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P97402 (GCNT2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase

Short name=N-acetylglucosaminyltransferase
EC=2.4.1.150
Alternative name(s):
I-branching enzyme
IGNT
Large I antigen-forming beta-1,6-N-acetylglucosaminyltransferase
Gene names
Name:Gcnt2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length401 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells.

Catalytic activity

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein.

Sequence similarities

Belongs to the glycosyltransferase 14 family.

Ontologies

Keywords
   Cellular componentGolgi apparatus
Membrane
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   Molecular functionGlycosyltransferase
Transferase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processpositive regulation of ERK1 and ERK2 cascade

Inferred from mutant phenotype PubMed 21750175. Source: UniProt

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 21750175. Source: UniProt

positive regulation of epithelial to mesenchymal transition

Inferred from mutant phenotype PubMed 21750175. Source: UniProt

positive regulation of protein kinase B signaling

Inferred from mutant phenotype PubMed 21750175. Source: UniProt

posttranscriptional regulation of gene expression

Inferred from mutant phenotype PubMed 21750175. Source: UniProt

protein glycosylation

Inferred from electronic annotation. Source: UniProtKB-UniPathway

transforming growth factor beta receptor signaling pathway

Inferred from mutant phenotype PubMed 21750175. Source: UniProt

   Cellular_componentGolgi membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionN-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

acetylglucosaminyltransferase activity

Inferred from direct assay PubMed 12670491. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 401401N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase
PRO_0000191398

Regions

Topological domain1 – 77Cytoplasmic Potential
Transmembrane8 – 2821Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 401373Lumenal Potential

Amino acid modifications

Glycosylation371N-linked (GlcNAc...) Potential
Glycosylation2551N-linked (GlcNAc...) Potential
Glycosylation3151N-linked (GlcNAc...) Potential
Glycosylation3891N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict31P → L in AAB39621. Ref.1
Sequence conflict17 – 226IVFIVL → MVCVVS in AAB39621. Ref.1
Sequence conflict43 – 453MLA → RLS in AAB39621. Ref.1
Sequence conflict911V → G in AAB39621. Ref.1
Sequence conflict1481A → V in AAB39621. Ref.1
Sequence conflict3121Missing in AAB39621. Ref.1
Sequence conflict3381H → Q in AAB39621. Ref.1
Sequence conflict3931I → S in AAB39621. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P97402 [UniParc].

Last modified September 18, 2013. Version 2.
Checksum: 4DBD498E6EEEDF13

FASTA40145,697
        10         20         30         40         50         60 
MPPSVRYFFI VSVTTVIVFI VLYVLSFGGD QSYQKLNISD SVMLAQVCSS FIDGKSRFLW 

        70         80         90        100        110        120 
RNKLMIHEKP SCTEYVTQSH YITAPLSQEE VDFPLAYVMV IHHNFDTFAR LFRAIFMPQN 

       130        140        150        160        170        180 
IYCVHVDEKA TAEFKGAVEQ LVSCFPNAFL ASKMEPVVYG GISRLQADLN CIKDLSTSEV 

       190        200        210        220        230        240 
PWKYAINTCG QDFPLKTNKE IVQYLKGLKG KNLTPGVLPP AHAIGRTRYV HREHLSKELS 

       250        260        270        280        290        300 
YVIRTTALKP PPPHNLTIYF GSAYVALSRE FANFVLRDPR AVDLLHWSKD TFSPDEHFWV 

       310        320        330        340        350        360 
TLNRIPGVPG SMPPNASWTG NLRAVKWMDM EAKHGGCHGH YVHGICIYGN GDLQWLINSQ 

       370        380        390        400 
SLFANKFELN TYPLTVECLE LRLRERTLNQ SEIAIQPSWY F 

« Hide

References

« Hide 'large scale' references
[1]"Expression of the large I antigen forming beta-1,6-N-acetylglucosaminyltransferase in various tissues of adult mice."
Magnet A.D., Fukuda M.
Glycobiology 7:285-295(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Multiple variable first exons: a mechanism for cell- and tissue-specific gene regulation."
Zhang T., Haws P., Wu Q.
Genome Res. 14:79-89(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
+Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Gcnt2 - I branching beta-6-GlcNAcT2, variant 3 (IGnT)

Functional Glycomics Gateway - GTase

Gcnt2 - I branching beta-6-GlcNAcT2, variant 1 (IGnT)

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U68182 mRNA. Translation: AAB39621.1.
AY435149 mRNA. Translation: AAR95650.1.
AC133496 Genomic DNA. No translation available.
AC159205 Genomic DNA. No translation available.
CCDSCCDS26468.1.
RefSeqNP_032131.2. NM_008105.3.
UniGeneMm.314757.

3D structure databases

ProteinModelPortalP97402.
SMRP97402. Positions 55-388.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT14. Glycosyltransferase Family 14.

PTM databases

PhosphoSiteP97402.

Proteomic databases

PRIDEP97402.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000069958; ENSMUSP00000070942; ENSMUSG00000021360.
GeneID14538.
KEGGmmu:14538.
UCSCuc007qem.2. mouse.

Organism-specific databases

CTD2651.
MGIMGI:1100870. Gcnt2.

Phylogenomic databases

eggNOGNOG253565.
GeneTreeENSGT00750000117400.
HOVERGENHBG051711.
KOK00742.

Enzyme and pathway databases

UniPathwayUPA00378.

Gene expression databases

GenevestigatorP97402.

Family and domain databases

InterProIPR003406. Glyco_trans_14.
[Graphical view]
PfamPF02485. Branch. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio13845122.
PROP97402.
SOURCESearch...

Entry information

Entry nameGCNT2_MOUSE
AccessionPrimary (citable) accession number: P97402
Secondary accession number(s): Q6T5E3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 18, 2013
Last modified: July 9, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot