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P97391 (PA2G5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Calcium-dependent phospholipase A2

EC=3.1.1.4
Alternative name(s):
Group V phospholipase A2
PLA2-10
Phosphatidylcholine 2-acylhydrolase 5
Gene names
Name:Pla2g5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length137 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This isozyme hydrolyzes L-alpha-palmitoyl-2-oleoyl phosphatidylcholine more efficiently than L-alpha-1-palmitoyl-2-arachidonyl phosphatidylcholine, L-alpha-1-palmitoyl-2-arachidonyl phosphatidylethanolamine or L-alpha-1-stearoyl-2-arachidonyl phosphatidylinositol By similarity.

Catalytic activity

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secreted. Membrane. Note: Also associated with various membranous organelles including the Golgi apparatus, nuclear envelope, and plasma membrane. Ref.2

Post-translational modification

This enzyme lacks one of the seven disulfide bonds found in similar PA2 proteins.

Sequence similarities

Belongs to the phospholipase A2 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Chain21 – 137117Calcium-dependent phospholipase A2
PRO_0000022762

Sites

Active site671 By similarity
Active site1111 By similarity
Metal binding471Calcium; via carbonyl oxygen By similarity
Metal binding491Calcium; via carbonyl oxygen By similarity
Metal binding511Calcium; via carbonyl oxygen By similarity
Metal binding681Calcium By similarity

Amino acid modifications

Disulfide bond46 ↔ 137 By similarity
Disulfide bond48 ↔ 64 By similarity
Disulfide bond63 ↔ 117 By similarity
Disulfide bond70 ↔ 110 By similarity
Disulfide bond79 ↔ 103 By similarity
Disulfide bond97 ↔ 108 By similarity

Experimental info

Sequence conflict341G → R in AAC53038. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P97391 [UniParc].

Last modified December 5, 2001. Version 2.
Checksum: F08920E1FD1D912A

FASTA13715,859
        10         20         30         40         50         60 
MKGLLTLAWF LACSVPAVPG GLLELKSMIE KVTGKNAFKN YGFYGCYCGW GGRGTPKDGT 

        70         80         90        100        110        120 
DWCCQMHDRC YGQLEEKDCA IRTQSYDYRY TNGLVICEHD SFCPMRLCAC DRKLVYCLRR 

       130 
NLWTYNPLYQ YYPNFLC 

« Hide

References

[1]"Low-molecular-weight, calcium-dependent phospholipase A2 genes are linked and map to homologous chromosome regions in mouse and human."
Tischfield J.A., Xia Y.R., Shih D.M., Klisak I., Chen J., Engle S.J., Siakotos A.N., Winstead M.V., Seilhamer J.J., Allamand V., Gyapay G., Lusis A.
Genomics 32:328-333(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129.
[2]"Low molecular weight group IIA and group V phospholipase A(2) enzymes have different intracellular locations in mouse bone marrow-derived mast cells."
Bingham C.O. III, Fijneman R.J.A., Friend D.S., Goddeau R.P., Rogers R.A., Austen K.F., Arm J.P.
J. Biol. Chem. 274:31476-31484(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION.
Strain: BALB/c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U66873 mRNA. Translation: AAC53038.1.
AF162713 mRNA. Translation: AAD45807.1.
CCDSCCDS18834.1.
RefSeqNP_001116426.1. NM_001122954.1.
NP_035240.3. NM_011110.4.
XP_006538722.1. XM_006538659.1.
XP_006538723.1. XM_006538660.1.
XP_006538724.1. XM_006538661.1.
UniGeneMm.486532.

3D structure databases

ProteinModelPortalP97391.
SMRP97391. Positions 22-126.
ModBaseSearch...
MobiDBSearch...

Chemistry

BindingDBP97391.
ChEMBLCHEMBL4167.

Proteomic databases

PRIDEP97391.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID18784.
KEGGmmu:18784.

Organism-specific databases

CTD5322.
MGIMGI:101899. Pla2g5.

Phylogenomic databases

eggNOGNOG330703.
HOGENOMHOG000231749.
HOVERGENHBG008137.
KOK01047.
PhylomeDBP97391.

Gene expression databases

CleanExMM_PLA2G5.
GenevestigatorP97391.

Family and domain databases

Gene3D1.20.90.10. 1 hit.
InterProIPR001211. PLipase_A2.
IPR013090. PLipase_A2_AS.
IPR016090. PLipase_A2_dom.
[Graphical view]
PANTHERPTHR11716. PTHR11716. 1 hit.
PfamPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSPR00389. PHPHLIPASEA2.
SMARTSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMSSF48619. SSF48619. 1 hit.
PROSITEPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPLA2G5. mouse.
NextBio295048.
PROP97391.
SOURCESearch...

Entry information

Entry namePA2G5_MOUSE
AccessionPrimary (citable) accession number: P97391
Secondary accession number(s): Q9QZU6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: December 5, 2001
Last modified: July 9, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot