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Protein

BPI fold-containing family A member 1

Gene

Bpifa1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the innate immune responses of the upper airways. Reduces the surface tension in secretions from airway epithelia and inhibits the formation of biofilm by pathogenic Gram-negative bacteria, such as P.aeruginosa and K.pneumoniae. Binds bacterial lipopolysaccharide (LPS). Negatively regulates proteolytic cleavage of SCNN1G, an event that is required for activation of the epithelial sodium channel (ENaC), and thereby contributes to airway surface liquid homeostasis and proper clearance of mucus (By similarity). Plays a role in the airway inflammatory response after exposure to irritants. May attract macrophages and neutrophils. May be associated with tumor progression.By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

  • antibacterial humoral response Source: UniProtKB
  • innate immune response Source: UniProtKB
  • multicellular organismal water homeostasis Source: UniProtKB
  • negative regulation of single-species biofilm formation in or on host organism Source: UniProtKB
  • regulation of liquid surface tension Source: UniProtKB
  • regulation of sodium ion transmembrane transport Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
BPI fold-containing family A member 1
Alternative name(s):
Palate lung and nasal epithelium clone protein
Gene namesi
Name:Bpifa1
Synonyms:Plunc, Splunc1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1338036. Bpifa1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 278259BPI fold-containing family A member 1PRO_0000017176Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi182 – 1821N-linked (GlcNAc...)Sequence analysis
Disulfide bondi204 ↔ 246By similarity
Glycosylationi228 – 2281N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP97361.
PaxDbiP97361.
PRIDEiP97361.

PTM databases

PhosphoSiteiP97361.

Expressioni

Tissue specificityi

Detected in airway epitelia (trachea and lung) and in bronchoalveolar fluid (at protein level). Upper airways, nasopharyngeal epithelium and thymus. Highest expression in the trachea and progressive decrease from proximal (bronchial) to distal (bronchiolar) airways. No expression is detected in the terminal bronchioles, respiratory bronchioles or lung alveoli.3 Publications

Developmental stagei

First detected at 14.5 dpc in the nasopharyngeal epithelium and persists there into adulthood. In the thymus, weak expression is detected at 16.5 dpc and appears to be restricted to epithelial cells lining the medullary venules. This pattern of thymic expression persists until birth and into early postnatal life but is greatly decreased in the adult thymus. No expression is detected in the lung until 2 days after birth, after which expression is detected in cells lining the trachea.1 Publication

Gene expression databases

BgeeiP97361.
CleanExiMM_PLUNC.
GenevisibleiP97361. MM.

Interactioni

Subunit structurei

Monomer. Interacts (via N-terminus) with SCNN1B, a subunit of the heterotrimeric epithelial sodium channel (ENaC); this inhibits proteolytic activation of ENaC (By similarity).By similarity

Protein-protein interaction databases

IntActiP97361. 1 interaction.
MINTiMINT-4108002.
STRINGi10090.ENSMUSP00000028985.

Structurei

3D structure databases

ProteinModelPortaliP97361.
SMRiP97361. Positions 72-278.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati23 – 286Repeat 1
Repeati30 – 367Repeat 2
Repeati39 – 446Repeat 3
Repeati47 – 526Repeat 4

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 52304 X 6 AA repeats of G-[LPQ]-[PL]-L-P-LAdd
BLAST

Sequence similaritiesi

Belongs to the BPI/LBP/Plunc superfamily. Plunc family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IWYF. Eukaryota.
ENOG41114MD. LUCA.
GeneTreeiENSGT00840000129898.
HOGENOMiHOG000231510.
HOVERGENiHBG045573.
InParanoidiP97361.
OMAiQGKVCPL.
OrthoDBiEOG7PZRZW.
PhylomeDBiP97361.
TreeFamiTF337052.

Family and domain databases

InterProiIPR017943. Bactericidal_perm-incr_a/b_dom.
IPR032942. BPI/LBP/Plunc.
IPR032953. BPIFA1/Latherin.
IPR017942. Lipid-bd_serum_glycop_N.
[Graphical view]
PANTHERiPTHR10504. PTHR10504. 2 hits.
PTHR10504:SF70. PTHR10504:SF70. 2 hits.
PfamiPF01273. LBP_BPI_CETP. 1 hit.
[Graphical view]
SUPFAMiSSF55394. SSF55394. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97361-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFLVGSLVVL CGLLAHSTAQ LAGLPLPLGQ GPPLPLNQGP PLPLNQGQLL
60 70 80 90 100
PLAQGLPLAV SPALPSNPTD LLAGKFTDAL SGGLLSGGLL GILENIPLLD
110 120 130 140 150
VIKSGGGNSN GLVGGLLGKL TSSVPLLNNI LDIKITDPQL LELGLVQSPD
160 170 180 190 200
GHRLYVTIPL GLTLNVNMPV VGSLLQLAVK LNITAEVLAV KDNQGRIHLV
210 220 230 240 250
LGDCTHSPGS LKISLLNGVT PVQSFLDNLT GILTKVLPEL IQGKVCPLVN
260 270
GILSGLDVTL VHNIAELLIH GLQFVIKV
Length:278
Mass (Da):28,625
Last modified:April 26, 2004 - v3
Checksum:i2540BED994475387
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti226 – 2261L → V (PubMed:10224143).Curated
Sequence conflicti226 – 2261L → V (PubMed:11396972).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69172 mRNA. Translation: AAB63256.1.
AF356785 Genomic DNA. Translation: AAK63069.1.
BC054375 mRNA. Translation: AAH54375.1.
CCDSiCCDS16925.1.
RefSeqiNP_035256.2. NM_011126.3.
UniGeneiMm.268852.

Genome annotation databases

EnsembliENSMUST00000028985; ENSMUSP00000028985; ENSMUSG00000027483.
GeneIDi18843.
KEGGimmu:18843.
UCSCiuc008niu.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U69172 mRNA. Translation: AAB63256.1.
AF356785 Genomic DNA. Translation: AAK63069.1.
BC054375 mRNA. Translation: AAH54375.1.
CCDSiCCDS16925.1.
RefSeqiNP_035256.2. NM_011126.3.
UniGeneiMm.268852.

3D structure databases

ProteinModelPortaliP97361.
SMRiP97361. Positions 72-278.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP97361. 1 interaction.
MINTiMINT-4108002.
STRINGi10090.ENSMUSP00000028985.

PTM databases

PhosphoSiteiP97361.

Proteomic databases

MaxQBiP97361.
PaxDbiP97361.
PRIDEiP97361.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000028985; ENSMUSP00000028985; ENSMUSG00000027483.
GeneIDi18843.
KEGGimmu:18843.
UCSCiuc008niu.2. mouse.

Organism-specific databases

CTDi51297.
MGIiMGI:1338036. Bpifa1.

Phylogenomic databases

eggNOGiENOG410IWYF. Eukaryota.
ENOG41114MD. LUCA.
GeneTreeiENSGT00840000129898.
HOGENOMiHOG000231510.
HOVERGENiHBG045573.
InParanoidiP97361.
OMAiQGKVCPL.
OrthoDBiEOG7PZRZW.
PhylomeDBiP97361.
TreeFamiTF337052.

Miscellaneous databases

PROiP97361.
SOURCEiSearch...

Gene expression databases

BgeeiP97361.
CleanExiMM_PLUNC.
GenevisibleiP97361. MM.

Family and domain databases

InterProiIPR017943. Bactericidal_perm-incr_a/b_dom.
IPR032942. BPI/LBP/Plunc.
IPR032953. BPIFA1/Latherin.
IPR017942. Lipid-bd_serum_glycop_N.
[Graphical view]
PANTHERiPTHR10504. PTHR10504. 2 hits.
PTHR10504:SF70. PTHR10504:SF70. 2 hits.
PfamiPF01273. LBP_BPI_CETP. 1 hit.
[Graphical view]
SUPFAMiSSF55394. SSF55394. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Differential display identification of plunc, a novel gene expressed in embryonic palate, nasal epithelium, and adult lung."
    Weston W.M., LeClair E.E., Trzyna W., McHugh K.M., Nugent P., Lafferty C.M., Ma L., Tuan R.S., Greene R.M.
    J. Biol. Chem. 274:13698-13703(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: ICR.
    Tissue: Palate.
  2. "Genomic organization of the mouse plunc gene and expression in the developing airways and thymus."
    LeClair E.E., Nguyen L., Bingle L., MacGowan A., Singleton V., Ward S.J., Bingle C.D.
    Biochem. Biophys. Res. Commun. 284:792-797(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: 129/Sv.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Olfactory epithelium.
  4. "SPLUNC1/BPIFA1 contributes to pulmonary host defense against Klebsiella pneumoniae respiratory infection."
    Liu Y., Bartlett J.A., Di M.E., Bomberger J.M., Chan Y.R., Gakhar L., Mallampalli R.K., McCray P.B. Jr., Di Y.P.
    Am. J. Pathol. 182:1519-1531(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.

Entry informationi

Entry nameiBPIA1_MOUSE
AccessioniPrimary (citable) accession number: P97361
Secondary accession number(s): Q7TQJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: April 26, 2004
Last modified: June 8, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.