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P97321

- SEPR_MOUSE

UniProt

P97321 - SEPR_MOUSE

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Protein
Seprase
Gene
Fap
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM By similarity. May have a role in tissue remodeling during development and wound healing, and contribute to invasiveness in malignant cancers.

Catalytic activityi

Degrades gelatin and heat-denatured type I and type IV collagen, but not native type I or type IV collagen. Does not cleave laminin, fibronectin, fibrin or casein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei203 – 2031Substrate By similarity
Binding sitei204 – 2041Substrate By similarity
Active sitei624 – 6241Charge relay system By similarity
Active sitei702 – 7021Charge relay system By similarity
Active sitei734 – 7341Charge relay system By similarity

GO - Molecular functioni

  1. peptidase activity Source: MGI
  2. serine-type endopeptidase activity Source: InterPro
  3. serine-type peptidase activity Source: UniProtKB

GO - Biological processi

  1. endothelial cell migration Source: UniProtKB
  2. negative regulation of extracellular matrix disassembly Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS09.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Seprase (EC:3.4.21.-)
Alternative name(s):
Fibroblast activation protein alpha
Integral membrane serine protease
Gene namesi
Name:Fap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 2

Organism-specific databases

MGIiMGI:109608. Fap.

Subcellular locationi

Cell membrane; Single-pass type II membrane protein By similarity. Cell projectionlamellipodium membrane; Single-pass type II membrane protein By similarity. Cell projectioninvadopodium membrane; Single-pass type II membrane protein By similarity
Note: Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 44Cytoplasmic Reviewed prediction
Transmembranei5 – 2521Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini26 – 761736Extracellular Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical part of cell Source: MGI
  2. basal part of cell Source: MGI
  3. cell junction Source: UniProtKB-KW
  4. extracellular space Source: Ensembl
  5. integral component of membrane Source: UniProtKB-KW
  6. invadopodium membrane Source: UniProtKB-SubCell
  7. lamellipodium Source: UniProtKB
  8. lamellipodium membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 761761Seprase
PRO_0000122425Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi49 – 491N-linked (GlcNAc...) Reviewed prediction
Glycosylationi92 – 921N-linked (GlcNAc...) Reviewed prediction
Glycosylationi99 – 991N-linked (GlcNAc...) Reviewed prediction
Glycosylationi227 – 2271N-linked (GlcNAc...) Reviewed prediction
Glycosylationi314 – 3141N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi321 ↔ 332 By similarity
Disulfide bondi438 ↔ 441 By similarity
Disulfide bondi448 ↔ 466 By similarity
Disulfide bondi643 ↔ 756 By similarity
Glycosylationi679 – 6791N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP97321.
PRIDEiP97321.

PTM databases

PhosphoSiteiP97321.

Expressioni

Tissue specificityi

Detected in fibroblasts, in placenta, uterus, embryos from day 7-19 and in newborn mice (P1).

Gene expression databases

ArrayExpressiP97321.
BgeeiP97321.
CleanExiMM_FAP.
GenevestigatoriP97321.

Interactioni

Subunit structurei

Homodimer, or heterodimer with DPP4. The monomer is inactive By similarity.

Protein-protein interaction databases

MINTiMINT-4997001.

Structurei

3D structure databases

ProteinModelPortaliP97321.
SMRiP97321. Positions 40-758.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S9B family.

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1506.
GeneTreeiENSGT00740000115496.
HOGENOMiHOG000231875.
HOVERGENiHBG005527.
InParanoidiP97321.
KOiK08674.
OMAiQYYTARF.
OrthoDBiEOG761BT2.
PhylomeDBiP97321.
TreeFamiTF313309.

Family and domain databases

Gene3Di2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamiPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97321-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MKTWLKTVFG VTTLAALALV VICIVLRPSR VYKPEGNTKR ALTLKDILNG    50
TFSYKTYFPN WISEQEYLHQ SEDDNIVFYN IETRESYIIL SNSTMKSVNA 100
TDYGLSPDRQ FVYLESDYSK LWRYSYTATY YIYDLQNGEF VRGYELPRPI 150
QYLCWSPVGS KLAYVYQNNI YLKQRPGDPP FQITYTGREN RIFNGIPDWV 200
YEEEMLATKY ALWWSPDGKF LAYVEFNDSD IPIIAYSYYG DGQYPRTINI 250
PYPKAGAKNP VVRVFIVDTT YPHHVGPMEV PVPEMIASSD YYFSWLTWVS 300
SERVCLQWLK RVQNVSVLSI CDFREDWHAW ECPKNQEHVE ESRTGWAGGF 350
FVSTPAFSQD ATSYYKIFSD KDGYKHIHYI KDTVENAIQI TSGKWEAIYI 400
FRVTQDSLFY SSNEFEGYPG RRNIYRISIG NSPPSKKCVT CHLRKERCQY 450
YTASFSYKAK YYALVCYGPG LPISTLHDGR TDQEIQVLEE NKELENSLRN 500
IQLPKVEIKK LKDGGLTFWY KMILPPQFDR SKKYPLLIQV YGGPCSQSVK 550
SVFAVNWITY LASKEGIVIA LVDGRGTAFQ GDKFLHAVYR KLGVYEVEDQ 600
LTAVRKFIEM GFIDEERIAI WGWSYGGYVS SLALASGTGL FKCGIAVAPV 650
SSWEYYASIY SERFMGLPTK DDNLEHYKNS TVMARAEYFR NVDYLLIHGT 700
ADDNVHFQNS AQIAKALVNA QVDFQAMWYS DQNHGISSGR SQNHLYTHMT 750
HFLKQCFSLS D 761
Length:761
Mass (Da):87,945
Last modified:May 1, 1997 - v1
Checksum:i9174C3AEDA213B25
GO
Isoform 2 (identifier: P97321-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-35: Missing.

Show »
Length:756
Mass (Da):87,328
Checksum:iBE2A232BCEFD0C64
GO
Isoform 3 (identifier: P97321-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     31-63: Missing.

Show »
Length:728
Mass (Da):84,096
Checksum:i4436F2EC1C4B1BDA
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei31 – 6333Missing in isoform 3.
VSP_005369Add
BLAST
Alternative sequencei31 – 355Missing in isoform 2.
VSP_005368

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti737 – 7371S → L in AAH19190. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10007 mRNA. Translation: CAA71116.1.
BC019190 mRNA. Translation: AAH19190.1.
CCDSiCCDS16067.1. [P97321-1]
RefSeqiNP_032012.1. NM_007986.3. [P97321-1]
XP_006498809.1. XM_006498746.1. [P97321-1]
UniGeneiMm.41816.

Genome annotation databases

EnsembliENSMUST00000000402; ENSMUSP00000000402; ENSMUSG00000000392. [P97321-3]
ENSMUST00000102732; ENSMUSP00000099793; ENSMUSG00000000392. [P97321-1]
ENSMUST00000174448; ENSMUSP00000134386; ENSMUSG00000000392. [P97321-2]
GeneIDi14089.
KEGGimmu:14089.
UCSCiuc008jvk.1. mouse. [P97321-1]
uc008jvl.1. mouse. [P97321-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y10007 mRNA. Translation: CAA71116.1 .
BC019190 mRNA. Translation: AAH19190.1 .
CCDSi CCDS16067.1. [P97321-1 ]
RefSeqi NP_032012.1. NM_007986.3. [P97321-1 ]
XP_006498809.1. XM_006498746.1. [P97321-1 ]
UniGenei Mm.41816.

3D structure databases

ProteinModelPortali P97321.
SMRi P97321. Positions 40-758.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-4997001.

Chemistry

ChEMBLi CHEMBL5769.

Protein family/group databases

MEROPSi S09.007.

PTM databases

PhosphoSitei P97321.

Proteomic databases

PaxDbi P97321.
PRIDEi P97321.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000402 ; ENSMUSP00000000402 ; ENSMUSG00000000392 . [P97321-3 ]
ENSMUST00000102732 ; ENSMUSP00000099793 ; ENSMUSG00000000392 . [P97321-1 ]
ENSMUST00000174448 ; ENSMUSP00000134386 ; ENSMUSG00000000392 . [P97321-2 ]
GeneIDi 14089.
KEGGi mmu:14089.
UCSCi uc008jvk.1. mouse. [P97321-1 ]
uc008jvl.1. mouse. [P97321-3 ]

Organism-specific databases

CTDi 2191.
MGIi MGI:109608. Fap.

Phylogenomic databases

eggNOGi COG1506.
GeneTreei ENSGT00740000115496.
HOGENOMi HOG000231875.
HOVERGENi HBG005527.
InParanoidi P97321.
KOi K08674.
OMAi QYYTARF.
OrthoDBi EOG761BT2.
PhylomeDBi P97321.
TreeFami TF313309.

Miscellaneous databases

NextBioi 285126.
PROi P97321.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97321.
Bgeei P97321.
CleanExi MM_FAP.
Genevestigatori P97321.

Family and domain databases

Gene3Di 2.140.10.30. 1 hit.
3.40.50.1820. 1 hit.
InterProi IPR029058. AB_hydrolase.
IPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view ]
Pfami PF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view ]
SUPFAMi SSF53474. SSF53474. 1 hit.
PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse fibroblast activation protein: molecular cloning, alternative splicing and expression in the reactive stroma of epithelial cancers."
    Niedermeyer J., Scanlan M.J., Garin-Chesa P., Daiber C., Fiebig H.H., Old L.J., Rettig W.J., Schnapp A.
    Int. J. Cancer 71:383-389(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
    Strain: BALB/c.
    Tissue: Embryo.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Mammary gland.

Entry informationi

Entry nameiSEPR_MOUSE
AccessioniPrimary (citable) accession number: P97321
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi