Reviewed, UniProtKB/Swiss-Prot P97318 (DAB1_MOUSE)

Names and origin

Protein names

Recommended name:
    Disabled homolog 1

Gene names

Name:

Dab1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	588 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adapter molecule functioning in neural development. May regulate SIAH1 activity.
Subunit structure	Interacts with LRP1 By similarity. Associates with the SH2 domains of SRC, FYN and ABL. Interacts with DAB2IP and SIAH1.
Tissue specificity	Expressed mainly in brain.
Domain	The PID domain specifically binds to the Asn-Pro-Xaa-Tyr(P) motif found in many tyrosine-phosphorylated proteins.
Post-translational modification	Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons. Also phosphorylated on Ser-524 independently of reelin signaling.
Sequence similarities	Contains 1 PID domain.

Ontologies

Keywords
Biological process	Differentiation Neurogenesis
Coding sequence diversity	Alternative splicing
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	cell-cell adhesion involved in neuronal-glial interactions involved in cerebral cortex radial glia guided migration Inferred from mutant phenotype. Source: MGI cerebellum structural organization Inferred from mutant phenotype. Source: MGI negative regulation of cell adhesion Inferred from mutant phenotype. Source: MGI neuron migration Inferred from mutant phenotype. Source: MGI positive regulation of protein kinase activity Inferred from genetic interaction. Source: MGI protein kinase cascade Traceable author statement. Source: UniProtKB radial glia guided migration of Purkinje cell Inferred from mutant phenotype. Source: MGI small GTPase mediated signal transduction Inferred from mutant phenotype. Source: MGI
Cellular component	intracellular Traceable author statement. Source: UniProtKB
Molecular function	SH2 domain binding Traceable author statement. Source: UniProtKB phospholipid binding Traceable author statement. Source: UniProtKB
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Aplp1	Q03157	2	EBI-81680,EBI-399929
Aplp2	Q06335	1	EBI-81680,EBI-446708
App	P12023	1	EBI-81680,EBI-78814
Lrp1	Q91ZX7	1	EBI-81680,EBI-300955
Pcdh18	Q8VHR0	2	EBI-81680,EBI-399910
Siah1a	P61092	2	EBI-81680,EBI-446761

Alternative products

This entry describes 7 isoforms produced by alternative splicing. [Align] [Select]

Isoform DAB588 (identifier: P97318-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: No experimental confirmation available.

Isoform DAB197 (identifier: P97318-4)

The sequence of this isoform differs from the canonical sequence as follows:
     2-23: STETELQVAVKTSAKKDSRKKG → LC
     200-217: YIVFEAGHEPIRDPETEE → VISEPRQGFACSCEGSFD
     218-588: Missing.

Notes: No experimental confirmation available.

Isoform DAB204 (identifier: P97318-5)

The sequence of this isoform differs from the canonical sequence as follows:
     200-204: YIVFE → NLQKN
     205-588: Missing.

Notes: No experimental confirmation available.

Isoform DAB217 (identifier: P97318-6)

The sequence of this isoform differs from the canonical sequence as follows:
     200-217: YIVFEAGHEPIRDPETEE → VISEPRQGFACSCEGSFD
     218-588: Missing.

Isoform DAB271 (identifier: P97318-3)

The sequence of this isoform differs from the canonical sequence as follows:
     240-272: Missing.
     275-304: AVTQLELFGDMSTPPDITSPPTPATPGDAF → SLVQSPAAERAEAESRTGPAEPGSILRPLG
     305-588: Missing.

Isoform DAB553 (identifier: P97318-8)

The sequence of this isoform differs from the canonical sequence as follows:
     187-221: Missing.

Notes: No experimental confirmation available.

Isoform DAB555 (identifier: P97318-2)

The sequence of this isoform differs from the canonical sequence as follows:
     240-272: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 588

588

Disabled homolog 1

PRO_0000079769

Regions

Domain

36 – 189

154

PID

Amino acid modifications

Modified residue

198

1

Phosphotyrosine

Modified residue

220

1

Phosphotyrosine

Modified residue

524

1

Phosphoserine; by CDK5

Natural variations

Alternative sequence

2 – 23

22

STETE…SRKKG → LC in isoform DAB197.

VSP_026205

Alternative sequence

187 – 221

35

Missing in isoform DAB553.

VSP_026206

Alternative sequence

200 – 217

18

YIVFE…PETEE → VISEPRQGFACSCEGSFD in isoform DAB197 and isoform DAB217.

VSP_003841

Alternative sequence

200 – 204

5

YIVFE → NLQKN in isoform DAB204.

VSP_026208

Alternative sequence

205 – 588

384

Missing in isoform DAB204.

VSP_026209

Alternative sequence

218 – 588

371

Missing in isoform DAB197 and isoform DAB217.

VSP_003842

Alternative sequence

240 – 272

33

Missing in isoform DAB555 and isoform DAB271.

VSP_003843

Alternative sequence

275 – 304

30

AVTQL…PGDAF → SLVQSPAAERAEAESRTGPA EPGSILRPLG in isoform DAB271.

