Reviewed,
UniProtKB/Swiss-Prot P97386 (DNLI3_MOUSE)
Last modified
June 16, 2009.
Version 82.
History...
Clusters with 100%,
90%,
50% identity |
 Documents (2) |
 Third-party data |
 Customize display | text xml rdf/xml gff fasta |
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: DNA ligase 3 EC=6.5.1.1 Alternative name(s): DNA ligase III Polydeoxyribonucleotide synthase [ATP] 3 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 1015 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | The alpha isoform interacts with DNA-repair protein XRCC1 and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. The beta isoform does not interact with XRCC1 and may be specifically involved in the completion of homologous recombination events that occur during meiotic prophase. |
| Catalytic activity | ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). |
| Cofactor | Magnesium By similarity. |
| Subcellular location | |
| Tissue specificity | The alpha isoform is expressed in all tissues, while the beta isoform is expressed only in the testis. |
| Developmental stage | During male germ cell differentiation, expression of the beta isoform begins in the later stages of meiotic prophase and ends in the round spermatid stage. |
| Sequence similarities | Belongs to the ATP-dependent DNA ligase family. Contains 1 BRCT domain. Contains 1 PARP-type zinc finger. |
Ontologies
Alternative products
| This entry describes 2 isoforms produced by alternative splicing. [Align] [Select] | ||||||
| Isoform Alpha (identifier: P97386-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform Beta (identifier: P97386-2) The sequence of this isoform differs from the canonical sequence as follows: 939-1015: VLLDVFTGVR...IRKRRLIAPC → RRRASRQRGRKAMQTGRR |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1015 | 1015 | DNA ligase 3 | PRO_0000059575 | |||||
Regions | |||||||||
| Domain | 939 – 1015 | 77 | BRCT | ||||||
| Zinc finger | 94 – 186 | 93 | PARP-type | ||||||
Sites | |||||||||
| Active site | 512 | 1 | N6-AMP-lysine intermediate By similarity | ||||||
| Metal binding | 564 | 1 | Magnesium 1 By similarity | ||||||
| Metal binding | 659 | 1 | Magnesium 2 By similarity | ||||||
| Binding site | 510 | 1 | ATP By similarity | ||||||
| Binding site | 517 | 1 | ATP By similarity | ||||||
| Binding site | 532 | 1 | ATP By similarity | ||||||
| Binding site | 664 | 1 | ATP By similarity | ||||||
| Binding site | 675 | 1 | ATP By similarity | ||||||
| Binding site | 679 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 210 | 1 | Phosphothreonine Ref.2 | ||||||
| Modified residue | 211 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 228 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 244 | 1 | Phosphoserine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 939 – 1015 | 77 | VLLDV…LIAPC → RRRASRQRGRKAMQTGRR in isoform Beta. | VSP_001303 | |||||
Sequences
| ||||||||||||||||||||||||
References
| « Hide 'large scale' references | |
| [1] | "An alternative splicing event which occurs in mouse pachytene spermatocytes generates a form of DNA ligase III with distinct biochemical properties that may function in meiotic recombination." Mackey Z.B., Ramos W., Levin D.S., Walter C.A., McCarrey J.R., Tomkinson A.E. Mol. Cell. Biol. 17:989-998(1997) [PubMed: 9001252] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). Tissue: Testis. |
| [2] | "Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-210, MASS SPECTROMETRY. Tissue: Liver. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| U66058 mRNA. Translation: AAC53004.1. U66057 mRNA. Translation: AAC53003.1. | |
| IPI | IPI00124272. IPI00230471. |
| UniGene | Mm.277136 Mm.413071 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1IMO based on UniProtKB P49916. |
| SMR | P97386. Positions 89-205. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P97386. |
Proteomic databases | |
| PRIDE | P97386. |
Genome annotation databases | |
| Ensembl | ENSMUSG00000020697. Mus musculus. [Contig view] |
Organism-specific databases | |
| MGI | MGI:109152. Lig3. |
Phylogenomic databases | |
| HOGENOM | P97386. |
| HOVERGEN | P97386. |
Enzyme and pathway databases | |
| BRENDA | 6.5.1.1. 244. |
Gene expression databases | |
| ArrayExpress | P97386. |
| Bgee | P97386. |
| CleanEx | MM_LIG3. |
| GermOnline | ENSMUSG00000020697. Mus musculus. |
Family and domain databases | |
| InterPro | IPR001357. BRCT. IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. IPR001510. Znf_PARP. [Graphical view] |
| Gene3D | G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:3.30.1740.10. Znf_PARP. 1 hit. |
| Pfam | PF00533. BRCT. 1 hit. PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. PF00645. zf-PARP. 1 hit. [Graphical view] |
| ProDom | PD004675. Znf_PolyADPpol. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00292. BRCT. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR00574. dnl1. 1 hit. |
| PROSITE | PS50172. BRCT. 1 hit. PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. PS00347. PARP_ZN_FINGER_1. 1 hit. PS50064. PARP_ZN_FINGER_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| SOURCE | Search... |
Entry information
| Entry name | DNLI3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P97386 Secondary accession number(s): P97385 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |
