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Protein

Proteasome activator complex subunit 1

Gene

Psme1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Implicated in immunoproteasome assembly and required for efficient antigen processing. The PA28 activator complex enhances the generation of class I binding peptides by altering the cleavage pattern of the proteasome.

GO - Molecular functioni

  • endopeptidase activator activity Source: MGI

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-1169091. Activation of NF-kappaB in B cells.
R-MMU-1234176. Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha.
R-MMU-1236978. Cross-presentation of soluble exogenous antigens (endosomes).
R-MMU-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-MMU-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-MMU-174154. APC/C:Cdc20 mediated degradation of Securin.
R-MMU-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-MMU-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-MMU-187577. SCF(Skp2)-mediated degradation of p27/p21.
R-MMU-195253. Degradation of beta-catenin by the destruction complex.
R-MMU-202424. Downstream TCR signaling.
R-MMU-2467813. Separation of Sister Chromatids.
R-MMU-2871837. FCERI mediated NF-kB activation.
R-MMU-349425. Autodegradation of the E3 ubiquitin ligase COP1.
R-MMU-350562. Regulation of ornithine decarboxylase (ODC).
R-MMU-382556. ABC-family proteins mediated transport.
R-MMU-446652. Interleukin-1 family signaling.
R-MMU-450408. AUF1 (hnRNP D0) binds and destabilizes mRNA.
R-MMU-4608870. Asymmetric localization of PCP proteins.
R-MMU-4641257. Degradation of AXIN.
R-MMU-4641258. Degradation of DVL.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-5607761. Dectin-1 mediated noncanonical NF-kB signaling.
R-MMU-5607764. CLEC7A (Dectin-1) signaling.
R-MMU-5610780. Degradation of GLI1 by the proteasome.
R-MMU-5610785. GLI3 is processed to GLI3R by the proteasome.
R-MMU-5632684. Hedgehog 'on' state.
R-MMU-5658442. Regulation of RAS by GAPs.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676590. NIK-->noncanonical NF-kB signaling.
R-MMU-5687128. MAPK6/MAPK4 signaling.
R-MMU-5689603. UCH proteinases.
R-MMU-5689880. Ub-specific processing proteases.
R-MMU-68827. CDT1 association with the CDC6:ORC:origin complex.
R-MMU-68949. Orc1 removal from chromatin.
R-MMU-69017. CDK-mediated phosphorylation and removal of Cdc6.
R-MMU-69229. Ubiquitin-dependent degradation of Cyclin D1.
R-MMU-69481. G2/M Checkpoints.
R-MMU-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-MMU-8852276. The role of GTSE1 in G2/M progression after G2 checkpoint.
R-MMU-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-MMU-8939236. RUNX1 regulates transcription of genes involved in differentiation of HSCs.
R-MMU-8939902. Regulation of RUNX2 expression and activity.
R-MMU-8941858. Regulation of RUNX3 expression and activity.
R-MMU-8948751. Regulation of PTEN stability and activity.
R-MMU-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome activator complex subunit 1
Alternative name(s):
11S regulator complex subunit alpha
Short name:
REG-alpha
Activator of multicatalytic protease subunit 1
Proteasome activator 28 subunit alpha
Short name:
PA28a
Short name:
PA28alpha
Gene namesi
Name:Psme1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1096367. Psme1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001617811 – 249Proteasome activator complex subunit 1Add BLAST249

Proteomic databases

EPDiP97371.
PaxDbiP97371.
PRIDEiP97371.

PTM databases

iPTMnetiP97371.
PhosphoSitePlusiP97371.

Expressioni

Inductioni

By interferon gamma.

Gene expression databases

BgeeiENSMUSG00000022216.
CleanExiMM_PSME1.
ExpressionAtlasiP97371. baseline and differential.
GenevisibleiP97371. MM.

Interactioni

Subunit structurei

Heterodimer of PSME1 and PSME2, which forms a hexameric ring. PSME1 can form homoheptamers (By similarity).By similarity

Protein-protein interaction databases

BioGridi202432. 1 interactor.
IntActiP97371. 3 interactors.
MINTiMINT-1844401.
STRINGi10090.ENSMUSP00000134735.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi8 – 30Combined sources23
Helixi32 – 45Combined sources14
Helixi47 – 49Combined sources3
Helixi54 – 57Combined sources4
Helixi69 – 72Combined sources4
Helixi108 – 137Combined sources30
Beta strandi144 – 146Combined sources3
Helixi148 – 190Combined sources43
Helixi196 – 232Combined sources37
Helixi234 – 238Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5MSJX-ray3.50A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R/S/T/U/V/W/X/Y/Z/a/b1-249[»]
5MX5X-ray2.90A/C/E/G/H/J/L/N1-249[»]
ProteinModelPortaliP97371.
SMRiP97371.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PA28 family.Curated

Phylogenomic databases

eggNOGiKOG4470. Eukaryota.
ENOG410XQVX. LUCA.
GeneTreeiENSGT00510000046374.
HOGENOMiHOG000282822.
HOVERGENiHBG053745.
InParanoidiP97371.
KOiK06696.
OMAiTQISKYY.
OrthoDBiEOG091G0GX7.
PhylomeDBiP97371.
TreeFamiTF106236.

Family and domain databases

Gene3Di1.20.120.180. 1 hit.
1.20.5.120. 1 hit.
InterProiView protein in InterPro
IPR003186. PA28_C.
IPR036997. PA28_C_sf.
IPR036996. PA28_N_sf.
IPR009077. Proteasome_activ_PA28.
IPR003185. Proteasome_activ_PA28_N.
IPR036252. Proteasome_activ_sf.
PANTHERiPTHR10660. PTHR10660. 1 hit.
PfamiView protein in Pfam
PF02251. PA28_alpha. 1 hit.
PF02252. PA28_beta. 1 hit.
SUPFAMiSSF47216. SSF47216. 2 hits.

Sequencei

Sequence statusi: Complete.

P97371-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLRVHPEA QAKVDVFRED LCSKTENLLG SYFPKKISEL DAFLKEPALN
60 70 80 90 100
EANLSNLKAP LDIPVPDPVK EKEKEERKKQ QEKEEKEEKK KGDEDDKGPP
110 120 130 140 150
CGPVNCNEKI VVLLQRLKPE IKDVTEQLNL VTTWLQLQIP RIEDGNNFGV
160 170 180 190 200
AVQEKVFELM TNLHTKLEGF HTQISKYFSE RGDAVAKAAK QPHVGDYRQL
210 220 230 240
VHELDEAEYQ EIRLMVMEIR NAYAVLYDII LKNFEKLKKP RGETKGMIY
Length:249
Mass (Da):28,673
Last modified:August 29, 2001 - v2
Checksum:i1E2CE09DA44A5346
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17F → L in BAB25712 (PubMed:16141072).Curated1
Sequence conflicti97K → E in BAB25712 (PubMed:16141072).Curated1
Sequence conflicti151A → S in AAC53295 (PubMed:9162094).Curated1
Sequence conflicti156V → L in AAC53295 (PubMed:9162094).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87909 mRNA. Translation: BAA22039.1.
U60328 mRNA. Translation: AAC53295.1.
AB007136 Genomic DNA. Translation: BAA28835.1.
AB053120 Genomic DNA. Translation: BAB47403.1.
AK019390 mRNA. Translation: BAB31696.1.
AK008514 mRNA. Translation: BAB25712.1.
CCDSiCCDS36931.1.
RefSeqiNP_035319.1. NM_011189.1.
UniGeneiMm.830.

Genome annotation databases

EnsembliENSMUST00000174259; ENSMUSP00000134735; ENSMUSG00000022216.
GeneIDi19186.
KEGGimmu:19186.
UCSCiuc007tzd.1. mouse.

Similar proteinsi

Entry informationi

Entry nameiPSME1_MOUSE
AccessioniPrimary (citable) accession number: P97371
Secondary accession number(s): O35561, Q9D841
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: August 29, 2001
Last modified: November 22, 2017
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families