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P97321

- SEPR_MOUSE

UniProt

P97321 - SEPR_MOUSE

Protein

Seprase

Gene

Fap

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 125 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    In association with DPP4 is involved in the pericellular proteolysis of the extracellular matrix (ECM), the migration and invasion of endothelial cells into the ECM By similarity. May have a role in tissue remodeling during development and wound healing, and contribute to invasiveness in malignant cancers.By similarity

    Catalytic activityi

    Degrades gelatin and heat-denatured type I and type IV collagen, but not native type I or type IV collagen. Does not cleave laminin, fibronectin, fibrin or casein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei203 – 2031SubstrateBy similarity
    Binding sitei204 – 2041SubstrateBy similarity
    Active sitei624 – 6241Charge relay systemPROSITE-ProRule annotation
    Active sitei702 – 7021Charge relay systemPROSITE-ProRule annotation
    Active sitei734 – 7341Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. peptidase activity Source: MGI
    2. serine-type endopeptidase activity Source: InterPro
    3. serine-type peptidase activity Source: UniProtKB

    GO - Biological processi

    1. endothelial cell migration Source: UniProtKB
    2. negative regulation of extracellular matrix disassembly Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS09.007.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Seprase (EC:3.4.21.-)
    Alternative name(s):
    Fibroblast activation protein alpha
    Integral membrane serine protease
    Gene namesi
    Name:Fap
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:109608. Fap.

    Subcellular locationi

    Cell membrane By similarity; Single-pass type II membrane protein By similarity. Cell projectionlamellipodium membrane By similarity; Single-pass type II membrane protein By similarity. Cell projectioninvadopodium membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Found in cell surface lamellipodia, invadopodia and on shed vesicles. Colocalized with DPP4 in invadopodia and lamellipodia of migratory activated endothelial cells in collagenous matrix. Colocalized with DPP4 on endothelial cells of capillary-like microvessels but not large vessels within invasive breast ductal carcinoma By similarity.By similarity

    GO - Cellular componenti

    1. apical part of cell Source: MGI
    2. basal part of cell Source: MGI
    3. cell junction Source: UniProtKB-KW
    4. extracellular space Source: Ensembl
    5. integral component of membrane Source: UniProtKB-KW
    6. invadopodium membrane Source: UniProtKB-SubCell
    7. lamellipodium Source: UniProtKB
    8. lamellipodium membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 761761SeprasePRO_0000122425Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi49 – 491N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi99 – 991N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi227 – 2271N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi321 ↔ 332By similarity
    Disulfide bondi438 ↔ 441By similarity
    Disulfide bondi448 ↔ 466By similarity
    Disulfide bondi643 ↔ 756By similarity
    Glycosylationi679 – 6791N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP97321.
    PRIDEiP97321.

    PTM databases

    PhosphoSiteiP97321.

    Expressioni

    Tissue specificityi

    Detected in fibroblasts, in placenta, uterus, embryos from day 7-19 and in newborn mice (P1).

    Gene expression databases

    ArrayExpressiP97321.
    BgeeiP97321.
    CleanExiMM_FAP.
    GenevestigatoriP97321.

    Interactioni

    Subunit structurei

    Homodimer, or heterodimer with DPP4. The monomer is inactive By similarity.By similarity

    Protein-protein interaction databases

    MINTiMINT-4997001.

    Structurei

    3D structure databases

    ProteinModelPortaliP97321.
    SMRiP97321. Positions 40-758.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 44CytoplasmicSequence Analysis
    Topological domaini26 – 761736ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei5 – 2521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S9B family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1506.
    GeneTreeiENSGT00740000115496.
    HOGENOMiHOG000231875.
    HOVERGENiHBG005527.
    InParanoidiP97321.
    KOiK08674.
    OMAiQYYTARF.
    OrthoDBiEOG761BT2.
    PhylomeDBiP97321.
    TreeFamiTF313309.

    Family and domain databases

    Gene3Di2.140.10.30. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR001375. Peptidase_S9.
    IPR002469. Peptidase_S9B.
    [Graphical view]
    PfamiPF00930. DPPIV_N. 1 hit.
    PF00326. Peptidase_S9. 1 hit.
    [Graphical view]
    SUPFAMiSSF53474. SSF53474. 1 hit.
    PROSITEiPS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view]

    Sequences (3)i

    Sequence statusi: Complete.

    This entry describes 3 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P97321-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MKTWLKTVFG VTTLAALALV VICIVLRPSR VYKPEGNTKR ALTLKDILNG    50
    TFSYKTYFPN WISEQEYLHQ SEDDNIVFYN IETRESYIIL SNSTMKSVNA 100
    TDYGLSPDRQ FVYLESDYSK LWRYSYTATY YIYDLQNGEF VRGYELPRPI 150
    QYLCWSPVGS KLAYVYQNNI YLKQRPGDPP FQITYTGREN RIFNGIPDWV 200
    YEEEMLATKY ALWWSPDGKF LAYVEFNDSD IPIIAYSYYG DGQYPRTINI 250
    PYPKAGAKNP VVRVFIVDTT YPHHVGPMEV PVPEMIASSD YYFSWLTWVS 300
    SERVCLQWLK RVQNVSVLSI CDFREDWHAW ECPKNQEHVE ESRTGWAGGF 350
    FVSTPAFSQD ATSYYKIFSD KDGYKHIHYI KDTVENAIQI TSGKWEAIYI 400
    FRVTQDSLFY SSNEFEGYPG RRNIYRISIG NSPPSKKCVT CHLRKERCQY 450
    YTASFSYKAK YYALVCYGPG LPISTLHDGR TDQEIQVLEE NKELENSLRN 500
    IQLPKVEIKK LKDGGLTFWY KMILPPQFDR SKKYPLLIQV YGGPCSQSVK 550
    SVFAVNWITY LASKEGIVIA LVDGRGTAFQ GDKFLHAVYR KLGVYEVEDQ 600
    LTAVRKFIEM GFIDEERIAI WGWSYGGYVS SLALASGTGL FKCGIAVAPV 650
    SSWEYYASIY SERFMGLPTK DDNLEHYKNS TVMARAEYFR NVDYLLIHGT 700
    ADDNVHFQNS AQIAKALVNA QVDFQAMWYS DQNHGISSGR SQNHLYTHMT 750
    HFLKQCFSLS D 761
    Length:761
    Mass (Da):87,945
    Last modified:May 1, 1997 - v1
    Checksum:i9174C3AEDA213B25
    GO
    Isoform 2 (identifier: P97321-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         31-35: Missing.

    Show »
    Length:756
    Mass (Da):87,328
    Checksum:iBE2A232BCEFD0C64
    GO
    Isoform 3 (identifier: P97321-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         31-63: Missing.

    Show »
    Length:728
    Mass (Da):84,096
    Checksum:i4436F2EC1C4B1BDA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti737 – 7371S → L in AAH19190. (PubMed:15489334)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei31 – 6333Missing in isoform 3. 1 PublicationVSP_005369Add
    BLAST
    Alternative sequencei31 – 355Missing in isoform 2. 1 PublicationVSP_005368

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y10007 mRNA. Translation: CAA71116.1.
    BC019190 mRNA. Translation: AAH19190.1.
    CCDSiCCDS16067.1. [P97321-1]
    RefSeqiNP_032012.1. NM_007986.3. [P97321-1]
    XP_006498809.1. XM_006498746.1. [P97321-1]
    UniGeneiMm.41816.

    Genome annotation databases

    EnsembliENSMUST00000000402; ENSMUSP00000000402; ENSMUSG00000000392. [P97321-3]
    ENSMUST00000102732; ENSMUSP00000099793; ENSMUSG00000000392. [P97321-1]
    ENSMUST00000174448; ENSMUSP00000134386; ENSMUSG00000000392. [P97321-2]
    GeneIDi14089.
    KEGGimmu:14089.
    UCSCiuc008jvk.1. mouse. [P97321-1]
    uc008jvl.1. mouse. [P97321-3]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y10007 mRNA. Translation: CAA71116.1 .
    BC019190 mRNA. Translation: AAH19190.1 .
    CCDSi CCDS16067.1. [P97321-1 ]
    RefSeqi NP_032012.1. NM_007986.3. [P97321-1 ]
    XP_006498809.1. XM_006498746.1. [P97321-1 ]
    UniGenei Mm.41816.

    3D structure databases

    ProteinModelPortali P97321.
    SMRi P97321. Positions 40-758.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-4997001.

    Chemistry

    ChEMBLi CHEMBL5769.

    Protein family/group databases

    MEROPSi S09.007.

    PTM databases

    PhosphoSitei P97321.

    Proteomic databases

    PaxDbi P97321.
    PRIDEi P97321.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000402 ; ENSMUSP00000000402 ; ENSMUSG00000000392 . [P97321-3 ]
    ENSMUST00000102732 ; ENSMUSP00000099793 ; ENSMUSG00000000392 . [P97321-1 ]
    ENSMUST00000174448 ; ENSMUSP00000134386 ; ENSMUSG00000000392 . [P97321-2 ]
    GeneIDi 14089.
    KEGGi mmu:14089.
    UCSCi uc008jvk.1. mouse. [P97321-1 ]
    uc008jvl.1. mouse. [P97321-3 ]

    Organism-specific databases

    CTDi 2191.
    MGIi MGI:109608. Fap.

    Phylogenomic databases

    eggNOGi COG1506.
    GeneTreei ENSGT00740000115496.
    HOGENOMi HOG000231875.
    HOVERGENi HBG005527.
    InParanoidi P97321.
    KOi K08674.
    OMAi QYYTARF.
    OrthoDBi EOG761BT2.
    PhylomeDBi P97321.
    TreeFami TF313309.

    Miscellaneous databases

    NextBioi 285126.
    PROi P97321.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97321.
    Bgeei P97321.
    CleanExi MM_FAP.
    Genevestigatori P97321.

    Family and domain databases

    Gene3Di 2.140.10.30. 1 hit.
    3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR002471. Pept_S9_AS.
    IPR001375. Peptidase_S9.
    IPR002469. Peptidase_S9B.
    [Graphical view ]
    Pfami PF00930. DPPIV_N. 1 hit.
    PF00326. Peptidase_S9. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53474. SSF53474. 1 hit.
    PROSITEi PS00708. PRO_ENDOPEP_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse fibroblast activation protein: molecular cloning, alternative splicing and expression in the reactive stroma of epithelial cancers."
      Niedermeyer J., Scanlan M.J., Garin-Chesa P., Daiber C., Fiebig H.H., Old L.J., Rettig W.J., Schnapp A.
      Int. J. Cancer 71:383-389(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
      Strain: BALB/c.
      Tissue: Embryo.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiSEPR_MOUSE
    AccessioniPrimary (citable) accession number: P97321
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 5, 2002
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 125 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3