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Protein

Interleukin-12 receptor subunit beta-2

Gene

Il12rb2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for interleukin-12. This subunit is the signaling component coupling to the JAK2/STAT4 pathway. Promotes the proliferation of T-cells as well as NK cells. Induces the promotion of T-cells towards the Th1 phenotype by strongly enhancing IFN-gamma production. Can also activate STAT3.1 Publication

GO - Molecular functioni

  • cytokine receptor activity Source: InterPro
  • protein kinase binding Source: MGI

GO - Biological processi

  • interferon-gamma production Source: MGI
  • peptidyl-tyrosine phosphorylation Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • response to cytokine Source: MGI
  • response to lipopolysaccharide Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiR-MMU-447115. Interleukin-12 signaling.
R-MMU-6788467. IL-6-type cytokine receptor ligand interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-12 receptor subunit beta-2
Short name:
IL-12 receptor subunit beta-2
Short name:
IL-12R subunit beta-2
Short name:
IL-12R-beta-2
Short name:
IL-12RB2
Gene namesi
Name:Il12rb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1270861. Il12rb2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 637ExtracellularSequence analysisAdd BLAST614
Transmembranei638 – 658HelicalSequence analysisAdd BLAST21
Topological domaini659 – 874CytoplasmicSequence analysisAdd BLAST216

GO - Cellular componenti

  • external side of plasma membrane Source: MGI
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi677Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1
Mutagenesisi693Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1
Mutagenesisi727Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1
Mutagenesisi737Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1
Mutagenesisi748Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1
Mutagenesisi757Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1
Mutagenesisi778Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1
Mutagenesisi804Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1
Mutagenesisi811Y → F: No loss of IL12-induced STAT4 activation. Greatly reduced IL12-induced T-cell proliferation and IFN-gamma production. 1 Publication1
Mutagenesisi866Y → F: No loss of IL12-induced STAT4 activation nor T-cell proliferation. No effect on IFN-gamma production. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001092124 – 874Interleukin-12 receptor subunit beta-2Add BLAST851

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi48N-linked (GlcNAc...)Sequence analysis1
Glycosylationi101N-linked (GlcNAc...)Sequence analysis1
Glycosylationi114N-linked (GlcNAc...)Sequence analysis1
Glycosylationi142N-linked (GlcNAc...)Sequence analysis1
Glycosylationi151N-linked (GlcNAc...)Sequence analysis1
Glycosylationi169N-linked (GlcNAc...)Sequence analysis1
Glycosylationi179N-linked (GlcNAc...)Sequence analysis1
Glycosylationi224N-linked (GlcNAc...)Sequence analysis1
Glycosylationi252N-linked (GlcNAc...)Sequence analysis1
Glycosylationi279N-linked (GlcNAc...)Sequence analysis1
Glycosylationi287N-linked (GlcNAc...)Sequence analysis1
Glycosylationi323N-linked (GlcNAc...)Sequence analysis1
Glycosylationi391N-linked (GlcNAc...)Sequence analysis1
Glycosylationi495N-linked (GlcNAc...)Sequence analysis1
Modified residuei757PhosphotyrosineCurated1
Modified residuei804PhosphotyrosineCurated1
Modified residuei811PhosphotyrosineCurated1

Post-translational modificationi

On IL12 stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation of any one of Tyr-757, Tyr-804 or Tyr-811 can activate STAT4, IFN-gamma production, and T-cell proliferation. Tyr-811 is the dominant site of cell proliferation.

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP97378.
PRIDEiP97378.

PTM databases

PhosphoSitePlusiP97378.

Expressioni

Tissue specificityi

Expressed in developing T-helper (TH) cells.

Developmental stagei

Expressed at high levels in Th1 cells on day 3, 5 and 7 after primary activation. Very low expression in Th2 cells on day 3 and not detectable on day 5 nor day 7 after activation.1 Publication

Inductioni

Following T-cell activation, expression inhibited by IL4 and induced by IFN gamma.

Gene expression databases

BgeeiENSMUSG00000018341.
CleanExiMM_IL12RB2.
ExpressionAtlasiP97378. baseline and differential.
GenevisibleiP97378. MM.

Interactioni

Subunit structurei

Heterodimer/heterooligomer; disulfide-linked. The functional high affinity IL12 receptor is composed of I12RB1 and IL12RB2. Il12RB2 binds JAK2 (via its N-terminal) through a membrane-proximal region of the cytoplasmic domain (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GakQ99KY47EBI-6253448,EBI-7652906
Il12rb1Q608372EBI-6253448,EBI-2481455
Il6stQ005602EBI-6253448,EBI-3862992

GO - Molecular functioni

  • protein kinase binding Source: MGI

Protein-protein interaction databases

BioGridi200612. 1 interactor.
IntActiP97378. 3 interactors.
MINTiMINT-6504587.
STRINGi10090.ENSMUSP00000010605.

Structurei

3D structure databases

ProteinModelPortaliP97378.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini139 – 237Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST99
Domaini242 – 335Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST94
Domaini336 – 430Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST95
Domaini438 – 530Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST93
Domaini536 – 635Fibronectin type-III 5PROSITE-ProRule annotationAdd BLAST100

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi321 – 325WSXWS motif5
Motifi677 – 685Box 1 motif9

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 5 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHXG. Eukaryota.
ENOG41116V2. LUCA.
GeneTreeiENSGT00550000074436.
HOGENOMiHOG000049086.
HOVERGENiHBG052059.
InParanoidiP97378.
KOiK05064.
OMAiCILHYRI.
OrthoDBiEOG091G01XM.
PhylomeDBiP97378.
TreeFamiTF338122.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 5 hits.
InterProiIPR003961. FN3_dom.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 4 hits.
PROSITEiPS50853. FN3. 4 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97378-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQTVRECSL ALLFLFMWLL IKANIDVCKL GTVTVQPAPV IPLGSAANIS
60 70 80 90 100
CSLNPKQGCS HYPSSNELIL LKFVNDVLVE NLHGKKVHDH TGHSSTFQVT
110 120 130 140 150
NLSLGMTLFV CKLNCSNSQK KPPVPVCGVE ISVGVAPEPP QNISCVQEGE
160 170 180 190 200
NGTVACSWNS GKVTYLKTNY TLQLSGPNNL TCQKQCFSDN RQNCNRLDLG
210 220 230 240 250
INLSPDLAES RFIVRVTAIN DLGNSSSLPH TFTFLDIVIP LPPWDIRINF
260 270 280 290 300
LNASGSRGTL QWEDEGQVVL NQLRYQPLNS TSWNMVNATN AKGKYDLRDL
310 320 330 340 350
RPFTEYEFQI SSKLHLSGGS WSNWSESLRT RTPEEEPVGI LDIWYMKQDI
360 370 380 390 400
DYDRQQISLF WKSLNPSEAR GKILHYQVTL QEVTKKTTLQ NTTRHTSWTR
410 420 430 440 450
VIPRTGAWTA SVSAANSKGA SAPTHINIVD LCGTGLLAPH QVSAKSENMD
460 470 480 490 500
NILVTWQPPK KADSAVREYI VEWRALQPGS ITKFPPHWLR IPPDNMSALI
510 520 530 540 550
SENIKPYICY EIRVHALSES QGGCSSIRGD SKHKAPVSGP HITAITEKKE
560 570 580 590 600
RLFISWTHIP FPEQRGCILH YRIYWKERDS TAQPELCEIQ YRRSQNSHPI
610 620 630 640 650
SSLQPRVTYV LWMTAVTAAG ESPQGNEREF CPQGKANWKA FVISSICIAI
660 670 680 690 700
ITVGTFSIRY FRQKAFTLLS TLKPQWYSRT IPDPANSTWV KKYPILEEKI
710 720 730 740 750
QLPTDNLLMA WPTPEEPEPL IIHEVLYHMI PVVRQPYYFK RGQGFQGYST
760 770 780 790 800
SKQDAMYIAN PQATGTLTAE TRQLVNLYKV LESRDPDSKL ANLTSPLTVT
810 820 830 840 850
PVNYLPSHEG YLPSNIEDLS PHEADPTDSF DLEHQHISLS IFASSSLRPL
860 870
IFGGERLTLD RLKMGYDSLM SNEA
Length:874
Mass (Da):98,197
Last modified:May 1, 1997 - v1
Checksum:i582E4D21BF1FBD67
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64199 mRNA. Translation: AAB36676.1.
BC137745 mRNA. Translation: AAI37746.1.
CCDSiCCDS20220.1.
RefSeqiNP_001298070.1. NM_001311141.1.
NP_032380.1. NM_008354.3.
XP_006505680.1. XM_006505617.3.
UniGeneiMm.188337.

