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P97358 (TAF1B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 95. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
TATA box-binding protein-associated factor RNA polymerase I subunit B
Alternative name(s):
RNA polymerase I-specific TBP-associated factor 68 kDa
Short name=TAFI68
TATA box-binding protein-associated factor 1B
Short name=TBP-associated factor 1B
Transcription initiation factor SL1/TIF-IB subunit B
Gene names
Name:Taf1b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length586 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3. Ref.1

Subunit structure

Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with TBP and RRN3. Interacts with FLNA (via N-terminus). Ref.1 Ref.5 Ref.6

Subcellular location

Nucleusnucleolus By similarity.

Domain

Although it shares weak sequence similarity with GTF2B/TFIIB, displays a similar subdomain organization as GTF2B/TFIIB, with a N-terminal zinc finger, a connecting region (composed of B-reader and B-linker regions), followed by 2 cyclin folds. The RRN7-type zinc finger plays an essential postrecruitment role in Pol I transcription at a step preceding synthesis of the first 40 nucleotides By similarity.

Sequence similarities

Belongs to the RRN7/TAF1B family.

Contains 1 RRN7-type zinc finger.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97358-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97358-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.
     101-101: K → M
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 586586TATA box-binding protein-associated factor RNA polymerase I subunit B
PRO_0000304406

Regions

Zinc finger5 – 3935RRN7-type
Region40 – 6829B-reader By similarity
Region69 – 735B-linker By similarity
Region74 – 259186N-terminal cyclin fold By similarity
Region260 – 370111C-terminal cyclin fold By similarity

Sites

Metal binding131Zinc By similarity
Metal binding161Zinc By similarity
Metal binding311Zinc By similarity
Metal binding341Zinc By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue4381N6-acetyllysine By similarity

Natural variations

Alternative sequence1 – 100100Missing in isoform 2.
VSP_028024
Alternative sequence1011K → M in isoform 2.
VSP_028025

Experimental info

Sequence conflict21D → N in CAA71092. Ref.1
Sequence conflict21D → N in BAE38271. Ref.2
Sequence conflict21D → N in BAE41709. Ref.2
Sequence conflict21D → N in AAH57167. Ref.4
Sequence conflict21D → N in AAI25028. Ref.4
Sequence conflict21D → N in AAI25029. Ref.4
Sequence conflict1781K → T in AAI25029. Ref.4
Sequence conflict2871D → G in BAE20906. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 85E0EBB8C742EE71

FASTA58667,954
        10         20         30         40         50         60 
MDVEEVKAFR DRCSQCAAVS WGLTDEGKYY CTSCHNVTDR SEEVVSAADI PNTKINSINR 

        70         80         90        100        110        120 
GLRQRSKHEK GWDWYVCEGF QCILYHQAKA LETLGVSPEL KNEVLHNFWK RYLQKSKQAY 

       130        140        150        160        170        180 
CKNPVRTSGR KAKVLEDSVQ SSDLGSDLEL LSDTTCPLES EAEFQSDPQI PKPFPVTKGS 

       190        200        210        220        230        240 
PKSASVCSGS VDGVEYSERK EKGLVKMTVP RTLALCSLSL LWQRETITVS DLLRFVEEDH 

       250        260        270        280        290        300 
IPYINAFKLF PEEMKVYGRD KGIFAIESWP DYEDIYKNMI ELAIFLDLPR FPDITEDCYL 

       310        320        330        340        350        360 
HPNTLCMKYL LEVNLPDEMH TLTCQVVKLT GIGEVDFLTF DPIAKTKRTV KYDVQAMAVI 

       370        380        390        400        410        420 
VVVLKLLFLL DDKLEWSYSD LAEAYNEQHR EDTPQFDFRK WYQVMKKTFD EKRRKWEEAR 

       430        440        450        460        470        480 
ARYAWKTKRP LYRSHIDKSV AYKRRKMVEN LQKQFSALVG SSPVVEKQAP SSFQFNWTEE 

       490        500        510        520        530        540 
GTDSPCFHGH SLQGLLIMKG QSMITKNSLY WLSTQKFCKS YCKHVTTYEE SNFSLSYQFI 

       550        560        570        580 
LNIFSFLLRI KTSALHEEVS LLEKKLFEKK YNESKKSSGS KKGRRH 

« Hide

Isoform 2 [UniParc].

Checksum: 3306E944624DC8E1
Show »

FASTA48656,601

References

« Hide 'large scale' references
[1]"Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1."
Heix J., Zomerdijk J.C.B.M., Ravanpay A., Tjian R., Grummt I.
Proc. Natl. Acad. Sci. U.S.A. 94:1733-1738(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH TBP; TAF1A AND TAF1C.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: C3H, C57BL/6J and NOD.
Tissue: Brain, Dendritic cell and Embryonic stem cell.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Rrn3 phosphorylation is a regulatory checkpoint for ribosome biogenesis."
Cavanaugh A.H., Hirschler-Laszkiewicz I., Hu Q., Dundr M., Smink T., Misteli T., Rothblum L.I.
J. Biol. Chem. 277:27423-27432(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RRN3.
[6]"Identification of novel nuclear protein interactions with the N-terminal part of filamin A."
Qiu H., Nomiyama R., Moriguchi K., Fukada T., Sugimoto K.
Biosci. Biotechnol. Biochem. 75:145-147(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FLNA.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09973 Genomic DNA. Translation: CAA71092.1.
AK131963 mRNA. Translation: BAE20906.1.
AK165580 mRNA. Translation: BAE38271.1.
AK170313 mRNA. Translation: BAE41709.1.
AC125067 Genomic DNA. No translation available.
AC132587 Genomic DNA. No translation available.
BC057167 mRNA. Translation: AAH57167.1.
BC069907 mRNA. Translation: AAH69907.1.
BC094035 mRNA. Translation: AAH94035.1.
BC125027 mRNA. Translation: AAI25028.1.
BC125028 mRNA. Translation: AAI25029.1.
RefSeqNP_065639.2. NM_020614.2.
UniGeneMm.39082.
Mm.421402.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203957. 8 interactions.
IntActP97358. 1 interaction.
MINTMINT-249228.

PTM databases

PhosphoSiteP97358.

Proteomic databases

PRIDEP97358.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000075954; ENSMUSP00000075339; ENSMUSG00000059669. [P97358-1]
GeneID21340.
KEGGmmu:21340.
UCSCuc007nej.2. mouse. [P97358-1]
uc007nel.2. mouse. [P97358-2]

Organism-specific databases

CTD9014.
MGIMGI:109577. Taf1b.

Phylogenomic databases

eggNOGNOG72502.
GeneTreeENSGT00440000033827.
HOGENOMHOG000124571.
HOVERGENHBG053463.
InParanoidP97358.
KOK15213.
OMALSTQKFC.
OrthoDBEOG7V1FS7.
TreeFamTF324353.

Gene expression databases

BgeeP97358.
GenevestigatorP97358.

Family and domain databases

InterProIPR021752. TF_Rrn7.
[Graphical view]
PfamPF11781. RRN7. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio300516.
PROP97358.
SOURCESearch...

Entry information

Entry nameTAF1B_MOUSE
AccessionPrimary (citable) accession number: P97358
Secondary accession number(s): E9QJY7 expand/collapse secondary AC list , Q08AT7, Q3V292, Q6NSS9
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 27, 2011
Last modified: March 19, 2014
This is version 95 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot