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Protein

Rho-related GTP-binding protein RhoD

Gene

Rhod

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in endosome dynamics. May coordinate membrane transport with the function of the cytoskeleton. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Participates in the reorganization of actin cytoskeleton; the function seems to involve WHAMM and includes regulation of filopodia formation and actin filament bundling. Can modulate the effect of DAPK3 in reorganization of actin cytoskeleton and focal adhesion dissolution.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi24 – 318GTPBy similarity
Nucleotide bindingi71 – 755GTPBy similarity
Nucleotide bindingi129 – 1324GTPBy similarity

GO - Molecular functioni

  1. GTP binding Source: MGI
  2. protein kinase binding Source: UniProtKB

GO - Biological processi

  1. actin filament bundle assembly Source: MGI
  2. focal adhesion assembly Source: MGI
  3. lamellipodium assembly Source: MGI
  4. positive regulation of cell adhesion Source: MGI
  5. positive regulation of cell migration Source: MGI
  6. protein targeting Source: MGI
  7. regulation of actin cytoskeleton reorganization Source: UniProtKB
  8. regulation of focal adhesion assembly Source: UniProtKB
  9. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_297947. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoD
Gene namesi
Name:Rhod
Synonyms:Arhd, Rhom
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:108446. Rhod.

Subcellular locationi

Cell membrane. Early endosome 1 Publication
Note: Colocalizes with RAB5 to early endosomes.1 Publication

GO - Cellular componenti

  1. early endosome Source: UniProtKB-SubCell
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Rho-related GTP-binding protein RhoDPRO_0000198864Add
BLAST
Propeptidei208 – 2103Removed in mature formBy similarityPRO_0000223366

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei207 – 2071Cysteine methyl esterBy similarity
Lipidationi207 – 2071S-geranylgeranyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP97348.
PaxDbiP97348.
PRIDEiP97348.

PTM databases

PhosphoSiteiP97348.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiP97348.
CleanExiMM_RHOD.
ExpressionAtlasiP97348. baseline and differential.
GenevestigatoriP97348.

Interactioni

Subunit structurei

Interacts with PAK7/PAK5. Interacts (in GTP-bound form) with DAPK3, FILIP1 and WHAMM. Interacts (independent of GTP-loaded status) with ANKFY1.3 Publications

Protein-protein interaction databases

BioGridi198198. 6 interactions.
IntActiP97348. 1 interaction.
MINTiMINT-1340205.
STRINGi10090.ENSMUSP00000036031.

Structurei

3D structure databases

ProteinModelPortaliP97348.
SMRiP97348. Positions 17-193.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi46 – 549Effector regionSequence Analysis

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP97348.
KOiK07530.
OMAiAGQVDYD.
OrthoDBiEOG74TX0S.
PhylomeDBiP97348.
TreeFamiTF331746.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97348-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNASQVAGEE APQSGHSVKV VLVGDGGCGK TSLMMVFAKG AFPESYSPTV
60 70 80 90 100
FERYNATLQM KGKPVHLQIW DTAGQDDYDR LRPLFYPDAN VLLLCFDVTN
110 120 130 140 150
PNSFDNVSNR WYPEVTHFCK GVPIIVVGCK IDLRKDKVLV NNLRKKRLEP
160 170 180 190 200
VTYHRGHDMA RSVGAVAYLE CSARLHDNVE AVFQEAAEVA LSSRRHNFWR
210
RITQNCCLAT
Length:210
Mass (Da):23,562
Last modified:May 1, 1997 - v1
Checksum:i0D9C1C39AA6D56E9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84325 mRNA. Translation: CAA59064.1.
D89821 mRNA. Translation: BAA14023.1.
BC047989 mRNA. Translation: AAH47989.1.
CCDSiCCDS29427.1.
RefSeqiNP_031511.1. NM_007485.4.
UniGeneiMm.27701.

Genome annotation databases

EnsembliENSMUST00000048197; ENSMUSP00000036031; ENSMUSG00000041845.
GeneIDi11854.
KEGGimmu:11854.
UCSCiuc008gaa.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84325 mRNA. Translation: CAA59064.1.
D89821 mRNA. Translation: BAA14023.1.
BC047989 mRNA. Translation: AAH47989.1.
CCDSiCCDS29427.1.
RefSeqiNP_031511.1. NM_007485.4.
UniGeneiMm.27701.

3D structure databases

ProteinModelPortaliP97348.
SMRiP97348. Positions 17-193.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198198. 6 interactions.
IntActiP97348. 1 interaction.
MINTiMINT-1340205.
STRINGi10090.ENSMUSP00000036031.

PTM databases

PhosphoSiteiP97348.

Proteomic databases

MaxQBiP97348.
PaxDbiP97348.
PRIDEiP97348.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048197; ENSMUSP00000036031; ENSMUSG00000041845.
GeneIDi11854.
KEGGimmu:11854.
UCSCiuc008gaa.1. mouse.

Organism-specific databases

CTDi29984.
MGIiMGI:108446. Rhod.

Phylogenomic databases

eggNOGiCOG1100.
HOGENOMiHOG000233974.
HOVERGENiHBG009351.
InParanoidiP97348.
KOiK07530.
OMAiAGQVDYD.
OrthoDBiEOG74TX0S.
PhylomeDBiP97348.
TreeFamiTF331746.

Enzyme and pathway databases

ReactomeiREACT_297947. Rho GTPase cycle.

Miscellaneous databases

NextBioi279833.
PROiP97348.
SOURCEiSearch...

Gene expression databases

BgeeiP97348.
CleanExiMM_RHOD.
ExpressionAtlasiP97348. baseline and differential.
GenevestigatoriP97348.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003578. Small_GTPase_Rho.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00174. RHO. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51420. RHO. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION.
    Tissue: Kidney.
  2. "Novel small GTPase M-Ras participates in reorganization of actin cytoskeleton."
    Matsumoto K., Asano T., Endo T.
    Oncogene 15:2409-2417(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryo.
  4. "RhoD regulates cytoskeletal dynamics via the actin nucleation-promoting factor WASp homologue associated with actin Golgi membranes and microtubules."
    Gad A.K., Nehru V., Ruusala A., Aspenstrom P.
    Mol. Biol. Cell 23:4807-4819(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH FILIP1 AND WHAMM.
  5. "Interaction of RhoD and ZIP kinase modulates actin filament assembly and focal adhesion dynamics."
    Nehru V., Almeida F.N., Aspenstrom P.
    Biochem. Biophys. Res. Commun. 433:163-169(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH DAPK3.
  6. "RhoD binds the Rab5 effector Rabankyrin-5 and has a role in trafficking of the platelet-derived growth factor receptor."
    Nehru V., Voytyuk O., Lennartsson J., Aspenstroem P.
    Traffic 14:1242-1254(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ANKFY1, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiRHOD_MOUSE
AccessioniPrimary (citable) accession number: P97348
Secondary accession number(s): O09104
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: April 1, 2015
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.