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P97333 (NRP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuropilin-1
Alternative name(s):
A5 protein
CD_antigen=CD304
Gene names
Name:Nrp1
Synonyms:Nrp
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length923 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, the PLGF-2 isoform ofPGF, the VEGF-165 isoform ofVEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis By similarity.

Subunit structure

Homodimer, and heterodimer with NRP2. Binds PLXNB1 By similarity. Interacts with FER. Ref.5

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Nervous system.

Domain

The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding By similarity.

Sequence similarities

Belongs to the neuropilin family.

Contains 2 CUB domains.

Contains 2 F5/8 type C domains.

Contains 1 MAM domain.

Ontologies

Keywords
   Biological processAngiogenesis
Differentiation
Neurogenesis
   Cellular componentMembrane
   DomainRepeat
Signal
Transmembrane
Transmembrane helix
   LigandCalcium
Heparin-binding
Metal-binding
   Molecular functionDevelopmental protein
Receptor
   PTMDisulfide bond
Glycoprotein
Heparan sulfate
Proteoglycan
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processVEGF-activated neuropilin signaling pathway involved in axon guidance

Inferred from mutant phenotype PubMed 21658587. Source: BHF-UCL

angiogenesis

Inferred from mutant phenotype. Source: MGI

axon extension involved in axon guidance

Inferred from mutant phenotype PubMed 22790009. Source: BHF-UCL

axon guidance

Inferred from direct assay PubMed 15814794. Source: MGI

axonal fasciculation

Inferred from mutant phenotype PubMed 17428830. Source: MGI

axonogenesis involved in innervation

Inferred from mutant phenotype PubMed 22790009. Source: BHF-UCL

branchiomotor neuron axon guidance

Inferred from mutant phenotype PubMed 18804103. Source: ParkinsonsUK-UCL

cell adhesion

Inferred from electronic annotation. Source: InterPro

cell migration

Inferred from mutant phenotype PubMed 15376331. Source: MGI

cell migration involved in sprouting angiogenesis

Inferred from mutant phenotype PubMed 23315162. Source: BHF-UCL

cellular response to hepatocyte growth factor stimulus

Inferred from electronic annotation. Source: Ensembl

commissural neuron axon guidance

Inferred from mutant phenotype PubMed 21658587. Source: BHF-UCL

dendrite development

Inferred from mutant phenotype PubMed 12852851. Source: MGI

dichotomous subdivision of terminal units involved in salivary gland branching

Inferred from mutant phenotype PubMed 17626059. Source: MGI

endothelial cell chemotaxis

Inferred from electronic annotation. Source: Ensembl

facial nerve structural organization

Inferred from mutant phenotype PubMed 18804103. Source: ParkinsonsUK-UCL

gonadotrophin-releasing hormone neuronal migration to the hypothalamus

Inferred from mutant phenotype PubMed 21059704. Source: BHF-UCL

heart development

Inferred from genetic interaction PubMed 12852851. Source: MGI

hepatocyte growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

motor neuron axon guidance

Inferred from mutant phenotype PubMed 18804103. Source: ParkinsonsUK-UCL

negative regulation of axon extension

Inferred from genetic interaction PubMed 12852851. Source: MGI

negative regulation of axon extension involved in axon guidance

Inferred from direct assay PubMed 9753685. Source: MGI

negative regulation of extrinsic apoptotic signaling pathway

Inferred from mutant phenotype PubMed 21828096. Source: BHF-UCL

negative regulation of neuron apoptotic process

Inferred from mutant phenotype PubMed 21828096. Source: BHF-UCL

nerve development

Inferred from mutant phenotype PubMed 22790009. Source: BHF-UCL

neural crest cell migration involved in autonomic nervous system development

Inferred from mutant phenotype PubMed 19325129. Source: BHF-UCL

neuron development

Inferred from mutant phenotype PubMed 21828096. Source: BHF-UCL

neuron migration

Inferred from mutant phenotype PubMed 22790009. Source: BHF-UCL

patterning of blood vessels

Inferred from mutant phenotype PubMed 21852397PubMed 23315162PubMed 24401374. Source: BHF-UCL

platelet-derived growth factor receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

positive chemotaxis

Inferred from mutant phenotype PubMed 21658587. Source: BHF-UCL

positive regulation of axon extension involved in axon guidance

Inferred from mutant phenotype PubMed 21658587. Source: BHF-UCL

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of retinal ganglion cell axon guidance

Inferred from mutant phenotype PubMed 21658587. Source: BHF-UCL

regulation of axon extension involved in axon guidance

Inferred from direct assay PubMed 15814794. Source: MGI

regulation of retinal ganglion cell axon guidance

Inferred from mutant phenotype PubMed 21658587. Source: BHF-UCL

response to wounding

Inferred from electronic annotation. Source: Ensembl

retinal ganglion cell axon guidance

Inferred from mutant phenotype PubMed 21658587. Source: BHF-UCL

semaphorin-plexin signaling pathway

Inferred from direct assay PubMed 15239958. Source: MGI

semaphorin-plexin signaling pathway involved in axon guidance

Inferred from mutant phenotype PubMed 18804103. Source: ParkinsonsUK-UCL

semaphorin-plexin signaling pathway involved in neuron projection guidance

Inferred from mutant phenotype PubMed 22790009. Source: BHF-UCL

sympathetic ganglion development

Inferred from mutant phenotype PubMed 19325129PubMed 22790009. Source: BHF-UCL

sympathetic nervous system development

Inferred from mutant phenotype PubMed 15814794. Source: MGI

sympathetic neuron projection extension

Inferred from mutant phenotype PubMed 22790009. Source: BHF-UCL

sympathetic neuron projection guidance

Inferred from mutant phenotype PubMed 22790009. Source: BHF-UCL

trigeminal nerve morphogenesis

Inferred from mutant phenotype PubMed 15814794. Source: MGI

trigeminal nerve structural organization

Inferred from mutant phenotype PubMed 18804103. Source: ParkinsonsUK-UCL

vascular endothelial growth factor receptor signaling pathway

Inferred from mutant phenotype. Source: MGI

   Cellular_componentaxon

Inferred from direct assay PubMed 21658587PubMed 21828096. Source: BHF-UCL

cell surface

Inferred from electronic annotation. Source: Ensembl

cytosol

Inferred from electronic annotation. Source: Ensembl

growth cone

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

neurofilament

Inferred from direct assay PubMed 15814794. Source: MGI

neuronal cell body

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functiongrowth factor binding

Inferred from physical interaction PubMed 10705382. Source: MGI

heparin binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 18464795. Source: IntAct

semaphorin receptor activity

Inferred from direct assay PubMed 15239958. Source: MGI

vascular endothelial growth factor-activated receptor activity

Inferred from mutant phenotype PubMed 21658587. Source: BHF-UCL

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ptk2P341522EBI-1555129,EBI-77070

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2121 Potential
Chain22 – 923902Neuropilin-1
PRO_0000021860

Regions

Topological domain22 – 856835Extracellular Potential
Transmembrane857 – 87923Helical; Potential
Topological domain880 – 92344Cytoplasmic Potential
Domain27 – 141115CUB 1
Domain147 – 265119CUB 2
Domain275 – 424150F5/8 type C 1
Domain431 – 583153F5/8 type C 2
Domain645 – 811167MAM

Sites

Metal binding1951Calcium By similarity
Metal binding2091Calcium By similarity
Metal binding2501Calcium By similarity

Amino acid modifications

Glycosylation1501N-linked (GlcNAc...) Potential
Glycosylation2611N-linked (GlcNAc...) Ref.4
Glycosylation3001N-linked (GlcNAc...) Potential
Glycosylation5221N-linked (GlcNAc...) Ref.4
Glycosylation6121O-linked (Xyl...) (chondroitin sulfate); alternate By similarity
Glycosylation6121O-linked (Xyl...) (heparan sulfate); alternate By similarity
Glycosylation8421N-linked (GlcNAc...) Potential
Disulfide bond27 ↔ 54 Probable
Disulfide bond82 ↔ 104 Probable
Disulfide bond147 ↔ 173 By similarity
Disulfide bond206 ↔ 228 By similarity
Disulfide bond275 ↔ 424 By similarity
Disulfide bond431 ↔ 583 By similarity

Experimental info

Sequence conflict681M → I in BAA08789. Ref.1
Sequence conflict1701S → C in BAA08789. Ref.1
Sequence conflict6011D → H in BAA08789. Ref.1

Secondary structure

...................................................................................................... 923
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P97333 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 23209818C5C42137

FASTA923103,000
        10         20         30         40         50         60 
MERGLPLLCA TLALALALAG AFRSDKCGGT IKIENPGYLT SPGYPHSYHP SEKCEWLIQA 

        70         80         90        100        110        120 
PEPYQRIMIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW GKFCGKIAPS PVVSSGPFLF 

       130        140        150        160        170        180 
IKFVSDYETH GAGFSIRYEI FKRGPECSQN YTAPTGVIKS PGFPEKYPNS LECTYIIFAP 

       190        200        210        220        230        240 
KMSEIILEFE SFDLEQDSNP PGGMFCRYDR LEIWDGFPEV GPHIGRYCGQ KTPGRIRSSS 

       250        260        270        280        290        300 
GVLSMVFYTD SAIAKEGFSA NYSVLQSSIS EDFKCMEALG MESGEIHSDQ ITASSQYGTN 

       310        320        330        340        350        360 
WSVERSRLNY PENGWTPGED SYKEWIQVDL GLLRFVTAVG TQGAISKETK KKYYVKTYRV 

       370        380        390        400        410        420 
DISSNGEDWI SLKEGNKAII FQGNTNPTDV VLGVFSKPLI TRFVRIKPVS WETGISMRFE 

       430        440        450        460        470        480 
VYGCKITDYP CSGMLGMVSG LISDSQITAS NQADRNWMPE NIRLVTSRTG WALPPSPHPY 

       490        500        510        520        530        540 
TNEWLQVDLG DEKIVRGVII QGGKHRENKV FMRKFKIAYS NNGSDWKTIM DDSKRKAKSF 

       550        560        570        580        590        600 
EGNNNYDTPE LRTFSPLSTR FIRIYPERAT HSGLGLRMEL LGCEVEAPTA GPTTPNGNPV 

       610        620        630        640        650        660 
DECDDDQANC HSGTGDDFQL TGGTTVLATE KPTIIDSTIQ SEFPTYGFNC EFGWGSHKTF 

       670        680        690        700        710        720 
CHWEHDSHAQ LRWSVLTSKT GPIQDHTGDG NFIYSQADEN QKGKVARLVS PVVYSQSSAH 

       730        740        750        760        770        780 
CMTFWYHMSG SHVGTLRVKL RYQKPEEYDQ LVWMVVGHQG DHWKEGRVLL HKSLKLYQVI 

       790        800        810        820        830        840 
FEGEIGKGNL GGIAVDDISI NNHISQEDCA KPTDLDKKNT EIKIDETGST PGYEGEGEGD 

       850        860        870        880        890        900 
KNISRKPGNV LKTLDPILIT IIAMSALGVL LGAVCGVVLY CACWHNGMSE RNLSALENYN 

       910        920 
FELVDGVKLK KDKLNPQSNY SEA 

« Hide

References

« Hide 'large scale' references
[1]"Developmentally regulated expression of a cell surface protein, neuropilin, in the mouse nervous system."
Kawakami A., Kitsukawa T., Takagi S., Fujisawa H.
J. Neurobiol. 29:1-17(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Embryonic brain.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[4]"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation."
Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.
Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-261 AND ASN-522.
Tissue: Myoblast.
[5]"Neuropilin 1 directly interacts with Fer kinase to mediate semaphorin 3A-induced death of cortical neurons."
Jiang S.X., Whitehead S., Aylsworth A., Slinn J., Zurakowski B., Chan K., Li J., Hou S.T.
J. Biol. Chem. 285:9908-9918(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FER.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50086 mRNA. Translation: BAA08789.1.
CH466525 Genomic DNA. Translation: EDL11828.1.
BC060129 mRNA. Translation: AAH60129.1.
CCDSCCDS22790.1.
RefSeqNP_032763.2. NM_008737.2.
XP_006530829.1. XM_006530766.1.
UniGeneMm.271745.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4GZ9X-ray2.70A22-586[»]
4GZAX-ray7.00H22-586[»]
ProteinModelPortalP97333.
SMRP97333. Positions 25-586, 681-801.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201848. 5 interactions.
DIPDIP-39360N.
IntActP97333. 12 interactions.
MINTMINT-99860.

PTM databases

PhosphoSiteP97333.

Proteomic databases

MaxQBP97333.
PaxDbP97333.
PRIDEP97333.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000026917; ENSMUSP00000026917; ENSMUSG00000025810.
GeneID18186.
KEGGmmu:18186.
UCSCuc009nzr.2. mouse.

Organism-specific databases

CTD8829.
MGIMGI:106206. Nrp1.

Phylogenomic databases

eggNOGNOG150941.
GeneTreeENSGT00740000115053.
HOGENOMHOG000039978.
HOVERGENHBG000502.
InParanoidQ6PAR3.
KOK06724.
OMAYCACWHN.
OrthoDBEOG7ZWD1D.
TreeFamTF316506.

Enzyme and pathway databases

ReactomeREACT_188576. Developmental Biology.
REACT_194409. Developmental Biology.

Gene expression databases

ArrayExpressP97333.
BgeeP97333.
CleanExMM_NRP1.
GenevestigatorP97333.

Family and domain databases

Gene3D2.60.120.260. 2 hits.
2.60.120.290. 2 hits.
InterProIPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR000859. CUB_dom.
IPR008979. Galactose-bd-like.
IPR000998. MAM_dom.
IPR014648. Neuropilin.
IPR022579. Neuropilin1_C.
IPR027146. NRP1.
[Graphical view]
PANTHERPTHR10127:SF616. PTHR10127:SF616. 1 hit.
PfamPF00431. CUB. 2 hits.
PF11980. DUF3481. 1 hit.
PF00754. F5_F8_type_C. 2 hits.
PF00629. MAM. 1 hit.
[Graphical view]
PIRSFPIRSF036960. Neuropilin. 1 hit.
PRINTSPR00020. MAMDOMAIN.
SMARTSM00042. CUB. 2 hits.
SM00231. FA58C. 2 hits.
SM00137. MAM. 1 hit.
[Graphical view]
SUPFAMSSF49785. SSF49785. 2 hits.
SSF49854. SSF49854. 2 hits.
SSF49899. SSF49899. 1 hit.
PROSITEPS01180. CUB. 2 hits.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSNRP1. mouse.
NextBio293506.
PROP97333.
SOURCESearch...

Entry information

Entry nameNRP1_MOUSE
AccessionPrimary (citable) accession number: P97333
Secondary accession number(s): Q6PAR3
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot