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P97355

- SPSY_MOUSE

UniProt

P97355 - SPSY_MOUSE

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Protein

Spermine synthase

Gene
Sms
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the production of spermine from spermidine and decarboxylated S-adenosylmethionine (dcSAM) By similarity. Required for normal viability, growth and fertility.1 Publication

Catalytic activityi

S-adenosyl 3-(methylthio)propylamine + spermidine = S-methyl-5'-thioadenosine + spermine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei148 – 1481S-adenosylmethioninamine By similarity
Binding sitei177 – 1771Spermidine By similarity
Binding sitei201 – 2011Spermidine By similarity
Binding sitei220 – 2201S-adenosylmethioninamine By similarity
Active sitei276 – 2761Proton acceptor By similarity
Binding sitei351 – 3511Spermidine By similarity
Binding sitei353 – 3531Spermidine By similarity

GO - Molecular functioni

  1. spermine synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. spermine biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Polyamine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00249; UER00315.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermine synthase (EC:2.5.1.22)
Short name:
SPMSY
Alternative name(s):
Spermidine aminopropyltransferase
Gene namesi
Name:Sms
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:109490. Sms.

Pathology & Biotechi

Disruption phenotypei

Mouse ES cells lacking Sms display normal growth rates but are sensitive to antiproliferative and DNA damage-inducing drugs.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 366365Spermine synthasePRO_0000156539Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP97355.
PaxDbiP97355.
PRIDEiP97355.

PTM databases

PhosphoSiteiP97355.

Expressioni

Gene expression databases

ArrayExpressiP97355.
BgeeiP97355.
CleanExiMM_SMS.
GenevestigatoriP97355.

Interactioni

Subunit structurei

Homodimer. Dimerization is mediated through the N-terminal domain and seems to be required for activity as deletion of the N-terminal domain causes complete loss of activity By similarity.

Protein-protein interaction databases

IntActiP97355. 1 interaction.
MINTiMINT-4135428.

Structurei

3D structure databases

ProteinModelPortaliP97355.
SMRiP97355. Positions 3-366.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini122 – 362241PABSAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni255 – 2562S-adenosylmethioninamine binding By similarity

Domaini

Composed of 3 domains: the N-terminal domain has structural similarity to S-adenosylmethionine decarboxylase, the central domain is made up of four beta strands and the C-terminal domain is similar in structure to spermidine synthase. The N- and C-terminal domains are both required for activity By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG259013.
GeneTreeiENSGT00620000088036.
HOVERGENiHBG004512.
InParanoidiP97355.
KOiK00802.
OMAiEGRMFDY.
OrthoDBiEOG7G1V6F.
PhylomeDBiP97355.
TreeFamiTF324508.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR001045. Spermidine/spermine_synthase.
IPR015576. Spermine_synthase_animal.
[Graphical view]
PANTHERiPTHR11558. PTHR11558. 1 hit.
PTHR11558:SF27. PTHR11558:SF27. 1 hit.
PfamiPF01564. Spermine_synth. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97355-1 [UniParc]FASTAAdd to Basket

« Hide

MAAARHSTLD FKLGAKADGE AILKGLQSIF QEQGMTESVH TWQDHGYLAT    50
YTNKNGSFAN LRIYPHGLVL LDLQSYDSDV QGKQETDSLL NKIEEKMKEL 100
SQDSTGRVKR LPPIVRGGAI DRYWPTADGR LVEYDIDEVV YDEDSPYQNI 150
KILHSKQFGN ILILSGDVNL AESDLAYTRA IMGSGKEDYT GKDVLILGGG 200
DGGILCEIVK LKPKMVTMVE IDQMVIDGCK KYMRRTCGDV LDNLRGDCYQ 250
VLIEDCIPVL KMYAKEGREF DYVINDLTAV PISTSPEEDS TWDFLRLILD 300
LSMKVLKQDG KYFTQGNCVN LTEALSLYEE QLGRLYCPVE FSKEIVCVPS 350
YLELWVFYTV WKKAKP 366
Length:366
Mass (Da):41,313
Last modified:May 1, 1997 - v1
Checksum:iD549F319F51C43C5
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti188 – 1881D → H in BAC33920. 1 Publication
Sequence conflicti245 – 2451R → K in BAC33920. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09419 mRNA. Translation: CAA70573.1.
AF031486 mRNA. Translation: AAB86631.1.
AK049787 mRNA. Translation: BAC33920.1.
AK011542 mRNA. Translation: BAB27685.1.
AK017775 mRNA. Translation: BAB30923.1.
AK149929 mRNA. Translation: BAE29173.1.
AK160637 mRNA. Translation: BAE35931.1.
AK160659 mRNA. Translation: BAE35947.1.
AK166665 mRNA. Translation: BAE38927.1.
AL663072 Genomic DNA. Translation: CAM18089.1.
BC046623 mRNA. Translation: AAH46623.1.
BC058688 mRNA. Translation: AAH58688.1.
AF136179 Genomic DNA. Translation: AAD33057.1.
AJ000093 Genomic DNA. Translation: CAA03919.1.
AJ000087
, AJ000088, AJ000089, AJ000090, AJ000091, AJ000092 Genomic DNA. Translation: CAA03918.1.
CCDSiCCDS41189.1.
RefSeqiNP_033240.3. NM_009214.3.
XP_001473484.1. XM_001473434.4.
XP_006544632.1. XM_006544569.1.
UniGeneiMm.18652.

Genome annotation databases

EnsembliENSMUST00000112529; ENSMUSP00000108148; ENSMUSG00000071708.
GeneIDi20603.
671878.
KEGGimmu:20603.
mmu:671878.
UCSCiuc009urz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09419 mRNA. Translation: CAA70573.1 .
AF031486 mRNA. Translation: AAB86631.1 .
AK049787 mRNA. Translation: BAC33920.1 .
AK011542 mRNA. Translation: BAB27685.1 .
AK017775 mRNA. Translation: BAB30923.1 .
AK149929 mRNA. Translation: BAE29173.1 .
AK160637 mRNA. Translation: BAE35931.1 .
AK160659 mRNA. Translation: BAE35947.1 .
AK166665 mRNA. Translation: BAE38927.1 .
AL663072 Genomic DNA. Translation: CAM18089.1 .
BC046623 mRNA. Translation: AAH46623.1 .
BC058688 mRNA. Translation: AAH58688.1 .
AF136179 Genomic DNA. Translation: AAD33057.1 .
AJ000093 Genomic DNA. Translation: CAA03919.1 .
AJ000087
, AJ000088 , AJ000089 , AJ000090 , AJ000091 , AJ000092 Genomic DNA. Translation: CAA03918.1 .
CCDSi CCDS41189.1.
RefSeqi NP_033240.3. NM_009214.3.
XP_001473484.1. XM_001473434.4.
XP_006544632.1. XM_006544569.1.
UniGenei Mm.18652.

3D structure databases

ProteinModelPortali P97355.
SMRi P97355. Positions 3-366.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97355. 1 interaction.
MINTi MINT-4135428.

PTM databases

PhosphoSitei P97355.

Proteomic databases

MaxQBi P97355.
PaxDbi P97355.
PRIDEi P97355.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000112529 ; ENSMUSP00000108148 ; ENSMUSG00000071708 .
GeneIDi 20603.
671878.
KEGGi mmu:20603.
mmu:671878.
UCSCi uc009urz.1. mouse.

Organism-specific databases

CTDi 6611.
MGIi MGI:109490. Sms.

Phylogenomic databases

eggNOGi NOG259013.
GeneTreei ENSGT00620000088036.
HOVERGENi HBG004512.
InParanoidi P97355.
KOi K00802.
OMAi EGRMFDY.
OrthoDBi EOG7G1V6F.
PhylomeDBi P97355.
TreeFami TF324508.

Enzyme and pathway databases

UniPathwayi UPA00249 ; UER00315 .

Miscellaneous databases

NextBioi 298937.
PROi P97355.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97355.
Bgeei P97355.
CleanExi MM_SMS.
Genevestigatori P97355.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR001045. Spermidine/spermine_synthase.
IPR015576. Spermine_synthase_animal.
[Graphical view ]
PANTHERi PTHR11558. PTHR11558. 1 hit.
PTHR11558:SF27. PTHR11558:SF27. 1 hit.
Pfami PF01564. Spermine_synth. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS01330. PABS_1. 1 hit.
PS51006. PABS_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Pex gene deletions in Gy and Hyp mice provide mouse models for X-linked hypophosphatemia."
    Strom T.M., Francis F., Lorenz B., Boeddrich A., Econs M.J., Lehrach H., Meitinger T.
    Hum. Mol. Genet. 6:165-171(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Nucleotide sequence of mouse spermidine aminopropyltransferase cDNA."
    Niiranen K., Korhonen V., Janne J.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Diaphragm.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow macrophage, Embryo, Embryonic spinal cord and Morula.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain and Olfactory epithelium.
  6. "Spermine deficiency resulting from targeted disruption of the spermine synthase gene in embryonic stem cells leads to enhanced sensitivity to antiproliferative drugs."
    Korhonen V.-P., Niiranen K., Halmekyto M., Pietila M., Diegelman P., Parkkinen J.J., Eloranta T., Porter C.W., Alhonen L., Janne J.
    Mol. Pharmacol. 59:231-238(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 58-315.
    Strain: 129/SvJ.
  7. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 297-307, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  8. "Spermine deficiency in Gy mice caused by deletion of the spermine synthase gene."
    Lorenz B., Francis F., Gempel K., Boeddrich A., Josten M., Schmahl W., Schmidt J., Lehrach H., Meitinger T., Strom T.M.
    Hum. Mol. Genet. 7:541-547(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 316-366, DISRUPTION PHENOTYPE.
  9. "Spermine synthesis is required for normal viability, growth, and fertility in the mouse."
    Wang X., Ikeguchi Y., McCloskey D.E., Nelson P., Pegg A.E.
    J. Biol. Chem. 279:51370-51375(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSPSY_MOUSE
AccessioniPrimary (citable) accession number: P97355
Secondary accession number(s): A2AC82
, Q3TL65, Q3TUP0, Q3UDT8, Q8C7P4, Q9CT09, Q9R282
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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