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P97367

- MEIS2_MOUSE

UniProt

P97367 - MEIS2_MOUSE

Protein

Homeobox protein Meis2

Gene

Meis2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    Involved in transcriptional regulation. Binds to HOX or PBX proteins to form dimers, or to a DNA-bound dimer of PBX and HOX proteins and thought to have a role in stabilization of the homeoprotein-DNA complex. Isoform 3/Meis2B is required for the activity of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element; MEIS2 is not involved in complex DNA-binding. Probably in complex with PBX1, is involved in transcriptional regulation by KLF4. Isoforms 3/Meis2B and 4/Meis2D can bind to a EPHA8 promoter sequence containing the DNA motif 5'-CGGTCA-3'; in cooperation with a PBX protein (such as PBX2) is proposed to be involved in the transcriptional activation of EPHA8 in the developing midbrain. May be involved in regulation of myeloid differentiation. Can bind to the DNA sequence 5'-TGACAG-3'in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription.3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi276 – 33863Homeobox; TALE-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. sequence-specific DNA binding Source: MGI
    3. sequence-specific DNA binding transcription factor activity Source: InterPro
    4. transcription cofactor activity Source: UniProtKB
    5. transcription factor binding Source: UniProtKB

    GO - Biological processi

    1. eye development Source: MGI
    2. negative regulation of myeloid cell differentiation Source: UniProtKB
    3. pancreas development Source: Ensembl
    4. response to growth factor Source: Ensembl
    5. response to mechanical stimulus Source: Ensembl
    6. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Homeobox protein Meis2
    Alternative name(s):
    Meis1-related protein 1
    Gene namesi
    Name:Meis2
    Synonyms:Mrg1, Stra10
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:108564. Meis2.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 477477Homeobox protein Meis2PRO_0000049109Add
    BLAST

    Proteomic databases

    MaxQBiP97367.
    PaxDbiP97367.
    PRIDEiP97367.

    PTM databases

    PhosphoSiteiP97367.

    Expressioni

    Tissue specificityi

    Displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Lower expression in lung and only basal level in heart, liver, kidney, spleen, and testis.

    Developmental stagei

    Expressed at high levels in all stages of embryonic development analyzed (7 days to 17 days). First detected around E10.5 in the developing ventrolateral telencephalon. Is found at moderate levels throughout the ventricle zone (VZ) of the entire telencephalon with the exception of the ventro- and dorso-medial regions. The highest expression is detected in the subventricular zone (SVZ) of the lateral ganglionic eminence (LGE) and developing striatum. By E16.5, also found in the cortical plate. Also expressed at high levels in the caudal ganglionic eminence (CGE) and amygdala. Expression in the telencephalon remains unchanged at birth and into adulthood.1 Publication

    Inductioni

    By retinoic acid.

    Gene expression databases

    ArrayExpressiP97367.
    BgeeiP97367.
    CleanExiMM_MEIS2.
    GenevestigatoriP97367.

    Interactioni

    Subunit structurei

    Isoform Meis2A interacts with TLX1. Isoform Meis2B interacts with HOXA13 and PBX1 isoform PBX1b. Isoform Meis2D interacts with SP1, SP3 and KLF4. Isoform Meis2D interacts with PBX1 isoform PBX1a; the interaction partially relieves MEIS2 autoinhibition. Isoform Meis2B is part of a PDX1:PBX1b:MEIS2b complex; Meis2B is recruited by PBX1b and can be replaced by isoform Meis2D in a small fraction of complexes. Can form trimeric complexes including HOXB8 and PBX2 or PBX3.4 Publications

    Protein-protein interaction databases

    BioGridi201487. 8 interactions.
    IntActiP97367. 1 interaction.

    Structurei

    3D structure databases

    ProteinModelPortaliP97367.
    SMRiP97367. Positions 283-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni71 – 191121Required for interaction with PBX1Add
    BLAST
    Regioni340 – 477138Transcriptional activation domainBy similarityAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi195 – 24450Ser/Thr-richAdd
    BLAST
    Compositional biasi246 – 27328Asp/Glu-rich (acidic)Add
    BLAST
    Compositional biasi266 – 2738Poly-Asp

    Sequence similaritiesi

    Belongs to the TALE/MEIS homeobox family.Curated
    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG302919.
    GeneTreeiENSGT00550000074260.
    HOGENOMiHOG000253923.
    HOVERGENiHBG055193.
    InParanoidiP97367.
    KOiK16670.
    OMAiSQHYGAH.
    OrthoDBiEOG76HQ2W.
    PhylomeDBiP97367.
    TreeFamiTF318093.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    InterProiIPR001356. Homeobox_dom.
    IPR008422. Homeobox_KN_domain.
    IPR009057. Homeodomain-like.
    [Graphical view]
    PfamiPF05920. Homeobox_KN. 1 hit.
    [Graphical view]
    SMARTiSM00389. HOX. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    PROSITEiPS50071. HOMEOBOX_2. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Note: Additional isoforms seem to exist.

    Isoform Meis2C (identifier: P97367-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAQRYDELPH YGGMDGVGVP ASMYGDPHAP RPIPPVHHLN HGPPLHATQH    50
    YGAHAPHPNV MPASMGSAVN DALKRDKDAI YGHPLFPLLA LVFEKCELAT 100
    CTPREPGVAG GDVCSSDSFN EDIAVFAKQV RAEKPLFSSN PELDNLMIQA 150
    IQVLRFHLLE LEKVHELCDN FCHRYISCLK GKMPIDLVID ERDGSSKSDH 200
    EELSGSSTNL ADHNPSSWRD HDDATSTHSA GTPGPSSGGH ASQSGDNSSE 250
    QGDGLDNSVA SPGTGDDDDP DKDKKRQKKR GIFPKVATNI MRAWLFQHLT 300
    HPYPSEEQKK QLAQDTGLTI LQVNNWFINA RRRIVQPMID QSNRAGFLLD 350
    PSVSQGAAYS PEGQPMGSFV LDGQQHMGIR PAGLQSMPGD YVSQGGPMGM 400
    GMAQPSYTPP QMTPHPTQLR HGPPMHSYLP SHPHHPAMVM HGGPPTHPGM 450
    TMSAQSPTML NSVDPNVGGQ VMDIHAQ 477
    Length:477
    Mass (Da):51,728
    Last modified:December 15, 1998 - v2
    Checksum:i923610800B647A21
    GO
    Isoform Meis2A (identifier: P97367-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         384-401: LQSMPGDYVSQGGPMGMG → PMSGMGMNMGMDGQWHYM
         402-477: Missing.

    Show »
    Length:401
    Mass (Da):43,791
    Checksum:i0682C0E92326516D
    GO
    Isoform Meis2B (identifier: P97367-3) [UniParc]FASTAAdd to Basket

    Also known as: Mrg1A

    The sequence of this isoform differs from the canonical sequence as follows:
         346-352: Missing.
         384-401: LQSMPGDYVSQGGPMGMG → PMSGMGMNMGMDGQWHYM
         402-477: Missing.

    Show »
    Length:394
    Mass (Da):43,061
    Checksum:i6BCC0382F0C55296
    GO
    Isoform Meis2D (identifier: P97367-4) [UniParc]FASTAAdd to Basket

    Also known as: Mrg1B

    The sequence of this isoform differs from the canonical sequence as follows:
         346-352: Missing.

    Show »
    Length:470
    Mass (Da):50,998
    Checksum:iC78142B9E15CDDBC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti2 – 21A → E in AAC52948. (PubMed:8950991)Curated
    Sequence conflicti316 – 3161T → A in AAC52948. (PubMed:8950991)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei346 – 3527Missing in isoform Meis2B and isoform Meis2D. 2 PublicationsVSP_002247
    Alternative sequencei384 – 40118LQSMP…PMGMG → PMSGMGMNMGMDGQWHYM in isoform Meis2A and isoform Meis2B. 3 PublicationsVSP_002248Add
    BLAST
    Alternative sequencei402 – 47776Missing in isoform Meis2A and isoform Meis2B. 3 PublicationsVSP_002249Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57343 mRNA. Translation: AAC52948.1.
    AJ000504 mRNA. Translation: CAA04138.1.
    AJ000505 mRNA. Translation: CAA04139.1.
    AJ000506 mRNA. Translation: CAA04140.1.
    AJ000507 mRNA. Translation: CAA04141.1.
    U68383 mRNA. Translation: AAB19193.1.
    U68384 mRNA. Translation: AAB19194.1.
    CCDSiCCDS16568.1. [P97367-4]
    CCDS50666.1. [P97367-3]
    CCDS50667.1. [P97367-1]
    CCDS50668.1. [P97367-2]
    RefSeqiNP_001129544.1. NM_001136072.2. [P97367-3]
    NP_001153039.1. NM_001159567.1. [P97367-2]
    NP_001153040.1. NM_001159568.1. [P97367-1]
    NP_001153041.1. NM_001159569.1.
    NP_034955.1. NM_010825.3. [P97367-4]
    UniGeneiMm.247566.
    Mm.471935.

    Genome annotation databases

    EnsembliENSMUST00000028639; ENSMUSP00000028639; ENSMUSG00000027210. [P97367-1]
    ENSMUST00000102538; ENSMUSP00000099597; ENSMUSG00000027210. [P97367-4]
    ENSMUST00000110907; ENSMUSP00000106532; ENSMUSG00000027210. [P97367-2]
    ENSMUST00000110908; ENSMUSP00000106533; ENSMUSG00000027210. [P97367-3]
    GeneIDi17536.
    KEGGimmu:17536.
    UCSCiuc008lqy.2. mouse. [P97367-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U57343 mRNA. Translation: AAC52948.1 .
    AJ000504 mRNA. Translation: CAA04138.1 .
    AJ000505 mRNA. Translation: CAA04139.1 .
    AJ000506 mRNA. Translation: CAA04140.1 .
    AJ000507 mRNA. Translation: CAA04141.1 .
    U68383 mRNA. Translation: AAB19193.1 .
    U68384 mRNA. Translation: AAB19194.1 .
    CCDSi CCDS16568.1. [P97367-4 ]
    CCDS50666.1. [P97367-3 ]
    CCDS50667.1. [P97367-1 ]
    CCDS50668.1. [P97367-2 ]
    RefSeqi NP_001129544.1. NM_001136072.2. [P97367-3 ]
    NP_001153039.1. NM_001159567.1. [P97367-2 ]
    NP_001153040.1. NM_001159568.1. [P97367-1 ]
    NP_001153041.1. NM_001159569.1.
    NP_034955.1. NM_010825.3. [P97367-4 ]
    UniGenei Mm.247566.
    Mm.471935.

    3D structure databases

    ProteinModelPortali P97367.
    SMRi P97367. Positions 283-338.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201487. 8 interactions.
    IntActi P97367. 1 interaction.

    PTM databases

    PhosphoSitei P97367.

    Proteomic databases

    MaxQBi P97367.
    PaxDbi P97367.
    PRIDEi P97367.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000028639 ; ENSMUSP00000028639 ; ENSMUSG00000027210 . [P97367-1 ]
    ENSMUST00000102538 ; ENSMUSP00000099597 ; ENSMUSG00000027210 . [P97367-4 ]
    ENSMUST00000110907 ; ENSMUSP00000106532 ; ENSMUSG00000027210 . [P97367-2 ]
    ENSMUST00000110908 ; ENSMUSP00000106533 ; ENSMUSG00000027210 . [P97367-3 ]
    GeneIDi 17536.
    KEGGi mmu:17536.
    UCSCi uc008lqy.2. mouse. [P97367-1 ]

    Organism-specific databases

    CTDi 4212.
    MGIi MGI:108564. Meis2.

    Phylogenomic databases

    eggNOGi NOG302919.
    GeneTreei ENSGT00550000074260.
    HOGENOMi HOG000253923.
    HOVERGENi HBG055193.
    InParanoidi P97367.
    KOi K16670.
    OMAi SQHYGAH.
    OrthoDBi EOG76HQ2W.
    PhylomeDBi P97367.
    TreeFami TF318093.

    Miscellaneous databases

    NextBioi 292164.
    PROi P97367.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97367.
    Bgeei P97367.
    CleanExi MM_MEIS2.
    Genevestigatori P97367.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    InterProi IPR001356. Homeobox_dom.
    IPR008422. Homeobox_KN_domain.
    IPR009057. Homeodomain-like.
    [Graphical view ]
    Pfami PF05920. Homeobox_KN. 1 hit.
    [Graphical view ]
    SMARTi SM00389. HOX. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    PROSITEi PS50071. HOMEOBOX_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a new family of Pbx-related homeobox genes."
      Nakamura T., Jenkins N.A., Copeland N.G.
      Oncogene 13:2235-2242(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MEIS2A).
      Strain: Swiss Webster.
    2. "Meis2, a novel mouse Pbx-related homeobox gene induced by retinoic acid during differentiation of P19 embryonal carcinoma cells."
      Oulad-Abdelghani M., Chazaud C., Bouillet P., Sapin V., Chambon P., Dolle P.
      Dev. Dyn. 210:173-183(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MEIS2A; MEIS2B; MEIS2C AND MEIS2D).
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MEIS2B AND MEIS2D).
    4. "An endocrine-exocrine switch in the activity of the pancreatic homeodomain protein PDX1 through formation of a trimeric complex with PBX1b and MRG1 (MEIS2)."
      Swift G.H., Liu Y., Rose S.D., Bischof L.J., Steelman S., Buchberg A.M., Wright C.V., MacDonald R.J.
      Mol. Cell. Biol. 18:5109-5120(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH PDX1 AND PBX1.
    5. "Expression of Meis and Pbx genes and their protein products in the developing telencephalon: implications for regional differentiation."
      Toresson H., Parmar M., Campbell K.
      Mech. Dev. 94:183-187(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    6. "Inhibition of myeloid differentiation by Hoxa9, Hoxb8, and Meis homeobox genes."
      Fujino T., Yamazaki Y., Largaespada D.A., Jenkins N.A., Copeland N.G., Hirokawa K., Nakamura T.
      Exp. Hematol. 29:856-863(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    7. "DNA binding and transcriptional activation by a PDX1.PBX1b.MEIS2b trimer and cooperation with a pancreas-specific basic helix-loop-helix complex."
      Liu Y., MacDonald R.J., Swift G.H.
      J. Biol. Chem. 276:17985-17993(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH PBX1, IDENTIFICATION IN A COMPLEX WITH PDX1 AND PBX1.
    8. "Range of HOX/TALE superclass associations and protein domain requirements for HOXA13:MEIS interaction."
      Williams T.M., Williams M.E., Innis J.W.
      Dev. Biol. 277:457-471(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HOXA13.
    9. "Regulation of EphA8 gene expression by TALE homeobox transcription factors during development of the mesencephalon."
      Shim S., Kim Y., Shin J., Kim J., Park S.
      Mol. Cell. Biol. 27:1614-1630(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DNA-BINDING.

    Entry informationi

    Entry nameiMEIS2_MOUSE
    AccessioniPrimary (citable) accession number: P97367
    Secondary accession number(s): O35676
    , O35677, P97403, P97404
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3