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P97334 (NKX23_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Homeobox protein Nkx-2.3
Alternative name(s):
Homeobox protein NK-2 homolog 3
Homeobox protein NK-2 homolog C
Nkx2-C
Gene names
Name:Nkx2-3
Synonyms:Nkx-2.3, Nkx2c
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length362 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional regulator essential for normal development and functions of the small intestine and spleen. Activates directly MADCAM1 expression. Required for homing of lymphocytes in spleen and mucosa-associated lymphoid tissue. May have a role during pharyngeal organogenesis. Ref.5

Subcellular location

Nucleus Probable.

Tissue specificity

Expressed in spleen and intestine. Also expressed in salivary gland and tongue. Ref.6

Developmental stage

Expressed in gut mesenchyme during pre- and postnatal development. Expressed as well in the pharyngeal floor and pouches, and in the oral and branchial arch ectoderm. Expression persisted in the developing thyroid until birth, in mucous forming cells of salivary glands and in odontogenic epithelium of the mandible.

Sequence similarities

Belongs to the NK-2 homeobox family.

Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell differentiation

Inferred from mutant phenotype PubMed 12682228. Source: MGI

CD4-positive, alpha-beta T cell differentiation

Inferred from mutant phenotype Ref.3. Source: MGI

Peyer's patch development

Inferred from mutant phenotype Ref.5. Source: MGI

digestive tract development

Inferred from mutant phenotype PubMed 10207146Ref.3. Source: MGI

gland morphogenesis

Inferred from mutant phenotype Ref.3. Source: MGI

immune response

Inferred from mutant phenotype PubMed 12682228. Source: MGI

leukocyte homeostasis

Inferred from mutant phenotype Ref.3. Source: MGI

leukocyte migration

Inferred from mutant phenotype Ref.5Ref.3. Source: MGI

lymph node development

Inferred from mutant phenotype Ref.5Ref.3. Source: MGI

macrophage differentiation

Inferred from mutant phenotype Ref.3. Source: MGI

mucosal-associated lymphoid tissue development

Inferred from mutant phenotype Ref.3. Source: MGI

odontogenesis of dentin-containing tooth

Inferred from mutant phenotype Ref.6. Source: MGI

plasma cell differentiation

Inferred from mutant phenotype Ref.3. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay Ref.5. Source: MGI

post-embryonic development

Inferred from mutant phenotype Ref.3. Source: MGI

post-embryonic digestive tract morphogenesis

Inferred from mutant phenotype Ref.3. Source: MGI

regulation of cell proliferation

Inferred from mutant phenotype PubMed 10207146. Source: MGI

spleen development

Inferred from mutant phenotype PubMed 10207146Ref.5Ref.3. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

triglyceride metabolic process

Inferred from mutant phenotype Ref.3. Source: MGI

   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsequence-specific DNA binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 362362Homeobox protein Nkx-2.3
PRO_0000048934

Regions

DNA binding145 – 20460Homeobox
Compositional bias59 – 679Poly-Glu
Compositional bias216 – 2205Poly-Pro
Compositional bias268 – 28316Poly-Ala
Compositional bias295 – 3028Poly-Gly

Experimental info

Sequence conflict441C → R in CAA72002. Ref.1
Sequence conflict611D → E in CAA72002. Ref.1
Sequence conflict1321K → R in CAA72002. Ref.1
Sequence conflict248 – 2503GVG → RC in CAA72002. Ref.1
Sequence conflict261 – 35797AYGYG…QGTLQ → TATGTRPPQPLQPPQPQQQG LQRQLRLAYRPVAAVVVAAR PPRRPPPCNPPAAPPGDLRV TAGIEAFVPLVR in CAA72002. Ref.1
Sequence conflict3491S → A in AAD38415. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P97334 [UniParc].

Last modified August 2, 2002. Version 2.
Checksum: 48886528EC3E1622

FASTA36238,090
        10         20         30         40         50         60 
MMLPSPVTST PFSVKDILNL EQQRHFHGAH LQAELEQHFH SAPCMLATAE GTQFSDAGEE 

        70         80         90        100        110        120 
DEEEEGEKLS YLNSLAAAEG HGDSGLCPQS YVHTVLRDAC SGPKEQEEEV VSERSQKSCQ 

       130        140        150        160        170        180 
LKKSLEAAGD CKTSEDGERP KPRSRRKPRV LFSQAQVFEL ERRFKQQRYL SAPEREHLAS 

       190        200        210        220        230        240 
SLKLTSTQVK IWFQNRRYKC KRQRQDKSLE LGTHAPPPPP RRVAVPVLVR DGKPCVTPSA 

       250        260        270        280        290        300 
QTYGSPYGVG AGAYSYNSFP AYGYGNSAAA AAAAAAAAAA AAAYSGSYGC AYPTGGGGGG 

       310        320        330        340        350        360 
GGTASAATTA MQPACSATGG GSFVNVSNLG GFGSGGGAQP LHQGAAAGSA CTQGTLQGIR 


AW 

« Hide

References

« Hide 'large scale' references
[1]"The mouse Nkx2-3 homeodomain gene is expressed in gut mesenchyme during pre- and postnatal mouse development."
Pabst O., Schneider A., Brand T., Arnold H.-H.
Dev. Dyn. 209:29-35(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: 129.
Tissue: Liver.
[2]"Homeodomain factor Nkx2-3 is required for normal development of the gut-associated lymphoid tissue and the spleen."
Wang C.-C., Biben C., Robb R., Tarlinton D., Nassir F., Davidson N.O., Harvey R.P.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: 129.
[3]"Homeodomain factor Nkx2-3 controls regional expression of leukocyte homing coreceptor MAdCAM-1 in specialized endothelial cells of the viscera."
Wang C.-C., Biben C., Robb L., Nassir F., Barnett L., Davidson N.O., Koentgen F., Tarlinton D., Harvey R.P.
Dev. Biol. 224:152-167(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: BALB/c.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[5]"NKX2.3 is required for MAdCAM-1 expression and homing of lymphocytes in spleen and mucosa-associated lymphoid tissue."
Pabst O., Foerster R., Lipp M., Engel H., Arnold H.-H.
EMBO J. 19:2015-2023(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"NK-2 class homeobox genes and pharyngeal/oral patterning: Nkx2-3 is required for salivary gland and tooth morphogenesis."
Biben C., Wang C.-C., Harvey R.P.
Int. J. Dev. Biol. 46:415-422(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y11117 mRNA. Translation: CAA72002.1.
AF202036 mRNA. Translation: AAF08008.1.
AF155583 Genomic DNA. Translation: AAD38415.1.
BC072614 mRNA. Translation: AAH72614.1.
CCDSCCDS29833.1.
RefSeqNP_032725.1. NM_008699.2.
XP_006526807.1. XM_006526744.1.
UniGeneMm.56994.

3D structure databases

ProteinModelPortalP97334.
SMRP97334. Positions 145-199.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201777. 2 interactions.

PTM databases

PhosphoSiteP97334.

Proteomic databases

PRIDEP97334.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000057178; ENSMUSP00000050933; ENSMUSG00000044220.
GeneID18089.
KEGGmmu:18089.
UCSCuc008hok.1. mouse.

Organism-specific databases

CTD159296.
MGIMGI:97348. Nkx2-3.

Phylogenomic databases

eggNOGNOG310976.
GeneTreeENSGT00720000108380.
HOGENOMHOG000231923.
HOVERGENHBG006689.
InParanoidP97334.
KOK09343.
OMAPCVTPSA.
OrthoDBEOG769ZK0.
TreeFamTF351204.

Gene expression databases

BgeeP97334.
GenevestigatorP97334.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSPR00024. HOMEOBOX.
SMARTSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNKX2-3. mouse.
NextBio293231.
PROP97334.
SOURCESearch...

Entry information

Entry nameNKX23_MOUSE
AccessionPrimary (citable) accession number: P97334
Secondary accession number(s): Q9QZ60, Q9WV67
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: August 2, 2002
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot