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P97402

- GCNT2_MOUSE

UniProt

P97402 - GCNT2_MOUSE

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Protein

N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase

Gene

Gcnt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Branching enzyme that converts linear into branched poly-N-acetyllactosaminoglycans. Introduces the blood group I antigen during embryonic development. It is closely associated with the development and maturation of erythroid cells.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-1,6-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R.

Pathwayi

GO - Molecular functioni

  1. acetylglucosaminyltransferase activity Source: MGI
  2. N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. maintenance of lens transparency Source: Ensembl
  2. negative regulation of cell-substrate adhesion Source: Ensembl
  3. positive regulation of cell migration Source: Ensembl
  4. positive regulation of cell proliferation Source: UniProt
  5. positive regulation of epithelial to mesenchymal transition Source: UniProt
  6. positive regulation of ERK1 and ERK2 cascade Source: UniProt
  7. positive regulation of heterotypic cell-cell adhesion Source: Ensembl
  8. positive regulation of protein kinase B signaling Source: UniProt
  9. posttranscriptional regulation of gene expression Source: UniProt
  10. protein glycosylation Source: UniProtKB-UniPathway
  11. transforming growth factor beta receptor signaling pathway Source: UniProt
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT14. Glycosyltransferase Family 14.

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferase (EC:2.4.1.150)
Short name:
N-acetylglucosaminyltransferase
Alternative name(s):
I-branching enzyme
IGNT
Large I antigen-forming beta-1,6-N-acetylglucosaminyltransferase
Gene namesi
Name:Gcnt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 13

Organism-specific databases

MGIiMGI:1100870. Gcnt2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 77CytoplasmicSequence Analysis
Transmembranei8 – 2821Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 401373LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 401401N-acetyllactosaminide beta-1,6-N-acetylglucosaminyl-transferasePRO_0000191398Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi37 – 371N-linked (GlcNAc...)Sequence Analysis
Glycosylationi255 – 2551N-linked (GlcNAc...)Sequence Analysis
Glycosylationi315 – 3151N-linked (GlcNAc...)Sequence Analysis
Glycosylationi389 – 3891N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP97402.

PTM databases

PhosphoSiteiP97402.

Expressioni

Gene expression databases

ExpressionAtlasiP97402. baseline and differential.
GenevestigatoriP97402.

Structurei

3D structure databases

ProteinModelPortaliP97402.
SMRiP97402. Positions 55-388.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 14 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG253565.
GeneTreeiENSGT00760000119183.
HOVERGENiHBG051711.
InParanoidiP97402.
KOiK00742.

Family and domain databases

InterProiIPR003406. Glyco_trans_14.
[Graphical view]
PfamiPF02485. Branch. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97402-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPPSVRYFFI VSVTTVIVFI VLYVLSFGGD QSYQKLNISD SVMLAQVCSS
60 70 80 90 100
FIDGKSRFLW RNKLMIHEKP SCTEYVTQSH YITAPLSQEE VDFPLAYVMV
110 120 130 140 150
IHHNFDTFAR LFRAIFMPQN IYCVHVDEKA TAEFKGAVEQ LVSCFPNAFL
160 170 180 190 200
ASKMEPVVYG GISRLQADLN CIKDLSTSEV PWKYAINTCG QDFPLKTNKE
210 220 230 240 250
IVQYLKGLKG KNLTPGVLPP AHAIGRTRYV HREHLSKELS YVIRTTALKP
260 270 280 290 300
PPPHNLTIYF GSAYVALSRE FANFVLRDPR AVDLLHWSKD TFSPDEHFWV
310 320 330 340 350
TLNRIPGVPG SMPPNASWTG NLRAVKWMDM EAKHGGCHGH YVHGICIYGN
360 370 380 390 400
GDLQWLINSQ SLFANKFELN TYPLTVECLE LRLRERTLNQ SEIAIQPSWY

F
Length:401
Mass (Da):45,697
Last modified:September 18, 2013 - v2
Checksum:i4DBD498E6EEEDF13
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31P → L in AAB39621. (PubMed:9134435)Curated
Sequence conflicti17 – 226IVFIVL → MVCVVS in AAB39621. (PubMed:9134435)Curated
Sequence conflicti43 – 453MLA → RLS in AAB39621. (PubMed:9134435)Curated
Sequence conflicti91 – 911V → G in AAB39621. (PubMed:9134435)Curated
Sequence conflicti148 – 1481A → V in AAB39621. (PubMed:9134435)Curated
Sequence conflicti312 – 3121Missing in AAB39621. (PubMed:9134435)Curated
Sequence conflicti338 – 3381H → Q in AAB39621. (PubMed:9134435)Curated
Sequence conflicti393 – 3931I → S in AAB39621. (PubMed:9134435)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68182 mRNA. Translation: AAB39621.1.
AY435149 mRNA. Translation: AAR95650.1.
AC133496 Genomic DNA. No translation available.
AC159205 Genomic DNA. No translation available.
CCDSiCCDS26468.1.
RefSeqiNP_032131.2. NM_008105.3.
UniGeneiMm.314757.

Genome annotation databases

EnsembliENSMUST00000069958; ENSMUSP00000070942; ENSMUSG00000021360.
GeneIDi14538.
KEGGimmu:14538.
UCSCiuc007qem.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

Gcnt2 - I branching beta-6-GlcNAcT2, variant 3 (IGnT)

Functional Glycomics Gateway - GTase

Gcnt2 - I branching beta-6-GlcNAcT2, variant 1 (IGnT)

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68182 mRNA. Translation: AAB39621.1 .
AY435149 mRNA. Translation: AAR95650.1 .
AC133496 Genomic DNA. No translation available.
AC159205 Genomic DNA. No translation available.
CCDSi CCDS26468.1.
RefSeqi NP_032131.2. NM_008105.3.
UniGenei Mm.314757.

3D structure databases

ProteinModelPortali P97402.
SMRi P97402. Positions 55-388.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT14. Glycosyltransferase Family 14.

PTM databases

PhosphoSitei P97402.

Proteomic databases

PRIDEi P97402.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000069958 ; ENSMUSP00000070942 ; ENSMUSG00000021360 .
GeneIDi 14538.
KEGGi mmu:14538.
UCSCi uc007qem.2. mouse.

Organism-specific databases

CTDi 2651.
MGIi MGI:1100870. Gcnt2.

Phylogenomic databases

eggNOGi NOG253565.
GeneTreei ENSGT00760000119183.
HOVERGENi HBG051711.
InParanoidi P97402.
KOi K00742.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

ChiTaRSi Gcnt2. mouse.
NextBioi 13845122.
PROi P97402.
SOURCEi Search...

Gene expression databases

ExpressionAtlasi P97402. baseline and differential.
Genevestigatori P97402.

Family and domain databases

InterProi IPR003406. Glyco_trans_14.
[Graphical view ]
Pfami PF02485. Branch. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of the large I antigen forming beta-1,6-N-acetylglucosaminyltransferase in various tissues of adult mice."
    Magnet A.D., Fukuda M.
    Glycobiology 7:285-295(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Multiple variable first exons: a mechanism for cell- and tissue-specific gene regulation."
    Zhang T., Haws P., Wu Q.
    Genome Res. 14:79-89(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiGCNT2_MOUSE
AccessioniPrimary (citable) accession number: P97402
Secondary accession number(s): Q6T5E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: September 18, 2013
Last modified: November 26, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3