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P97401

- SFRP3_MOUSE

UniProt

P97401 - SFRP3_MOUSE

Protein

Secreted frizzled-related protein 3

Gene

Frzb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 135 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP3/FRZB appears to be involved in limb skeletogenesis. Antagonist of Wnt8 signaling. Regulates chondrocyte maturation and long bone development By similarity.By similarity

    GO - Molecular functioni

    1. PDZ domain binding Source: RefGenome
    2. protein binding Source: UniProtKB
    3. Wnt-activated receptor activity Source: RefGenome
    4. Wnt-protein binding Source: UniProtKB

    GO - Biological processi

    1. brain development Source: RefGenome
    2. cochlea morphogenesis Source: MGI
    3. convergent extension involved in organogenesis Source: MGI
    4. gonad development Source: RefGenome
    5. inner ear morphogenesis Source: MGI
    6. mammary gland involution Source: RefGenome
    7. negative regulation of canonical Wnt signaling pathway Source: MGI
    8. negative regulation of cartilage development Source: MGI
    9. negative regulation of cell development Source: MGI
    10. negative regulation of cell growth Source: Ensembl
    11. negative regulation of cell proliferation Source: Ensembl
    12. negative regulation of hepatocyte differentiation Source: MGI
    13. negative regulation of Wnt signaling pathway Source: UniProtKB
    14. neural crest cell differentiation Source: BHF-UCL
    15. positive regulation of apoptotic process Source: Ensembl
    16. positive regulation of fat cell differentiation Source: MGI
    17. regulation of transcription from RNA polymerase II promoter Source: RefGenome
    18. somite development Source: BHF-UCL
    19. vasculature development Source: RefGenome
    20. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Wnt signaling pathway

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Secreted frizzled-related protein 3
    Short name:
    sFRP-3
    Alternative name(s):
    Frezzled
    Fritz
    Frizzled-related protein 1
    FrzB-1
    Gene namesi
    Name:Frzb
    Synonyms:Fiz, Fre, Frzb1, Sfrp3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:892032. Frzb.

    Subcellular locationi

    Secreted Curated

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. extracellular region Source: UniProtKB
    3. extracellular space Source: RefGenome

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 3232Sequence AnalysisAdd
    BLAST
    Chaini33 – 323291Secreted frizzled-related protein 3PRO_0000032547Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi35 ↔ 961 Publication
    Disulfide bondi43 ↔ 891 Publication
    Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi80 ↔ 1191 Publication
    Disulfide bondi108 ↔ 1471 Publication
    Disulfide bondi112 ↔ 1361 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP97401.

    PTM databases

    PhosphoSiteiP97401.

    Expressioni

    Tissue specificityi

    Expressed in kidney, brain, testis. Weak expression in spleen and heart.2 Publications

    Developmental stagei

    In early gastrulation, expressed in all three germ layers. In later embryogenesis, expressed in a range of tissues including the central and peripheral nervous systems and the nephogenic mesenchyme.

    Gene expression databases

    BgeeiP97401.
    CleanExiMM_FRZB.
    GenevestigatoriP97401.

    Interactioni

    Subunit structurei

    Interacts with MYOC.1 Publication

    Protein-protein interaction databases

    BioGridi203186. 2 interactions.

    Structurei

    Secondary structure

    1
    323
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi41 – 455
    Helixi62 – 709
    Helixi73 – 764
    Turni77 – 793
    Helixi84 – 929
    Beta strandi97 – 993
    Beta strandi101 – 1033
    Helixi109 – 12517
    Helixi132 – 1343
    Helixi136 – 1383
    Turni142 – 1443
    Beta strandi146 – 1505

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1IJXX-ray1.90A/B/C/D/E/F33-157[»]
    ProteinModelPortaliP97401.
    SMRiP97401. Positions 33-157.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP97401.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini33 – 150118FZPROSITE-ProRule annotationAdd
    BLAST
    Domaini178 – 298121NTRPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi301 – 31818Ser/Thr-richAdd
    BLAST

    Domaini

    The FZ domain is involved in binding with Wnt ligands.By similarity

    Sequence similaritiesi

    Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation
    Contains 1 NTR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG304384.
    GeneTreeiENSGT00750000117492.
    HOGENOMiHOG000231879.
    HOVERGENiHBG070536.
    InParanoidiP97401.
    OMAiEEYLIMG.
    OrthoDBiEOG79PJPC.
    PhylomeDBiP97401.

