Reviewed, UniProtKB/Swiss-Prot P97333 (NRP1_MOUSE)

Names and origin

Protein names

Recommended name:
    Neuropilin-1
Alternative name(s):
    A5 protein
    CD_antigen=CD304

Gene names

Name:	Nrp1
Synonyms:	Nrp

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis By similarity.
Subunit structure	Heterodimer with NRP2 Probable. Binds PLXNB1 By similarity.
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Nervous system.
Sequence similarities	Belongs to the neuropilin family. Contains 2 CUB domains. Contains 2 F5/8 type C domains. Contains 1 MAM domain.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation Neurogenesis
Cellular component	Membrane
Domain	Repeat Signal Transmembrane
Molecular function	Developmental protein Receptor
PTM	Disulfide bond Glycoprotein Heparan sulfate Phosphoprotein Proteoglycan
Gene Ontology (GO)
Biological process	axon guidance Inferred from mutant phenotype. Source: MGI axonal fasciculation Inferred from mutant phenotype. Source: MGI cell adhesion Inferred from electronic annotation. Source: InterPro cell migration Inferred from mutant phenotype. Source: MGI dendrite development Inferred from mutant phenotype. Source: MGI heart development Inferred from genetic interaction. Source: MGI negative regulation of axon extension involved in axon guidance Inferred from direct assay. Source: MGI patterning of blood vessels Inferred from mutant phenotype. Source: MGI vascular endothelial growth factor receptor signaling pathway Inferred from mutant phenotype. Source: MGI
Cellular component	axon Inferred from direct assay. Source: MGI integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred by curator. Source: MGI
Molecular function	identical protein binding Inferred from physical interaction. Source: IntAct semaphorin receptor activity Inferred from genetic interaction. Source: MGI
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-1555129,EBI-1555129

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 923	902	Neuropilin-1		PRO_0000021860
Regions
	Topological domain	22 – 856	835	Extracellular Potential
	Transmembrane	857 – 879	23	Potential
	Topological domain	880 – 923	44	Cytoplasmic Potential
	Domain	27 – 141	115	CUB 1
	Domain	147 – 265	119	CUB 2
	Domain	275 – 424	150	F5/8 type C 1
	Domain	431 – 583	153	F5/8 type C 2
	Domain	645 – 811	167	MAM
Amino acid modifications
	Modified residue	920	1	Phosphotyrosine By similarity
	Glycosylation	150	1	N-linked (GlcNAc...) Potential
	Glycosylation	261	1	N-linked (GlcNAc...) Potential
	Glycosylation	300	1	N-linked (GlcNAc...) Potential
	Glycosylation	522	1	N-linked (GlcNAc...) Potential
	Glycosylation	612	1	O-linked (Xyl...) (chondroitin sulfate); alternate By similarity
	Glycosylation	612	1	O-linked (Xyl...) (heparan sulfate); alternate By similarity
	Glycosylation	842	1	N-linked (GlcNAc...) Potential
	Disulfide bond	27 ↔ 54		Probable
	Disulfide bond	82 ↔ 104		Probable
	Disulfide bond	147 ↔ 173		Probable
	Disulfide bond	206 ↔ 228		Probable
	Disulfide bond	275 ↔ 424		By similarity
	Disulfide bond	431 ↔ 583		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97333-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 0644B8A170796808	FASTA	923	103,020
	10 20 30 40 50 60 MERGLPLLCA TLALALALAG AFRSDKCGGT IKIENPGYLT SPGYPHSYHP SEKCEWLIQA 70 80 90 100 110 120 PEPYQRIIIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW GKFCGKIAPS PVVSSGPFLF 130 140 150 160 170 180 IKFVSDYETH GAGFSIRYEI FKRGPECSQN YTAPTGVIKS PGFPEKYPNC LECTYIIFAP 190 200 210 220 230 240 KMSEIILEFE SFDLEQDSNP PGGMFCRYDR LEIWDGFPEV GPHIGRYCGQ KTPGRIRSSS 250 260 270 280 290 300 GVLSMVFYTD SAIAKEGFSA NYSVLQSSIS EDFKCMEALG MESGEIHSDQ ITASSQYGTN 310 320 330 340 350 360 WSVERSRLNY PENGWTPGED SYKEWIQVDL GLLRFVTAVG TQGAISKETK KKYYVKTYRV 370 380 390 400 410 420 DISSNGEDWI SLKEGNKAII FQGNTNPTDV VLGVFSKPLI TRFVRIKPVS WETGISMRFE 430 440 450 460 470 480 VYGCKITDYP CSGMLGMVSG LISDSQITAS NQADRNWMPE NIRLVTSRTG WALPPSPHPY 490 500 510 520 530 540 TNEWLQVDLG DEKIVRGVII QGGKHRENKV FMRKFKIAYS NNGSDWKTIM DDSKRKAKSF 550 560 570 580 590 600 EGNNNYDTPE LRTFSPLSTR FIRIYPERAT HSGLGLRMEL LGCEVEAPTA GPTTPNGNPV 610 620 630 640 650 660 HECDDDQANC HSGTGDDFQL TGGTTVLATE KPTIIDSTIQ SEFPTYGFNC EFGWGSHKTF 670 680 690 700 710 720 CHWEHDSHAQ LRWSVLTSKT GPIQDHTGDG NFIYSQADEN QKGKVARLVS PVVYSQSSAH 730 740 750 760 770 780 CMTFWYHMSG SHVGTLRVKL RYQKPEEYDQ LVWMVVGHQG DHWKEGRVLL HKSLKLYQVI 790 800 810 820 830 840 FEGEIGKGNL GGIAVDDISI NNHISQEDCA KPTDLDKKNT EIKIDETGST PGYEGEGEGD 850 860 870 880 890 900 KNISRKPGNV LKTLDPILIT IIAMSALGVL LGAVCGVVLY CACWHNGMSE RNLSALENYN 910 920 FELVDGVKLK KDKLNPQSNY SEA

References

[1]	"Developmentally regulated expression of a cell surface protein, neuropilin, in the mouse nervous system." Kawakami A., Kitsukawa T., Takagi S., Fujisawa H. J. Neurobiol. 29:1-17(1996) [PubMed: 8748368] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Embryonic brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50086 mRNA. Translation: BAA08789.1.
IPI	IPI00123996.
RefSeq	NP_032763.1.
UniGene	Mm.271745 Mm.423531
3D structure databases
HSSP	HSSP built from PDB template 1KEX based on UniProtKB O14786.
SMR	P97333. Positions 274-586.
ModBase	Search...
Protein-protein interaction databases
IntAct	P97333. 1 interaction.
PTM databases
PhosphoSite	P97333.
Proteomic databases
PRIDE	P97333.
Genome annotation databases
Ensembl	ENSMUSG00000025810. Mus musculus. [Contig view]
GeneID	18186.
KEGG	mmu:18186.
Organism-specific databases
MGI	MGI:106206. Nrp1.
Phylogenomic databases
HOGENOM	P97333.
HOVERGEN	P97333.
Gene expression databases
ArrayExpress	P97333.
Bgee	P97333.
CleanEx	MM_NRP1.
GermOnline	ENSMUSG00000025810. Mus musculus.
Family and domain databases
InterPro	IPR000421. Coagulation_factor_5/8-type_C. IPR000859. CUB. IPR000998. MAM. IPR014648. Neuropilin. [Graphical view]
Gene3D	G3DSA:2.60.120.290. CUB. 2 hits.
Pfam	PF00431. CUB. 2 hits. PF00754. F5_F8_type_C. 2 hits. PF00629. MAM. 1 hit. [Graphical view]
PIRSF	PIRSF036960. Neuropilin. 1 hit.
PRINTS	PR00020. MAMDOMAIN.
SMART	SM00042. CUB. 2 hits. SM00231. FA58C. 2 hits. SM00137. MAM. 1 hit. [Graphical view]
PROSITE	PS01180. CUB. 2 hits. PS01285. FA58C_1. 2 hits. PS01286. FA58C_2. 2 hits. PS50022. FA58C_3. 2 hits. PS00740. MAM_1. 1 hit. PS50060. MAM_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	293506.
SOURCE	Search...

Entry information

Entry name

NRP1_MOUSE

Accession

Primary (citable) accession number: P97333

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)