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P97329 (KI20A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Kinesin-like protein KIF20A
Alternative name(s):
Kinesin-like protein 174
Rab6-interacting kinesin-like protein
Rabkinesin-6
Gene names
Name:Kif20a
Synonyms:Rab6kifl
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length887 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle By similarity. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility. Ref.1

Subcellular location

Golgi apparatus By similarity. Cytoplasmcytoskeletonspindle Probable Ref.1.

Tissue specificity

Ubiquitously expressed, with highest levels in spleen and testis. Ref.1

Post-translational modification

Phosphorylated by PLK1 at Ser-527 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle By similarity.

Sequence similarities

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

Contains 1 kinesin motor domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 887886Kinesin-like protein KIF20A
PRO_0000125461

Regions

Domain63 – 506444Kinesin motor
Nucleotide binding159 – 1668ATP By similarity
Region805 – 88783Globular Potential
Coiled coil559 – 804246 Potential

Amino acid modifications

Modified residue21N-acetylserine By similarity
Modified residue71Phosphoserine By similarity
Modified residue141Phosphoserine By similarity
Modified residue211Phosphoserine By similarity
Modified residue5271Phosphoserine; by PLK1 By similarity
Modified residue8231Phosphoserine By similarity
Modified residue8551Phosphothreonine By similarity
Modified residue8651Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
P97329 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 7143CCB261A4EA15

FASTA88799,876
        10         20         30         40         50         60 
MSHRILSPPA GLLSDEDVVD SPILESTAAD LRSVVRKDLL SDCSVISASL EDKQALLEDT 

        70         80         90        100        110        120 
SEKVKVYLRI RPFLTSELDR QEDQGCVCIE NTETLVLQAP KDSFALKSNE RGVGQATHKF 

       130        140        150        160        170        180 
TFSQIFGPEV GQVAFFNLTM KEMVKDVLKG QNWLIYTYGV TNSGKTYTIQ GTSKDAGILP 

       190        200        210        220        230        240 
QSLALIFNSL QGQLHPTPDL KPLLSNEVIW LDSKQIRQEE MKKLSLLIGG LQEEELSTSV 

       250        260        270        280        290        300 
KKRVHTESRI GASNSFDSGV AGLSSTSQFT SSSQLDETSQ LWAQPDTVPV SVPADIRFSV 

       310        320        330        340        350        360 
WISFFEIYNE LLYDLLEPPS HQHKRQTLRL CEDQNGNPYV KDLNWIHVRD VEEAWKLLKV 

       370        380        390        400        410        420 
GRKNQSFAST HMNQQSSRSH SIFSIRILHL QGEGDIVPKI SELSLCDLAG SERCKHQKSG 

       430        440        450        460        470        480 
ERLKEAGNIN TSLHTLGRCI AALRQNQQNR SKQNLIPFRD SKLTRVFQGF FTGRGRSCMI 

       490        500        510        520        530        540 
VNVNPCASTY DETLHAAKFS ALASQLVHAP PVHLGIPSLH SFIKKHSPQV GPGLEKEDKA 

       550        560        570        580        590        600 
DSDLEDSPED EADVSVYGKE ELLQVVEAMK ALLLKERQEK LQLEIQLREE ICNEMVEQMQ 

       610        620        630        640        650        660 
QREQWCSERL DNQKELMEEL YEEKLKILKE SLTTFYQEQI QERDEKIEEL ETLLQEAKQQ 

       670        680        690        700        710        720 
PAAQQSGGLS LLRRSQRLAA SASTQQFQEV KAELEQCKTE LSSTTAELHK YQQVLKPPPP 

       730        740        750        760        770        780 
AKPFTIDVDK KLEEGQKNIR LLRTELQKLG QSLQSAERAC CHSTGAGKLR QALTNCDDIL 

       790        800        810        820        830        840 
IKQNQTLAEL QNNMVLVKLD LQKKAACIAE QYHTVLKLQG QASAKKRLGA NQENQQPNHQ 

       850        860        870        880 
PPGKKPFLRN LLPRTPTCQS STDSSPYARI LRSRHSPLLK SPFGKKY 

« Hide

References

« Hide 'large scale' references
[1]"Interaction of a Golgi-associated kinesin-like protein with Rab6."
Echard A., Jollivet F., Martinez O., Lacapere J.-J., Rousselet A., Janoueix-Lerosey I., Goud B.
Science 279:580-585(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Strain: BALB/c.
Tissue: Testis.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Heart and Lung.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09632 mRNA. Translation: CAA70845.1.
AK083412 mRNA. Translation: BAC38906.1.
AK084732 mRNA. Translation: BAC39267.1.
AK144844 mRNA. Translation: BAE26095.1.
BC060608 mRNA. Translation: AAH60608.1.
CCDSCCDS29131.1.
RefSeqNP_001159878.1. NM_001166406.1.
NP_001159879.1. NM_001166407.1.
NP_033030.1. NM_009004.4.
UniGeneMm.258846.

3D structure databases

ProteinModelPortalP97329.
SMRP97329. Positions 59-541.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202552. 2 interactions.
IntActP97329. 1 interaction.
STRING10090.ENSMUSP00000003877.

PTM databases

PhosphoSiteP97329.

Proteomic databases

MaxQBP97329.
PaxDbP97329.
PRIDEP97329.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000166044; ENSMUSP00000132659; ENSMUSG00000003779.
ENSMUST00000167161; ENSMUSP00000130045; ENSMUSG00000003779.
GeneID19348.
KEGGmmu:19348.
UCSCuc008ela.2. mouse.

Organism-specific databases

CTD10112.
MGIMGI:1201682. Kif20a.

Phylogenomic databases

eggNOGCOG5059.
GeneTreeENSGT00530000063172.
HOGENOMHOG000054207.
HOVERGENHBG052246.
InParanoidP97329.
KOK10402.
OMARLCEDQN.
OrthoDBEOG74BJR9.
PhylomeDBP97329.
TreeFamTF105232.

Gene expression databases

BgeeP97329.
CleanExMM_KIF20A.
GenevestigatorP97329.

Family and domain databases

Gene3D3.40.850.10. 2 hits.
InterProIPR027326. KIF20A.
IPR027640. Kinesin-like_fam.
IPR019821. Kinesin_motor_CS.
IPR001752. Kinesin_motor_dom.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR24115. PTHR24115. 1 hit.
PTHR24115:SF352. PTHR24115:SF352. 1 hit.
PfamPF00225. Kinesin. 1 hit.
[Graphical view]
PRINTSPR00380. KINESINHEAVY.
SMARTSM00129. KISc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 2 hits.
PROSITEPS00411. KINESIN_MOTOR_1. 1 hit.
PS50067. KINESIN_MOTOR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio296373.
PROP97329.
SOURCESearch...

Entry information

Entry nameKI20A_MOUSE
AccessionPrimary (citable) accession number: P97329
Secondary accession number(s): Q542M4
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot