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P97321 (SEPR_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Seprase

EC=3.4.21.-
Alternative name(s):
Fibroblast activation protein alpha
Integral membrane serine protease
Gene names
Name:Fap
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length761 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM By similarity. May have a role in tissue remodeling during development and wound healing, and contribute to invasiveness in malignant cancers.

Catalytic activity

Degrades gelatin and heat-denatured type I and type IV collagen, but not native type I or type IV collagen. Does not cleave laminin, fibronectin, fibrin or casein.

Subunit structure

Homodimer, or heterodimer with DPP4. The monomer is inactive By similarity.

Subcellular location

Cell membrane; Single-pass type II membrane protein By similarity. Cell projectionlamellipodium membrane; Single-pass type II membrane protein By similarity. Cell projectioninvadopodium membrane; Single-pass type II membrane protein By similarity. Note: Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma By similarity.

Tissue specificity

Detected in fibroblasts, in placenta, uterus, embryos from day 7-19 and in newborn mice (P1).

Sequence similarities

Belongs to the peptidase S9B family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97321-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97321-2)

The sequence of this isoform differs from the canonical sequence as follows:
     31-35: Missing.
Isoform 3 (identifier: P97321-3)

The sequence of this isoform differs from the canonical sequence as follows:
     31-63: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 761761Seprase
PRO_0000122425

Regions

Topological domain1 – 44Cytoplasmic Potential
Transmembrane5 – 2521Helical; Signal-anchor for type II membrane protein; Potential
Topological domain26 – 761736Extracellular Potential

Sites

Active site6241Charge relay system By similarity
Active site7021Charge relay system By similarity
Active site7341Charge relay system By similarity
Binding site2031Substrate By similarity
Binding site2041Substrate By similarity

Amino acid modifications

Glycosylation491N-linked (GlcNAc...) Potential
Glycosylation921N-linked (GlcNAc...) Potential
Glycosylation991N-linked (GlcNAc...) Potential
Glycosylation2271N-linked (GlcNAc...) Potential
Glycosylation3141N-linked (GlcNAc...) Potential
Glycosylation6791N-linked (GlcNAc...) Potential
Disulfide bond321 ↔ 332 By similarity
Disulfide bond438 ↔ 441 By similarity
Disulfide bond448 ↔ 466 By similarity
Disulfide bond643 ↔ 756 By similarity

Natural variations

Alternative sequence31 – 6333Missing in isoform 3.
VSP_005369
Alternative sequence31 – 355Missing in isoform 2.
VSP_005368

Experimental info

Sequence conflict7371S → L in AAH19190. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 9174C3AEDA213B25

FASTA76187,945
        10         20         30         40         50         60 
MKTWLKTVFG VTTLAALALV VICIVLRPSR VYKPEGNTKR ALTLKDILNG TFSYKTYFPN 

        70         80         90        100        110        120 
WISEQEYLHQ SEDDNIVFYN IETRESYIIL SNSTMKSVNA TDYGLSPDRQ FVYLESDYSK 

       130        140        150        160        170        180 
LWRYSYTATY YIYDLQNGEF VRGYELPRPI QYLCWSPVGS KLAYVYQNNI YLKQRPGDPP 

       190        200        210        220        230        240 
FQITYTGREN RIFNGIPDWV YEEEMLATKY ALWWSPDGKF LAYVEFNDSD IPIIAYSYYG 

       250        260        270        280        290        300 
DGQYPRTINI PYPKAGAKNP VVRVFIVDTT YPHHVGPMEV PVPEMIASSD YYFSWLTWVS 

       310        320        330        340        350        360 
SERVCLQWLK RVQNVSVLSI CDFREDWHAW ECPKNQEHVE ESRTGWAGGF FVSTPAFSQD 

       370        380        390        400        410        420 
ATSYYKIFSD KDGYKHIHYI KDTVENAIQI TSGKWEAIYI FRVTQDSLFY SSNEFEGYPG 

       430        440        450        460        470        480 
RRNIYRISIG NSPPSKKCVT CHLRKERCQY YTASFSYKAK YYALVCYGPG LPISTLHDGR 

       490        500        510        520        530        540 
TDQEIQVLEE NKELENSLRN IQLPKVEIKK LKDGGLTFWY KMILPPQFDR SKKYPLLIQV 

       550        560        570        580        590        600 
YGGPCSQSVK SVFAVNWITY LASKEGIVIA LVDGRGTAFQ GDKFLHAVYR KLGVYEVEDQ 

       610        620        630        640        650        660 
LTAVRKFIEM GFIDEERIAI WGWSYGGYVS SLALASGTGL FKCGIAVAPV SSWEYYASIY 

       670        680        690        700        710        720 
SERFMGLPTK DDNLEHYKNS TVMARAEYFR NVDYLLIHGT ADDNVHFQNS AQIAKALVNA 

       730        740        750        760 
QVDFQAMWYS DQNHGISSGR SQNHLYTHMT HFLKQCFSLS D 

« Hide

Isoform 2 [UniParc].

Checksum: BE2A232BCEFD0C64
Show »

FASTA75687,328
Isoform 3 [UniParc].

Checksum: 4436F2EC1C4B1BDA
Show »

FASTA72884,096

References

« Hide 'large scale' references
[1]"Mouse fibroblast activation protein: molecular cloning, alternative splicing and expression in the reactive stroma of epithelial cancers."
Niedermeyer J., Scanlan M.J., Garin-Chesa P., Daiber C., Fiebig H.H., Old L.J., Rettig W.J., Schnapp A.
Int. J. Cancer 71:383-389(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
Strain: BALB/c.
Tissue: Embryo.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y10007 mRNA. Translation: CAA71116.1.
BC019190 mRNA. Translation: AAH19190.1.
RefSeqNP_032012.1. NM_007986.3.
XP_006498809.1. XM_006498746.1.
UniGeneMm.41816.

3D structure databases

ProteinModelPortalP97321.
SMRP97321. Positions 40-758.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4997001.

Chemistry

ChEMBLCHEMBL5769.

Protein family/group databases

MEROPSS09.007.

PTM databases

PhosphoSiteP97321.

Proteomic databases

PaxDbP97321.
PRIDEP97321.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000402; ENSMUSP00000000402; ENSMUSG00000000392. [P97321-3]
ENSMUST00000102732; ENSMUSP00000099793; ENSMUSG00000000392. [P97321-1]
ENSMUST00000174448; ENSMUSP00000134386; ENSMUSG00000000392. [P97321-2]
GeneID14089.
KEGGmmu:14089.
UCSCuc008jvk.1. mouse. [P97321-1]
uc008jvl.1. mouse. [P97321-3]

Organism-specific databases

CTD2191.
MGIMGI:109608. Fap.

Phylogenomic databases

eggNOGCOG1506.
GeneTreeENSGT00740000115496.
HOGENOMHOG000231875.
HOVERGENHBG005527.
InParanoidP97321.
KOK08674.
OMARRNELPR.
OrthoDBEOG761BT2.
PhylomeDBP97321.
TreeFamTF313309.

Gene expression databases

ArrayExpressP97321.
BgeeP97321.
CleanExMM_FAP.
GenevestigatorP97321.

Family and domain databases

Gene3D2.140.10.30. 1 hit.
InterProIPR002471. Pept_S9_AS.
IPR001375. Peptidase_S9.
IPR002469. Peptidase_S9B.
[Graphical view]
PfamPF00930. DPPIV_N. 1 hit.
PF00326. Peptidase_S9. 1 hit.
[Graphical view]
PROSITEPS00708. PRO_ENDOPEP_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio285126.
PROP97321.
SOURCESearch...

Entry information

Entry nameSEPR_MOUSE
AccessionPrimary (citable) accession number: P97321
Entry history
Integrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: May 1, 1997
Last modified: April 16, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot