P97414 (KCNQ1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Potassium voltage-gated channel subfamily KQT member 1

Alternative name(s):
IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1
KQT-like 1
Voltage-gated potassium channel subunit Kv7.1

Gene names

Name:	Kcnq1
Synonyms:	Kcna9, Kvlqt1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	668 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably important in cardiac repolarization. Associates with KCNE1 (MinK) to form the I(Ks) cardiac potassium current. Elicits a rapidly activating, potassium-selective outward current.
Subunit structure	Heterotetramer with KCNE1 (MinK) or KCNE3 (MiRP2). Interacts with CALM By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein By similarity.
Tissue specificity	Expressed in heart, kidney and salivary glands. Detected in the cochlea. Almost undetectable in brain, skeletal muscle and liver. Widely expressed in embryonic and neonatal tissues. Ref.5
Domain	The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
Sequence similarities	Belongs to the potassium channel family. KQT (TC 1.A.1.15) subfamily. Kv7.1/KCNQ1 sub-subfamily. [View classification]
Sequence caution	The sequence AAB36518.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Domain	Transmembrane Transmembrane helix
Ligand	Calmodulin-binding Potassium
Molecular function	Ion channel Potassium channel Voltage-gated channel
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cardiovascular system development Inferred from mutant phenotype Ref.1. Source: MGI gene silencing Inferred from mutant phenotype PubMed 15340049. Source: MGI negative regulation of insulin secretion Inferred from electronic annotation. Source: Compara potassium ion export Inferred from electronic annotation. Source: Compara regulation of gene expression by genetic imprinting Inferred from mutant phenotype PubMed 12410230PubMed 15340049. Source: MGI regulation of membrane repolarization Inferred from electronic annotation. Source: Compara response to chemical stimulus Inferred from electronic annotation. Source: Compara
Cellular_component	basolateral plasma membrane Inferred from electronic annotation. Source: Compara early endosome Inferred from electronic annotation. Source: Compara late endosome Inferred from electronic annotation. Source: Compara lysosome Inferred from electronic annotation. Source: Compara sarcolemma Inferred from electronic annotation. Source: Compara voltage-gated potassium channel complex Inferred from electronic annotation. Source: Compara zymogen granule membrane Inferred from electronic annotation. Source: Compara
Molecular_function	delayed rectifier potassium channel activity Inferred from Biological aspect of Ancestor. Source: RefGenome outward rectifier potassium channel activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform I (identifier: P97414-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform II (identifier: P97414-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-111: Missing.
     112-128: FLERPTGWKCFVYHFTV → MHQLPTSILIPKNMPGG

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 668	668	Potassium voltage-gated channel subfamily KQT member 1		PRO_0000054024
Regions
	Transmembrane	121 – 141	21	Helical; Name=Segment S1; Potential
	Topological domain	142 – 146	5	Extracellular Potential
	Transmembrane	147 – 167	21	Helical; Name=Segment S2; Potential
	Topological domain	168 – 195	28	Cytoplasmic Potential
	Transmembrane	196 – 216	21	Helical; Name=Segment S3; Potential
	Topological domain	217 – 224	8	Extracellular Potential
	Transmembrane	225 – 247	23	Helical; Voltage-sensor; Name=Segment S4; Potential
	Topological domain	248 – 260	13	Cytoplasmic Potential
	Transmembrane	261 – 281	21	Helical; Name=Segment S5; Potential
	Topological domain	282 – 298	17	Extracellular Potential
	Intramembrane	299 – 319	21	Pore-forming; Name=Segment H5; Potential
	Topological domain	320 – 330	11	Extracellular Potential
	Transmembrane	331 – 351	21	Helical; Name=Segment S6; Potential
	Topological domain	352 – 668	317	Cytoplasmic Potential
	Motif	311 – 316	6	Selectivity filter By similarity
Amino acid modifications
	Glycosylation	288	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 111	111	Missing in isoform II.		VSP_011748
	Alternative sequence	112 – 128	17	FLERP…YHFTV → MHQLPTSILIPKNMPGG in isoform II.		VSP_000983
Experimental info
	Sequence conflict	473	1	V → L in AAP93874. Ref.1
	Sequence conflict	473	1	V → L in AAB36518. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform I [UniParc]. Last modified July 27, 2011. Version 3. Checksum: 8CE35CBA3102B85B	FASTA	668	74,514
	10 20 30 40 50 60 MDTASSPPSA ERKRAGWSRL LGARRGSAVV KKCPFSLELA EGGPEGSTVY APIAPTGAPG 70 80 90 100 110 120 LAPPMSTPVS PAPAPADLGP RPRVSLDPRV SIYSARRPLL ARTHIQGRVY NFLERPTGWK 130 140 150 160 170 180 CFVYHFTVFL IVLVCLIFSV LSTIEQYAAL ATGTLFWMEI VLVVFFGTEY VVRLWSAGCR 190 200 210 220 230 240 SKYVGIWGRL RFARKPISII DLIVVVASMV VLCVGSKGQV FATSAIRGIR FLQILRMLHV 250 260 270 280 290 300 DRQGGTWRLL GSVVFIHRQE LITTLYIGFL GLIFSSYFVY LAEKDAVNES GRIEFGSYAD 310 320 330 340 350 360 ALWWGVVTVT TIGYGDKVPQ TWVGKTIASC FSVFAISFFA LPAGILGSGF ALKVQQKQRQ 370 380 390 400 410 420 KHFNRQIPAA ASLIQTAWRC YAAENPDSAT WKIYVRKPAR SHTLLSPSPK PKKSVMVKKK 430 440 450 460 470 480 KFKLDKDNGM SPGEKMFNVP HITYDPPEDR RPDHFSIDGY DSSVRKSPTL LEVSTPHFLR 490 500 510 520 530 540 TNSFAEDLDL EGETLLTPIT HVSQLRDHHR ATIKVIRRMQ YFVAKKKFQQ ARKPYDVRDV 550 560 570 580 590 600 IEQYSQGHLN LMVRIKELQR RLDQSIGKPS LFIPISEKSK DRGSNTIGAR LNRVEDKVTQ 610 620 630 640 650 660 LDQRLVIITD MLHQLLSMQQ GGPTCNSRSQ VVASNEGGSI NPELFLPSNS LPTYEQLTVP QTGPDEGS
	Isoform II [UniParc]. Checksum: 56D3B3EF5F8D0FA0	FASTA	557	62,640
	10 20 30 40 50 60 MHQLPTSILI PKNMPGGFLI VLVCLIFSVL STIEQYAALA TGTLFWMEIV LVVFFGTEYV 70 80 90 100 110 120 VRLWSAGCRS KYVGIWGRLR FARKPISIID LIVVVASMVV LCVGSKGQVF ATSAIRGIRF 130 140 150 160 170 180 LQILRMLHVD RQGGTWRLLG SVVFIHRQEL ITTLYIGFLG LIFSSYFVYL AEKDAVNESG 190 200 210 220 230 240 RIEFGSYADA LWWGVVTVTT IGYGDKVPQT WVGKTIASCF SVFAISFFAL PAGILGSGFA 250 260 270 280 290 300 LKVQQKQRQK HFNRQIPAAA SLIQTAWRCY AAENPDSATW KIYVRKPARS HTLLSPSPKP 310 320 330 340 350 360 KKSVMVKKKK FKLDKDNGMS PGEKMFNVPH ITYDPPEDRR PDHFSIDGYD SSVRKSPTLL 370 380 390 400 410 420 EVSTPHFLRT NSFAEDLDLE GETLLTPITH VSQLRDHHRA TIKVIRRMQY FVAKKKFQQA 430 440 450 460 470 480 RKPYDVRDVI EQYSQGHLNL MVRIKELQRR LDQSIGKPSL FIPISEKSKD RGSNTIGARL 490 500 510 520 530 540 NRVEDKVTQL DQRLVIITDM LHQLLSMQQG GPTCNSRSQV VASNEGGSIN PELFLPSNSL 550 PTYEQLTVPQ TGPDEGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isoproterenol exacerbates a long QT phenotype in Kcnq1-deficient neonatal mice: possible roles for human-like Kcnq1 isoform 1 and slow delayed rectifier K+ current." Knollmann B.C., Casimiro M.C., Katchman A.N., Sirenko S.G., Schober T., Rong Q., Pfeifer K., Ebert S.N. J. Pharmacol. Exp. Ther. 310:311-318(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM I). Strain: 129/SvJ. Tissue: Heart.