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P97325

- SIAT6_MOUSE

UniProt

P97325 - SIAT6_MOUSE

Protein

CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase

Gene

St3gal3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.

    Catalytic activityi

    CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein.

    Pathwayi

    GO - Molecular functioni

    1. beta-galactoside (CMP) alpha-2,3-sialyltransferase activity Source: MGI
    2. N-acetyllactosaminide alpha-2,3-sialyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: MGI
    2. sialylation Source: GOC

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Enzyme and pathway databases

    BRENDAi2.4.99.4. 3474.
    ReactomeiREACT_196517. Pre-NOTCH Processing in Golgi.
    REACT_198575. Termination of O-glycan biosynthesis.
    REACT_198578. Keratan sulfate biosynthesis.
    REACT_202500. Sialic acid metabolism.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT29. Glycosyltransferase Family 29.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase (EC:2.4.99.6)
    Alternative name(s):
    Beta-galactoside alpha-2,3-sialyltransferase 3
    Short name:
    Alpha 2,3-ST 3
    Gal beta-1,3(4) GlcNAc alpha-2,3 sialyltransferase
    N-acetyllactosaminide alpha-2,3-sialyltransferase
    ST3Gal III
    Short name:
    ST3GalIII
    ST3N
    Sialyltransferase 6
    Gene namesi
    Name:St3gal3
    Synonyms:Siat3, Siat6
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1316659. St3gal3.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of Golgi membrane Source: InterPro

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 374374CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferasePRO_0000149267Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi159 ↔ 313By similarity
    Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP97325.

    PTM databases

    PhosphoSiteiP97325.

    Expressioni

    Tissue specificityi

    Found in all tissues tested. High expression found in brain, liver, kidney, colon, heart and spleen.

    Developmental stagei

    Developmental regulation only occurs in liver, heart, kidney and spleen.

    Gene expression databases

    ArrayExpressiP97325.
    BgeeiP97325.
    GenevestigatoriP97325.

    Structurei

    3D structure databases

    ProteinModelPortaliP97325.
    SMRiP97325. Positions 110-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 88CytoplasmicSequence Analysis
    Topological domaini29 – 374346LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei9 – 2820Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 29 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG257001.
    GeneTreeiENSGT00620000087815.
    HOGENOMiHOG000000682.
    HOVERGENiHBG056676.
    KOiK00781.
    OMAiLSCKRCI.
    OrthoDBiEOG76X609.
    TreeFamiTF354325.

    Family and domain databases

    InterProiIPR001675. Glyco_trans_29.
    IPR012163. Sialyl_trans.
    [Graphical view]
    PfamiPF00777. Glyco_transf_29. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P97325-1 [UniParc]FASTAAdd to Basket

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    MGLLVFVRNL LLALCLFLVL GFLYYSAWKL HLLQWEDSNS LLLSLDSAGQ    50
    TLGTEYDRLG FLLKLDSKLP AELATKYANF SEGACKPGYA SAMMTAIFPR 100
    FSKPAPMFLD DSFRKWARIR EFVPPFGIKG QDNLIKAILS VTKEYRLTPA 150
    LDSLHCRRCI IVGNGGVLAN KSLGSRIDDY DIVIRLNSAP VKGFERDVGS 200
    KTTLRITYPE GAMQRPEQYE RDSLFVLAGF KWQDFKWLKY IVYKERVSAS 250
    DGFWKSVATR VPKEPPEIRI LNPYFIQEAA FTLIGLPFNN GLMGRGNIPT 300
    LGSVAVTMAL HGCDEVAVAG FGYDMNTPNA PLHYYETVRM AAIKESWTHN 350
    IQREKEFLRK LVKARVITDL SSGI 374
    Length:374
    Mass (Da):42,132
    Last modified:July 27, 2011 - v2
    Checksum:i9E776305912CECDC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti78 – 781A → P in CAA59013. (PubMed:9184827)Curated
    Sequence conflicti350 – 3501N → S in CAA59013. (PubMed:9184827)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84234 mRNA. Translation: CAA59013.1.
    AL627128, AL611952, AL626764 Genomic DNA. Translation: CAM14710.1.
    AL611952, AL626764, AL627128 Genomic DNA. Translation: CAM26721.1.
    AL626764, AL611952, AL627128 Genomic DNA. Translation: CAM27383.1.
    CH466552 Genomic DNA. Translation: EDL30520.1.
    CH466552 Genomic DNA. Translation: EDL30522.1.
    BC006710 mRNA. Translation: AAH06710.1.
    CCDSiCCDS18544.1.
    RefSeqiNP_001155246.1. NM_001161774.2.
    NP_001272449.1. NM_001285520.1.
    NP_033202.3. NM_009176.5.
    UniGeneiMm.251002.

    Genome annotation databases

    EnsembliENSMUST00000030263; ENSMUSP00000030263; ENSMUSG00000028538.
    ENSMUST00000106410; ENSMUSP00000102018; ENSMUSG00000028538.
    GeneIDi20441.
    KEGGimmu:20441.
    UCSCiuc008ujk.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    ST3Gal III

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X84234 mRNA. Translation: CAA59013.1 .
    AL627128 , AL611952 , AL626764 Genomic DNA. Translation: CAM14710.1 .
    AL611952 , AL626764 , AL627128 Genomic DNA. Translation: CAM26721.1 .
    AL626764 , AL611952 , AL627128 Genomic DNA. Translation: CAM27383.1 .
    CH466552 Genomic DNA. Translation: EDL30520.1 .
    CH466552 Genomic DNA. Translation: EDL30522.1 .
    BC006710 mRNA. Translation: AAH06710.1 .
    CCDSi CCDS18544.1.
    RefSeqi NP_001155246.1. NM_001161774.2.
    NP_001272449.1. NM_001285520.1.
    NP_033202.3. NM_009176.5.
    UniGenei Mm.251002.

    3D structure databases

    ProteinModelPortali P97325.
    SMRi P97325. Positions 110-338.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT29. Glycosyltransferase Family 29.

    PTM databases

    PhosphoSitei P97325.

    Proteomic databases

    PRIDEi P97325.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030263 ; ENSMUSP00000030263 ; ENSMUSG00000028538 .
    ENSMUST00000106410 ; ENSMUSP00000102018 ; ENSMUSG00000028538 .
    GeneIDi 20441.
    KEGGi mmu:20441.
    UCSCi uc008ujk.2. mouse.

    Organism-specific databases

    CTDi 6487.
    MGIi MGI:1316659. St3gal3.

    Phylogenomic databases

    eggNOGi NOG257001.
    GeneTreei ENSGT00620000087815.
    HOGENOMi HOG000000682.
    HOVERGENi HBG056676.
    KOi K00781.
    OMAi LSCKRCI.
    OrthoDBi EOG76X609.
    TreeFami TF354325.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    BRENDAi 2.4.99.4. 3474.
    Reactomei REACT_196517. Pre-NOTCH Processing in Golgi.
    REACT_198575. Termination of O-glycan biosynthesis.
    REACT_198578. Keratan sulfate biosynthesis.
    REACT_202500. Sialic acid metabolism.

    Miscellaneous databases

    NextBioi 298480.
    PROi P97325.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97325.
    Bgeei P97325.
    Genevestigatori P97325.

    Family and domain databases

    InterProi IPR001675. Glyco_trans_29.
    IPR012163. Sialyl_trans.
    [Graphical view ]
    Pfami PF00777. Glyco_transf_29. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005557. Sialyl_trans. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mouse beta-galactoside alpha2,3-sialyltransferases: comparison of in vitro substrate specificities and tissue specific expression."
      Kono M., Ohyama Y., Lee Y.-C., Hamamoto T., Kojima N., Tsuji S.
      Glycobiology 7:469-479(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain and Liver.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiSIAT6_MOUSE
    AccessioniPrimary (citable) accession number: P97325
    Secondary accession number(s): Q922X5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 27, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3