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P97367

- MEIS2_MOUSE

UniProt

P97367 - MEIS2_MOUSE

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Protein

Homeobox protein Meis2

Gene

Meis2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in transcriptional regulation. Binds to HOX or PBX proteins to form dimers, or to a DNA-bound dimer of PBX and HOX proteins and thought to have a role in stabilization of the homeoprotein-DNA complex. Isoform 3/Meis2B is required for the activity of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element; MEIS2 is not involved in complex DNA-binding. Probably in complex with PBX1, is involved in transcriptional regulation by KLF4. Isoforms 3/Meis2B and 4/Meis2D can bind to a EPHA8 promoter sequence containing the DNA motif 5'-CGGTCA-3'; in cooperation with a PBX protein (such as PBX2) is proposed to be involved in the transcriptional activation of EPHA8 in the developing midbrain. May be involved in regulation of myeloid differentiation. Can bind to the DNA sequence 5'-TGACAG-3'in the activator ACT sequence of the D(1A) dopamine receptor (DRD1) promoter and activate DRD1 transcription.3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi276 – 33863Homeobox; TALE-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: Ensembl
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: Ensembl
  4. sequence-specific DNA binding Source: MGI
  5. transcription cofactor activity Source: UniProtKB
  6. transcription factor binding Source: UniProtKB

GO - Biological processi

  1. eye development Source: MGI
  2. negative regulation of myeloid cell differentiation Source: UniProtKB
  3. pancreas development Source: Ensembl
  4. response to growth factor Source: Ensembl
  5. response to mechanical stimulus Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Meis2
Alternative name(s):
Meis1-related protein 1
Gene namesi
Name:Meis2
Synonyms:Mrg1, Stra10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:108564. Meis2.

Subcellular locationi

Nucleus 1 PublicationPROSITE-ProRule annotation

GO - Cellular componenti

  1. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 477477Homeobox protein Meis2PRO_0000049109Add
BLAST

Proteomic databases

MaxQBiP97367.
PaxDbiP97367.
PRIDEiP97367.

PTM databases

PhosphoSiteiP97367.

Expressioni

Tissue specificityi

Displays spatially restricted expression patterns in the developing nervous system, limbs, face, and in various viscera. In adult, it is mainly expressed in the brain and female genital tract, with a different distribution of the alternative splice forms in these organs. Lower expression in lung and only basal level in heart, liver, kidney, spleen, and testis.

Developmental stagei

Expressed at high levels in all stages of embryonic development analyzed (7 days to 17 days). First detected around E10.5 in the developing ventrolateral telencephalon. Is found at moderate levels throughout the ventricle zone (VZ) of the entire telencephalon with the exception of the ventro- and dorso-medial regions. The highest expression is detected in the subventricular zone (SVZ) of the lateral ganglionic eminence (LGE) and developing striatum. By E16.5, also found in the cortical plate. Also expressed at high levels in the caudal ganglionic eminence (CGE) and amygdala. Expression in the telencephalon remains unchanged at birth and into adulthood.1 Publication

Inductioni

By retinoic acid.

Gene expression databases

BgeeiP97367.
CleanExiMM_MEIS2.
ExpressionAtlasiP97367. baseline and differential.
GenevestigatoriP97367.

Interactioni

Subunit structurei

Isoform Meis2A interacts with TLX1. Isoform Meis2B interacts with HOXA13 and PBX1 isoform PBX1b. Isoform Meis2D interacts with SP1, SP3 and KLF4. Isoform Meis2D interacts with PBX1 isoform PBX1a; the interaction partially relieves MEIS2 autoinhibition. Isoform Meis2B is part of a PDX1:PBX1b:MEIS2b complex; Meis2B is recruited by PBX1b and can be replaced by isoform Meis2D in a small fraction of complexes. Can form trimeric complexes including HOXB8 and PBX2 or PBX3.4 Publications

Protein-protein interaction databases

BioGridi201487. 8 interactions.
IntActiP97367. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP97367.
SMRiP97367. Positions 283-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni71 – 191121Required for interaction with PBX1Add
BLAST
Regioni340 – 477138Transcriptional activation domainBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi195 – 24450Ser/Thr-richAdd
BLAST
Compositional biasi246 – 27328Asp/Glu-rich (acidic)Add
BLAST
Compositional biasi266 – 2738Poly-Asp

Sequence similaritiesi

Belongs to the TALE/MEIS homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG302919.
GeneTreeiENSGT00550000074260.
HOGENOMiHOG000253923.
HOVERGENiHBG055193.
InParanoidiP97367.
KOiK16670.
OMAiSQHYGAH.
OrthoDBiEOG76HQ2W.
PhylomeDBiP97367.
TreeFamiTF318093.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF05920. Homeobox_KN. 1 hit.
[Graphical view]
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Note: Additional isoforms seem to exist.

Isoform Meis2C (identifier: P97367-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAQRYDELPH YGGMDGVGVP ASMYGDPHAP RPIPPVHHLN HGPPLHATQH
60 70 80 90 100
YGAHAPHPNV MPASMGSAVN DALKRDKDAI YGHPLFPLLA LVFEKCELAT
110 120 130 140 150
CTPREPGVAG GDVCSSDSFN EDIAVFAKQV RAEKPLFSSN PELDNLMIQA
160 170 180 190 200
IQVLRFHLLE LEKVHELCDN FCHRYISCLK GKMPIDLVID ERDGSSKSDH
210 220 230 240 250
EELSGSSTNL ADHNPSSWRD HDDATSTHSA GTPGPSSGGH ASQSGDNSSE
260 270 280 290 300
QGDGLDNSVA SPGTGDDDDP DKDKKRQKKR GIFPKVATNI MRAWLFQHLT
310 320 330 340 350
HPYPSEEQKK QLAQDTGLTI LQVNNWFINA RRRIVQPMID QSNRAGFLLD
360 370 380 390 400
PSVSQGAAYS PEGQPMGSFV LDGQQHMGIR PAGLQSMPGD YVSQGGPMGM
410 420 430 440 450
GMAQPSYTPP QMTPHPTQLR HGPPMHSYLP SHPHHPAMVM HGGPPTHPGM
460 470
TMSAQSPTML NSVDPNVGGQ VMDIHAQ
Length:477
Mass (Da):51,728
Last modified:December 15, 1998 - v2
Checksum:i923610800B647A21
GO
Isoform Meis2A (identifier: P97367-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     384-401: LQSMPGDYVSQGGPMGMG → PMSGMGMNMGMDGQWHYM
     402-477: Missing.

Show »
Length:401
Mass (Da):43,791
Checksum:i0682C0E92326516D
GO
Isoform Meis2B (identifier: P97367-3) [UniParc]FASTAAdd to Basket

Also known as: Mrg1A

The sequence of this isoform differs from the canonical sequence as follows:
     346-352: Missing.
     384-401: LQSMPGDYVSQGGPMGMG → PMSGMGMNMGMDGQWHYM
     402-477: Missing.

Show »
Length:394
Mass (Da):43,061
Checksum:i6BCC0382F0C55296
GO
Isoform Meis2D (identifier: P97367-4) [UniParc]FASTAAdd to Basket

Also known as: Mrg1B

The sequence of this isoform differs from the canonical sequence as follows:
     346-352: Missing.

Show »
Length:470
Mass (Da):50,998
Checksum:iC78142B9E15CDDBC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21A → E in AAC52948. (PubMed:8950991)Curated
Sequence conflicti316 – 3161T → A in AAC52948. (PubMed:8950991)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei346 – 3527Missing in isoform Meis2B and isoform Meis2D. 2 PublicationsVSP_002247
Alternative sequencei384 – 40118LQSMP…PMGMG → PMSGMGMNMGMDGQWHYM in isoform Meis2A and isoform Meis2B. 3 PublicationsVSP_002248Add
BLAST
Alternative sequencei402 – 47776Missing in isoform Meis2A and isoform Meis2B. 3 PublicationsVSP_002249Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57343 mRNA. Translation: AAC52948.1.
AJ000504 mRNA. Translation: CAA04138.1.
AJ000505 mRNA. Translation: CAA04139.1.
AJ000506 mRNA. Translation: CAA04140.1.
AJ000507 mRNA. Translation: CAA04141.1.
U68383 mRNA. Translation: AAB19193.1.
U68384 mRNA. Translation: AAB19194.1.
CCDSiCCDS16568.1. [P97367-4]
CCDS50666.1. [P97367-3]
CCDS50667.1. [P97367-1]
CCDS50668.1. [P97367-2]
RefSeqiNP_001129544.1. NM_001136072.2. [P97367-3]
NP_001153039.1. NM_001159567.1. [P97367-2]
NP_001153040.1. NM_001159568.1. [P97367-1]
NP_001153041.1. NM_001159569.1.
NP_034955.1. NM_010825.3. [P97367-4]
UniGeneiMm.247566.
Mm.471935.

Genome annotation databases

EnsembliENSMUST00000028639; ENSMUSP00000028639; ENSMUSG00000027210. [P97367-1]
ENSMUST00000102538; ENSMUSP00000099597; ENSMUSG00000027210. [P97367-4]
ENSMUST00000110907; ENSMUSP00000106532; ENSMUSG00000027210. [P97367-2]
ENSMUST00000110908; ENSMUSP00000106533; ENSMUSG00000027210. [P97367-3]
GeneIDi17536.
KEGGimmu:17536.
UCSCiuc008lqy.2. mouse. [P97367-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U57343 mRNA. Translation: AAC52948.1 .
AJ000504 mRNA. Translation: CAA04138.1 .
AJ000505 mRNA. Translation: CAA04139.1 .
AJ000506 mRNA. Translation: CAA04140.1 .
AJ000507 mRNA. Translation: CAA04141.1 .
U68383 mRNA. Translation: AAB19193.1 .
U68384 mRNA. Translation: AAB19194.1 .
CCDSi CCDS16568.1. [P97367-4 ]
CCDS50666.1. [P97367-3 ]
CCDS50667.1. [P97367-1 ]
CCDS50668.1. [P97367-2 ]
RefSeqi NP_001129544.1. NM_001136072.2. [P97367-3 ]
NP_001153039.1. NM_001159567.1. [P97367-2 ]
NP_001153040.1. NM_001159568.1. [P97367-1 ]
NP_001153041.1. NM_001159569.1.
NP_034955.1. NM_010825.3. [P97367-4 ]
UniGenei Mm.247566.
Mm.471935.

3D structure databases

ProteinModelPortali P97367.
SMRi P97367. Positions 283-338.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201487. 8 interactions.
IntActi P97367. 1 interaction.

PTM databases

PhosphoSitei P97367.

Proteomic databases

MaxQBi P97367.
PaxDbi P97367.
PRIDEi P97367.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000028639 ; ENSMUSP00000028639 ; ENSMUSG00000027210 . [P97367-1 ]
ENSMUST00000102538 ; ENSMUSP00000099597 ; ENSMUSG00000027210 . [P97367-4 ]
ENSMUST00000110907 ; ENSMUSP00000106532 ; ENSMUSG00000027210 . [P97367-2 ]
ENSMUST00000110908 ; ENSMUSP00000106533 ; ENSMUSG00000027210 . [P97367-3 ]
GeneIDi 17536.
KEGGi mmu:17536.
UCSCi uc008lqy.2. mouse. [P97367-1 ]

Organism-specific databases

CTDi 4212.
MGIi MGI:108564. Meis2.

Phylogenomic databases

eggNOGi NOG302919.
GeneTreei ENSGT00550000074260.
HOGENOMi HOG000253923.
HOVERGENi HBG055193.
InParanoidi P97367.
KOi K16670.
OMAi SQHYGAH.
OrthoDBi EOG76HQ2W.
PhylomeDBi P97367.
TreeFami TF318093.

Miscellaneous databases

NextBioi 292164.
PROi P97367.
SOURCEi Search...

Gene expression databases

Bgeei P97367.
CleanExi MM_MEIS2.
ExpressionAtlasi P97367. baseline and differential.
Genevestigatori P97367.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
InterProi IPR001356. Homeobox_dom.
IPR008422. Homeobox_KN_domain.
IPR009057. Homeodomain-like.
[Graphical view ]
Pfami PF05920. Homeobox_KN. 1 hit.
[Graphical view ]
SMARTi SM00389. HOX. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
PROSITEi PS50071. HOMEOBOX_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Identification of a new family of Pbx-related homeobox genes."
    Nakamura T., Jenkins N.A., Copeland N.G.
    Oncogene 13:2235-2242(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MEIS2A).
    Strain: Swiss Webster.
  2. "Meis2, a novel mouse Pbx-related homeobox gene induced by retinoic acid during differentiation of P19 embryonal carcinoma cells."
    Oulad-Abdelghani M., Chazaud C., Bouillet P., Sapin V., Chambon P., Dolle P.
    Dev. Dyn. 210:173-183(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MEIS2A; MEIS2B; MEIS2C AND MEIS2D).
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS MEIS2B AND MEIS2D).
  4. "An endocrine-exocrine switch in the activity of the pancreatic homeodomain protein PDX1 through formation of a trimeric complex with PBX1b and MRG1 (MEIS2)."
    Swift G.H., Liu Y., Rose S.D., Bischof L.J., Steelman S., Buchberg A.M., Wright C.V., MacDonald R.J.
    Mol. Cell. Biol. 18:5109-5120(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN A COMPLEX WITH PDX1 AND PBX1.
  5. "Expression of Meis and Pbx genes and their protein products in the developing telencephalon: implications for regional differentiation."
    Toresson H., Parmar M., Campbell K.
    Mech. Dev. 94:183-187(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. "Inhibition of myeloid differentiation by Hoxa9, Hoxb8, and Meis homeobox genes."
    Fujino T., Yamazaki Y., Largaespada D.A., Jenkins N.A., Copeland N.G., Hirokawa K., Nakamura T.
    Exp. Hematol. 29:856-863(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  7. "DNA binding and transcriptional activation by a PDX1.PBX1b.MEIS2b trimer and cooperation with a pancreas-specific basic helix-loop-helix complex."
    Liu Y., MacDonald R.J., Swift G.H.
    J. Biol. Chem. 276:17985-17993(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PBX1, IDENTIFICATION IN A COMPLEX WITH PDX1 AND PBX1.
  8. "Range of HOX/TALE superclass associations and protein domain requirements for HOXA13:MEIS interaction."
    Williams T.M., Williams M.E., Innis J.W.
    Dev. Biol. 277:457-471(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HOXA13.
  9. "Regulation of EphA8 gene expression by TALE homeobox transcription factors during development of the mesencephalon."
    Shim S., Kim Y., Shin J., Kim J., Park S.
    Mol. Cell. Biol. 27:1614-1630(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.

Entry informationi

Entry nameiMEIS2_MOUSE
AccessioniPrimary (citable) accession number: P97367
Secondary accession number(s): O35676
, O35677, P97403, P97404
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: December 15, 1998
Last modified: October 29, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3