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P97325 (SIAT6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase

EC=2.4.99.6
Alternative name(s):
Beta-galactoside alpha-2,3-sialyltransferase 3
Short name=Alpha 2,3-ST 3
Gal beta-1,3(4) GlcNAc alpha-2,3 sialyltransferase
N-acetyllactosaminide alpha-2,3-sialyltransferase
ST3Gal III
Short name=ST3GalIII
ST3N
Sialyltransferase 6
Gene names
Name:St3gal3
Synonyms:Siat3, Siat6
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length374 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of the NeuAc-alpha-2,3-Gal-beta-1,4-GlcNAc-, NeuAc-alpha-2,3-Gal-beta-1,3-GlcNAc- or NeuAc-alpha-2,3-Gal-beta-1,3-GalNAc- sequences found in terminal carbohydrate groups of glycoproteins and glycolipids. The highest activity is toward Gal-beta-1,3-GlcNAc and the lowest toward Gal-beta-1,3-GalNAc.

Catalytic activity

CMP-N-acetylneuraminate + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein = CMP + alpha-N-acetylneuraminyl-(2->3)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-glycoprotein.

Pathway

Protein modification; protein glycosylation.

Subcellular location

Membrane; Single-pass type II membrane protein. Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein.

Tissue specificity

Found in all tissues tested. High expression found in brain, liver, kidney, colon, heart and spleen.

Developmental stage

Developmental regulation only occurs in liver, heart, kidney and spleen.

Sequence similarities

Belongs to the glycosyltransferase 29 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 374374CMP-N-acetylneuraminate-beta-1,4-galactoside alpha-2,3-sialyltransferase
PRO_0000149267

Regions

Topological domain1 – 88Cytoplasmic Potential
Transmembrane9 – 2820Helical; Signal-anchor for type II membrane protein; Potential
Topological domain29 – 374346Lumenal Potential

Amino acid modifications

Glycosylation791N-linked (GlcNAc...) Potential
Glycosylation1701N-linked (GlcNAc...) Potential
Disulfide bond159 ↔ 313 By similarity

Experimental info

Sequence conflict781A → P in CAA59013. Ref.1
Sequence conflict3501N → S in CAA59013. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P97325 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 9E776305912CECDC

FASTA37442,132
        10         20         30         40         50         60 
MGLLVFVRNL LLALCLFLVL GFLYYSAWKL HLLQWEDSNS LLLSLDSAGQ TLGTEYDRLG 

        70         80         90        100        110        120 
FLLKLDSKLP AELATKYANF SEGACKPGYA SAMMTAIFPR FSKPAPMFLD DSFRKWARIR 

       130        140        150        160        170        180 
EFVPPFGIKG QDNLIKAILS VTKEYRLTPA LDSLHCRRCI IVGNGGVLAN KSLGSRIDDY 

       190        200        210        220        230        240 
DIVIRLNSAP VKGFERDVGS KTTLRITYPE GAMQRPEQYE RDSLFVLAGF KWQDFKWLKY 

       250        260        270        280        290        300 
IVYKERVSAS DGFWKSVATR VPKEPPEIRI LNPYFIQEAA FTLIGLPFNN GLMGRGNIPT 

       310        320        330        340        350        360 
LGSVAVTMAL HGCDEVAVAG FGYDMNTPNA PLHYYETVRM AAIKESWTHN IQREKEFLRK 

       370 
LVKARVITDL SSGI 

« Hide

References

« Hide 'large scale' references
[1]"Mouse beta-galactoside alpha2,3-sialyltransferases: comparison of in vitro substrate specificities and tissue specific expression."
Kono M., Ohyama Y., Lee Y.-C., Hamamoto T., Kojima N., Tsuji S.
Glycobiology 7:469-479(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain and Liver.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X84234 mRNA. Translation: CAA59013.1.
AL627128, AL611952, AL626764 Genomic DNA. Translation: CAM14710.1.
AL611952, AL626764, AL627128 Genomic DNA. Translation: CAM26721.1.
AL626764, AL611952, AL627128 Genomic DNA. Translation: CAM27383.1.
CH466552 Genomic DNA. Translation: EDL30520.1.
CH466552 Genomic DNA. Translation: EDL30522.1.
BC006710 mRNA. Translation: AAH06710.1.
RefSeqNP_001155246.1. NM_001161774.2.
NP_001272449.1. NM_001285520.1.
NP_033202.3. NM_009176.5.
UniGeneMm.251002.

3D structure databases

ProteinModelPortalP97325.
SMRP97325. Positions 82-372.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGT29. Glycosyltransferase Family 29.

PTM databases

PhosphoSiteP97325.

Proteomic databases

PRIDEP97325.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000030263; ENSMUSP00000030263; ENSMUSG00000028538.
ENSMUST00000106410; ENSMUSP00000102018; ENSMUSG00000028538.
GeneID20441.
KEGGmmu:20441.
UCSCuc008ujk.2. mouse.

Organism-specific databases

CTD6487.
MGIMGI:1316659. St3gal3.

Phylogenomic databases

eggNOGNOG257001.
GeneTreeENSGT00620000087815.
HOGENOMHOG000000682.
HOVERGENHBG056676.
KOK00781.
OMALSCKRCI.
OrthoDBEOG76X609.
TreeFamTF354325.

Enzyme and pathway databases

BRENDA2.4.99.4. 3474.
UniPathwayUPA00378.

Gene expression databases

ArrayExpressP97325.
BgeeP97325.
GenevestigatorP97325.

Family and domain databases

InterProIPR001675. Glyco_trans_29.
IPR012163. Sialyl_trans.
[Graphical view]
PfamPF00777. Glyco_transf_29. 1 hit.
[Graphical view]
PIRSFPIRSF005557. Sialyl_trans. 1 hit.
ProtoNetSearch...

Other

NextBio298480.
PROP97325.
SOURCESearch...

Entry information

Entry nameSIAT6_MOUSE
AccessionPrimary (citable) accession number: P97325
Secondary accession number(s): Q922X5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot