P97370 (AT1B3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sodium/potassium-transporting ATPase subunit beta-3

Alternative name(s):
Sodium/potassium-dependent ATPase subunit beta-3
Short name=ATPB-3
CD_antigen=CD298

Gene names

Name:

Atp1b3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known.
Subunit structure	Composed of three subunits: alpha (catalytic), beta and gamma.
Subcellular location	Membrane; Single-pass type II membrane protein. Melanosome By similarity.
Tissue specificity	Widely expressed.
Sequence similarities	Belongs to the X(+)/potassium ATPases subunit beta family.

Ontologies

Keywords
Biological process	Ion transport Potassium transport Sodium transport Sodium/potassium transport Transport
Cellular component	Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Ligand	Potassium Sodium
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Sodium/potassium-transporting ATPase subunit beta-3		PRO_0000219109
Regions
	Topological domain	1 – 35	35	Cytoplasmic Potential
	Transmembrane	36 – 56	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	57 – 278	222	Extracellular Potential
Amino acid modifications
	Glycosylation	124	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	197	1	N-linked (GlcNAc...) Ref.3 Ref.4
	Disulfide bond	128 ↔ 144		By similarity
	Disulfide bond	154 ↔ 170		By similarity
	Disulfide bond	191 ↔ 249		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97370 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 98A46FF6EDA85AAC	FASTA	278	31,776
	10 20 30 40 50 60 MTKTEKKSFH QSLAEWKLFI YNPSSGEFLG RTSKSWGLIL LFYLVFYGFL AALFTFTMWA 70 80 90 100 110 120 MLQTLNDEVP KYRDQIPSPG LMVFPKPQTA LEYTFSMSEP QTYKKLVEDL ESFLKPYSVE 130 140 150 160 170 180 EQKNLTSCPD GAPFIQHGPD YRACQFPVSL LEECSGVTDA NFGYSKGQPC ILVKMNRIID 190 200 210 220 230 240 LIPDGYPQIS CLPKEENATI ATYPEFGVLD LKYFPYYGKK RHVGYRQPLV AVQVKFDSGL 250 260 270 NKKEVTVECH IAGTRNLKNK NERDKFLGRV SFKVTARA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel beta 3 isoform of the Na,K-ATPase beta subunit from mouse retina." Besirli C.G., Gong T.-W.L., Lomax M.I. Biochim. Biophys. Acta 1350:21-26(1997) [PubMed: 9003452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Retina.
[2]	Lubec G., Kang S.U. Submitted (APR-2007) to UniProtKB Cited for: PROTEIN SEQUENCE OF 18-31, MASS SPECTROMETRY. Strain: C57BL/6. Tissue: Brain.
[3]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed: 19656770] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-197, MASS SPECTROMETRY. Tissue: Myoblast.
[4]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-124 AND ASN-197, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U59761 mRNA. Translation: AAC00019.1.
IPI	IPI00124221.
RefSeq	NP_031528.1. NM_007502.4.
UniGene	Mm.424.
3D structure databases
ProteinModelPortal	P97370.
SMR	P97370. Positions 22-278.
ModBase	Search...
Protein-protein interaction databases
STRING	P97370.
PTM databases
PhosphoSite	P97370.
Proteomic databases
PRIDE	P97370.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000034983; ENSMUSP00000034983; ENSMUSG00000032412.
GeneID	11933.
KEGG	mmu:11933.
Organism-specific databases
CTD	483.
MGI	MGI:107788. Atp1b3.
Phylogenomic databases
eggNOG	roNOG06299.
HOGENOM	HBG446583.
HOVERGEN	HBG050603.
InParanoid	P97370.
OMA	LMISPKP.
OrthoDB	EOG447FTQ.
PhylomeDB	P97370.
Gene expression databases
ArrayExpress	P97370.
Bgee	P97370.
CleanEx	MM_ATP1B3.
Genevestigator	P97370.
GermOnline	ENSMUSG00000032412. Mus musculus.
Family and domain databases
InterPro	IPR000402. ATPase_P-typ_cation-exchng_bsu. [Graphical view]
KO	K01540.
PANTHER	PTHR11523. ATPase_H_Na/K_b. 1 hit.
Pfam	PF00287. Na_K-ATPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01107. Na_K_ATPase_bet. 1 hit.
PROSITE	PS00390. ATPASE_NA_K_BETA_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	280025.
SOURCE	Search...

Entry information

Entry name

AT1B3_MOUSE

Accession

Primary (citable) accession number: P97370

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program