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P97325

- SIAT6_MOUSE

UniProt

P97325 - SIAT6_MOUSE

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Protein

CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase

Gene

St3gal3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein.

Pathwayi

GO - Molecular functioni

  1. beta-galactoside (CMP) alpha-2,3-sialyltransferase activity Source: MGI
  2. N-acetyllactosaminide alpha-2,3-sialyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: MGI
  2. sialylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.4. 3474.
ReactomeiREACT_196517. Pre-NOTCH Processing in Golgi.
REACT_198575. Termination of O-glycan biosynthesis.
REACT_198578. Keratan sulfate biosynthesis.
REACT_202500. Sialic acid metabolism.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase (EC:2.4.99.6)
Alternative name(s):
Beta-galactoside alpha-2,3-sialyltransferase 3
Short name:
Alpha 2,3-ST 3
Gal beta-1,3(4) GlcNAc alpha-2,3 sialyltransferase
N-acetyllactosaminide alpha-2,3-sialyltransferase
ST3Gal III
Short name:
ST3GalIII
ST3N
Sialyltransferase 6
Gene namesi
Name:St3gal3
Synonyms:Siat3, Siat6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1316659. St3gal3.

Subcellular locationi

GO - Cellular componenti

  1. integral component of Golgi membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferasePRO_0000149267Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi159 ↔ 313By similarity
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP97325.

PTM databases

PhosphoSiteiP97325.

Expressioni

Tissue specificityi

Found in all tissues tested. High expression found in brain, liver, kidney, colon, heart and spleen.

Developmental stagei

Developmental regulation only occurs in liver, heart, kidney and spleen.

Gene expression databases

BgeeiP97325.
ExpressionAtlasiP97325. baseline and differential.
GenevestigatoriP97325.

Structurei

3D structure databases

ProteinModelPortaliP97325.
SMRiP97325. Positions 110-338.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Topological domaini29 – 374346LumenalSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei9 – 2820Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257001.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000000682.
HOVERGENiHBG056676.
InParanoidiP97325.
KOiK00781.
OMAiLSCKRCI.
OrthoDBiEOG76X609.
TreeFamiTF354325.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

P97325-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLLVFVRNL LLALCLFLVL GFLYYSAWKL HLLQWEDSNS LLLSLDSAGQ
60 70 80 90 100
TLGTEYDRLG FLLKLDSKLP AELATKYANF SEGACKPGYA SAMMTAIFPR
110 120 130 140 150
FSKPAPMFLD DSFRKWARIR EFVPPFGIKG QDNLIKAILS VTKEYRLTPA
160 170 180 190 200
LDSLHCRRCI IVGNGGVLAN KSLGSRIDDY DIVIRLNSAP VKGFERDVGS
210 220 230 240 250
KTTLRITYPE GAMQRPEQYE RDSLFVLAGF KWQDFKWLKY IVYKERVSAS
260 270 280 290 300
DGFWKSVATR VPKEPPEIRI LNPYFIQEAA FTLIGLPFNN GLMGRGNIPT
310 320 330 340 350
LGSVAVTMAL HGCDEVAVAG FGYDMNTPNA PLHYYETVRM AAIKESWTHN
360 370
IQREKEFLRK LVKARVITDL SSGI
Length:374
Mass (Da):42,132
Last modified:July 27, 2011 - v2
Checksum:i9E776305912CECDC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781A → P in CAA59013. (PubMed:9184827)Curated
Sequence conflicti350 – 3501N → S in CAA59013. (PubMed:9184827)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84234 mRNA. Translation: CAA59013.1.
AL627128, AL611952, AL626764 Genomic DNA. Translation: CAM14710.1.
AL611952, AL626764, AL627128 Genomic DNA. Translation: CAM26721.1.
AL626764, AL611952, AL627128 Genomic DNA. Translation: CAM27383.1.
CH466552 Genomic DNA. Translation: EDL30520.1.
CH466552 Genomic DNA. Translation: EDL30522.1.
BC006710 mRNA. Translation: AAH06710.1.
CCDSiCCDS18544.1.
RefSeqiNP_001155246.1. NM_001161774.2.
NP_001272449.1. NM_001285520.1.
NP_033202.3. NM_009176.5.
UniGeneiMm.251002.

Genome annotation databases

EnsembliENSMUST00000030263; ENSMUSP00000030263; ENSMUSG00000028538.
ENSMUST00000106410; ENSMUSP00000102018; ENSMUSG00000028538.
GeneIDi20441.
KEGGimmu:20441.
UCSCiuc008ujk.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST3Gal III

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X84234 mRNA. Translation: CAA59013.1 .
AL627128 , AL611952 , AL626764 Genomic DNA. Translation: CAM14710.1 .
AL611952 , AL626764 , AL627128 Genomic DNA. Translation: CAM26721.1 .
AL626764 , AL611952 , AL627128 Genomic DNA. Translation: CAM27383.1 .
CH466552 Genomic DNA. Translation: EDL30520.1 .
CH466552 Genomic DNA. Translation: EDL30522.1 .
BC006710 mRNA. Translation: AAH06710.1 .
CCDSi CCDS18544.1.
RefSeqi NP_001155246.1. NM_001161774.2.
NP_001272449.1. NM_001285520.1.
NP_033202.3. NM_009176.5.
UniGenei Mm.251002.

3D structure databases

ProteinModelPortali P97325.
SMRi P97325. Positions 110-338.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSitei P97325.

Proteomic databases

PRIDEi P97325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030263 ; ENSMUSP00000030263 ; ENSMUSG00000028538 .
ENSMUST00000106410 ; ENSMUSP00000102018 ; ENSMUSG00000028538 .
GeneIDi 20441.
KEGGi mmu:20441.
UCSCi uc008ujk.2. mouse.

Organism-specific databases

CTDi 6487.
MGIi MGI:1316659. St3gal3.

Phylogenomic databases

eggNOGi NOG257001.
GeneTreei ENSGT00760000119095.
HOGENOMi HOG000000682.
HOVERGENi HBG056676.
InParanoidi P97325.
KOi K00781.
OMAi LSCKRCI.
OrthoDBi EOG76X609.
TreeFami TF354325.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BRENDAi 2.4.99.4. 3474.
Reactomei REACT_196517. Pre-NOTCH Processing in Golgi.
REACT_198575. Termination of O-glycan biosynthesis.
REACT_198578. Keratan sulfate biosynthesis.
REACT_202500. Sialic acid metabolism.

Miscellaneous databases

NextBioi 298480.
PROi P97325.
SOURCEi Search...

Gene expression databases

Bgeei P97325.
ExpressionAtlasi P97325. baseline and differential.
Genevestigatori P97325.

Family and domain databases

InterProi IPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view ]
Pfami PF00777. Glyco_transf_29. 1 hit.
[Graphical view ]
PIRSFi PIRSF005557. Sialyl_trans. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse beta-galactoside alpha2,3-sialyltransferases: comparison of in vitro substrate specificities and tissue specific expression."
    Kono M., Ohyama Y., Lee Y.-C., Hamamoto T., Kojima N., Tsuji S.
    Glycobiology 7:469-479(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain and Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiSIAT6_MOUSE
AccessioniPrimary (citable) accession number: P97325
Secondary accession number(s): Q922X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3