    Family and domain databases

    Gene3Di1.10.2000.10. 1 hit.
    InterProiIPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR001134. Netrin_domain.
    IPR018933. Netrin_module_non-TIMP.
    IPR026556. SFRP3.
    IPR008993. TIMP-like_OB-fold.
    [Graphical view]
    PANTHERiPTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF76. PTHR11309:SF76. 1 hit.
    PfamiPF01392. Fz. 1 hit.
    PF01759. NTR. 1 hit.
    [Graphical view]
    SMARTiSM00643. C345C. 1 hit.
    SM00063. FRI. 1 hit.
    [Graphical view]
    SUPFAMiSSF50242. SSF50242. 1 hit.
    SSF63501. SSF63501. 1 hit.
    PROSITEiPS50038. FZ. 1 hit.
    PS50189. NTR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97401-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVCCGPGRML LGWAGLLVLA ALCLLQVPGA QAAACEPVRI PLCKSLPWNM    50
    TKMPNHLHHS TQANAILAME QFEGLLGTHC SPDLLFFLCA MYAPICTIDF 100
    QHEPIKPCKS VCERARQGCE PILIKYRHSW PESLACDELP VYDRGVCISP 150
    EAIVTADGAD FPMDSSTGHC RGASSERCKC KPVRATQKTY FRNNYNYVIR 200
    AKVKEVKMKC HDVTAVVEVK EILKASLVNI PRDTVNLYTT SGCLCPPLTV 250
    NEEYVIMGYE DEERSRLLLV EGSIAEKWKD RLGKKVKRWD MKLRHLGLGK 300
    TDASDSTQNQ KSGRNSNPRP ARS 323
    Length:323
    Mass (Da):36,011
    Last modified:May 1, 1997 - v1
    Checksum:i3F1456F8CFC97740
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti321 – 3211A → V in AAH16884. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U68058 mRNA. Translation: AAC53135.1.
    U91905 mRNA. Translation: AAB51300.1.
    U88568 mRNA. Translation: AAC53147.1.
    AK019093 mRNA. Translation: BAB31542.1.
    AK029941 mRNA. Translation: BAC26690.1.
    BC016884 mRNA. Translation: AAH16884.1.
    CCDSiCCDS16176.1.
    RefSeqiNP_035486.1. NM_011356.4.
    UniGeneiMm.427436.

    Genome annotation databases

    EnsembliENSMUST00000028389; ENSMUSP00000028389; ENSMUSG00000027004.
    GeneIDi20378.
    KEGGimmu:20378.
    UCSCiuc008khl.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U68058 mRNA. Translation: AAC53135.1 .
    U91905 mRNA. Translation: AAB51300.1 .
    U88568 mRNA. Translation: AAC53147.1 .
    AK019093 mRNA. Translation: BAB31542.1 .
    AK029941 mRNA. Translation: BAC26690.1 .
    BC016884 mRNA. Translation: AAH16884.1 .
    CCDSi CCDS16176.1.
    RefSeqi NP_035486.1. NM_011356.4.
    UniGenei Mm.427436.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1IJX X-ray 1.90 A/B/C/D/E/F 33-157 [» ]
    ProteinModelPortali P97401.
    SMRi P97401. Positions 33-157.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203186. 2 interactions.

    PTM databases

    PhosphoSitei P97401.

    Proteomic databases

    PRIDEi P97401.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028389 ; ENSMUSP00000028389 ; ENSMUSG00000027004 .
    GeneIDi 20378.
    KEGGi mmu:20378.
    UCSCi uc008khl.1. mouse.

    Organism-specific databases

    CTDi 2487.
    MGIi MGI:892032. Frzb.

    Phylogenomic databases

    eggNOGi NOG304384.
    GeneTreei ENSGT00750000117492.
    HOGENOMi HOG000231879.
    HOVERGENi HBG070536.
    InParanoidi P97401.
    OMAi EEYLIMG.
    OrthoDBi EOG79PJPC.
    PhylomeDBi P97401.

    Miscellaneous databases

    ChiTaRSi FRZB. mouse.
    EvolutionaryTracei P97401.
    NextBioi 298290.
    PROi P97401.
    SOURCEi Search...

    Gene expression databases

    Bgeei P97401.
    CleanExi MM_FRZB.
    Genevestigatori P97401.

    Family and domain databases

    Gene3Di 1.10.2000.10. 1 hit.
    InterProi IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR001134. Netrin_domain.
    IPR018933. Netrin_module_non-TIMP.
    IPR026556. SFRP3.
    IPR008993. TIMP-like_OB-fold.
    [Graphical view ]
    PANTHERi PTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF76. PTHR11309:SF76. 1 hit.
    Pfami PF01392. Fz. 1 hit.
    PF01759. NTR. 1 hit.
    [Graphical view ]
    SMARTi SM00643. C345C. 1 hit.
    SM00063. FRI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50242. SSF50242. 1 hit.
    SSF63501. SSF63501. 1 hit.
    PROSITEi PS50038. FZ. 1 hit.
    PS50189. NTR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Frzb-1 is a secreted antagonist of Wnt signaling expressed in the Spemann organizer."
      Leyns L., Bouwmeester T., Kim S.-H., Piccolo S., de Robertis E.M.
      Cell 88:747-756(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Fritz: a secreted frizzled-related protein that inhibits Wnt activity."
      Mayr T., Deutsch U., Kuehl M., Drexler H.C.A., Lottspeich F., Deutzmann R., Wedlich D., Risau W.
      Mech. Dev. 63:109-125(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
      Tissue: Embryo.
    3. "A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors."
      Rattner A., Hsieh J.-C., Smallwood P.M., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J.
      Proc. Natl. Acad. Sci. U.S.A. 94:2859-2863(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    4. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Tongue.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    6. Cited for: INTERACTION WITH MYOC.
    7. "Insights into Wnt binding and signalling from the structures of two Frizzled cysteine-rich domains."
      Dann C.E., Hsieh J.-C., Rattner A., Sharma D., Nathans J., Leahy D.J.
      Nature 412:86-90(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 33-157, DISULFIDE BONDS.

    Entry informationi

    Entry nameiSFRP3_MOUSE
    AccessioniPrimary (citable) accession number: P97401
    Secondary accession number(s): O09075, O09093, Q91W58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 1, 2001
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 135 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3