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P97402

- GCNT2_MOUSE

UniProt

P97402 - GCNT2_MOUSE

Protein

N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase

Gene

Gcnt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 102 (01 Oct 2014)
      Sequence version 2 (18 Sep 2013)
      Previous versions | rss
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    Functioni

    Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells.

    Catalytic activityi

    UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

    Pathwayi

    GO - Molecular functioni

    1. acetylglucosaminyltransferase activity Source: MGI
    2. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. positive regulation of cell proliferation Source: UniProt
    2. positive regulation of epithelial to mesenchymal transition Source: UniProt
    3. positive regulation of ERK1 and ERK2 cascade Source: UniProt
    4. positive regulation of protein kinase B signaling Source: UniProt
    5. posttranscriptional regulation of gene expression Source: UniProt
    6. protein glycosylation Source: UniProtKB-UniPathway
    7. transforming growth factor beta receptor signaling pathway Source: UniProt

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT14. Glycosyltransferase Family 14.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase (EC:2.4.1.150)
    Short name:
    N-acetylglucosaminyltransferase
    Alternative name(s):
    I-branching enzyme
    IGNT
    Large I antigen-forming beta-1,6-N-acetylglucosaminyltransferase
    Gene namesi
    Name:Gcnt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 13

    Organism-specific databases

    MGIiMGI:1100870. Gcnt2.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 401401N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferasePRO_0000191398Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiP97402.

    PTM databases

    PhosphoSiteiP97402.

    Expressioni

    Gene expression databases

    GenevestigatoriP97402.

    Structurei

    3D structure databases

    ProteinModelPortaliP97402.
    SMRiP97402. Positions 55-388.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 77CytoplasmicSequence Analysis
    Topological domaini29 – 401373LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 14 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG253565.
    GeneTreeiENSGT00750000117400.
    HOVERGENiHBG051711.
    KOiK00742.

    Family and domain databases

    InterProiIPR003406. Glyco_trans_14.
    [Graphical view]
    PfamiPF02485. Branch. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97402-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPSVRYFFI VSVTTVIVFI VLYVLSFGGD QSYQKLNISD SVMLAQVCSS    50
    FIDGKSRFLW RNKLMIHEKP SCTEYVTQSH YITAPLSQEE VDFPLAYVMV 100
    IHHNFDTFAR LFRAIFMPQN IYCVHVDEKA TAEFKGAVEQ LVSCFPNAFL 150
    ASKMEPVVYG GISRLQADLN CIKDLSTSEV PWKYAINTCG QDFPLKTNKE 200
    IVQYLKGLKG KNLTPGVLPP AHAIGRTRYV HREHLSKELS YVIRTTALKP 250
    PPPHNLTIYF GSAYVALSRE FANFVLRDPR AVDLLHWSKD TFSPDEHFWV 300
    TLNRIPGVPG SMPPNASWTG NLRAVKWMDM EAKHGGCHGH YVHGICIYGN 350
    GDLQWLINSQ SLFANKFELN TYPLTVECLE LRLRERTLNQ SEIAIQPSWY 400
    F 401
    Length:401
    Mass (Da):45,697
    Last modified:September 18, 2013 - v2
    Checksum:i4DBD498E6EEEDF13
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31P → L in AAB39621. (PubMed:9134435)Curated
    Sequence conflicti17 – 226IVFIVL → MVCVVS in AAB39621. (PubMed:9134435)Curated
    Sequence conflicti43 – 453MLA → RLS in AAB39621. (PubMed:9134435)Curated
    Sequence conflicti91 – 911V → G in AAB39621. (PubMed:9134435)Curated
    Sequence conflicti148 – 1481A → V in AAB39621. (PubMed:9134435)Curated
    Sequence conflicti312 – 3121Missing in AAB39621. (PubMed:9134435)Curated
    Sequence conflicti338 – 3381H → Q in AAB39621. (PubMed:9134435)Curated
    Sequence conflicti393 – 3931I → S in AAB39621. (PubMed:9134435)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U68182 mRNA. Translation: AAB39621.1.
    AY435149 mRNA. Translation: AAR95650.1.
    AC133496 Genomic DNA. No translation available.
    AC159205 Genomic DNA. No translation available.
    CCDSiCCDS26468.1.
    RefSeqiNP_032131.2. NM_008105.3.
    UniGeneiMm.314757.

    Genome annotation databases

    EnsembliENSMUST00000069958; ENSMUSP00000070942; ENSMUSG00000021360.
    GeneIDi14538.
    KEGGimmu:14538.
    UCSCiuc007qem.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Gcnt2 - I branching beta-6-GlcNAcT2, variant 3 (IGnT)

    Functional Glycomics Gateway - GTase

    Gcnt2 - I branching beta-6-GlcNAcT2, variant 1 (IGnT)

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U68182 mRNA. Translation: AAB39621.1 .
    AY435149 mRNA. Translation: AAR95650.1 .
    AC133496 Genomic DNA. No translation available.
    AC159205 Genomic DNA. No translation available.
    CCDSi CCDS26468.1.
    RefSeqi NP_032131.2. NM_008105.3.
    UniGenei Mm.314757.

    3D structure databases

    ProteinModelPortali P97402.
    SMRi P97402. Positions 55-388.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT14. Glycosyltransferase Family 14.

    PTM databases

    PhosphoSitei P97402.

    Proteomic databases

    PRIDEi P97402.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000069958 ; ENSMUSP00000070942 ; ENSMUSG00000021360 .
    GeneIDi 14538.
    KEGGi mmu:14538.
    UCSCi uc007qem.2. mouse.

    Organism-specific databases

    CTDi 2651.
    MGIi MGI:1100870. Gcnt2.

    Phylogenomic databases

    eggNOGi NOG253565.
    GeneTreei ENSGT00750000117400.
    HOVERGENi HBG051711.
    KOi K00742.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 13845122.
    PROi P97402.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori P97402.

    Family and domain databases

    InterProi IPR003406. Glyco_trans_14.
    [Graphical view ]
    Pfami PF02485. Branch. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Expression of the large I antigen forming beta-1,6-N-acetylglucosaminyltransferase in various tissues of adult mice."
      Magnet A.D., Fukuda M.
      Glycobiology 7:285-295(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Multiple variable first exons: a mechanism for cell- and tissue-specific gene regulation."
      Zhang T., Haws P., Wu Q.
      Genome Res. 14:79-89(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiGCNT2_MOUSE
    AccessioniPrimary (citable) accession number: P97402
    Secondary accession number(s): Q6T5E3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: September 18, 2013
    Last modified: October 1, 2014
    This is version 102 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3