Genome annotation databases

EnsembliENSMUST00000018485; ENSMUSP00000010605; ENSMUSG00000018341.
GeneIDi16162.
KEGGimmu:16162.
UCSCiuc009cfo.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U64199 mRNA. Translation: AAB36676.1.
BC137745 mRNA. Translation: AAI37746.1.
CCDSiCCDS20220.1.
RefSeqiNP_001298070.1. NM_001311141.1.
NP_032380.1. NM_008354.3.
XP_006505680.1. XM_006505617.3.
UniGeneiMm.188337.

3D structure databases

ProteinModelPortaliP97378.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200612. 1 interactor.
IntActiP97378. 3 interactors.
MINTiMINT-6504587.
STRINGi10090.ENSMUSP00000010605.

PTM databases

PhosphoSitePlusiP97378.

Proteomic databases

PaxDbiP97378.
PRIDEiP97378.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018485; ENSMUSP00000010605; ENSMUSG00000018341.
GeneIDi16162.
KEGGimmu:16162.
UCSCiuc009cfo.1. mouse.

Organism-specific databases

CTDi3595.
MGIiMGI:1270861. Il12rb2.

Phylogenomic databases

eggNOGiENOG410IHXG. Eukaryota.
ENOG41116V2. LUCA.
GeneTreeiENSGT00550000074436.
HOGENOMiHOG000049086.
HOVERGENiHBG052059.
InParanoidiP97378.
KOiK05064.
OMAiCILHYRI.
OrthoDBiEOG091G01XM.
PhylomeDBiP97378.
TreeFamiTF338122.

Enzyme and pathway databases

ReactomeiR-MMU-447115. Interleukin-12 signaling.
R-MMU-6788467. IL-6-type cytokine receptor ligand interactions.

Miscellaneous databases

ChiTaRSiIl12rb2. mouse.
PROiP97378.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018341.
CleanExiMM_IL12RB2.
ExpressionAtlasiP97378. baseline and differential.
GenevisibleiP97378. MM.

Family and domain databases

CDDicd00063. FN3. 3 hits.
Gene3Di2.60.40.10. 5 hits.
InterProiIPR003961. FN3_dom.
IPR003529. Hematopoietin_rcpt_Gp130_CS.
IPR013783. Ig-like_fold.
IPR010457. IgC2-like_lig-bd.
[Graphical view]
PfamiPF00041. fn3. 3 hits.
PF06328. Lep_receptor_Ig. 1 hit.
[Graphical view]
SMARTiSM00060. FN3. 4 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 4 hits.
PROSITEiPS50853. FN3. 4 hits.
PS01353. HEMATOPO_REC_L_F2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiI12R2_MOUSE
AccessioniPrimary (citable) accession number: P97378
Secondary accession number(s): B9EHD9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: November 30, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Lps-defective mice C57BL/10ScCr (Cr) mice carry a mutation in the IL12RB2 gene leading to the production of a truncated IL12 receptor beta 2 chain resulting in malfunction of the IL12-mediated IFN-gamma response.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.