P97333 (NRP1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Neuropilin-1

Alternative name(s):
A5 protein
CD_antigen=CD304

Gene names

Name:	Nrp1
Synonyms:	Nrp

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	923 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis By similarity.
Subunit structure	Homodimer, and heterodimer with NRP2. Binds PLXNB1 By similarity. Interacts with FER. Ref.5
Subcellular location	Membrane; Single-pass type I membrane protein.
Tissue specificity	Nervous system.
Domain	The tandem CUB domains mediate binding to semaphorin, while the tandem F5/8 domains are responsible for heparin and VEGF binding By similarity.
Sequence similarities	Belongs to the neuropilin family. Contains 2 CUB domains. Contains 2 F5/8 type C domains. Contains 1 MAM domain.

Ontologies

Keywords
Biological process	Angiogenesis Differentiation Neurogenesis
Cellular component	Membrane
Domain	Repeat Signal Transmembrane Transmembrane helix
Ligand	Calcium Heparin-binding Metal-binding
Molecular function	Developmental protein Receptor
PTM	Disulfide bond Glycoprotein Heparan sulfate Proteoglycan
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	axon guidance Inferred from direct assay PubMed 15814794. Source: MGI axonal fasciculation Inferred from mutant phenotype PubMed 17428830. Source: MGI cell adhesion Inferred from electronic annotation. Source: InterPro cell migration Inferred from mutant phenotype PubMed 15376331. Source: MGI cellular response to hepatocyte growth factor stimulus Inferred from electronic annotation. Source: Compara dendrite development Inferred from mutant phenotype PubMed 12852851. Source: MGI dichotomous subdivision of terminal units involved in salivary gland branching Inferred from mutant phenotype PubMed 17626059. Source: MGI endothelial cell chemotaxis Inferred from electronic annotation. Source: Compara heart development Inferred from genetic interaction PubMed 12852851. Source: MGI hepatocyte growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara negative regulation of axon extension involved in axon guidance Inferred from direct assay PubMed 9753685. Source: MGI organ morphogenesis Inferred from electronic annotation. Source: InterPro patterning of blood vessels Inferred from mutant phenotype PubMed 17428830. Source: MGI platelet-derived growth factor receptor signaling pathway Inferred from electronic annotation. Source: Compara positive regulation of peptidyl-tyrosine phosphorylation Inferred from electronic annotation. Source: Compara response to wounding Inferred from electronic annotation. Source: Compara sympathetic nervous system development Inferred from mutant phenotype PubMed 15814794. Source: MGI trigeminal nerve morphogenesis Inferred from mutant phenotype PubMed 15814794. Source: MGI vascular endothelial growth factor receptor signaling pathway Inferred from mutant phenotype PubMed 12852851. Source: MGI
Cellular_component	cell surface Inferred from electronic annotation. Source: Compara cytosol Inferred from electronic annotation. Source: Compara growth cone Inferred from electronic annotation. Source: Compara integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neurofilament Inferred from direct assay PubMed 15814794. Source: MGI neuronal cell body Inferred from electronic annotation. Source: Compara plasma membrane Traceable author statement. Source: Reactome
Molecular_function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW semaphorin receptor activity Inferred from genetic interaction PubMed 12591607. Source: MGI vascular endothelial growth factor-activated receptor activity Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 21

21

Potential

Chain

22 – 923

902

Neuropilin-1

PRO_0000021860

Regions

Topological domain

22 – 856

835

Extracellular Potential

Transmembrane

857 – 879

23

Helical; Potential

Topological domain

880 – 923

44

Cytoplasmic Potential

Domain

27 – 141

115

CUB 1

Domain

147 – 265

119

CUB 2

Domain

275 – 424

150

F5/8 type C 1

Domain

431 – 583

153

F5/8 type C 2

Domain

645 – 811

167

MAM

Sites

Metal binding

195

1

Calcium By similarity

Metal binding

209

1

Calcium By similarity

Metal binding

250

1

Calcium By similarity

Amino acid modifications

Glycosylation

150

1

N-linked (GlcNAc...) Potential

Glycosylation

261

1

N-linked (GlcNAc...) Ref.4

Glycosylation

300

1

N-linked (GlcNAc...) Potential

Glycosylation

522

1

N-linked (GlcNAc...) Ref.4

Glycosylation

612

1

O-linked (Xyl...) (chondroitin sulfate); alternate By similarity

Glycosylation

612

1

O-linked (Xyl...) (heparan sulfate); alternate By similarity

Glycosylation

842

1

N-linked (GlcNAc...) Potential

Disulfide bond

27 ↔ 54

Probable

Disulfide bond

82 ↔ 104

Probable

Disulfide bond

147 ↔ 173

By similarity

Disulfide bond

206 ↔ 228

By similarity

Disulfide bond

275 ↔ 424

By similarity

Disulfide bond

431 ↔ 583

By similarity

Experimental info

Sequence conflict

68

1

M → I in BAA08789. Ref.1

Sequence conflict

170

1

S → C in BAA08789. Ref.1

Sequence conflict

601

1

D → H in BAA08789. Ref.1

Secondary structure

1

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923

Helix Strand Turn

Details...

