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P97324 (G6PD2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucose-6-phosphate 1-dehydrogenase 2

Short name=G6PD
EC=1.1.1.49
Gene names
Name:G6pd2
Synonyms:G6pd-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length513 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the rate-limiting step of the oxidative pentose-phosphate pathway, which represents a route for the dissimilation of carbohydrates besides glycolysis. The main function of this enzyme is to provide reducing power (NADPH) and pentose phosphates for fatty acid and nucleic acid synthesis. Ref.1

Catalytic activity

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH. Ref.1

Pathway

Carbohydrate degradation; pentose phosphate pathway; D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage): step 1/3. HAMAP-Rule MF_00966

Subunit structure

Homotetramer; dimer of dimers. Ref.1

Tissue specificity

Testis. Ref.1

Miscellaneous

Has NADP both as cofactor (bound to the N-terminal domain) and as structural element bound to the C-terminal domain.

Sequence similarities

Belongs to the glucose-6-phosphate dehydrogenase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 513513Glucose-6-phosphate 1-dehydrogenase 2 HAMAP-Rule MF_00966
PRO_0000068086

Regions

Nucleotide binding38 – 458NADP 1 By similarity
Nucleotide binding401 – 4033NADP 2 By similarity
Nucleotide binding421 – 4233NADP 2 By similarity
Region201 – 2055Substrate binding By similarity

Sites

Active site2631Proton acceptor By similarity
Binding site721NADP 1 By similarity
Binding site1471NADP 1 By similarity
Binding site1711NADP 1; via carbonyl oxygen By similarity
Binding site1711Substrate By similarity
Binding site2391Substrate By similarity
Binding site2581Substrate By similarity
Binding site3571NADP 2 By similarity
Binding site3601Substrate By similarity
Binding site3651Substrate By similarity
Binding site3661NADP 2 By similarity
Binding site3701NADP 2 By similarity
Binding site3931NADP 2 By similarity
Binding site3951Substrate By similarity
Binding site4871NADP 2 By similarity
Binding site5031NADP 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P97324 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 655830EB767B6C53

FASTA51359,126
        10         20         30         40         50         60 
MAEQVTLSRT QVCGILREEL YQNDAFHQAD THIFIIMGAS GDLAKKKIYP TIWWLFRDGL 

        70         80         90        100        110        120 
LPKETFIVGY ARSQLTVDDI QKQSEPFFKA TPEERPKLEE FFTRNSYVVG QYDDPASYKH 

       130        140        150        160        170        180 
LNSYINALHQ GMQANHLFYL ALPPTVYEAV TKNIQETCMS QTGFNRIIVE KPFGRDLQSS 

       190        200        210        220        230        240 
NQLSNHISSL FREDQIYRID HYLDKEMVQN LMVLRFANRI FGPIWNGDNI VCVILTFKEP 

       250        260        270        280        290        300 
FGTEGRGGYF DEFGIIRDVM QSHLLQMLCL VAMEKPATTD SDDVRNEKVK VLKCISEVET 

       310        320        330        340        350        360 
DNVILGQYVG NPNGEGEAAN GYLDDPTVPR GSTTATFAAA VLYVKNERWD GVPFILRCGK 

       370        380        390        400        410        420 
ALNERKAEVR LQFRDIPGDI FHQKCKRNEL VIRMQPNEAV YTTMMTKKPG MFFNPEESEL 

       430        440        450        460        470        480 
DLTYGNKYKN VKLPGAYERL ILDVFCGCQM HFVRTDELRE GWRIFTPLLH KIEREKPQPF 

       490        500        510 
PYVYGSRGPT EADELMRRVG FQYKGTYKGT HKH 

« Hide

References

« Hide 'large scale' references
[1]"Testis-specific expression of a functional retroposon encoding glucose-6-phosphate dehydrogenase in the mouse."
Hendriksen P.J.M., Hoogerbrugge J.W., Baarends W.M., de Boer P., Vreeburg J.T.M., Vos E.A., van der Lende T., Grootegoed A.J.
Genomics 41:350-359(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, SUBUNIT, TISSUE SPECIFICITY.
Strain: Swiss.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z84471 Genomic DNA. Translation: CAB06476.1.
BC120827 mRNA. Translation: AAI20828.1.
BC137684 mRNA. Translation: AAI37685.1.
RefSeqNP_062341.2. NM_019468.2.
UniGeneMm.347430.

3D structure databases

ProteinModelPortalP97324.
SMRP97324. Positions 28-505.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000113269.

PTM databases

PhosphoSiteP97324.

Proteomic databases

PaxDbP97324.
PRIDEP97324.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID14380.
KEGGmmu:14380.

Organism-specific databases

CTD14380.
MGIMGI:105977. G6pd2.

Phylogenomic databases

eggNOGCOG0364.
HOGENOMHOG000046192.
HOVERGENHBG000856.
InParanoidQ0VB18.
KOK00036.

Enzyme and pathway databases

UniPathwayUPA00115; UER00408.

Gene expression databases

CleanExMM_G6PD2.
GenevestigatorP97324.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00966. G6PD.
InterProIPR001282. G6P_DH.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23429. PTHR23429. 1 hit.
PfamPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFPIRSF000110. G6PD. 1 hit.
PRINTSPR00079. G6PDHDRGNASE.
TIGRFAMsTIGR00871. zwf. 1 hit.
ProtoNetSearch...

Other

NextBio285889.
PROP97324.
SOURCESearch...

Entry information

Entry nameG6PD2_MOUSE
AccessionPrimary (citable) accession number: P97324
Secondary accession number(s): Q0VB18
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: February 19, 2014
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot