENOG411136R. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiP97425.
OMAiRISTNCH.
OrthoDBiEOG091G0SFK.
PhylomeDBiP97425.
TreeFamiTF333393.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97425-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGPKLLESRL CLLLLLGLVL MLASCLGQTP SQWFAIQHIN NNANLQCNVE
60 70 80 90 100
MQRINRFRRT CKGLNTFLHT SFANAVGVCG NPSGLCSDNI SRNCHNSSSR
110 120 130 140 150
VRITVCNITS RRRTPYTQCR YQPRRSLEYY TVACNPRTPQ DSPMYPVVPV

HLDGTF
Length:156
Mass (Da):17,620
Last modified:May 1, 1997 - v1
Checksum:iA15E8C1133091053
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72031 mRNA. Translation: AAB37785.1.
AF306664 Genomic DNA. Translation: AAG25991.1.
AF306665 mRNA. Translation: AAG25992.1.
BC065391 mRNA. Translation: AAH65391.1.
BC094626 mRNA. Translation: AAH94626.1.
CCDSiCCDS26968.1.
PIRiJC6160.
RefSeqiNP_031921.1. NM_007895.2.
UniGeneiMm.327386.

Genome annotation databases

EnsembliENSMUST00000074839; ENSMUSP00000074386; ENSMUSG00000072596.
GeneIDi13587.
KEGGimmu:13587.
UCSCiuc007tdi.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U72031 mRNA. Translation: AAB37785.1.
AF306664 Genomic DNA. Translation: AAG25991.1.
AF306665 mRNA. Translation: AAG25992.1.
BC065391 mRNA. Translation: AAH65391.1.
BC094626 mRNA. Translation: AAH94626.1.
CCDSiCCDS26968.1.
PIRiJC6160.
RefSeqiNP_031921.1. NM_007895.2.
UniGeneiMm.327386.

3D structure databases

ProteinModelPortaliP97425.
SMRiP97425.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000074386.

Proteomic databases

PaxDbiP97425.
PeptideAtlasiP97425.
PRIDEiP97425.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000074839; ENSMUSP00000074386; ENSMUSG00000072596.
GeneIDi13587.
KEGGimmu:13587.
UCSCiuc007tdi.1. mouse.

Organism-specific databases

CTDi13587.
MGIiMGI:108020. Ear2.

Phylogenomic databases

eggNOGiENOG410JDT3. Eukaryota.
ENOG411136R. LUCA.
GeneTreeiENSGT00840000129759.
HOGENOMiHOG000276882.
HOVERGENiHBG008396.
InParanoidiP97425.
OMAiRISTNCH.
OrthoDBiEOG091G0SFK.
PhylomeDBiP97425.
TreeFamiTF333393.

Enzyme and pathway databases

ReactomeiR-MMU-6798695. Neutrophil degranulation.
R-MMU-6803157. Antimicrobial peptides.

Miscellaneous databases

PROiP97425.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000072596.
ExpressionAtlasiP97425. baseline and differential.
GenevisibleiP97425. MM.

Family and domain databases

Gene3Di3.10.130.10. 1 hit.
InterProiIPR001427. RNaseA.
IPR023411. RNaseA_AS.
IPR023412. RNaseA_domain.
[Graphical view]
PANTHERiPTHR11437. PTHR11437. 1 hit.
PfamiPF00074. RnaseA. 1 hit.
[Graphical view]
PRINTSiPR00794. RIBONUCLEASE.
ProDomiPD000535. RNaseA. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00092. RNAse_Pc. 1 hit.
[Graphical view]
SUPFAMiSSF54076. SSF54076. 1 hit.
PROSITEiPS00127. RNASE_PANCREATIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiECP2_MOUSE
AccessioniPrimary (citable) accession number: P97425
Secondary accession number(s): Q505C0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.