Reviewed, UniProtKB/Swiss-Prot P97353 (SEC1_MOUSE)

Names and origin

Protein names

Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 3
    EC=2.4.1.69
Alternative name(s):
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 3
    Alpha(1,2)FT 3
    Fucosyltransferase 10
    Secretory blood group protein 1

Gene names

Name:	Sec1
Synonyms:	Fut10

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	368 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Membrane-bound form in trans cisternae of Golgi By similarity.
Miscellaneous	In mouse, there are three genes (Fut1, Fut2 and Sec1) which encode galactoside 2-L-fucosyltransferase.
Sequence similarities	Belongs to the glycosyltransferase 11 family.
Caution	Was originally thought to be Fut2.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	protein amino acid glycosylation Ref.3 Inferred from direct assay. Source: UniProtKB
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Ref.3 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 368	368	Galactoside 2-alpha-L-fucosyltransferase 3		PRO_0000149115
Regions
	Topological domain	1 – 20	20	Cytoplasmic Potential
	Transmembrane	21 – 41	21	Signal-anchor for type II membrane protein Potential
	Topological domain	42 – 368	327	Lumenal Potential
Amino acid modifications
	Glycosylation	195	1	N-linked (GlcNAc...) Potential
	Glycosylation	289	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	46	1	G → S in strain: BFM/2Msf, C57BL/10SnJ and pgn2.
	Natural variant	50	1	A → G in strain: BFM/2Msf, C57BL/10SnJ and pgn2.
	Natural variant	62	1	P → A in strain: CAST/Ei.
	Natural variant	150	1	H → Q in strain: BFM/2Msf and C57BL/10SnJ.
	Natural variant	214	1	R → H in strain: pgn2.

Sequences

	Sequence		Length	Mass (Da)
	P97353-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 4093E853EB37303B	FASTA	368	41,464
	10 20 30 40 50 60 MPSDSCLLSL TVLQRLRAIC PPLSTFYLFF VIFVVSTIFH CHRRLGLVPA PWASPSLVVF 70 80 90 100 110 120 PPRHMPREGM FTIRVKGRLG NQMGEYATLF ALARMNGRLA FIPASMHSTL APIFRISLPV 130 140 150 160 170 180 LHSDTAKRIP WQNYHLNDWM EERYRHIPGH YVRFTGYPCS WTFYHHLRPE ILKEFTLHDH 190 200 210 220 230 240 VREEAQAFLR GLQVNGSQPS TFVGVHVRRG DYVRVMPKVW KGVVADRGYL EKALDRFRAR 250 260 270 280 290 300 YSSPVFVVTS DDMAWCRKSI TASRGDVAFA GNGLQGSPAK DIALLMQCNH TVITLGTFGI 310 320 330 340 350 360 WAAYLTGGDT VYLANFTQPN SPFHTVFKPE AAYLPEWVGI AADLGQPNTV GSGHASARAP KRHWGALL

References

[1]	"Molecular cloning and expression of a mouse GDP-L-Fucose: beta-D-galactoside 2-alpha-L-Fucosyltransferase." Hitoshi S., Kusunoki S., Kanazawa I., Tsuji S. Submitted (DEC-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ICR.
[2]	"GDP-fucose: beta-galactoside alpha1,2-fucosyltransferase, MFUT-II, and not MFUT-I or -III, is induced in a restricted region of the digestive tract of germ-free mice by host-microbe interactions and cycloheximide." Lin B., Hayashi Y., Saito M., Sakakibara Y., Yanagisawa M., Iwamori M. Biochim. Biophys. Acta 1487:275-285(2000) [PubMed: 11018479] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: ICR. Tissue: Gastrointestinal tract.
[3]	"Molecular cloning, genomic mapping, and expression of two secretor blood group alpha (1,2)fucosyltransferase genes differentially regulated in mouse uterine epithelium and gastrointestinal tract." Domino S.E., Zhang L., Lowe J.B. J. Biol. Chem. 276:23748-23756(2001) [PubMed: 11323419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Ola.
[4]	"Conspicuous differences among gene genealogies of 21 nuclear genes of five Mus musculus subspecies." Liu Y., Kitano T., Koide T., Shiroishi T., Moriwaki K., Saitou N. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: BFM/2Msf, BLG2/Msf, C57BL/10SnJ, CAST/Ei, HMI/Msf, MSM/Msf, NJL/Msf, pgn2 and SWN/Msf.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

Secretory blood group protein 1

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y09882 Genomic DNA. Translation: CAA71008.1. AF113532 mRNA. Translation: AAD25351.1. AF214657 Genomic DNA. Translation: AAF45147.1. AF214658 Genomic DNA. Translation: AAF45148.1. AB039204 Genomic DNA. Translation: BAB68728.1. AB039205 Genomic DNA. Translation: BAB68729.1. AB039206 Genomic DNA. Translation: BAB68730.1. AB039207 Genomic DNA. Translation: BAB68731.1. AB039208 Genomic DNA. Translation: BAB68732.1. AB039209 Genomic DNA. Translation: BAB68733.1. AB039210 Genomic DNA. Translation: BAB68734.1. AB039211 Genomic DNA. Translation: BAB68735.1. AB039212 Genomic DNA. Translation: BAB68736.1.
IPI	IPI00311940.
RefSeq	NP_064318.2.
UniGene	Mm.56914
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P97353.
Protein family/group databases
CAZy	GT11. Glycosyltransferase Family 11.
Proteomic databases
PRIDE	P97353.
Genome annotation databases
Ensembl	ENSMUST00000040636; ENSMUSP00000045229; ENSMUSG00000040364; Mus musculus. [Genome view]
GeneID	56546.
KEGG	mmu:56546.
Organism-specific databases
CTD	56546.
MGI	MGI:1928893. Sec1.
Phylogenomic databases
eggNOG	roNOG17828.
HOVERGEN	P97353.
InParanoid	P97353.
Enzyme and pathway databases
BRENDA	2.4.1.69. 244.
Gene expression databases
ArrayExpress	P97353.
Bgee	P97353.
CleanEx	MM_FUT10. MM_SEC1.
Genevestigator	P97353.
GermOnline	ENSMUSG00000040364. Mus musculus.
Family and domain databases
InterPro	IPR002516. Glyco_trans_11. [Graphical view]
PANTHER	PTHR11927. Glyco_trans_11. 1 hit.
Pfam	PF01531. Glyco_transf_11. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

SEC1_MOUSE

Accession

Primary (citable) accession number: P97353
Secondary accession number(s): Q91V73, Q920T3, Q920T4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	January 19, 2010
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)