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Protein

Calcium-dependent phospholipase A2

Gene

Pla2g5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes L-alpha-palmitoyl-2-oleoyl phosphatidylcholine more efficiently than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol (By similarity).By similarity

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471Calcium; via carbonyl oxygenBy similarity
Metal bindingi49 – 491Calcium; via carbonyl oxygenBy similarity
Metal bindingi51 – 511Calcium; via carbonyl oxygenBy similarity
Active sitei67 – 671By similarity
Metal bindingi68 – 681CalciumBy similarity
Active sitei111 – 1111By similarity

GO - Molecular functioni

  • calcium ion binding Source: InterPro
  • phospholipase A2 activity Source: UniProtKB
  • receptor binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcium-dependent phospholipase A2 (EC:3.1.1.4)
Alternative name(s):
Group V phospholipase A2
PLA2-10
Phosphatidylcholine 2-acylhydrolase 5
Gene namesi
Name:Pla2g5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:101899. Pla2g5.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: MGI
  • extracellular region Source: UniProtKB-SubCell
  • membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 137117Calcium-dependent phospholipase A2PRO_0000022762Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi46 ↔ 137By similarity
Disulfide bondi48 ↔ 64By similarity
Disulfide bondi63 ↔ 117By similarity
Disulfide bondi70 ↔ 110By similarity
Disulfide bondi79 ↔ 103By similarity
Disulfide bondi97 ↔ 108By similarity

Post-translational modificationi

This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP97391.

Expressioni

Gene expression databases

CleanExiMM_PLA2G5.
GenevisibleiP97391. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030524.

Structurei

3D structure databases

ProteinModelPortaliP97391.
SMRiP97391. Positions 22-126.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phospholipase A2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG330703.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiP97391.
KOiK01047.
PhylomeDBiP97391.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKGLLTLAWF LACSVPAVPG GLLELKSMIE KVTGKNAFKN YGFYGCYCGW
60 70 80 90 100
GGRGTPKDGT DWCCQMHDRC YGQLEEKDCA IRTQSYDYRY TNGLVICEHD
110 120 130
SFCPMRLCAC DRKLVYCLRR NLWTYNPLYQ YYPNFLC
Length:137
Mass (Da):15,859
Last modified:December 5, 2001 - v2
Checksum:iF08920E1FD1D912A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 341G → R in AAC53038 (PubMed:8838795).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66873 mRNA. Translation: AAC53038.1.
AF162713 mRNA. Translation: AAD45807.1.
CCDSiCCDS18834.1.
RefSeqiNP_001116426.1. NM_001122954.1.
NP_035240.3. NM_011110.4.
XP_006538721.2. XM_006538658.2.
UniGeneiMm.486532.

Genome annotation databases

GeneIDi18784.
KEGGimmu:18784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U66873 mRNA. Translation: AAC53038.1.
AF162713 mRNA. Translation: AAD45807.1.
CCDSiCCDS18834.1.
RefSeqiNP_001116426.1. NM_001122954.1.
NP_035240.3. NM_011110.4.
XP_006538721.2. XM_006538658.2.
UniGeneiMm.486532.

3D structure databases

ProteinModelPortaliP97391.
SMRiP97391. Positions 22-126.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000030524.

Chemistry

BindingDBiP97391.
ChEMBLiCHEMBL4167.

Proteomic databases

PRIDEiP97391.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18784.
KEGGimmu:18784.

Organism-specific databases

CTDi5322.
MGIiMGI:101899. Pla2g5.

Phylogenomic databases

eggNOGiNOG330703.
HOGENOMiHOG000231749.
HOVERGENiHBG008137.
InParanoidiP97391.
KOiK01047.
PhylomeDBiP97391.

Miscellaneous databases

ChiTaRSiPla2g5. mouse.
NextBioi295048.
PROiP97391.
SOURCEiSearch...

Gene expression databases

CleanExiMM_PLA2G5.
GenevisibleiP97391. MM.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Low-molecular-weight, calcium-dependent phospholipase A2 genes are linked and map to homologous chromosome regions in mouse and human."
    Tischfield J.A., Xia Y.R., Shih D.M., Klisak I., Chen J., Engle S.J., Siakotos A.N., Winstead M.V., Seilhamer J.J., Allamand V., Gyapay G., Lusis A.
    Genomics 32:328-333(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: 129.
  2. "Low molecular weight group IIA and group V phospholipase A(2) enzymes have different intracellular locations in mouse bone marrow-derived mast cells."
    Bingham C.O. III, Fijneman R.J.A., Friend D.S., Goddeau R.P., Rogers R.A., Austen K.F., Arm J.P.
    J. Biol. Chem. 274:31476-31484(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
    Strain: BALB/c.

Entry informationi

Entry nameiPA2G5_MOUSE
AccessioniPrimary (citable) accession number: P97391
Secondary accession number(s): Q9QZU6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 5, 2001
Last modified: June 24, 2015
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.