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P97329

- KI20A_MOUSE

UniProt

P97329 - KI20A_MOUSE

Protein

Kinesin-like protein KIF20A

Gene

Kif20a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle By similarity. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.By similarity1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi159 – 1668ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. microtubule motor activity Source: InterPro

    GO - Biological processi

    1. cytokinesis Source: UniProtKB
    2. microtubule-based movement Source: InterPro
    3. microtubule bundle formation Source: UniProtKB
    4. protein transport Source: UniProtKB-KW

    Keywords - Molecular functioni

    Motor protein

    Keywords - Biological processi

    Protein transport, Transport

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Kinesin-like protein KIF20A
    Alternative name(s):
    Kinesin-like protein 174
    Rab6-interacting kinesin-like protein
    Rabkinesin-6
    Gene namesi
    Name:Kif20a
    Synonyms:Rab6kifl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:1201682. Kif20a.

    Subcellular locationi

    Golgi apparatus By similarity. Cytoplasmcytoskeletonspindle 1 Publication

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB-SubCell
    2. kinesin complex Source: InterPro
    3. microtubule Source: UniProtKB-KW
    4. spindle Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Golgi apparatus, Microtubule

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 887886Kinesin-like protein KIF20APRO_0000125461Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Modified residuei7 – 71PhosphoserineBy similarity
    Modified residuei14 – 141PhosphoserineBy similarity
    Modified residuei21 – 211PhosphoserineBy similarity
    Modified residuei527 – 5271Phosphoserine; by PLK1By similarity
    Modified residuei823 – 8231PhosphoserineBy similarity
    Modified residuei855 – 8551PhosphothreonineBy similarity
    Modified residuei865 – 8651PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by PLK1 at Ser-527 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP97329.
    PaxDbiP97329.
    PRIDEiP97329.

    PTM databases

    PhosphoSiteiP97329.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed, with highest levels in spleen and testis.1 Publication

    Gene expression databases

    BgeeiP97329.
    CleanExiMM_KIF20A.
    GenevestigatoriP97329.

    Interactioni

    Protein-protein interaction databases

    BioGridi202552. 2 interactions.
    IntActiP97329. 1 interaction.
    STRINGi10090.ENSMUSP00000003877.

    Structurei

    3D structure databases

    ProteinModelPortaliP97329.
    SMRiP97329. Positions 59-541.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini63 – 506444Kinesin motorPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni805 – 88783GlobularSequence AnalysisAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili559 – 804246Sequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.PROSITE-ProRule annotation
    Contains 1 kinesin motor domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiCOG5059.
    GeneTreeiENSGT00530000063172.
    HOGENOMiHOG000054207.
    HOVERGENiHBG052246.
    InParanoidiP97329.
    KOiK10402.
    OMAiRLCEDQN.
    OrthoDBiEOG74BJR9.
    PhylomeDBiP97329.
    TreeFamiTF105232.

    Family and domain databases

    Gene3Di3.40.850.10. 2 hits.
    InterProiIPR027326. KIF20A.
    IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR24115. PTHR24115. 1 hit.
    PTHR24115:SF352. PTHR24115:SF352. 1 hit.
    PfamiPF00225. Kinesin. 1 hit.
    [Graphical view]
    PRINTSiPR00380. KINESINHEAVY.
    SMARTiSM00129. KISc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 2 hits.
    PROSITEiPS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97329-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHRILSPPA GLLSDEDVVD SPILESTAAD LRSVVRKDLL SDCSVISASL    50
    EDKQALLEDT SEKVKVYLRI RPFLTSELDR QEDQGCVCIE NTETLVLQAP 100
    KDSFALKSNE RGVGQATHKF TFSQIFGPEV GQVAFFNLTM KEMVKDVLKG 150
    QNWLIYTYGV TNSGKTYTIQ GTSKDAGILP QSLALIFNSL QGQLHPTPDL 200
    KPLLSNEVIW LDSKQIRQEE MKKLSLLIGG LQEEELSTSV KKRVHTESRI 250
    GASNSFDSGV AGLSSTSQFT SSSQLDETSQ LWAQPDTVPV SVPADIRFSV 300
    WISFFEIYNE LLYDLLEPPS HQHKRQTLRL CEDQNGNPYV KDLNWIHVRD 350
    VEEAWKLLKV GRKNQSFAST HMNQQSSRSH SIFSIRILHL QGEGDIVPKI 400
    SELSLCDLAG SERCKHQKSG ERLKEAGNIN TSLHTLGRCI AALRQNQQNR 450
    SKQNLIPFRD SKLTRVFQGF FTGRGRSCMI VNVNPCASTY DETLHAAKFS 500
    ALASQLVHAP PVHLGIPSLH SFIKKHSPQV GPGLEKEDKA DSDLEDSPED 550
    EADVSVYGKE ELLQVVEAMK ALLLKERQEK LQLEIQLREE ICNEMVEQMQ 600
    QREQWCSERL DNQKELMEEL YEEKLKILKE SLTTFYQEQI QERDEKIEEL 650
    ETLLQEAKQQ PAAQQSGGLS LLRRSQRLAA SASTQQFQEV KAELEQCKTE 700
    LSSTTAELHK YQQVLKPPPP AKPFTIDVDK KLEEGQKNIR LLRTELQKLG 750
    QSLQSAERAC CHSTGAGKLR QALTNCDDIL IKQNQTLAEL QNNMVLVKLD 800
    LQKKAACIAE QYHTVLKLQG QASAKKRLGA NQENQQPNHQ PPGKKPFLRN 850
    LLPRTPTCQS STDSSPYARI LRSRHSPLLK SPFGKKY 887
    Length:887
    Mass (Da):99,876
    Last modified:May 1, 1997 - v1
    Checksum:i7143CCB261A4EA15
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09632 mRNA. Translation: CAA70845.1.
    AK083412 mRNA. Translation: BAC38906.1.
    AK084732 mRNA. Translation: BAC39267.1.
    AK144844 mRNA. Translation: BAE26095.1.
    BC060608 mRNA. Translation: AAH60608.1.
    CCDSiCCDS29131.1.
    RefSeqiNP_001159878.1. NM_001166406.1.
    NP_001159879.1. NM_001166407.1.
    NP_033030.1. NM_009004.4.
    UniGeneiMm.258846.

    Genome annotation databases

    EnsembliENSMUST00000166044; ENSMUSP00000132659; ENSMUSG00000003779.
    ENSMUST00000167161; ENSMUSP00000130045; ENSMUSG00000003779.
    GeneIDi19348.
    KEGGimmu:19348.
    UCSCiuc008ela.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09632 mRNA. Translation: CAA70845.1 .
    AK083412 mRNA. Translation: BAC38906.1 .
    AK084732 mRNA. Translation: BAC39267.1 .
    AK144844 mRNA. Translation: BAE26095.1 .
    BC060608 mRNA. Translation: AAH60608.1 .
    CCDSi CCDS29131.1.
    RefSeqi NP_001159878.1. NM_001166406.1.
    NP_001159879.1. NM_001166407.1.
    NP_033030.1. NM_009004.4.
    UniGenei Mm.258846.

    3D structure databases

    ProteinModelPortali P97329.
    SMRi P97329. Positions 59-541.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202552. 2 interactions.
    IntActi P97329. 1 interaction.
    STRINGi 10090.ENSMUSP00000003877.

    PTM databases

    PhosphoSitei P97329.

    Proteomic databases

    MaxQBi P97329.
    PaxDbi P97329.
    PRIDEi P97329.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000166044 ; ENSMUSP00000132659 ; ENSMUSG00000003779 .
    ENSMUST00000167161 ; ENSMUSP00000130045 ; ENSMUSG00000003779 .
    GeneIDi 19348.
    KEGGi mmu:19348.
    UCSCi uc008ela.2. mouse.

    Organism-specific databases

    CTDi 10112.
    MGIi MGI:1201682. Kif20a.

    Phylogenomic databases

    eggNOGi COG5059.
    GeneTreei ENSGT00530000063172.
    HOGENOMi HOG000054207.
    HOVERGENi HBG052246.
    InParanoidi P97329.
    KOi K10402.
    OMAi RLCEDQN.
    OrthoDBi EOG74BJR9.
    PhylomeDBi P97329.
    TreeFami TF105232.

    Miscellaneous databases

    NextBioi 296373.
    PROi P97329.
    SOURCEi Search...

    Gene expression databases

    Bgeei P97329.
    CleanExi MM_KIF20A.
    Genevestigatori P97329.

    Family and domain databases

    Gene3Di 3.40.850.10. 2 hits.
    InterProi IPR027326. KIF20A.
    IPR027640. Kinesin-like_fam.
    IPR019821. Kinesin_motor_CS.
    IPR001752. Kinesin_motor_dom.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR24115. PTHR24115. 1 hit.
    PTHR24115:SF352. PTHR24115:SF352. 1 hit.
    Pfami PF00225. Kinesin. 1 hit.
    [Graphical view ]
    PRINTSi PR00380. KINESINHEAVY.
    SMARTi SM00129. KISc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 2 hits.
    PROSITEi PS00411. KINESIN_MOTOR_1. 1 hit.
    PS50067. KINESIN_MOTOR_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Interaction of a Golgi-associated kinesin-like protein with Rab6."
      Echard A., Jollivet F., Martinez O., Lacapere J.-J., Rousselet A., Janoueix-Lerosey I., Goud B.
      Science 279:580-585(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Strain: BALB/c.
      Tissue: Testis.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Heart and Lung.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiKI20A_MOUSE
    AccessioniPrimary (citable) accession number: P97329
    Secondary accession number(s): Q542M4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3