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P97358

- TAF1B_MOUSE

UniProt

P97358 - TAF1B_MOUSE

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Protein
TATA box-binding protein-associated factor RNA polymerase I subunit B
Gene
Taf1b
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of RNA polymerase I core factor complex that acts as a GTF2B/TFIIB-like factor and plays a key role in multiple steps during trancription initiation such as preinitiation complex (PIC) assembly and postpolymerase recruitment events in polymerase I (Pol I) transcription. Binds rDNA promoters and plays a role in Pol I recruitment as a component of the SL1/TIF-IB complex and, possibly, directly through its interaction with RRN3.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi13 – 131Zinc By similarity
Metal bindingi16 – 161Zinc By similarity
Metal bindingi31 – 311Zinc By similarity
Metal bindingi34 – 341Zinc By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri5 – 3935RRN7-type
Add
BLAST

GO - Molecular functioni

  1. RNA polymerase I CORE element sequence-specific DNA binding Source: UniProtKB
  2. RNA polymerase I CORE element sequence-specific DNA binding transcription factor recruiting transcription factor activity Source: UniProtKB
  3. metal ion binding Source: UniProtKB-KW
  4. protein binding Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. RNA polymerase I transcriptional preinitiation complex assembly at the promoter for the nuclear large rRNA transcript Source: UniProtKB
  2. transcription from RNA polymerase I promoter Source: MGI
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_224328. SIRT1 negatively regulates rRNA Expression.

Names & Taxonomyi

Protein namesi
Recommended name:
TATA box-binding protein-associated factor RNA polymerase I subunit B
Alternative name(s):
RNA polymerase I-specific TBP-associated factor 68 kDa
Short name:
TAFI68
TATA box-binding protein-associated factor 1B
Short name:
TBP-associated factor 1B
Transcription initiation factor SL1/TIF-IB subunit B
Gene namesi
Name:Taf1b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 12

Organism-specific databases

MGIiMGI:109577. Taf1b.

Subcellular locationi

Nucleusnucleolus By similarity

GO - Cellular componenti

  1. RNA polymerase I core factor complex Source: Ensembl
  2. RNA polymerase I transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 586586TATA box-binding protein-associated factor RNA polymerase I subunit B
PRO_0000304406Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionine By similarity
Modified residuei438 – 4381N6-acetyllysine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP97358.

PTM databases

PhosphoSiteiP97358.

Expressioni

Gene expression databases

BgeeiP97358.
GenevestigatoriP97358.

Interactioni

Subunit structurei

Component of the transcription factor SL1/TIF-IB complex, composed of TBP and at least TAF1A, TAF1B, TAF1C and TAF1D. In the complex interacts directly with TBP, TAF1A and TAF1C. Interaction of the SL1/TIF-IB subunits with TBP excludes interaction of TBP with the transcription factor IID (TFIID) subunits. Interacts with TBP and RRN3. Interacts with FLNA (via N-terminus).3 Publications

Protein-protein interaction databases

BioGridi203957. 8 interactions.
IntActiP97358. 1 interaction.
MINTiMINT-249228.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni40 – 6829B-reader By similarity
Add
BLAST
Regioni69 – 735B-linker By similarity
Regioni74 – 259186N-terminal cyclin fold By similarity
Add
BLAST
Regioni260 – 370111C-terminal cyclin fold By similarity
Add
BLAST

Domaini

Although it shares weak sequence similarity with GTF2B/TFIIB, displays a similar subdomain organization as GTF2B/TFIIB, with a N-terminal zinc finger, a connecting region (composed of B-reader and B-linker regions), followed by 2 cyclin folds. The RRN7-type zinc finger plays an essential postrecruitment role in Pol I transcription at a step preceding synthesis of the first 40 nucleotides By similarity.

Sequence similaritiesi

Belongs to the RRN7/TAF1B family.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiNOG72502.
GeneTreeiENSGT00440000033827.
HOGENOMiHOG000124571.
HOVERGENiHBG053463.
InParanoidiP97358.
KOiK15213.
OMAiCFHGHSL.
OrthoDBiEOG7V1FS7.
TreeFamiTF324353.

Family and domain databases

InterProiIPR021752. TF_Rrn7.
[Graphical view]
PfamiPF11781. RRN7. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97358-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MDVEEVKAFR DRCSQCAAVS WGLTDEGKYY CTSCHNVTDR SEEVVSAADI    50
PNTKINSINR GLRQRSKHEK GWDWYVCEGF QCILYHQAKA LETLGVSPEL 100
KNEVLHNFWK RYLQKSKQAY CKNPVRTSGR KAKVLEDSVQ SSDLGSDLEL 150
LSDTTCPLES EAEFQSDPQI PKPFPVTKGS PKSASVCSGS VDGVEYSERK 200
EKGLVKMTVP RTLALCSLSL LWQRETITVS DLLRFVEEDH IPYINAFKLF 250
PEEMKVYGRD KGIFAIESWP DYEDIYKNMI ELAIFLDLPR FPDITEDCYL 300
HPNTLCMKYL LEVNLPDEMH TLTCQVVKLT GIGEVDFLTF DPIAKTKRTV 350
KYDVQAMAVI VVVLKLLFLL DDKLEWSYSD LAEAYNEQHR EDTPQFDFRK 400
WYQVMKKTFD EKRRKWEEAR ARYAWKTKRP LYRSHIDKSV AYKRRKMVEN 450
LQKQFSALVG SSPVVEKQAP SSFQFNWTEE GTDSPCFHGH SLQGLLIMKG 500
QSMITKNSLY WLSTQKFCKS YCKHVTTYEE SNFSLSYQFI LNIFSFLLRI 550
KTSALHEEVS LLEKKLFEKK YNESKKSSGS KKGRRH 586
Length:586
Mass (Da):67,954
Last modified:July 27, 2011 - v2
Checksum:i85E0EBB8C742EE71
GO
Isoform 2 (identifier: P97358-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-100: Missing.
     101-101: K → M

Note: No experimental confirmation available.

Show »
Length:486
Mass (Da):56,601
Checksum:i3306E944624DC8E1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 100100Missing in isoform 2.
VSP_028024Add
BLAST
Alternative sequencei101 – 1011K → M in isoform 2.
VSP_028025

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti2 – 21D → N in CAA71092. 1 Publication
Sequence conflicti2 – 21D → N in BAE38271. 1 Publication
Sequence conflicti2 – 21D → N in BAE41709. 1 Publication
Sequence conflicti2 – 21D → N in AAH57167. 1 Publication
Sequence conflicti2 – 21D → N in AAI25028. 1 Publication
Sequence conflicti2 – 21D → N in AAI25029. 1 Publication
Sequence conflicti178 – 1781K → T in AAI25029. 1 Publication
Sequence conflicti287 – 2871D → G in BAE20906. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09973 Genomic DNA. Translation: CAA71092.1.
AK131963 mRNA. Translation: BAE20906.1.
AK165580 mRNA. Translation: BAE38271.1.
AK170313 mRNA. Translation: BAE41709.1.
AC125067 Genomic DNA. No translation available.
AC132587 Genomic DNA. No translation available.
BC057167 mRNA. Translation: AAH57167.1.
BC069907 mRNA. Translation: AAH69907.1.
BC094035 mRNA. Translation: AAH94035.1.
BC125027 mRNA. Translation: AAI25028.1.
BC125028 mRNA. Translation: AAI25029.1.
CCDSiCCDS36419.1. [P97358-1]
RefSeqiNP_065639.2. NM_020614.2. [P97358-1]
UniGeneiMm.39082.
Mm.421402.

Genome annotation databases

EnsembliENSMUST00000075954; ENSMUSP00000075339; ENSMUSG00000059669. [P97358-1]
GeneIDi21340.
KEGGimmu:21340.
UCSCiuc007nej.2. mouse. [P97358-1]
uc007nel.2. mouse. [P97358-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09973 Genomic DNA. Translation: CAA71092.1 .
AK131963 mRNA. Translation: BAE20906.1 .
AK165580 mRNA. Translation: BAE38271.1 .
AK170313 mRNA. Translation: BAE41709.1 .
AC125067 Genomic DNA. No translation available.
AC132587 Genomic DNA. No translation available.
BC057167 mRNA. Translation: AAH57167.1 .
BC069907 mRNA. Translation: AAH69907.1 .
BC094035 mRNA. Translation: AAH94035.1 .
BC125027 mRNA. Translation: AAI25028.1 .
BC125028 mRNA. Translation: AAI25029.1 .
CCDSi CCDS36419.1. [P97358-1 ]
RefSeqi NP_065639.2. NM_020614.2. [P97358-1 ]
UniGenei Mm.39082.
Mm.421402.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

BioGridi 203957. 8 interactions.
IntActi P97358. 1 interaction.
MINTi MINT-249228.

PTM databases

PhosphoSitei P97358.

Proteomic databases

PRIDEi P97358.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000075954 ; ENSMUSP00000075339 ; ENSMUSG00000059669 . [P97358-1 ]
GeneIDi 21340.
KEGGi mmu:21340.
UCSCi uc007nej.2. mouse. [P97358-1 ]
uc007nel.2. mouse. [P97358-2 ]

Organism-specific databases

CTDi 9014.
MGIi MGI:109577. Taf1b.

Phylogenomic databases

eggNOGi NOG72502.
GeneTreei ENSGT00440000033827.
HOGENOMi HOG000124571.
HOVERGENi HBG053463.
InParanoidi P97358.
KOi K15213.
OMAi CFHGHSL.
OrthoDBi EOG7V1FS7.
TreeFami TF324353.

Enzyme and pathway databases

Reactomei REACT_224328. SIRT1 negatively regulates rRNA Expression.

Miscellaneous databases

NextBioi 300516.
PROi P97358.
SOURCEi Search...

Gene expression databases

Bgeei P97358.
Genevestigatori P97358.

Family and domain databases

InterProi IPR021752. TF_Rrn7.
[Graphical view ]
Pfami PF11781. RRN7. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of murine RNA polymerase I-specific TAF factors: conserved interactions between the subunits of the species-specific transcription initiation factor TIF-IB/SL1."
    Heix J., Zomerdijk J.C.B.M., Ravanpay A., Tjian R., Grummt I.
    Proc. Natl. Acad. Sci. U.S.A. 94:1733-1738(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, INTERACTION WITH TBP; TAF1A AND TAF1C.
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C3H, C57BL/6J and NOD.
    Tissue: Brain, Dendritic cell and Embryonic stem cell.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Rrn3 phosphorylation is a regulatory checkpoint for ribosome biogenesis."
    Cavanaugh A.H., Hirschler-Laszkiewicz I., Hu Q., Dundr M., Smink T., Misteli T., Rothblum L.I.
    J. Biol. Chem. 277:27423-27432(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RRN3.
  6. "Identification of novel nuclear protein interactions with the N-terminal part of filamin A."
    Qiu H., Nomiyama R., Moriguchi K., Fukada T., Sugimoto K.
    Biosci. Biotechnol. Biochem. 75:145-147(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FLNA.

Entry informationi

Entry nameiTAF1B_MOUSE
AccessioniPrimary (citable) accession number: P97358
Secondary accession number(s): E9QJY7
, Q08AT7, Q3V292, Q6NSS9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 100 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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