P97334 (NKX23_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homeobox protein Nkx-2.3

Alternative name(s):
Homeobox protein NK-2 homolog 3
Homeobox protein NK-2 homolog C
Nkx2-C

Gene names

Name:	Nkx2-3
Synonyms:	Nkx-2.3, Nkx2c

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	362 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Transcriptional regulator essential for normal development and functions of the small intestine and spleen. Activates directly MADCAM1 expression. Required for homing of lymphocytes in spleen and mucosa-associated lymphoid tissue. May have a role during pharyngeal organogenesis. Ref.5
Subcellular location	Nucleus Probable.
Tissue specificity	Expressed in spleen and intestine. Also expressed in salivary gland and tongue. Ref.6
Developmental stage	Expressed in gut mesenchyme during pre- and postnatal development. Expressed as well in the pharyngeal floor and pouches, and in the oral and branchial arch ectoderm. Expression persisted in the developing thyroid until birth, in mucous forming cells of salivary glands and in odontogenic epithelium of the mandible.
Sequence similarities	Belongs to the NK-2 homeobox family. Contains 1 homeobox DNA-binding domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Homeobox
Ligand	DNA-binding
Molecular function	Developmental protein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CD4-positive, alpha-beta T cell differentiation Inferred from mutant phenotype Ref.3. Source: MGI Peyer's patch development Inferred from mutant phenotype Ref.5. Source: MGI gland morphogenesis Inferred from mutant phenotype Ref.3. Source: MGI leukocyte homeostasis Inferred from mutant phenotype Ref.3. Source: MGI leukocyte migration Inferred from mutant phenotype Ref.5Ref.3. Source: MGI lymph node development Inferred from mutant phenotype Ref.5Ref.3. Source: MGI macrophage differentiation Inferred from mutant phenotype Ref.3. Source: MGI odontogenesis of dentin-containing tooth Inferred from mutant phenotype Ref.6. Source: MGI plasma cell differentiation Inferred from mutant phenotype Ref.3. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay Ref.5. Source: MGI post-embryonic digestive tract morphogenesis Inferred from mutant phenotype Ref.3. Source: MGI regulation of cell proliferation Inferred from mutant phenotype PubMed 10207146. Source: MGI spleen development Inferred from mutant phenotype PubMed 10207146Ref.5Ref.3. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW triglyceride metabolic process Inferred from mutant phenotype Ref.3. Source: MGI
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 362	362	Homeobox protein Nkx-2.3		PRO_0000048934
Regions
	DNA binding	145 – 204	60	Homeobox
	Compositional bias	59 – 67	9	Poly-Glu
	Compositional bias	216 – 220	5	Poly-Pro
	Compositional bias	268 – 283	16	Poly-Ala
	Compositional bias	295 – 302	8	Poly-Gly
Experimental info
	Sequence conflict	44	1	C → R in CAA72002. Ref.1
	Sequence conflict	61	1	D → E in CAA72002. Ref.1
	Sequence conflict	132	1	K → R in CAA72002. Ref.1
	Sequence conflict	248 – 250	3	GVG → RC in CAA72002. Ref.1
	Sequence conflict	261 – 357	97	AYGYG…QGTLQ → TATGTRPPQPLQPPQPQQQG LQRQLRLAYRPVAAVVVAAR PPRRPPPCNPPAAPPGDLRV TAGIEAFVPLVR in CAA72002. Ref.1
	Sequence conflict	349	1	S → A in AAD38415. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P97334 [UniParc]. Last modified August 2, 2002. Version 2. Checksum: 48886528EC3E1622	FASTA	362	38,090
	10 20 30 40 50 60 MMLPSPVTST PFSVKDILNL EQQRHFHGAH LQAELEQHFH SAPCMLATAE GTQFSDAGEE 70 80 90 100 110 120 DEEEEGEKLS YLNSLAAAEG HGDSGLCPQS YVHTVLRDAC SGPKEQEEEV VSERSQKSCQ 130 140 150 160 170 180 LKKSLEAAGD CKTSEDGERP KPRSRRKPRV LFSQAQVFEL ERRFKQQRYL SAPEREHLAS 190 200 210 220 230 240 SLKLTSTQVK IWFQNRRYKC KRQRQDKSLE LGTHAPPPPP RRVAVPVLVR DGKPCVTPSA 250 260 270 280 290 300 QTYGSPYGVG AGAYSYNSFP AYGYGNSAAA AAAAAAAAAA AAAYSGSYGC AYPTGGGGGG 310 320 330 340 350 360 GGTASAATTA MQPACSATGG GSFVNVSNLG GFGSGGGAQP LHQGAAAGSA CTQGTLQGIR AW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The mouse Nkx2-3 homeodomain gene is expressed in gut mesenchyme during pre- and postnatal mouse development." Pabst O., Schneider A., Brand T., Arnold H.-H. Dev. Dyn. 209:29-35(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: 129. Tissue: Liver.
[2]	"Homeodomain factor Nkx2-3 is required for normal development of the gut-associated lymphoid tissue and the spleen." Wang C.-C., Biben C., Robb R., Tarlinton D., Nassir F., Davidson N.O., Harvey R.P. Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: 129.
[3]	"Homeodomain factor Nkx2-3 controls regional expression of leukocyte homing coreceptor MAdCAM-1 in specialized endothelial cells of the viscera." Wang C.-C., Biben C., Robb L., Nassir F., Barnett L., Davidson N.O., Koentgen F., Tarlinton D., Harvey R.P. Dev. Biol. 224:152-167(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c.
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[5]	"NKX2.3 is required for MAdCAM-1 expression and homing of lymphocytes in spleen and mucosa-associated lymphoid tissue." Pabst O., Foerster R., Lipp M., Engel H., Arnold H.-H. EMBO J. 19:2015-2023(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"NK-2 class homeobox genes and pharyngeal/oral patterning: Nkx2-3 is required for salivary gland and tooth morphogenesis." Biben C., Wang C.-C., Harvey R.P. Int. J. Dev. Biol. 46:415-422(2002) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y11117 mRNA. Translation: CAA72002.1. AF202036 mRNA. Translation: AAF08008.1. AF155583 Genomic DNA. Translation: AAD38415.1. BC072614 mRNA. Translation: AAH72614.1.
IPI	IPI00124004.
RefSeq	NP_032725.1. NM_008699.2.
UniGene	Mm.56994.
3D structure databases
ProteinModelPortal	P97334.
SMR	P97334. Positions 145-199.
ModBase	Search...
PTM databases
PhosphoSite	P97334.
Proteomic databases
PRIDE	P97334.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000057178; ENSMUSP00000050933; ENSMUSG00000044220.
GeneID	18089.
KEGG	mmu:18089.
UCSC	uc008hok.1. mouse.
Organism-specific databases
CTD	159296.
MGI	MGI:97348. Nkx2-3.
Phylogenomic databases
eggNOG	NOG310976.
GeneTree	ENSGT00700000104098.
HOGENOM	HOG000231923.
HOVERGEN	HBG006689.
InParanoid	P97334.
KO	K09343.
OMA	YGCAYPA.
OrthoDB	EOG4WM4WF.
Gene expression databases
Bgee	P97334.
Genevestigator	P97334.
GermOnline	ENSMUSG00000044220. Mus musculus.
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR017970. Homeobox_CS. IPR020479. Homeobox_metazoa. IPR001356. Homeodomain. IPR009057. Homeodomain-like. [Graphical view]
Pfam	PF00046. Homeobox. 1 hit. [Graphical view]
PRINTS	PR00024. HOMEOBOX.
SMART	SM00389. HOX. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
PROSITE	PS00027. HOMEOBOX_1. 1 hit. PS50071. HOMEOBOX_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	NKX2-3. mouse.
NextBio	293231.
SOURCE	Search...

Entry information

Entry name

NKX23_MOUSE

Accession

Primary (citable) accession number: P97334
Secondary accession number(s): Q9QZ60, Q9WV67

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	August 2, 2002
Last modified:	April 3, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program