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Protein

CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase

Gene

St3gal3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.

Catalytic activityi

CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein.

Pathwayi

GO - Molecular functioni

  1. beta-galactoside (CMP) alpha-2,3-sialyltransferase activity Source: MGI
  2. N-acetyllactosaminide alpha-2,3-sialyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: MGI
  2. sialylation Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BRENDAi2.4.99.4. 3474.
2.4.99.6. 3474.
2.4.99.8. 3474.
ReactomeiREACT_278748. Termination of O-glycan biosynthesis.
REACT_310546. Sialic acid metabolism.
REACT_321348. Pre-NOTCH Processing in Golgi.
REACT_346228. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

Names & Taxonomyi

Protein namesi
Recommended name:
CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase (EC:2.4.99.6)
Alternative name(s):
Beta-galactoside alpha-2,3-sialyltransferase 3
Short name:
Alpha 2,3-ST 3
Gal beta-1,3(4) GlcNAc alpha-2,3 sialyltransferase
N-acetyllactosaminide alpha-2,3-sialyltransferase
ST3Gal III
Short name:
ST3GalIII
ST3N
Sialyltransferase 6
Gene namesi
Name:St3gal3
Synonyms:Siat3, Siat6
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1316659. St3gal3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 88CytoplasmicSequence Analysis
Transmembranei9 – 2820Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini29 – 374346LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of Golgi membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 374374CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferasePRO_0000149267Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi159 ↔ 313By similarity
Glycosylationi170 – 1701N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP97325.

PTM databases

PhosphoSiteiP97325.

Expressioni

Tissue specificityi

Found in all tissues tested. High expression found in brain, liver, kidney, colon, heart and spleen.

Developmental stagei

Developmental regulation only occurs in liver, heart, kidney and spleen.

Gene expression databases

BgeeiP97325.
ExpressionAtlasiP97325. baseline and differential.
GenevestigatoriP97325.

Structurei

3D structure databases

ProteinModelPortaliP97325.
SMRiP97325. Positions 110-338.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 29 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG257001.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000000682.
HOVERGENiHBG056676.
InParanoidiP97325.
KOiK00781.
OMAiPHEIRIL.
OrthoDBiEOG76X609.
TreeFamiTF354325.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.

Sequencei

Sequence statusi: Complete.

P97325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLVFVRNL LLALCLFLVL GFLYYSAWKL HLLQWEDSNS LLLSLDSAGQ
60 70 80 90 100
TLGTEYDRLG FLLKLDSKLP AELATKYANF SEGACKPGYA SAMMTAIFPR
110 120 130 140 150
FSKPAPMFLD DSFRKWARIR EFVPPFGIKG QDNLIKAILS VTKEYRLTPA
160 170 180 190 200
LDSLHCRRCI IVGNGGVLAN KSLGSRIDDY DIVIRLNSAP VKGFERDVGS
210 220 230 240 250
KTTLRITYPE GAMQRPEQYE RDSLFVLAGF KWQDFKWLKY IVYKERVSAS
260 270 280 290 300
DGFWKSVATR VPKEPPEIRI LNPYFIQEAA FTLIGLPFNN GLMGRGNIPT
310 320 330 340 350
LGSVAVTMAL HGCDEVAVAG FGYDMNTPNA PLHYYETVRM AAIKESWTHN
360 370
IQREKEFLRK LVKARVITDL SSGI
Length:374
Mass (Da):42,132
Last modified:July 27, 2011 - v2
Checksum:i9E776305912CECDC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti78 – 781A → P in CAA59013 (PubMed:9184827).Curated
Sequence conflicti350 – 3501N → S in CAA59013 (PubMed:9184827).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84234 mRNA. Translation: CAA59013.1.
AL627128, AL611952, AL626764 Genomic DNA. Translation: CAM14710.1.
AL611952, AL626764, AL627128 Genomic DNA. Translation: CAM26721.1.
AL626764, AL611952, AL627128 Genomic DNA. Translation: CAM27383.1.
CH466552 Genomic DNA. Translation: EDL30520.1.
CH466552 Genomic DNA. Translation: EDL30522.1.
BC006710 mRNA. Translation: AAH06710.1.
CCDSiCCDS18544.1.
RefSeqiNP_001155246.1. NM_001161774.2.
NP_001272449.1. NM_001285520.1.
NP_033202.3. NM_009176.5.
UniGeneiMm.251002.

Genome annotation databases

EnsembliENSMUST00000030263; ENSMUSP00000030263; ENSMUSG00000028538.
ENSMUST00000106410; ENSMUSP00000102018; ENSMUSG00000028538.
GeneIDi20441.
KEGGimmu:20441.
UCSCiuc008ujk.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

ST3Gal III

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X84234 mRNA. Translation: CAA59013.1.
AL627128, AL611952, AL626764 Genomic DNA. Translation: CAM14710.1.
AL611952, AL626764, AL627128 Genomic DNA. Translation: CAM26721.1.
AL626764, AL611952, AL627128 Genomic DNA. Translation: CAM27383.1.
CH466552 Genomic DNA. Translation: EDL30520.1.
CH466552 Genomic DNA. Translation: EDL30522.1.
BC006710 mRNA. Translation: AAH06710.1.
CCDSiCCDS18544.1.
RefSeqiNP_001155246.1. NM_001161774.2.
NP_001272449.1. NM_001285520.1.
NP_033202.3. NM_009176.5.
UniGeneiMm.251002.

3D structure databases

ProteinModelPortaliP97325.
SMRiP97325. Positions 110-338.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteiP97325.

Proteomic databases

PRIDEiP97325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000030263; ENSMUSP00000030263; ENSMUSG00000028538.
ENSMUST00000106410; ENSMUSP00000102018; ENSMUSG00000028538.
GeneIDi20441.
KEGGimmu:20441.
UCSCiuc008ujk.2. mouse.

Organism-specific databases

CTDi6487.
MGIiMGI:1316659. St3gal3.

Phylogenomic databases

eggNOGiNOG257001.
GeneTreeiENSGT00760000119095.
HOGENOMiHOG000000682.
HOVERGENiHBG056676.
InParanoidiP97325.
KOiK00781.
OMAiPHEIRIL.
OrthoDBiEOG76X609.
TreeFamiTF354325.

Enzyme and pathway databases

UniPathwayiUPA00378.
BRENDAi2.4.99.4. 3474.
2.4.99.6. 3474.
2.4.99.8. 3474.
ReactomeiREACT_278748. Termination of O-glycan biosynthesis.
REACT_310546. Sialic acid metabolism.
REACT_321348. Pre-NOTCH Processing in Golgi.
REACT_346228. Keratan sulfate biosynthesis.

Miscellaneous databases

ChiTaRSiSt3gal3. mouse.
NextBioi298480.
PROiP97325.
SOURCEiSearch...

Gene expression databases

BgeeiP97325.
ExpressionAtlasiP97325. baseline and differential.
GenevestigatoriP97325.

Family and domain databases

InterProiIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamiPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFiPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse beta-galactoside alpha2,3-sialyltransferases: comparison of in vitro substrate specificities and tissue specific expression."
    Kono M., Ohyama Y., Lee Y.-C., Hamamoto T., Kojima N., Tsuji S.
    Glycobiology 7:469-479(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain and Liver.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.

Entry informationi

Entry nameiSIAT6_MOUSE
AccessioniPrimary (citable) accession number: P97325
Secondary accession number(s): Q922X5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: April 1, 2015
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.