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P97363

- SPTC2_MOUSE

UniProt

P97363 - SPTC2_MOUSE

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Protein

Serine palmitoyltransferase 2

Gene

Sptlc2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate (By similarity).By similarity

Catalytic activityi

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactori

Pathwayi

GO - Molecular functioni

  1. pyridoxal phosphate binding Source: InterPro
  2. serine C-palmitoyltransferase activity Source: MGI

GO - Biological processi

  1. ceramide biosynthetic process Source: MGI
  2. sphinganine biosynthetic process Source: MGI
  3. sphingomyelin biosynthetic process Source: MGI
  4. sphingosine biosynthetic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid metabolism, Sphingolipid metabolism

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

ReactomeiREACT_198151. Sphingolipid de novo biosynthesis.
UniPathwayiUPA00222.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine palmitoyltransferase 2 (EC:2.3.1.50)
Alternative name(s):
Long chain base biosynthesis protein 2
Short name:
LCB 2
Long chain base biosynthesis protein 2a
Short name:
LCB2a
Serine-palmitoyl-CoA transferase 2
Short name:
SPT 2
Gene namesi
Name:Sptlc2
Synonyms:Lcb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:108074. Sptlc2.

Subcellular locationi

Endoplasmic reticulum membrane 1 Publication; Single-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei65 – 8521HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrion Source: MGI
  3. serine C-palmitoyltransferase complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 560560Serine palmitoyltransferase 2PRO_0000163859Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei377 – 3771N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

MaxQBiP97363.
PaxDbiP97363.
PRIDEiP97363.

PTM databases

PhosphoSiteiP97363.

Expressioni

Tissue specificityi

Expressed in a variety of tissues.

Gene expression databases

BgeeiP97363.
ExpressionAtlasiP97363. baseline and differential.
GenevestigatoriP97363.

Interactioni

Subunit structurei

Heterodimer with SPTLC1. Component of the serine palmitoyltransferase (SPT) complex, composed of LCB1/SPTLC1, LCB2 (SPTLC2 or SPTLC3) and ssPT (SPTSSA or SPTSSB) (By similarity).By similarity

Protein-protein interaction databases

IntActiP97363. 1 interaction.
MINTiMINT-4100293.

Structurei

3D structure databases

ProteinModelPortaliP97363.
SMRiP97363. Positions 110-533.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0156.
HOGENOMiHOG000206826.
HOVERGENiHBG002230.
InParanoidiP97363.
KOiK00654.
OMAiPEPGGCC.
OrthoDBiEOG73RBB0.
PhylomeDBiP97363.
TreeFamiTF300452.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PfamiPF00155. Aminotran_1_2. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97363-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRPEPGGCCC RRPMRANGCV KNGEVRNGYL RSSTATVAAA GQIHHVTENG
60 70 80 90 100
GLYKRPFNEA FEETPMLVAV LTYVGYGVLT LFGYLRDFLR HWRIEKCHHA
110 120 130 140 150
TEREEQKDFV SLYQDFENFY TRNLYMRIRD NWNRPICSVP GAKVDIMERK
160 170 180 190 200
SHDYNWSFKY TGNIIKGVIN MGSYNYLGFA RNTGSCQEAA AEVLKEYGAG
210 220 230 240 250
VCSTRQEIGN LDKHEELEKL VARFLGVEAA MTYGMGFATN SMNIPALVGK
260 270 280 290 300
GCLILSDELN HASLVLGARL SGATIRIFKH NNMQSLEKLL KDAIVYGQPR
310 320 330 340 350
TRRPWKKILI LVEGIYSMEG SIVRLPEVIA LKKKYKAYLY LDEAHSIGAL
360 370 380 390 400
GPSGRGVVDY FGLDPEDVDV MMGTFTKSFG ASGGYIGGKK ELIDYLRTHS
410 420 430 440 450
HSAVYATSMS PPVMEQIITS MKCIMGQDGT SLGKECIQQL AENTRYFRRR
460 470 480 490 500
LKEMGFIIYG NEDSPVVPLM LYMPAKIGAF GREMLKRNIG VVVVGFPATP
510 520 530 540 550
IIESRARFCL SAAHTKEILD TALKEIDEVG DLLQLKYSRH RLVPLLDRPF
560
DETTYEETED
Length:560
Mass (Da):62,982
Last modified:January 1, 1998 - v2
Checksum:i6429566979B18D1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27455 mRNA. Translation: AAC53310.1.
X95642 mRNA. Translation: CAA64898.1.
BC003227 mRNA. Translation: AAH03227.1.
CCDSiCCDS26076.1.
PIRiJC5180.
RefSeqiNP_035609.1. NM_011479.3.
UniGeneiMm.565.

Genome annotation databases

EnsembliENSMUST00000021424; ENSMUSP00000021424; ENSMUSG00000021036.
GeneIDi20773.
KEGGimmu:20773.
UCSCiuc007oiw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27455 mRNA. Translation: AAC53310.1 .
X95642 mRNA. Translation: CAA64898.1 .
BC003227 mRNA. Translation: AAH03227.1 .
CCDSi CCDS26076.1.
PIRi JC5180.
RefSeqi NP_035609.1. NM_011479.3.
UniGenei Mm.565.

3D structure databases

ProteinModelPortali P97363.
SMRi P97363. Positions 110-533.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97363. 1 interaction.
MINTi MINT-4100293.

PTM databases

PhosphoSitei P97363.

Proteomic databases

MaxQBi P97363.
PaxDbi P97363.
PRIDEi P97363.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000021424 ; ENSMUSP00000021424 ; ENSMUSG00000021036 .
GeneIDi 20773.
KEGGi mmu:20773.
UCSCi uc007oiw.1. mouse.

Organism-specific databases

CTDi 9517.
MGIi MGI:108074. Sptlc2.

Phylogenomic databases

eggNOGi COG0156.
HOGENOMi HOG000206826.
HOVERGENi HBG002230.
InParanoidi P97363.
KOi K00654.
OMAi PEPGGCC.
OrthoDBi EOG73RBB0.
PhylomeDBi P97363.
TreeFami TF300452.

Enzyme and pathway databases

UniPathwayi UPA00222 .
Reactomei REACT_198151. Sphingolipid de novo biosynthesis.

Miscellaneous databases

NextBioi 299487.
PROi P97363.
SOURCEi Search...

Gene expression databases

Bgeei P97363.
ExpressionAtlasi P97363. baseline and differential.
Genevestigatori P97363.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR001917. Aminotrans_II_pyridoxalP_BS.
IPR004839. Aminotransferase_I/II.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view ]
Pfami PF00155. Aminotran_1_2. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00599. AA_TRANSFER_CLASS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sphingolipid synthesis: identification and characterization of mammalian cDNAs encoding the Lcb2 subunit of serine palmitoyltransferase."
    Nagiec M.M., Lester R.L., Dickson R.C.
    Gene 177:237-241(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney and Testis.
  2. Nagiec M.M., Lester R.L., Dickson R.C.
    Submitted (JAN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Human and murine serine-palmitoyl-CoA transferase. Cloning, expression and characterization of the key enzyme in sphingolipid synthesis."
    Weiss B., Stoffel W.
    Eur. J. Biochem. 249:239-247(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Kidney and Liver.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  5. "Subcellular localization and membrane topology of serine palmitoyltransferase, 3-dehydrosphinganine reductase, and sphinganine N-acyltransferase in mouse liver."
    Mandon E.C., Ehses I., Rother J., van Echten G., Sandhoff K.
    J. Biol. Chem. 267:11144-11148(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Tissue: Liver.

Entry informationi

Entry nameiSPTC2_MOUSE
AccessioniPrimary (citable) accession number: P97363
Secondary accession number(s): P97356
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: November 26, 2014
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3