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Protein

Serine palmitoyltransferase 2

Gene

Sptlc2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate (By similarity).By similarity

Catalytic activityi

Palmitoyl-CoA + L-serine = CoA + 3-dehydro-D-sphinganine + CO2.

Cofactori

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

GO - Molecular functioni

GO - Biological processi

  • ceramide biosynthetic process Source: MGI
  • positive regulation of lipophagy Source: MGI
  • sphinganine biosynthetic process Source: MGI
  • sphingolipid biosynthetic process Source: MGI
  • sphingomyelin biosynthetic process Source: MGI
  • sphingosine biosynthetic process Source: MGI

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processLipid metabolism, Sphingolipid metabolism
LigandPyridoxal phosphate

Enzyme and pathway databases

ReactomeiR-MMU-1660661 Sphingolipid de novo biosynthesis
UniPathwayiUPA00222

Names & Taxonomyi

Protein namesi
Recommended name:
Serine palmitoyltransferase 2 (EC:2.3.1.50)
Alternative name(s):
Long chain base biosynthesis protein 2
Short name:
LCB 2
Long chain base biosynthesis protein 2a
Short name:
LCB2a
Serine-palmitoyl-CoA transferase 2
Short name:
SPT 2
Gene namesi
Name:Sptlc2
Synonyms:Lcb2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi

Organism-specific databases

MGIiMGI:108074 Sptlc2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei65 – 85HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001638591 – 560Serine palmitoyltransferase 2Add BLAST560

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei377N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

EPDiP97363
PaxDbiP97363
PeptideAtlasiP97363
PRIDEiP97363

PTM databases

iPTMnetiP97363
PhosphoSitePlusiP97363

Expressioni

Tissue specificityi

Expressed in a variety of tissues.

Gene expression databases

BgeeiENSMUSG00000021036
ExpressionAtlasiP97363 baseline and differential
GenevisibleiP97363 MM

Interactioni

Subunit structurei

Heterodimer with SPTLC1. Component of the serine palmitoyltransferase (SPT) complex, composed of LCB1/SPTLC1, LCB2 (SPTLC2 or SPTLC3) and ssPT (SPTSSA or SPTSSB) (By similarity).By similarity

Protein-protein interaction databases

BioGridi203487, 12 interactors
IntActiP97363, 13 interactors
MINTiP97363
STRINGi10090.ENSMUSP00000021424

Structurei

3D structure databases

ProteinModelPortaliP97363
SMRiP97363
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1357 Eukaryota
COG0156 LUCA
GeneTreeiENSGT00550000074678
HOGENOMiHOG000206826
HOVERGENiHBG002230
InParanoidiP97363
KOiK00654
OMAiTLNMSSY
OrthoDBiEOG091G0558
PhylomeDBiP97363
TreeFamiTF300452

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR001917 Aminotrans_II_pyridoxalP_BS
IPR004839 Aminotransferase_I/II
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00599 AA_TRANSFER_CLASS_2, 1 hit

Sequencei

Sequence statusi: Complete.

P97363-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPEPGGCCC RRPMRANGCV KNGEVRNGYL RSSTATVAAA GQIHHVTENG
60 70 80 90 100
GLYKRPFNEA FEETPMLVAV LTYVGYGVLT LFGYLRDFLR HWRIEKCHHA
110 120 130 140 150
TEREEQKDFV SLYQDFENFY TRNLYMRIRD NWNRPICSVP GAKVDIMERK
160 170 180 190 200
SHDYNWSFKY TGNIIKGVIN MGSYNYLGFA RNTGSCQEAA AEVLKEYGAG
210 220 230 240 250
VCSTRQEIGN LDKHEELEKL VARFLGVEAA MTYGMGFATN SMNIPALVGK
260 270 280 290 300
GCLILSDELN HASLVLGARL SGATIRIFKH NNMQSLEKLL KDAIVYGQPR
310 320 330 340 350
TRRPWKKILI LVEGIYSMEG SIVRLPEVIA LKKKYKAYLY LDEAHSIGAL
360 370 380 390 400
GPSGRGVVDY FGLDPEDVDV MMGTFTKSFG ASGGYIGGKK ELIDYLRTHS
410 420 430 440 450
HSAVYATSMS PPVMEQIITS MKCIMGQDGT SLGKECIQQL AENTRYFRRR
460 470 480 490 500
LKEMGFIIYG NEDSPVVPLM LYMPAKIGAF GREMLKRNIG VVVVGFPATP
510 520 530 540 550
IIESRARFCL SAAHTKEILD TALKEIDEVG DLLQLKYSRH RLVPLLDRPF
560
DETTYEETED
Length:560
Mass (Da):62,982
Last modified:January 1, 1998 - v2
Checksum:i6429566979B18D1C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U27455 mRNA Translation: AAC53310.1
X95642 mRNA Translation: CAA64898.1
BC003227 mRNA Translation: AAH03227.1
CCDSiCCDS26076.1
PIRiJC5180
RefSeqiNP_035609.1, NM_011479.4
UniGeneiMm.440577
Mm.565

Genome annotation databases

EnsembliENSMUST00000021424; ENSMUSP00000021424; ENSMUSG00000021036
GeneIDi20773
KEGGimmu:20773
UCSCiuc007oiw.1 mouse

Entry informationi

Entry nameiSPTC2_MOUSE
AccessioniPrimary (citable) accession number: P97363
Secondary accession number(s): P97356
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1998
Last modified: May 23, 2018
This is version 151 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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