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I). Strain: NOD.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM I). Strain: FVB/N. Tissue: Colon and Olfactory epithelium.
[4]	"K(V)LQT1 and IsK (minK) proteins associate to form the I(Ks) cardiac potassium current." Barhanin J., Lesage F., Guillemare E., Fink M., Lazdunski M., Romey G. Nature 384:78-80(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 31-668 (ISOFORM I). Tissue: Heart.
[5]	"Syntenic organization of the mouse distal chromosome 7 imprinting cluster and the Beckwith-Wiedemann syndrome region in chromosome 11p15.5." Paulsen M., Davies K.R., Bowden L.M., Villar A.J., Franck O., Fuermann M., Dean W.L., Moore T.F., Rodrigues N., Davies K.E., Hu R.-J., Feinberg A.P., Maher E.R., Reik W., Walter J. Hum. Mol. Genet. 7:1149-1159(1998) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE (ISOFORM II), TISSUE SPECIFICITY. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY331142 mRNA. Translation: AAP93874.1. AK154244 mRNA. Translation: BAE32460.1. BC045142 mRNA. Translation: AAH45142.2. BC055304 mRNA. Translation: AAH55304.1. U70068 mRNA. Translation: AAB36518.1. Different initiation. AJ002201 mRNA. Translation: CAA05246.1.
IPI	IPI00125237. IPI00652261.
RefSeq	NP_032460.2. NM_008434.2.
UniGene	Mm.439769. Mm.453036. Mm.453037.
3D structure databases
ProteinModelPortal	P97414.
SMR	P97414. Positions 146-353, 503-528, 584-610.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000009689.
Protein family/group databases
TCDB	1.A.1.15.1. voltage-gated ion channel (VIC) superfamily.
PTM databases
PhosphoSite	P97414.
Proteomic databases
PaxDb	P97414.
PRIDE	P97414.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000009689; ENSMUSP00000009689; ENSMUSG00000009545.
GeneID	16535.
KEGG	mmu:16535.
Organism-specific databases
CTD	3784.
MGI	MGI:108083. Kcnq1.
Phylogenomic databases
eggNOG	COG1226.
GeneTree	ENSGT00550000074513.
HOGENOM	HOG000220839.
HOVERGEN	HBG059014.
InParanoid	Q3U4H1.
KO	K04926.
OMA	GWGRLPG.
OrthoDB	EOG4Q58PW.
Gene expression databases
ArrayExpress	P97414.
Bgee	P97414.
Genevestigator	P97414.
GermOnline	ENSMUSG00000009545. Mus musculus.
Family and domain databases
InterPro	IPR005821. Ion_trans_dom. IPR003091. K_chnl. IPR003937. K_chnl_volt-dep_KCNQ. IPR013821. K_chnl_volt-dep_KCNQ_C. IPR005827. K_chnl_volt-dep_KCQN1. [Graphical view]
PANTHER	PTHR11537. PTHR11537. 1 hit. PTHR11537:SF9. PTHR11537:SF9. 1 hit.
Pfam	PF00520. Ion_trans. 1 hit. PF03520. KCNQ_channel. 1 hit. [Graphical view]
PRINTS	PR00169. KCHANNEL. PR01460. KCNQ1CHANNEL. PR01459. KCNQCHANNEL.
ProtoNet	Search...
Other
NextBio	289961.
SOURCE	Search...

Entry information

Entry name

KCNQ1_MOUSE

Accession

Primary (citable) accession number: P97414
Secondary accession number(s): O88702 , Q3U4H1, Q7TNZ1, Q7TPL7, Q80VR7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 119 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program