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P97367 (MEIS2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Meis2
Alternative name(s):
Meis1-related protein 1
Gene names
Name:Meis2
Synonyms:Mrg1, Stra10
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length477 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in transcriptional regulation. Binds to HOX or PBX proteins to form dimers, or to a DNA-bound dimer of PBX and HOX proteins and thought to have a role in stabilization of the homeoprotein-DNA complex. Isoform 3/Meis2Bis required for the activity of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element; MEIS2 is not involved in complex DNA-binding. Probably in complex with PBX1, is involved in transcriptional regulation by KLF4. Isoforms 3/Meis2B and 4/Meis2D can bind to a EPHA8 promoter sequence containing the DNA motif 5'-CGGTCA-3'; in cooperation with a PBX protein (such as PBX2) is proposed to be involved in the transcriptional activation of EPHA8 in the developing midbrain. May be involved in regulation of myeloid differentiation. Can bind to the DNA sequence 5'-TGACAG-3'in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription. Ref.6 Ref.7 Ref.9

Subunit structure

Isoform Meis2A interacts with TLX1. Isoform Meis2B interacts with HOXA13 and PBX1 isoform PBX1b Isoform Meis2D interacts with SP1, SP3 and KLF4. Isoform Meis2D interacts with PBX1 isoform PBX1a;the interaction partially relieves MEIS2 autoinhibition. Isoform Meis2B is part of a PDX1:PBX1b:MEIS2b complex; Meis2B is recruited by PBX1b and can be replaced by isoform Meis2D in a small fraction of complexes. Can form trimeric complexes including HOXB8 and PBX2 or PBX3. Ref.4 Ref.6 Ref.7 Ref.8

Subcellular location

Nucleus Ref.4.

Tissue specificity

Displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Lower expression in lung and only basal level in heart, liver, kidney, spleen, and testis.

Developmental stage

Expressed at high levels in all stages of embryonic development analyzed (7 days to 17 days). First detected around E10.5 in the developing ventrolateral telencephalon. Is found at moderate levels throughout the ventricle zone (VZ) of the entire telencephalon with the exception of the ventro- and dorso-medial regions. The highest expression is detected in the subventricular zone (SVZ) of the lateral ganglionic eminence (LGE) and developing striatum. By E16.5, also found in the cortical plate. Also expressed at high levels in the caudal ganglionic eminence (CGE) and amygdala. Expression in the telencephalon remains unchanged at birth and into adulthood. Ref.5

Induction

By retinoic acid.

Sequence similarities

Belongs to the TALE/MEIS homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
   DomainHomeobox
   LigandDNA-binding
   Molecular functionActivator
Developmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processeye development

Inferred from genetic interaction PubMed 12183364. Source: MGI

negative regulation of myeloid cell differentiation

Inferred from direct assay Ref.6. Source: UniProtKB

pancreas development

Inferred from electronic annotation. Source: Ensembl

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction Ref.9. Source: MGI

response to growth factor

Inferred from electronic annotation. Source: Ensembl

response to mechanical stimulus

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentnucleus

Inferred from direct assay Ref.5PubMed 11358480Ref.8PubMed 17049510Ref.9. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12183364PubMed 15465489. Source: MGI

sequence-specific DNA binding

Inferred from direct assay Ref.9PubMed 18787068. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay Ref.9. Source: MGI

transcription cofactor activity

Inferred from direct assay Ref.7. Source: UniProtKB

transcription factor binding

Inferred from direct assay Ref.7Ref.6. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Note: Additional isoforms seem to exist.
Isoform Meis2C (identifier: P97367-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Meis2A (identifier: P97367-2)

The sequence of this isoform differs from the canonical sequence as follows:
     384-401: LQSMPGDYVSQGGPMGMG → PMSGMGMNMGMDGQWHYM
     402-477: Missing.
Isoform Meis2B (identifier: P97367-3)

Also known as: Mrg1A;

The sequence of this isoform differs from the canonical sequence as follows:
     346-352: Missing.
     384-401: LQSMPGDYVSQGGPMGMG → PMSGMGMNMGMDGQWHYM
     402-477: Missing.
Isoform Meis2D (identifier: P97367-4)

Also known as: Mrg1B;

The sequence of this isoform differs from the canonical sequence as follows:
     346-352: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 477477Homeobox protein Meis2
PRO_0000049109

Regions

DNA binding276 – 33863Homeobox; TALE-type Ref.9
Region71 – 191121Required for interaction with PBX1
Region340 – 477138Transcriptional activation domain By similarity
Compositional bias195 – 24450Ser/Thr-rich
Compositional bias246 – 27328Asp/Glu-rich (acidic)
Compositional bias266 – 2738Poly-Asp

Natural variations

Alternative sequence346 – 3527Missing in isoform Meis2B and isoform Meis2D.
VSP_002247
Alternative sequence384 – 40118LQSMP…PMGMG → PMSGMGMNMGMDGQWHYM in isoform Meis2A and isoform Meis2B.
VSP_002248
Alternative sequence402 – 47776Missing in isoform Meis2A and isoform Meis2B.
VSP_002249

Experimental info

Sequence conflict21A → E in AAC52948. Ref.1
Sequence conflict3161T → A in AAC52948. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform Meis2C [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 923610800B647A21

FASTA47751,728
        10         20         30         40         50         60 
MAQRYDELPH YGGMDGVGVP ASMYGDPHAP RPIPPVHHLN HGPPLHATQH YGAHAPHPNV 

        70         80         90        100        110        120 
MPASMGSAVN DALKRDKDAI YGHPLFPLLA LVFEKCELAT CTPREPGVAG GDVCSSDSFN 

       130        140        150        160        170        180 
EDIAVFAKQV RAEKPLFSSN PELDNLMIQA IQVLRFHLLE LEKVHELCDN FCHRYISCLK 

       190        200        210        220        230        240 
GKMPIDLVID ERDGSSKSDH EELSGSSTNL ADHNPSSWRD HDDATSTHSA GTPGPSSGGH 

       250        260        270        280        290        300 
ASQSGDNSSE QGDGLDNSVA SPGTGDDDDP DKDKKRQKKR GIFPKVATNI MRAWLFQHLT 

       310        320        330        340        350        360 
HPYPSEEQKK QLAQDTGLTI LQVNNWFINA RRRIVQPMID QSNRAGFLLD PSVSQGAAYS 

       370        380        390        400        410        420 
PEGQPMGSFV LDGQQHMGIR PAGLQSMPGD YVSQGGPMGM GMAQPSYTPP QMTPHPTQLR 

       430        440        450        460        470 
HGPPMHSYLP SHPHHPAMVM HGGPPTHPGM TMSAQSPTML NSVDPNVGGQ VMDIHAQ 

« Hide

Isoform Meis2A [UniParc].

Checksum: 0682C0E92326516D
Show »

FASTA40143,791
Isoform Meis2B (Mrg1A) [UniParc].

Checksum: 6BCC0382F0C55296
Show »

FASTA39443,061
Isoform Meis2D (Mrg1B) [UniParc].

Checksum: C78142B9E15CDDBC
Show »

FASTA47050,998

References

[1]"Identification of a new family of Pbx-related homeobox genes."
Nakamura T., Jenkins N.A., Copeland N.G.
Oncogene 13:2235-2242(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MEIS2A).
Strain: Swiss Webster.
[2]"Meis2, a novel mouse Pbx-related homeobox gene induced by retinoic acid during differentiation of P19 embryonal carcinoma cells."
Oulad-Abdelghani M., Chazaud C., Bouillet P., Sapin V., Chambon P., Dolle P.
Dev. Dyn. 210:173-183(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MEIS2A; MEIS2B; MEIS2C AND MEIS2D).
[3]"Identification of a conserved family of Meis1-related homeobox genes."
Steelman S., Moskow J.J., Muzynski K., North C., Druck T., Montgomery J.C., Huebner K., Daar I.O., Buchberg A.M.
Genome Res. 7:142-156(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MEIS2B AND MEIS2D).
[4]"An endocrine-exocrine switch in the activity of the pancreatic homeodomain protein PDX1 through formation of a trimeric complex with PBX1b and MRG1 (MEIS2)."
Swift G.H., Liu Y., Rose S.D., Bischof L.J., Steelman S., Buchberg A.M., Wright C.V., MacDonald R.J.
Mol. Cell. Biol. 18:5109-5120(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH PDX1 AND PBX1.
[5]"Expression of Meis and Pbx genes and their protein products in the developing telencephalon: implications for regional differentiation."
Toresson H., Parmar M., Campbell K.
Mech. Dev. 94:183-187(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[6]"Inhibition of myeloid differentiation by Hoxa9, Hoxb8, and Meis homeobox genes."
Fujino T., Yamazaki Y., Largaespada D.A., Jenkins N.A., Copeland N.G., Hirokawa K., Nakamura T.
Exp. Hematol. 29:856-863(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[7]"DNA binding and transcriptional activation by a PDX1.PBX1b.MEIS2b trimer and cooperation with a pancreas-specific basic helix-loop-helix complex."
Liu Y., MacDonald R.J., Swift G.H.
J. Biol. Chem. 276:17985-17993(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH PBX1, IDENTIFICATION IN A COMPLEX WITH PDX1 AND PBX1.
[8]"Range of HOX/TALE superclass associations and protein domain requirements for HOXA13:MEIS interaction."
Williams T.M., Williams M.E., Innis J.W.
Dev. Biol. 277:457-471(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HOXA13.
[9]"Regulation of EphA8 gene expression by TALE homeobox transcription factors during development of the mesencephalon."
Shim S., Kim Y., Shin J., Kim J., Park S.
Mol. Cell. Biol. 27:1614-1630(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DNA-BINDING.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U57343 mRNA. Translation: AAC52948.1.
AJ000504 mRNA. Translation: CAA04138.1.
AJ000505 mRNA. Translation: CAA04139.1.
AJ000506 mRNA. Translation: CAA04140.1.
AJ000507 mRNA. Translation: CAA04141.1.
U68383 mRNA. Translation: AAB19193.1.
U68384 mRNA. Translation: AAB19194.1.
RefSeqNP_001129544.1. NM_001136072.2.
NP_001153039.1. NM_001159567.1.
NP_001153040.1. NM_001159568.1.
NP_001153041.1. NM_001159569.1.
NP_034955.1. NM_010825.3.
UniGeneMm.247566.
Mm.471935.

3D structure databases

ProteinModelPortalP97367.
SMRP97367. Positions 283-338.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201487. 8 interactions.
IntActP97367. 1 interaction.

PTM databases

PhosphoSiteP97367.

Proteomic databases

PaxDbP97367.
PRIDEP97367.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000028639; ENSMUSP00000028639; ENSMUSG00000027210. [P97367-1]
ENSMUST00000102538; ENSMUSP00000099597; ENSMUSG00000027210. [P97367-4]
ENSMUST00000110907; ENSMUSP00000106532; ENSMUSG00000027210. [P97367-2]
ENSMUST00000110908; ENSMUSP00000106533; ENSMUSG00000027210. [P97367-3]
GeneID17536.
KEGGmmu:17536.
UCSCuc008lqy.2. mouse. [P97367-1]

Organism-specific databases

CTD4212.
MGIMGI:108564. Meis2.

Phylogenomic databases

eggNOGNOG302919.
GeneTreeENSGT00550000074260.
HOGENOMHOG000253923.
HOVERGENHBG055193.
InParanoidP97367.
KOK16670.
OMASQHYGAH.
OrthoDBEOG76HQ2W.
PhylomeDBP97367.
TreeFamTF318093.

Gene expression databases

ArrayExpressP97367.
BgeeP97367.
CleanExMM_MEIS2.
GenevestigatorP97367.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio292164.
PROP97367.
SOURCESearch...

Entry information

Entry nameMEIS2_MOUSE
AccessionPrimary (citable) accession number: P97367
Secondary accession number(s): O35676 expand/collapse secondary AC list , O35677, P97403, P97404
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot