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P97363

- SPTC2_MOUSE

UniProt

P97363 - SPTC2_MOUSE

Protein

Serine palmitoyltransferase 2

Gene

Sptlc2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 2 (01 Jan 1998)
      Previous versions | rss
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    Functioni

    Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate By similarity.By similarity

    Catalytic activityi

    Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

    Cofactori

    Pyridoxal phosphate.By similarity

    Pathwayi

    GO - Molecular functioni

    1. pyridoxal phosphate binding Source: InterPro
    2. serine C-palmitoyltransferase activity Source: MGI

    GO - Biological processi

    1. ceramide biosynthetic process Source: MGI
    2. sphinganine biosynthetic process Source: MGI
    3. sphingomyelin biosynthetic process Source: MGI
    4. sphingosine biosynthetic process Source: MGI

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Lipid metabolism, Sphingolipid metabolism

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    ReactomeiREACT_198151. Sphingolipid de novo biosynthesis.
    UniPathwayiUPA00222.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine palmitoyltransferase 2 (EC:2.3.1.50)
    Alternative name(s):
    Long chain base biosynthesis protein 2
    Short name:
    LCB 2
    Long chain base biosynthesis protein 2a
    Short name:
    LCB2a
    Serine-palmitoyl-CoA transferase 2
    Short name:
    SPT 2
    Gene namesi
    Name:Sptlc2
    Synonyms:Lcb2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 12

    Organism-specific databases

    MGIiMGI:108074. Sptlc2.

    Subcellular locationi

    Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. mitochondrion Source: MGI
    3. serine C-palmitoyltransferase complex Source: MGI

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 560560Serine palmitoyltransferase 2PRO_0000163859Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei377 – 3771N6-(pyridoxal phosphate)lysineBy similarity

    Proteomic databases

    MaxQBiP97363.
    PaxDbiP97363.
    PRIDEiP97363.

    PTM databases

    PhosphoSiteiP97363.

    Expressioni

    Tissue specificityi

    Expressed in a variety of tissues.

    Gene expression databases

    ArrayExpressiP97363.
    BgeeiP97363.
    GenevestigatoriP97363.

    Interactioni

    Subunit structurei

    Heterodimer with SPTLC1. Component of the serine palmitoyltransferase (SPT) complex, composed of LCB1/SPTLC1, LCB2 (SPTLC2 or SPTLC3) and ssPT (SPTSSA or SPTSSB) By similarity.By similarity

    Protein-protein interaction databases

    IntActiP97363. 1 interaction.
    MINTiMINT-4100293.

    Structurei

    3D structure databases

    ProteinModelPortaliP97363.
    SMRiP97363. Positions 110-533.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei65 – 8521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0156.
    HOGENOMiHOG000206826.
    HOVERGENiHBG002230.
    InParanoidiP97363.
    KOiK00654.
    OMAiPEPGGCC.
    OrthoDBiEOG73RBB0.
    PhylomeDBiP97363.
    TreeFamiTF300452.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PfamiPF00155. Aminotran_1_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97363-1 [UniParc]FASTAAdd to Basket

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    MRPEPGGCCC RRPMRANGCV KNGEVRNGYL RSSTATVAAA GQIHHVTENG    50
    GLYKRPFNEA FEETPMLVAV LTYVGYGVLT LFGYLRDFLR HWRIEKCHHA 100
    TEREEQKDFV SLYQDFENFY TRNLYMRIRD NWNRPICSVP GAKVDIMERK 150
    SHDYNWSFKY TGNIIKGVIN MGSYNYLGFA RNTGSCQEAA AEVLKEYGAG 200
    VCSTRQEIGN LDKHEELEKL VARFLGVEAA MTYGMGFATN SMNIPALVGK 250
    GCLILSDELN HASLVLGARL SGATIRIFKH NNMQSLEKLL KDAIVYGQPR 300
    TRRPWKKILI LVEGIYSMEG SIVRLPEVIA LKKKYKAYLY LDEAHSIGAL 350
    GPSGRGVVDY FGLDPEDVDV MMGTFTKSFG ASGGYIGGKK ELIDYLRTHS 400
    HSAVYATSMS PPVMEQIITS MKCIMGQDGT SLGKECIQQL AENTRYFRRR 450
    LKEMGFIIYG NEDSPVVPLM LYMPAKIGAF GREMLKRNIG VVVVGFPATP 500
    IIESRARFCL SAAHTKEILD TALKEIDEVG DLLQLKYSRH RLVPLLDRPF 550
    DETTYEETED 560
    Length:560
    Mass (Da):62,982
    Last modified:January 1, 1998 - v2
    Checksum:i6429566979B18D1C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27455 mRNA. Translation: AAC53310.1.
    X95642 mRNA. Translation: CAA64898.1.
    BC003227 mRNA. Translation: AAH03227.1.
    CCDSiCCDS26076.1.
    PIRiJC5180.
    RefSeqiNP_035609.1. NM_011479.3.
    UniGeneiMm.565.

    Genome annotation databases

    EnsembliENSMUST00000021424; ENSMUSP00000021424; ENSMUSG00000021036.
    GeneIDi20773.
    KEGGimmu:20773.
    UCSCiuc007oiw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U27455 mRNA. Translation: AAC53310.1 .
    X95642 mRNA. Translation: CAA64898.1 .
    BC003227 mRNA. Translation: AAH03227.1 .
    CCDSi CCDS26076.1.
    PIRi JC5180.
    RefSeqi NP_035609.1. NM_011479.3.
    UniGenei Mm.565.

    3D structure databases

    ProteinModelPortali P97363.
    SMRi P97363. Positions 110-533.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P97363. 1 interaction.
    MINTi MINT-4100293.

    PTM databases

    PhosphoSitei P97363.

    Proteomic databases

    MaxQBi P97363.
    PaxDbi P97363.
    PRIDEi P97363.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000021424 ; ENSMUSP00000021424 ; ENSMUSG00000021036 .
    GeneIDi 20773.
    KEGGi mmu:20773.
    UCSCi uc007oiw.1. mouse.

    Organism-specific databases

    CTDi 9517.
    MGIi MGI:108074. Sptlc2.

    Phylogenomic databases

    eggNOGi COG0156.
    HOGENOMi HOG000206826.
    HOVERGENi HBG002230.
    InParanoidi P97363.
    KOi K00654.
    OMAi PEPGGCC.
    OrthoDBi EOG73RBB0.
    PhylomeDBi P97363.
    TreeFami TF300452.

    Enzyme and pathway databases

    UniPathwayi UPA00222 .
    Reactomei REACT_198151. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    ChiTaRSi SPTLC2. mouse.
    NextBioi 299487.
    PROi P97363.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97363.
    Bgeei P97363.
    Genevestigatori P97363.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR001917. Aminotrans_II_pyridoxalP_BS.
    IPR004839. Aminotransferase_I/II.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    Pfami PF00155. Aminotran_1_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sphingolipid synthesis: identification and characterization of mammalian cDNAs encoding the Lcb2 subunit of serine palmitoyltransferase."
      Nagiec M.M., Lester R.L., Dickson R.C.
      Gene 177:237-241(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney and Testis.
    2. Nagiec M.M., Lester R.L., Dickson R.C.
      Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Human and murine serine-palmitoyl-CoA transferase. Cloning, expression and characterization of the key enzyme in sphingolipid synthesis."
      Weiss B., Stoffel W.
      Eur. J. Biochem. 249:239-247(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Kidney and Liver.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    5. "Subcellular localization and membrane topology of serine palmitoyltransferase, 3-dehydrosphinganine reductase, and sphinganine N-acyltransferase in mouse liver."
      Mandon E.C., Ehses I., Rother J., van Echten G., Sandhoff K.
      J. Biol. Chem. 267:11144-11148(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Tissue: Liver.

    Entry informationi

    Entry nameiSPTC2_MOUSE
    AccessioniPrimary (citable) accession number: P97363
    Secondary accession number(s): P97356
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: January 1, 1998
    Last modified: October 1, 2014
    This is version 123 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3