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Protein

CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase

Gene

St3gal3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- and NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.1 Publication

Catalytic activityi

CMP-N-acetyl-beta-neuraminate + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R = CMP + N-acetyl-alpha-neuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminyl-R.1 Publication

Kineticsi

Relative Vmax is 4:2:1 for Gal-beta-1,3-GlcNAc, Gal-beta-1,4-GlcNAc and Gal-beta-1,3-GalNAc as substrate, respectively.1 Publication
  1. KM=0.32 mM for Gal-beta-1,3-GlcNAc1 Publication
  2. KM=3.0 mM for Gal-beta-1,4-GlcNAc1 Publication
  3. KM=2.2 mM for Gal-beta-1,3-GalNAc1 Publication

    pH dependencei

    Optimum pH is 6.4.1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.99.4. 3474.
    2.4.99.6. 3474.
    2.4.99.8. 3474.
    ReactomeiR-MMU-2022854. Keratan sulfate biosynthesis.
    R-MMU-4085001. Sialic acid metabolism.
    R-MMU-977068. Termination of O-glycan biosynthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT29. Glycosyltransferase Family 29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase (EC:2.4.99.61 Publication)
    Alternative name(s):
    Beta-galactoside alpha-2,3-sialyltransferase 3
    Short name:
    Alpha 2,3-ST 3
    Gal beta-1,3(4) GlcNAc alpha-2,3 sialyltransferase
    N-acetyllactosaminide alpha-2,3-sialyltransferase
    ST3Gal III
    Short name:
    ST3GalIII
    ST3N
    Sialyltransferase 6
    Gene namesi
    Name:St3gal3
    Synonyms:Siat3, Siat6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 4

    Organism-specific databases

    MGIiMGI:1316659. St3gal3.

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 8CytoplasmicSequence analysis8
    Transmembranei9 – 28Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST20
    Topological domaini29 – 374LumenalSequence analysisAdd BLAST346

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001492671 – 374CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferaseAdd BLAST374

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi159 ↔ 313By similarity
    Glycosylationi170N-linked (GlcNAc...) asparagineSequence analysis1

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP97325.
    PaxDbiP97325.
    PRIDEiP97325.

    PTM databases

    iPTMnetiP97325.
    PhosphoSitePlusiP97325.

    Expressioni

    Tissue specificityi

    Found in all tissues tested. High expression found in brain, liver, kidney, colon, heart and spleen.1 Publication

    Developmental stagei

    Developmental regulation only occurs in liver, heart, kidney and spleen.

    Gene expression databases

    BgeeiENSMUSG00000028538.
    ExpressionAtlasiP97325. baseline and differential.
    GenevisibleiP97325. MM.

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000030263.

    Structurei

    3D structure databases

    ProteinModelPortaliP97325.
    SMRiP97325.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2692. Eukaryota.
    ENOG410XT8P. LUCA.
    GeneTreeiENSGT00760000119095.
    HOGENOMiHOG000000682.
    HOVERGENiHBG056676.
    InParanoidiP97325.
    KOiK00781.
    OMAiPHEIRIL.
    OrthoDBiEOG091G098L.
    TreeFamiTF354325.

    Family and domain databases

    InterProiView protein in InterPro
    IPR001675. Glyco_trans_29.
    IPR012163. Sialyl_trans.
    PfamiView protein in Pfam
    PF00777. Glyco_transf_29. 1 hit.
    PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P97325-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MGLLVFVRNL LLALCLFLVL GFLYYSAWKL HLLQWEDSNS LLLSLDSAGQ
    60 70 80 90 100
    TLGTEYDRLG FLLKLDSKLP AELATKYANF SEGACKPGYA SAMMTAIFPR
    110 120 130 140 150
    FSKPAPMFLD DSFRKWARIR EFVPPFGIKG QDNLIKAILS VTKEYRLTPA
    160 170 180 190 200
    LDSLHCRRCI IVGNGGVLAN KSLGSRIDDY DIVIRLNSAP VKGFERDVGS
    210 220 230 240 250
    KTTLRITYPE GAMQRPEQYE RDSLFVLAGF KWQDFKWLKY IVYKERVSAS
    260 270 280 290 300
    DGFWKSVATR VPKEPPEIRI LNPYFIQEAA FTLIGLPFNN GLMGRGNIPT
    310 320 330 340 350
    LGSVAVTMAL HGCDEVAVAG FGYDMNTPNA PLHYYETVRM AAIKESWTHN
    360 370
    IQREKEFLRK LVKARVITDL SSGI
    Length:374
    Mass (Da):42,132
    Last modified:July 27, 2011 - v2
    Checksum:i9E776305912CECDC
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti78A → P in CAA59013 (PubMed:9184827).Curated1
    Sequence conflicti350N → S in CAA59013 (PubMed:9184827).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    X84234 mRNA. Translation: CAA59013.1.
    AL627128, AL611952, AL626764 Genomic DNA. Translation: CAM14710.1.
    AL611952, AL626764, AL627128 Genomic DNA. Translation: CAM26721.1.
    AL626764, AL611952, AL627128 Genomic DNA. Translation: CAM27383.1.
    CH466552 Genomic DNA. Translation: EDL30520.1.
    CH466552 Genomic DNA. Translation: EDL30522.1.
    BC006710 mRNA. Translation: AAH06710.1.
    CCDSiCCDS18544.1.
    RefSeqiNP_001155246.1. NM_001161774.2.
    NP_001272449.1. NM_001285520.1.
    NP_033202.3. NM_009176.5.
    UniGeneiMm.251002.

    Genome annotation databases

    EnsembliENSMUST00000030263; ENSMUSP00000030263; ENSMUSG00000028538.
    ENSMUST00000106410; ENSMUSP00000102018; ENSMUSG00000028538.
    GeneIDi20441.
    KEGGimmu:20441.
    UCSCiuc008ujk.3. mouse.

    Similar proteinsi

    Entry informationi

    Entry nameiSIAT6_MOUSE
    AccessioniPrimary (citable) accession number: P97325
    Secondary accession number(s): Q922X5
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: July 27, 2011
    Last modified: December 20, 2017
    This is version 138 of the entry and version 2 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families