ID   NKX23_MOUSE             Reviewed;         362 AA.
AC   P97334; Q9QZ60; Q9WV67;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2002, sequence version 2.
DT   24-JAN-2024, entry version 166.
DE   RecName: Full=Homeobox protein Nkx-2.3;
DE   AltName: Full=Homeobox protein NK-2 homolog 3;
DE   AltName: Full=Homeobox protein NK-2 homolog C;
DE   AltName: Full=Nkx2-C;
GN   Name=Nkx2-3; Synonyms=Nkx-2.3, Nkx2c;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=129; TISSUE=Liver;
RX   PubMed=9142493;
RX   DOI=10.1002/(sici)1097-0177(199705)209:1<29::aid-aja3>3.0.co;2-z;
RA   Pabst O., Schneider A., Brand T., Arnold H.-H.;
RT   "The mouse Nkx2-3 homeodomain gene is expressed in gut mesenchyme during
RT   pre- and postnatal mouse development.";
RL   Dev. Dyn. 209:29-35(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=129;
RA   Wang C.-C., Biben C., Robb R., Tarlinton D., Nassir F., Davidson N.O.,
RA   Harvey R.P.;
RT   "Homeodomain factor Nkx2-3 is required for normal development of the gut-
RT   associated lymphoid tissue and the spleen.";
RL   Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BALB/cJ;
RX   PubMed=10926756; DOI=10.1006/dbio.2000.9749;
RA   Wang C.-C., Biben C., Robb L., Nassir F., Barnett L., Davidson N.O.,
RA   Koentgen F., Tarlinton D., Harvey R.P.;
RT   "Homeodomain factor Nkx2-3 controls regional expression of leukocyte homing
RT   coreceptor MAdCAM-1 in specialized endothelial cells of the viscera.";
RL   Dev. Biol. 224:152-167(2000).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [5]
RP   FUNCTION.
RX   PubMed=10790368; DOI=10.1093/emboj/19.9.2015;
RA   Pabst O., Foerster R., Lipp M., Engel H., Arnold H.-H.;
RT   "NKX2.3 is required for MAdCAM-1 expression and homing of lymphocytes in
RT   spleen and mucosa-associated lymphoid tissue.";
RL   EMBO J. 19:2015-2023(2000).
RN   [6]
RP   TISSUE SPECIFICITY.
RX   PubMed=12141427;
RA   Biben C., Wang C.-C., Harvey R.P.;
RT   "NK-2 class homeobox genes and pharyngeal/oral patterning: Nkx2-3 is
RT   required for salivary gland and tooth morphogenesis.";
RL   Int. J. Dev. Biol. 46:415-422(2002).
CC   -!- FUNCTION: Transcriptional regulator essential for normal development
CC       and functions of the small intestine and spleen. Activates directly
CC       MADCAM1 expression. Required for homing of lymphocytes in spleen and
CC       mucosa-associated lymphoid tissue. May have a role during pharyngeal
CC       organogenesis. {ECO:0000269|PubMed:10790368}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in spleen and intestine. Also expressed
CC       in salivary gland and tongue. {ECO:0000269|PubMed:12141427}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in gut mesenchyme during pre- and
CC       postnatal development. Expressed as well in the pharyngeal floor and
CC       pouches, and in the oral and branchial arch ectoderm. Expression
CC       persisted in the developing thyroid until birth, in mucous forming
CC       cells of salivary glands and in odontogenic epithelium of the mandible.
CC   -!- SIMILARITY: Belongs to the NK-2 homeobox family. {ECO:0000305}.
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DR   EMBL; Y11117; CAA72002.1; -; mRNA.
DR   EMBL; AF202036; AAF08008.1; -; mRNA.
DR   EMBL; AF155583; AAD38415.1; -; Genomic_DNA.
DR   EMBL; BC072614; AAH72614.1; -; mRNA.
DR   CCDS; CCDS29833.1; -.
DR   RefSeq; NP_032725.1; NM_008699.2.
DR   AlphaFoldDB; P97334; -.
DR   SMR; P97334; -.
DR   BioGRID; 201777; 2.
DR   IntAct; P97334; 2.
DR   STRING; 10090.ENSMUSP00000050933; -.
DR   iPTMnet; P97334; -.
DR   PhosphoSitePlus; P97334; -.
DR   PaxDb; 10090-ENSMUSP00000050933; -.
DR   ProteomicsDB; 293571; -.
DR   Antibodypedia; 31083; 100 antibodies from 25 providers.
DR   DNASU; 18089; -.
DR   Ensembl; ENSMUST00000057178.11; ENSMUSP00000050933.10; ENSMUSG00000044220.14.
DR   GeneID; 18089; -.
DR   KEGG; mmu:18089; -.
DR   UCSC; uc008hok.1; mouse.
DR   AGR; MGI:97348; -.
DR   CTD; 159296; -.
DR   MGI; MGI:97348; Nkx2-3.
DR   VEuPathDB; HostDB:ENSMUSG00000044220; -.
DR   eggNOG; KOG0842; Eukaryota.
DR   GeneTree; ENSGT00940000157556; -.
DR   HOGENOM; CLU_049543_0_0_1; -.
DR   InParanoid; P97334; -.
DR   OMA; CVTPGAQ; -.
DR   OrthoDB; 461623at2759; -.
DR   PhylomeDB; P97334; -.
DR   TreeFam; TF351204; -.
DR   BioGRID-ORCS; 18089; 1 hit in 79 CRISPR screens.
DR   ChiTaRS; Nkx2-3; mouse.
DR   PRO; PR:P97334; -.
DR   Proteomes; UP000000589; Chromosome 19.
DR   RNAct; P97334; Protein.
DR   Bgee; ENSMUSG00000044220; Expressed in hindgut and 49 other cell types or tissues.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0030183; P:B cell differentiation; IMP:MGI.
DR   GO; GO:0043367; P:CD4-positive, alpha-beta T cell differentiation; IMP:MGI.
DR   GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR   GO; GO:0048565; P:digestive tract development; IMP:MGI.
DR   GO; GO:0022612; P:gland morphogenesis; IMP:MGI.
DR   GO; GO:0006955; P:immune response; IMP:MGI.
DR   GO; GO:0001776; P:leukocyte homeostasis; IMP:MGI.
DR   GO; GO:0050900; P:leukocyte migration; IMP:MGI.
DR   GO; GO:0048535; P:lymph node development; IMP:MGI.
DR   GO; GO:0030225; P:macrophage differentiation; IMP:MGI.
DR   GO; GO:0048537; P:mucosa-associated lymphoid tissue development; IMP:MGI.
DR   GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IMP:MGI.
DR   GO; GO:0048541; P:Peyer's patch development; IMP:MGI.
DR   GO; GO:0002317; P:plasma cell differentiation; IMP:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR   GO; GO:0009791; P:post-embryonic development; IMP:MGI.
DR   GO; GO:0048621; P:post-embryonic digestive tract morphogenesis; IMP:MGI.
DR   GO; GO:0050678; P:regulation of epithelial cell proliferation; IMP:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0048536; P:spleen development; IMP:MGI.
DR   GO; GO:0006366; P:transcription by RNA polymerase II; IMP:MGI.
DR   GO; GO:0006641; P:triglyceride metabolic process; IMP:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   PANTHER; PTHR24340; HOMEOBOX PROTEIN NKX; 1.
DR   PANTHER; PTHR24340:SF32; HOMEOBOX PROTEIN NKX-2.3; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; P97334; MM.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..362
FT                   /note="Homeobox protein Nkx-2.3"
FT                   /id="PRO_0000048934"
FT   DNA_BIND        145..204
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          126..149
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          203..222
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        128..143
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        44
FT                   /note="C -> R (in Ref. 1; CAA72002)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        61
FT                   /note="D -> E (in Ref. 1; CAA72002)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        132
FT                   /note="K -> R (in Ref. 1; CAA72002)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        248..250
FT                   /note="GVG -> RC (in Ref. 1; CAA72002)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        261..357
FT                   /note="AYGYGNSAAAAAAAAAAAAAAAAYSGSYGCAYPTGGGGGGGGTASAATTAMQ
FT                   PACSATGGGSFVNVSNLGGFGSGGGAQPLHQGAAAGSACTQGTLQ -> TATGTRPPQP
FT                   LQPPQPQQQGLQRQLRLAYRPVAAVVVAARPPRRPPPCNPPAAPPGDLRVTAGIEAFVP
FT                   LVR (in Ref. 1; CAA72002)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        349
FT                   /note="S -> A (in Ref. 3; AAD38415)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   362 AA;  38090 MW;  48886528EC3E1622 CRC64;
     MMLPSPVTST PFSVKDILNL EQQRHFHGAH LQAELEQHFH SAPCMLATAE GTQFSDAGEE
     DEEEEGEKLS YLNSLAAAEG HGDSGLCPQS YVHTVLRDAC SGPKEQEEEV VSERSQKSCQ
     LKKSLEAAGD CKTSEDGERP KPRSRRKPRV LFSQAQVFEL ERRFKQQRYL SAPEREHLAS
     SLKLTSTQVK IWFQNRRYKC KRQRQDKSLE LGTHAPPPPP RRVAVPVLVR DGKPCVTPSA
     QTYGSPYGVG AGAYSYNSFP AYGYGNSAAA AAAAAAAAAA AAAYSGSYGC AYPTGGGGGG
     GGTASAATTA MQPACSATGG GSFVNVSNLG GFGSGGGAQP LHQGAAAGSA CTQGTLQGIR
     AW
//