Beta strand

27 – 32

6

Beta strand

37 – 40

4

Turn

42 – 46

5

Beta strand

53 – 59

7

Beta strand

67 – 71

5

Helix

80 – 82

3

Beta strand

84 – 94

11

Beta strand

97 – 103

7

Beta strand

105 – 107

3

Beta strand

115 – 124

10

Beta strand

135 – 140

6

Beta strand

146 – 151

6

Beta strand

153 – 159

7

Turn

161 – 164

4

Beta strand

172 – 178

7

Helix

180 – 182

3

Beta strand

185 – 193

9

Beta strand

208 – 217

10

Turn

218 – 220

3

Beta strand

223 – 227

5

Beta strand

229 – 231

3

Beta strand

236 – 238

3

Beta strand

240 – 248

9

Beta strand

257 – 264

8

Turn

281 – 283

3

Beta strand

284 – 286

3

Helix

288 – 290

3

Beta strand

291 – 294

4

Helix

299 – 301

3

Helix

303 – 305

3

Beta strand

326 – 342

17

Turn

347 – 349

3

Beta strand

352 – 368

17

Beta strand

373 – 378

6

Beta strand

385 – 389

5

Beta strand

391 – 414

24

Beta strand

417 – 424

8

Helix

426 – 428

3

Beta strand

429 – 431

3

Turn

437 – 439

3

Beta strand

440 – 442

3

Helix

444 – 446

3

Beta strand

447 – 449

3

Turn

450 – 453

4

Helix

459 – 462

4

Turn

464 – 466

3

Beta strand

471 – 473

3

Beta strand

485 – 501

17

Beta strand

503 – 507

5

Beta strand

512 – 525

14

Beta strand

534 – 537

4

Beta strand

544 – 547

4

Beta strand

550 – 570

21

Beta strand

573 – 583

11

Sequences

	Sequence		Length	Mass (Da)
	P97333 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 23209818C5C42137	FASTA	923	103,000
	10 20 30 40 50 60 MERGLPLLCA TLALALALAG AFRSDKCGGT IKIENPGYLT SPGYPHSYHP SEKCEWLIQA 70 80 90 100 110 120 PEPYQRIMIN FNPHFDLEDR DCKYDYVEVI DGENEGGRLW GKFCGKIAPS PVVSSGPFLF 130 140 150 160 170 180 IKFVSDYETH GAGFSIRYEI FKRGPECSQN YTAPTGVIKS PGFPEKYPNS LECTYIIFAP 190 200 210 220 230 240 KMSEIILEFE SFDLEQDSNP PGGMFCRYDR LEIWDGFPEV GPHIGRYCGQ KTPGRIRSSS 250 260 270 280 290 300 GVLSMVFYTD SAIAKEGFSA NYSVLQSSIS EDFKCMEALG MESGEIHSDQ ITASSQYGTN 310 320 330 340 350 360 WSVERSRLNY PENGWTPGED SYKEWIQVDL GLLRFVTAVG TQGAISKETK KKYYVKTYRV 370 380 390 400 410 420 DISSNGEDWI SLKEGNKAII FQGNTNPTDV VLGVFSKPLI TRFVRIKPVS WETGISMRFE 430 440 450 460 470 480 VYGCKITDYP CSGMLGMVSG LISDSQITAS NQADRNWMPE NIRLVTSRTG WALPPSPHPY 490 500 510 520 530 540 TNEWLQVDLG DEKIVRGVII QGGKHRENKV FMRKFKIAYS NNGSDWKTIM DDSKRKAKSF 550 560 570 580 590 600 EGNNNYDTPE LRTFSPLSTR FIRIYPERAT HSGLGLRMEL LGCEVEAPTA GPTTPNGNPV 610 620 630 640 650 660 DECDDDQANC HSGTGDDFQL TGGTTVLATE KPTIIDSTIQ SEFPTYGFNC EFGWGSHKTF 670 680 690 700 710 720 CHWEHDSHAQ LRWSVLTSKT GPIQDHTGDG NFIYSQADEN QKGKVARLVS PVVYSQSSAH 730 740 750 760 770 780 CMTFWYHMSG SHVGTLRVKL RYQKPEEYDQ LVWMVVGHQG DHWKEGRVLL HKSLKLYQVI 790 800 810 820 830 840 FEGEIGKGNL GGIAVDDISI NNHISQEDCA KPTDLDKKNT EIKIDETGST PGYEGEGEGD 850 860 870 880 890 900 KNISRKPGNV LKTLDPILIT IIAMSALGVL LGAVCGVVLY CACWHNGMSE RNLSALENYN 910 920 FELVDGVKLK KDKLNPQSNY SEA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Developmentally regulated expression of a cell surface protein, neuropilin, in the mouse nervous system." Kawakami A., Kitsukawa T., Takagi S., Fujisawa H. J. Neurobiol. 29:1-17(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Embryonic brain.
[2]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[4]	"The mouse C2C12 myoblast cell surface N-linked glycoproteome: identification, glycosite occupancy, and membrane orientation." Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D., Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B. Mol. Cell. Proteomics 8:2555-2569(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-261 AND ASN-522, MASS SPECTROMETRY. Tissue: Myoblast.
[5]	"Neuropilin 1 directly interacts with Fer kinase to mediate semaphorin 3A-induced death of cortical neurons." Jiang S.X., Whitehead S., Aylsworth A., Slinn J., Zurakowski B., Chan K., Li J., Hou S.T. J. Biol. Chem. 285:9908-9918(2010) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FER.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D50086 mRNA. Translation: BAA08789.1.
CH466525 Genomic DNA. Translation: EDL11828.1.
BC060129 mRNA. Translation: AAH60129.1.

IPI

IPI00123996.

RefSeq

NP_032763.2. NM_008737.2.

UniGene

Mm.271745.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4GZ9	X-ray	2.70	A	22-586	[»]
4GZA	X-ray	7.00	H	22-586	[»]

ProteinModelPortal

P97333.

SMR

P97333. Positions 25-586, 648-804.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-39360N.

IntAct

P97333. 1 interaction.

MINT

MINT-99860.

PTM databases

PhosphoSite

P97333.

Proteomic databases

PaxDb

P97333.

PRIDE

P97333.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000026917; ENSMUSP00000026917; ENSMUSG00000025810.

GeneID

18186.

KEGG

mmu:18186.

Organism-specific databases

CTD

8829.

MGI

MGI:106206. Nrp1.

Phylogenomic databases

eggNOG

NOG150941.

GeneTree

ENSGT00670000097729.

HOGENOM

HOG000039978.

HOVERGEN

HBG000502.

InParanoid

Q6PAR3.

KO

K06724.

OMA

TYIIFAP.

OrthoDB

EOG4FN4H1.

Enzyme and pathway databases

Reactome

REACT_115433. Developmental Biology.
REACT_127416. Developmental Biology.

Gene expression databases

ArrayExpress

P97333.

Bgee

P97333.

CleanEx

MM_NRP1.

Genevestigator

P97333.

GermOnline

ENSMUSG00000025810. Mus musculus.

Family and domain databases

Gene3D

2.60.120.290. 2 hits.

InterPro

IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR008985. ConA-like_lec_gl_sf.
IPR000859. CUB_dom.
IPR008979. Galactose-bd-like.
IPR000998. MAM_dom.
IPR014648. Neuropilin.
IPR022579. Neuropilin1_C.
IPR027146. NRP1.
[Graphical view]

PANTHER

PTHR10127:SF84. PTHR10127:SF84. 1 hit.

Pfam

PF00431. CUB. 2 hits.
PF11980. DUF3481. 1 hit.
PF00754. F5_F8_type_C. 2 hits.
PF00629. MAM. 1 hit.
[Graphical view]

PIRSF

PIRSF036960. Neuropilin. 1 hit.

PRINTS

PR00020. MAMDOMAIN.

SMART

SM00042. CUB. 2 hits.
SM00231. FA58C. 2 hits.
SM00137. MAM. 1 hit.
[Graphical view]

SUPFAM

SSF49899. ConA_like_lec_gl. 1 hit.
SSF49854. CUB. 2 hits.
SSF49785. Gal_bind_like. 2 hits.

PROSITE

PS01180. CUB. 2 hits.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
PS00740. MAM_1. 1 hit.
PS50060. MAM_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

NRP1. mouse.

NextBio

293506.

SOURCE

Search...

Entry information

Entry name

NRP1_MOUSE

Accession

Primary (citable) accession number: P97333
Secondary accession number(s): Q6PAR3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 127 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program