Reviewed, UniProtKB/Swiss-Prot P97328 (KHK_MOUSE)

Names and origin

Protein names

Recommended name:
    Ketohexokinase
    EC=2.7.1.3
Alternative name(s):
    Hepatic fructokinase

Gene names

Name:

Khk

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + D-fructose = ADP + D-fructose 1-phosphate.
Enzyme regulation	Requires potassium. Inhibition by ADP By similarity.
Pathway	Carbohydrate metabolism; fructose metabolism.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ketohexokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 298	298	Ketohexokinase		PRO_0000080089
Regions
	Nucleotide binding	255 – 258	4	ATP By similarity
Sites
	Binding site	15	1	D-fructose By similarity
	Binding site	41	1	D-fructose; via amide nitrogen By similarity
	Binding site	42	1	D-fructose By similarity
	Binding site	45	1	D-fructose By similarity
	Binding site	108	1	ATP By similarity
	Binding site	258	1	D-fructose By similarity
Amino acid modifications
	Modified residue	169	1	Phosphoserine Ref.3
Experimental info
	Sequence conflict	203	1	P → S in AAH13464. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P97328-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: A8181D781A6136D4	FASTA	298	32,750
	10 20 30 40 50 60 MEEKQILCVG LVVLDIINVV DKYPEEDTDR RCLSQRWQRG GNASNSCTVL SLLGARCAFM 70 80 90 100 110 120 GSLAPGHVAD FLVADFRQRG VDVSQVTWQS QGDTPCSCCI VNNSNGSRTI ILYDTNLPDV 130 140 150 160 170 180 SAKDFEKVDL TRFKWIHIEG RNASEQVKML QRIEEHNAKQ PLPQKVRVSV EIEKPREELF 190 200 210 220 230 240 QLFSYGEVVF VSKDVAKHLG FQPAVEALRG LYSRVKKGAT LVCAWAEEGA DALGPDGQLL 250 260 270 280 290 HSDAFPPPRV VDTLGAGDTF NASVIFSLSK GNSMQEALRF GCQVAGKKCG LQGFDGIV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure and function of the fructokinase gene." Hayward B.E., Bonthron D.T. Submitted (NOV-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.
[3]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-169, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y09335 mRNA. Translation: CAA70515.1. BC013464 mRNA. Translation: AAH13464.1.
IPI	IPI00331680.
RefSeq	NP_032465.2.
UniGene	Mm.22451
3D structure databases
SMR	P97328. Positions 5-298.
ModBase	Search...
Protein-protein interaction databases
STRING	P97328.
PTM databases
PhosphoSite	P97328.
2-D gel databases
SWISS-2DPAGE	P97328.
Proteomic databases
PRIDE	P97328.
Genome annotation databases
Ensembl	ENSMUST00000031053; ENSMUSP00000031053; ENSMUSG00000029162; Mus musculus. [Genome view] ENSMUST00000117435; ENSMUSP00000114064; ENSMUSG00000029162; Mus musculus. [Genome view]
GeneID	16548.
KEGG	mmu:16548.
UCSC	uc008wwn.1. mouse.
Organism-specific databases
MGI	MGI:1096353. Khk.
Phylogenomic databases
HOGENOM	P97328.
HOVERGEN	P97328.
Enzyme and pathway databases
BRENDA	2.7.1.3. 244.
Gene expression databases
ArrayExpress	P97328.
Bgee	P97328.
CleanEx	MM_KHK.
Genevestigator	P97328.
GermOnline	ENSMUSG00000029162. Mus musculus.
Family and domain databases
InterPro	IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. [Graphical view]
Pfam	PF00294. PfkB. 2 hits. [Graphical view]
PROSITE	PS00583. PFKB_KINASES_1. False negative. PS00584. PFKB_KINASES_2. False negative. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

KHK_MOUSE

Accession

Primary (citable) accession number: P97328
Secondary accession number(s): Q91WU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	May 1, 1997
Last modified:	October 13, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)