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Protein

Proteasome activator complex subunit 1

Gene

Psme1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

GO - Molecular functioni

  1. endopeptidase activator activity Source: MGI

GO - Biological processi

  1. antigen processing and presentation of exogenous antigen Source: MGI
  2. positive regulation of endopeptidase activity Source: GOC
Complete GO annotation...

Enzyme and pathway databases

ReactomeiREACT_274287. Degradation of beta-catenin by the destruction complex.
REACT_275686. Activation of NF-kappaB in B cells.
REACT_275732. degradation of AXIN.
REACT_278878. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_289441. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_294625. Ubiquitin-dependent degradation of Cyclin D1.
REACT_297888. degradation of DVL.
REACT_299120. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_301078. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_310780. Degradation of GLI2 by the proteasome.
REACT_315933. Orc1 removal from chromatin.
REACT_321346. Separation of Sister Chromatids.
REACT_324511. Degradation of GLI1 by the proteasome.
REACT_326233. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_329431. APC/C:Cdc20 mediated degradation of Securin.
REACT_331452. Hh ligand biogenesis disease.
REACT_334737. Regulation of ornithine decarboxylase (ODC).
REACT_336463. CDK-mediated phosphorylation and removal of Cdc6.
REACT_336745. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_337766. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_341044. SCF-beta-TrCP mediated degradation of Emi1.
REACT_341075. ER-Phagosome pathway.
REACT_342086. SCF(Skp2)-mediated degradation of p27/p21.
REACT_342636. Asymmetric localization of PCP proteins.
REACT_343561. CDT1 association with the CDC6:ORC:origin complex.
REACT_343568. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_345193. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_347264. Hedgehog ligand biogenesis.
REACT_351311. Hedgehog 'on' state.
REACT_353777. GLI3 is processed to GLI3R by the proteasome.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome activator complex subunit 1
Alternative name(s):
11S regulator complex subunit alpha
Short name:
REG-alpha
Activator of multicatalytic protease subunit 1
Proteasome activator 28 subunit alpha
Short name:
PA28a
Short name:
PA28alpha
Gene namesi
Name:Psme1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1096367. Psme1.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. proteasome activator complex Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Proteasome activator complex subunit 1PRO_0000161781Add
BLAST

Proteomic databases

MaxQBiP97371.
PaxDbiP97371.
PRIDEiP97371.

PTM databases

PhosphoSiteiP97371.

Expressioni

Inductioni

By interferon gamma.

Gene expression databases

BgeeiP97371.
CleanExiMM_PSME1.
ExpressionAtlasiP97371. baseline and differential.
GenevestigatoriP97371.

Interactioni

Subunit structurei

Heterodimer of PSME1 and PSME2, which forms a hexameric ring. PSME1 can form homoheptamers (By similarity).By similarity

Protein-protein interaction databases

BioGridi202432. 1 interaction.
IntActiP97371. 3 interactions.
MINTiMINT-1844401.
STRINGi10090.ENSMUSP00000087046.

Structurei

3D structure databases

SMRiP97371. Positions 4-63, 104-242.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PA28 family.Curated

Phylogenomic databases

eggNOGiNOG265006.
HOGENOMiHOG000282822.
HOVERGENiHBG053745.
InParanoidiP97371.
KOiK06696.
OMAiTQISKYY.
PhylomeDBiP97371.
TreeFamiTF106236.

Family and domain databases

Gene3Di1.20.120.180. 1 hit.
1.20.5.120. 1 hit.
InterProiIPR009077. Proteasome_activ_pa28.
IPR003186. Proteasome_activ_pa28_C.
IPR003185. Proteasome_activ_pa28_N.
[Graphical view]
PANTHERiPTHR10660. PTHR10660. 1 hit.
PfamiPF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
[Graphical view]
SUPFAMiSSF47216. SSF47216. 2 hits.

Sequencei

Sequence statusi: Complete.

P97371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLRVHPEA QAKVDVFRED LCSKTENLLG SYFPKKISEL DAFLKEPALN
60 70 80 90 100
EANLSNLKAP LDIPVPDPVK EKEKEERKKQ QEKEEKEEKK KGDEDDKGPP
110 120 130 140 150
CGPVNCNEKI VVLLQRLKPE IKDVTEQLNL VTTWLQLQIP RIEDGNNFGV
160 170 180 190 200
AVQEKVFELM TNLHTKLEGF HTQISKYFSE RGDAVAKAAK QPHVGDYRQL
210 220 230 240
VHELDEAEYQ EIRLMVMEIR NAYAVLYDII LKNFEKLKKP RGETKGMIY
Length:249
Mass (Da):28,673
Last modified:August 29, 2001 - v2
Checksum:i1E2CE09DA44A5346
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti17 – 171F → L in BAB25712 (PubMed:16141072).Curated
Sequence conflicti97 – 971K → E in BAB25712 (PubMed:16141072).Curated
Sequence conflicti151 – 1511A → S in AAC53295 (PubMed:9162094).Curated
Sequence conflicti156 – 1561V → L in AAC53295 (PubMed:9162094).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87909 mRNA. Translation: BAA22039.1.
U60328 mRNA. Translation: AAC53295.1.
AB007136 Genomic DNA. Translation: BAA28835.1.
AB053120 Genomic DNA. Translation: BAB47403.1.
AK019390 mRNA. Translation: BAB31696.1.
AK008514 mRNA. Translation: BAB25712.1.
CCDSiCCDS36931.1.
RefSeqiNP_035319.1. NM_011189.1.
UniGeneiMm.830.

Genome annotation databases

EnsembliENSMUST00000174259; ENSMUSP00000134735; ENSMUSG00000022216.
GeneIDi19186.
KEGGimmu:19186.
UCSCiuc007tzd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87909 mRNA. Translation: BAA22039.1.
U60328 mRNA. Translation: AAC53295.1.
AB007136 Genomic DNA. Translation: BAA28835.1.
AB053120 Genomic DNA. Translation: BAB47403.1.
AK019390 mRNA. Translation: BAB31696.1.
AK008514 mRNA. Translation: BAB25712.1.
CCDSiCCDS36931.1.
RefSeqiNP_035319.1. NM_011189.1.
UniGeneiMm.830.

3D structure databases

SMRiP97371. Positions 4-63, 104-242.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202432. 1 interaction.
IntActiP97371. 3 interactions.
MINTiMINT-1844401.
STRINGi10090.ENSMUSP00000087046.

PTM databases

PhosphoSiteiP97371.

Proteomic databases

MaxQBiP97371.
PaxDbiP97371.
PRIDEiP97371.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000174259; ENSMUSP00000134735; ENSMUSG00000022216.
GeneIDi19186.
KEGGimmu:19186.
UCSCiuc007tzd.1. mouse.

Organism-specific databases

CTDi5720.
MGIiMGI:1096367. Psme1.

Phylogenomic databases

eggNOGiNOG265006.
HOGENOMiHOG000282822.
HOVERGENiHBG053745.
InParanoidiP97371.
KOiK06696.
OMAiTQISKYY.
PhylomeDBiP97371.
TreeFamiTF106236.

Enzyme and pathway databases

ReactomeiREACT_274287. Degradation of beta-catenin by the destruction complex.
REACT_275686. Activation of NF-kappaB in B cells.
REACT_275732. degradation of AXIN.
REACT_278878. AUF1 (hnRNP D0) destabilizes mRNA.
REACT_289441. Regulation of activated PAK-2p34 by proteasome mediated degradation.
REACT_294625. Ubiquitin-dependent degradation of Cyclin D1.
REACT_297888. degradation of DVL.
REACT_299120. Autodegradation of the E3 ubiquitin ligase COP1.
REACT_301078. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
REACT_310780. Degradation of GLI2 by the proteasome.
REACT_315933. Orc1 removal from chromatin.
REACT_321346. Separation of Sister Chromatids.
REACT_324511. Degradation of GLI1 by the proteasome.
REACT_326233. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
REACT_329431. APC/C:Cdc20 mediated degradation of Securin.
REACT_331452. Hh ligand biogenesis disease.
REACT_334737. Regulation of ornithine decarboxylase (ODC).
REACT_336463. CDK-mediated phosphorylation and removal of Cdc6.
REACT_336745. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
REACT_337766. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_341044. SCF-beta-TrCP mediated degradation of Emi1.
REACT_341075. ER-Phagosome pathway.
REACT_342086. SCF(Skp2)-mediated degradation of p27/p21.
REACT_342636. Asymmetric localization of PCP proteins.
REACT_343561. CDT1 association with the CDC6:ORC:origin complex.
REACT_343568. Antigen processing: Ubiquitination & Proteasome degradation.
REACT_345193. Cross-presentation of soluble exogenous antigens (endosomes).
REACT_347264. Hedgehog ligand biogenesis.
REACT_351311. Hedgehog 'on' state.
REACT_353777. GLI3 is processed to GLI3R by the proteasome.

Miscellaneous databases

NextBioi295890.
PROiP97371.
SOURCEiSearch...

Gene expression databases

BgeeiP97371.
CleanExiMM_PSME1.
ExpressionAtlasiP97371. baseline and differential.
GenevestigatoriP97371.

Family and domain databases

Gene3Di1.20.120.180. 1 hit.
1.20.5.120. 1 hit.
InterProiIPR009077. Proteasome_activ_pa28.
IPR003186. Proteasome_activ_pa28_C.
IPR003185. Proteasome_activ_pa28_N.
[Graphical view]
PANTHERiPTHR10660. PTHR10660. 1 hit.
PfamiPF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
[Graphical view]
SUPFAMiSSF47216. SSF47216. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "PA28 subunits of the mouse proteasome: primary structures and chromosomal localization of the genes."
    Kandil E., Kohda K., Ishibashi T., Tanaka K., Kasahara M.
    Immunogenetics 46:337-344(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
    Tissue: Spleen.
  2. "Sequence and expression of mouse proteasome activator PA28 and the related autoantigen Ki."
    Jiang H., Monaco J.J.
    Immunogenetics 46:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: B10.BR.
  3. "Characterization of the mouse PA28 activator complex gene family: complete organizations of the three member genes and a physical map of the approximately 150-kb region containing the alpha- and beta-subunit genes."
    Kohda K., Ishibashi T., Shimbara N., Tanaka K., Matsuda Y., Kasahara M.
    J. Immunol. 160:4923-4935(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  4. "Nucleotide sequence analysis of the ~35-kb segment containing interferon-gamma-inducible mouse proteasome activator genes."
    Yawata M., Murata S., Tanaka K., Ishigatsubo Y., Kasahara M.
    Immunogenetics 53:119-129(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: 129/SvJ.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryonic head and Small intestine.

Entry informationi

Entry nameiPSME1_MOUSE
AccessioniPrimary (citable) accession number: P97371
Secondary accession number(s): O35561, Q9D841
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: April 1, 2015
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.