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Reviewed, UniProtKB/Swiss-Prot P97377 (CDK2_MOUSE)

Last modified June 16, 2009. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Cell division protein kinase 2
    EC=2.7.11.22
Gene names
Name: Cdk2
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMus

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2 By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-160 activates it By similarity.

Subunit structure

Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1 By similarity. Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily.

Contains 1 protein kinase domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform CDK2-beta (identifier: P97377-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform CDK2-alpha (identifier: P97377-2)

The sequence of this isoform differs from the canonical sequence as follows:
     197-244: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Cell division protein kinase 2
PRO_0000085771

Regions

Domain4 – 334331Protein kinase
Nucleotide binding10 – 189ATP By similarity
Nucleotide binding81 – 833ATP By similarity
Nucleotide binding129 – 1324ATP By similarity

Sites

Active site1271Proton acceptor By similarity
Binding site331ATP By similarity
Binding site861ATP By similarity
Binding site1451ATP By similarity

Amino acid modifications

Modified residue141Phosphothreonine By similarity
Modified residue151Phosphotyrosine; by WEE1 Ref.4
Modified residue191Phosphotyrosine By similarity
Modified residue461Phosphoserine By similarity
Modified residue1601Phosphothreonine; by CAK and CCRK By similarity

Natural variations

Alternative sequence197 – 24448Missing in isoform CDK2-alpha.
VSP_004800

Experimental info

Mutagenesis151Y → F: Loss of thyrosine phosphorylation by WEE1 and CABLES1. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform CDK2-beta [UniParc].

Last modified May 30, 2000. Version 2.
Checksum: D806BC2F150AEDFC

FASTA34638,978
        10         20         30         40         50         60 
MENFQKVEKI GEGTYGVVYK AKNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH 

        70         80         90        100        110        120 
PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS 

       130        140        150        160        170        180 
HRVLHRDLKP QNLLINAEGS IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY 

       190        200        210        220        230        240 
STAVDIWSLG CIFAEMHLVC TQHHAKCCGE HRRNGRHSLC PLCSYLEVAA SQGGGMTAVS 

       250        260        270        280        290        300 
APHPVTRRAL FPGDSEIDQL FRIFRTLGTP DEVVWPGVTS MPDYKPSFPK WARQDFSKVV 

       310        320        330        340 
PPLDEDGRSL LSQMLHYDPN KRISAKAALA HPFFQDVTKP VPHLRL 

« Hide

Isoform CDK2-alpha.

Checksum: FB28DBD8BA587BDA
Show »

FASTA29833,887

References

« Hide 'large scale' references
[1]"Exon-intron organization of the murine cyclin-dependent kinase-2 genes Cdk2-alpha and Cdk2-beta."
Jun D., Lee Y.H., Park H.K., Kim Y.H.
Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CDK2-ALPHA).
Strain: C57BL/6.
[2]"The 39 kDa form of CDK2 arises through alternative splicing, is expressed in many but not all mammals, and is an active kinase."
Ellenrieder C., Bartosch B., Lee G.Y., Murphy M., Sweeney C., Hergersberg M., Hunt T., Carrington M., Jaussi R.
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CDK2-BETA).
Strain: FVB/N.
Tissue: Mammary gland.
[4]"Cables enhances cdk2 tyrosine 15 phosphorylation by Wee1, inhibits cell growth, and is lost in many human colon and squamous cancers."
Wu C.-L., Kirley S.D., Xiao H., Chuang Y., Chung D.C., Zukerberg L.R.
Cancer Res. 61:7325-7332(2001) [PubMed: 11585773] [Abstract]
Cited for: PHOSPHORYLATION AT TYR-15, MUTAGENESIS OF TYR-15, IDENTIFICATION IN A COMPLEX WITH CABLES1; CCNA1 AND CCNE1, INTERACTION WITH CABLES1.
[5]"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-15, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

U63337 mRNA. Translation: AAB37128.1.
AJ223732 mRNA. Translation: CAA11533.1.
AJ223733 Genomic DNA. Translation: CAA11534.1.
AJ223733 Genomic DNA. Translation: CAA11535.1.
BC005654 mRNA. Translation: AAH05654.1.
IPIIPI00124240.
IPI00225350.
RefSeqNP_904326.1.
UniGeneMm.111326

3D structure databases

HSSPHSSP built from PDB template 1H27 based on UniProtKB P24941.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:24176N.
IntActP97377. 6 interactions.

PTM databases

PhosphoSiteP97377.

Genome annotation databases

EnsemblENSMUSG00000025358. Mus musculus. [Contig view]
GeneID12566.

Organism-specific databases

MGIMGI:104772. Cdk2.

Phylogenomic databases

HOGENOMP97377.
HOVERGENP97377.
OMAP97377. GVPLRAY.

Enzyme and pathway databases

BRENDA2.7.11.22. 244.

Gene expression databases

ArrayExpressP97377.
BgeeP97377.
CleanExMM_CDK2.
GermOnlineENSMUSG00000025358. Mus musculus.

Family and domain databases

InterProIPR000719. Prot_kinase_core.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_pkinase-rel.
IPR008271. Ser_thr_pkin_AS.
IPR002290. Ser_thr_pkinase.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
ProDomPD000001. Prot_kinase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio281656.
SOURCESearch...

Entry information

Entry nameCDK2_MOUSE
AccessionPrimary (citable) accession number: P97377
Secondary accession number(s): O55105
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 30, 2000
Last modified: June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents