P97402 (GCNT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
Short name=N-acetylglucosaminyltransferase
EC=2.4.1.150

Alternative name(s):
I-branching enzyme
IGNT
Large I antigen-forming beta-1,6-N-acetylglucosaminyltransferase

Gene names

Name:

Gcnt2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells.
Catalytic activity	UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 14 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	protein glycosylation Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC acetylglucosaminyltransferase activity Inferred from direct assay PubMed 12670491. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase		PRO_0000191398
Regions
	Topological domain	1 – 6	6	Cytoplasmic Potential
	Transmembrane	7 – 25	19	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	26 – 400	375	Lumenal Potential
Amino acid modifications
	Glycosylation	37	1	N-linked (GlcNAc...) Potential
	Glycosylation	255	1	N-linked (GlcNAc...) Potential
	Glycosylation	314	1	N-linked (GlcNAc...) Potential
	Glycosylation	388	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P97402 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 193FF3882E3B9691	FASTA	400	45,507
	10 20 30 40 50 60 MPLSVRYFFI VSVTTVMVCV VSYVLSFGGD QSYQKLNISD SVRLSQVCSS FIDGKSRFLW 70 80 90 100 110 120 RNKLMIHEKP SCTEYVTQSH YITAPLSQEE GDFPLAYVMV IHHNFDTFAR LFRAIFMPQN 130 140 150 160 170 180 IYCVHVDEKA TAEFKGAVEQ LVSCFPNVFL ASKMEPVVYG GISRLQADLN CIKDLSTSEV 190 200 210 220 230 240 PWKYAINTCG QDFPLKTNKE IVQYLKGLKG KNLTPGVLPP AHAIGRTRYV HREHLSKELS 250 260 270 280 290 300 YVIRTTALKP PPPHNLTIYF GSAYVALSRE FANFVLRDPR AVDLLHWSKD TFSPDEHFWV 310 320 330 340 350 360 TLNRIPGVPG SPPNASWTGN LRAVKWMDME AKHGGCQGHY VHGICIYGNG DLQWLINSQS 370 380 390 400 LFANKFELNT YPLTVECLEL RLRERTLNQS ESAIQPSWYF

References

[1]	"Expression of the large I antigen forming beta-1,6-N-acetylglucosaminyltransferase in various tissues of adult mice." Magnet A.D., Fukuda M. Glycobiology 7:285-295(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Gcnt2 - I branching beta-6-GlcNAcT2, variant 3 (IGnT)

Functional Glycomics Gateway - GTase

Gcnt2 - I branching beta-6-GlcNAcT2, variant 1 (IGnT)

Cross-references

Sequence databases
EMBL GenBank DDBJ	U68182 mRNA. Translation: AAB39621.1.
IPI	IPI00125167.
UniGene	Mm.314757.
3D structure databases
ProteinModelPortal	P97402.
SMR	P97402. Positions 55-387.
ModBase	Search...
Protein family/group databases
CAZy	GT14. Glycosyltransferase Family 14.
PTM databases
PhosphoSite	P97402.
Proteomic databases
PRIDE	P97402.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:1100870. Gcnt2.
Phylogenomic databases
eggNOG	NOG253565.
HOVERGEN	HBG051711.
Enzyme and pathway databases
UniPathway	UPA00378.
Gene expression databases
Genevestigator	P97402.
GermOnline	ENSMUSG00000021360. Mus musculus.
Family and domain databases
InterPro	IPR003406. Glyco_trans_14. [Graphical view]
Pfam	PF02485. Branch. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	13845122.
SOURCE	Search...

Entry information

Entry name

GCNT2_MOUSE

Accession

Primary (citable) accession number: P97402

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program