Reviewed, UniProtKB/Swiss-Prot P97377 (CDK2_MOUSE)

Names and origin

Protein names

Recommended name:
    Cell division protein kinase 2
    EC=2.7.11.22

Gene names

Name:

Cdk2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	346 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the control of the cell cycle. Interacts with cyclins A, B1, B3, D, or E. Activity of CDK2 is maximal during S phase and G2 By similarity.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation	Phosphorylation at Thr-14 or Tyr-15 inactivates the enzyme, while phosphorylation at Thr-160 activates it By similarity.
Subunit structure	Interacts with UHRF2. Part of a complex consisting of UHRF2, CDK2 and CCNE1. Interacts with the Speedy/Ringo proteins SPDYA and SPDYC. Found in a complex with both SPDYA and CDKN1B/KIP1 By similarity. Found in a complex with CABLES1, CCNA1 and CCNE1. Interacts with CABLES1.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Coding sequence diversity	Alternative splicing
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	cell division Inferred from electronic annotation. Source: UniProtKB-KW mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of transcription Inferred from genetic interaction. Source: MGI potassium ion transport Inferred from genetic interaction. Source: MGI protein amino acid phosphorylation Inferred from direct assay. Source: MGI
Cellular component	X chromosome Inferred from direct assay. Source: MGI Y chromosome Inferred from direct assay. Source: MGI chromosome, telomeric region Inferred from direct assay. Source: MGI condensed chromosome Inferred from direct assay. Source: MGI cyclin-dependent protein kinase holoenzyme complex Inferred from physical interaction. Source: MGI transcription factor complex Inferred from direct assay. Source: MGI
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein kinase activity Inferred from direct assay. Source: MGI protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform CDK2-beta (identifier: P97377-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform CDK2-alpha (identifier: P97377-2)

The sequence of this isoform differs from the canonical sequence as follows:
     197-244: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 346	346	Cell division protein kinase 2		PRO_0000085771
Regions
	Domain	4 – 334	331	Protein kinase
	Nucleotide binding	10 – 18	9	ATP By similarity
	Nucleotide binding	81 – 83	3	ATP By similarity
	Nucleotide binding	129 – 132	4	ATP By similarity
Sites
	Active site	127	1	Proton acceptor By similarity
	Binding site	33	1	ATP By similarity
	Binding site	86	1	ATP By similarity
	Binding site	145	1	ATP By similarity
Amino acid modifications
	Modified residue	14	1	Phosphothreonine By similarity
	Modified residue	15	1	Phosphotyrosine; by WEE1 Ref.4
	Modified residue	19	1	Phosphotyrosine By similarity
	Modified residue	46	1	Phosphoserine By similarity
	Modified residue	160	1	Phosphothreonine; by CAK and CCRK By similarity
Natural variations
	Alternative sequence	197 – 244	48	Missing in isoform CDK2-alpha.		VSP_004800
Experimental info
	Mutagenesis	15	1	Y → F: Loss of thyrosine phosphorylation by WEE1 and CABLES1. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform CDK2-beta [UniParc]. Last modified May 30, 2000. Version 2. Checksum: D806BC2F150AEDFC	FASTA	346	38,978
	10 20 30 40 50 60 MENFQKVEKI GEGTYGVVYK AKNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH 70 80 90 100 110 120 PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS 130 140 150 160 170 180 HRVLHRDLKP QNLLINAEGS IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY 190 200 210 220 230 240 STAVDIWSLG CIFAEMHLVC TQHHAKCCGE HRRNGRHSLC PLCSYLEVAA SQGGGMTAVS 250 260 270 280 290 300 APHPVTRRAL FPGDSEIDQL FRIFRTLGTP DEVVWPGVTS MPDYKPSFPK WARQDFSKVV 310 320 330 340 PPLDEDGRSL LSQMLHYDPN KRISAKAALA HPFFQDVTKP VPHLRL
	Isoform CDK2-alpha. Checksum: FB28DBD8BA587BDA	FASTA	298	33,887
	10 20 30 40 50 60 MENFQKVEKI GEGTYGVVYK AKNKLTGEVV ALKKIRLDTE TEGVPSTAIR EISLLKELNH 70 80 90 100 110 120 PNIVKLLDVI HTENKLYLVF EFLHQDLKKF MDASALTGIP LPLIKSYLFQ LLQGLAFCHS 130 140 150 160 170 180 HRVLHRDLKP QNLLINAEGS IKLADFGLAR AFGVPVRTYT HEVVTLWYRA PEILLGCKYY 190 200 210 220 230 240 STAVDIWSLG CIFAEMVTRR ALFPGDSEID QLFRIFRTLG TPDEVVWPGV TSMPDYKPSF 250 260 270 280 290 PKWARQDFSK VVPPLDEDGR SLLSQMLHYD PNKRISAKAA LAHPFFQDVT KPVPHLRL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Exon-intron organization of the murine cyclin-dependent kinase-2 genes Cdk2-alpha and Cdk2-beta." Jun D., Lee Y.H., Park H.K., Kim Y.H. Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CDK2-ALPHA). Strain: C57BL/6.
[2]	"The 39 kDa form of CDK2 arises through alternative splicing, is expressed in many but not all mammals, and is an active kinase." Ellenrieder C., Bartosch B., Lee G.Y., Murphy M., Sweeney C., Hergersberg M., Hunt T., Carrington M., Jaussi R. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], ALTERNATIVE SPLICING.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM CDK2-BETA). Strain: FVB/N. Tissue: Mammary gland.
[4]	"Cables enhances cdk2 tyrosine 15 phosphorylation by Wee1, inhibits cell growth, and is lost in many human colon and squamous cancers." Wu C.-L., Kirley S.D., Xiao H., Chuang Y., Chung D.C., Zukerberg L.R. Cancer Res. 61:7325-7332(2001) [PubMed: 11585773] [Abstract] Cited for: PHOSPHORYLATION AT TYR-15, MUTAGENESIS OF TYR-15, IDENTIFICATION IN A COMPLEX WITH CABLES1; CCNA1 AND CCNE1, INTERACTION WITH CABLES1.
[5]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-15, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U63337 mRNA. Translation: AAB37128.1. AJ223732 mRNA. Translation: CAA11533.1. AJ223733 Genomic DNA. Translation: CAA11534.1. AJ223733 Genomic DNA. Translation: CAA11535.1. BC005654 mRNA. Translation: AAH05654.1.
IPI	IPI00124240. IPI00225350.
RefSeq	NP_904326.1.
UniGene	Mm.111326
3D structure databases
HSSP	HSSP built from PDB template 1H27 based on UniProtKB P24941.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:24176N.
IntAct	P97377. 6 interactions.
PTM databases
PhosphoSite	P97377.
Genome annotation databases
Ensembl	ENSMUSG00000025358. Mus musculus. [Contig view]
GeneID	12566.
Organism-specific databases
MGI	MGI:104772. Cdk2.
Phylogenomic databases
HOGENOM	P97377.
HOVERGEN	P97377.
OMA	P97377. GVPLRAY.
Enzyme and pathway databases
BRENDA	2.7.11.22. 244.
Gene expression databases
ArrayExpress	P97377.
Bgee	P97377.
CleanEx	MM_CDK2.
GermOnline	ENSMUSG00000025358. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	281656.
SOURCE	Search...

Entry information

Entry name

CDK2_MOUSE

Accession

Primary (citable) accession number: P97377
Secondary accession number(s): O55105

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 30, 2000
Last modified:	June 16, 2009
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)