VSP_003844

Alternative sequence

305 – 588

284

Missing in isoform DAB271.

VSP_003845

Experimental info

Sequence conflict

29

1

A → R in BAB24163. Ref.2

Sequence conflict

239

1

P → PVS in CAM17685 and CAM26758. Ref.3

Sequence conflict

248

1

E → D in CAM17685 and CAM26758. Ref.3

Secondary structure

1

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588

Helix Strand Turn

Details...

Helix

28 – 35

8

Beta strand

40 – 51

12

Beta strand

53 – 55

3

Helix

58 – 76

19

Turn

77 – 79

3

Beta strand

83 – 90

8

Beta strand

93 – 98

6

Turn

99 – 101

3

Beta strand

104 – 108

5

Helix

110 – 112

3

Beta strand

113 – 118

6

Beta strand

125 – 132

8

Beta strand

137 – 145

9

Helix

148 – 166

19

Helix

170 – 173

4

Sequences

	Sequence		Length	Mass (Da)
	Isoform DAB588 [UniParc]. Last modified June 1, 1998. Version 2. Checksum: 08404220792B1DB4	FASTA	588	63,578
	10 20 30 40 50 60 MSTETELQVA VKTSAKKDSR KKGQDRSEAT LIKRFKGEGV RYKAKLIGID EVSAARGDKL 70 80 90 100 110 120 CQDSMMKLKG VVAGARSKGE HKQKIFLTIS FGGIKIFDEK TGALQHHHAV HEISYIAKDI 130 140 150 160 170 180 TDHRAFGYVC GKEGNHRFVA IKTAQAAEPV ILDLRDLFQL IYELKQREEL EKKAQKDKQC 190 200 210 220 230 240 EQAVYQTILE EDVEDPVYQY IVFEAGHEPI RDPETEENIY QVPTSQKKEG VYDVPKSQPN 250 260 270 280 290 300 SQPLEDFESR FAAATPNRNL SMDFDELLEA TKVSAVTQLE LFGDMSTPPD ITSPPTPATP 310 320 330 340 350 360 GDAFLPSSSQ TLPGSADVFG SMSFGTAAVP SGYVAMGAVL PSFWGQQPLV QQQIAMGAQP 370 380 390 400 410 420 PVAQVIPGAQ PIAWGQPGLF PATQQAWPTV AGQFPPAAFM PTQTVMPLAA AMFQGPLTPL 430 440 450 460 470 480 ATVPGTNDSA RSSPQSDKPR QKMGKESFKD FQMVQPPPVP SRKPDQPSLT CTSEAFSSYF 490 500 510 520 530 540 NKVGVAQDTD DCDDFDISQL NLTPVTSTTP STNSPPTPAP RQSSPSKSSA SHVSDPTADD 550 560 570 580 IFEEGFESPS KSEEQEAPDG SQASSTSDPF GEPSGEPSGD NISPQDGS
	Isoform DAB197 [UniParc]. Checksum: 6AF789B4EC7FF6E9		197	22,236
	10 20 30 40 50 60 MLCQDRSEAT LIKRFKGEGV RYKAKLIGID EVSAARGDKL CQDSMMKLKG VVAGARSKGE 70 80 90 100 110 120 HKQKIFLTIS FGGIKIFDEK TGALQHHHAV HEISYIAKDI TDHRAFGYVC GKEGNHRFVA 130 140 150 160 170 180 IKTAQAAEPV ILDLRDLFQL IYELKQREEL EKKAQKDKQC EQAVYQTILE EDVEDPVYQV 190 ISEPRQGFAC SCEGSFD
	Isoform DAB204 [UniParc]. Checksum: 36271043EFC36BB4		204	23,077
	10 20 30 40 50 60 MSTETELQVA VKTSAKKDSR KKGQDRSEAT LIKRFKGEGV RYKAKLIGID EVSAARGDKL 70 80 90 100 110 120 CQDSMMKLKG VVAGARSKGE HKQKIFLTIS FGGIKIFDEK TGALQHHHAV HEISYIAKDI 130 140 150 160 170 180 TDHRAFGYVC GKEGNHRFVA IKTAQAAEPV ILDLRDLFQL IYELKQREEL EKKAQKDKQC 190 200 EQAVYQTILE EDVEDPVYQN LQKN
	Isoform DAB217 [UniParc]. Checksum: 17E7E398F5D1DC01		217	24,394
	10 20 30 40 50 60 MSTETELQVA VKTSAKKDSR KKGQDRSEAT LIKRFKGEGV RYKAKLIGID EVSAARGDKL 70 80 90 100 110 120 CQDSMMKLKG VVAGARSKGE HKQKIFLTIS FGGIKIFDEK TGALQHHHAV HEISYIAKDI 130 140 150 160 170 180 TDHRAFGYVC GKEGNHRFVA IKTAQAAEPV ILDLRDLFQL IYELKQREEL EKKAQKDKQC 190 200 210 EQAVYQTILE EDVEDPVYQV ISEPRQGFAC SCEGSFD
	Isoform DAB271 [UniParc]. Checksum: FA4A8CE9812EB55C		271	30,296
	10 20 30 40 50 60 MSTETELQVA VKTSAKKDSR KKGQDRSEAT LIKRFKGEGV RYKAKLIGID EVSAARGDKL 70 80 90 100 110 120 CQDSMMKLKG VVAGARSKGE HKQKIFLTIS FGGIKIFDEK TGALQHHHAV HEISYIAKDI 130 140 150 160 170 180 TDHRAFGYVC GKEGNHRFVA IKTAQAAEPV ILDLRDLFQL IYELKQREEL EKKAQKDKQC 190 200 210 220 230 240 EQAVYQTILE EDVEDPVYQY IVFEAGHEPI RDPETEENIY QVPTSQKKEG VYDVPKSQPV 250 260 270 SSLVQSPAAE RAEAESRTGP AEPGSILRPL G
	Isoform DAB553 [UniParc]. Checksum: 8B281EE7C3CFD2F2		553	59,415
	10 20 30 40 50 60 MSTETELQVA VKTSAKKDSR KKGQDRSEAT LIKRFKGEGV RYKAKLIGID EVSAARGDKL 70 80 90 100 110 120 CQDSMMKLKG VVAGARSKGE HKQKIFLTIS FGGIKIFDEK TGALQHHHAV HEISYIAKDI 130 140 150 160 170 180 TDHRAFGYVC GKEGNHRFVA IKTAQAAEPV ILDLRDLFQL IYELKQREEL EKKAQKDKQC 190 200 210 220 230 240 EQAVYQVPTS QKKEGVYDVP KSQPNSQPLE DFESRFAAAT PNRNLSMDFD ELLEATKVSA 250 260 270 280 290 300 VTQLELFGDM STPPDITSPP TPATPGDAFL PSSSQTLPGS ADVFGSMSFG TAAVPSGYVA 310 320 330 340 350 360 MGAVLPSFWG QQPLVQQQIA MGAQPPVAQV IPGAQPIAWG QPGLFPATQQ AWPTVAGQFP 370 380 390 400 410 420 PAAFMPTQTV MPLAAAMFQG PLTPLATVPG TNDSARSSPQ SDKPRQKMGK ESFKDFQMVQ 430 440 450 460 470 480 PPPVPSRKPD QPSLTCTSEA FSSYFNKVGV AQDTDDCDDF DISQLNLTPV TSTTPSTNSP 490 500 510 520 530 540 PTPAPRQSSP SKSSASHVSD PTADDIFEEG FESPSKSEEQ EAPDGSQASS TSDPFGEPSG 550 EPSGDNISPQ DGS
	Isoform DAB555 [UniParc]. Checksum: 57A7130311954FE0		555	59,838
	10 20 30 40 50 60 MSTETELQVA VKTSAKKDSR KKGQDRSEAT LIKRFKGEGV RYKAKLIGID EVSAARGDKL 70 80 90 100 110 120 CQDSMMKLKG VVAGARSKGE HKQKIFLTIS FGGIKIFDEK TGALQHHHAV HEISYIAKDI 130 140 150 160 170 180 TDHRAFGYVC GKEGNHRFVA IKTAQAAEPV ILDLRDLFQL IYELKQREEL EKKAQKDKQC 190 200 210 220 230 240 EQAVYQTILE EDVEDPVYQY IVFEAGHEPI RDPETEENIY QVPTSQKKEG VYDVPKSQPV 250 260 270 280 290 300 SAVTQLELFG DMSTPPDITS PPTPATPGDA FLPSSSQTLP GSADVFGSMS FGTAAVPSGY 310 320 330 340 350 360 VAMGAVLPSF WGQQPLVQQQ IAMGAQPPVA QVIPGAQPIA WGQPGLFPAT QQAWPTVAGQ 370 380 390 400 410 420 FPPAAFMPTQ TVMPLAAAMF QGPLTPLATV PGTNDSARSS PQSDKPRQKM GKESFKDFQM 430 440 450 460 470 480 VQPPPVPSRK PDQPSLTCTS EAFSSYFNKV GVAQDTDDCD DFDISQLNLT PVTSTTPSTN 490 500 510 520 530 540 SPPTPAPRQS SPSKSSASHV SDPTADDIFE EGFESPSKSE EQEAPDGSQA SSTSDPFGEP 550 SGEPSGDNIS PQDGS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mouse disabled (mDab1): a Src binding protein implicated in neuronal development." Howell B.W., Gertler F.B., Cooper J.A. EMBO J. 16:121-132(1997) [PubMed: 9009273] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS DAB217; DAB271 AND DAB555), FUNCTION, SUBUNIT, TISSUE SPECIFICITY. Tissue: Embryonic brain.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM DAB197). Strain: C57BL/6J. Tissue: Testis.
[3]	The mouse genome sequencing consortium Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"Identification of reelin-induced sites of tyrosyl phosphorylation on disabled 1." Keshvara L., Benhayon D., Magdaleno S., Curran T. J. Biol. Chem. 276:16008-16014(2001) [PubMed: 11279201] [Abstract] Cited for: PHOSPHORYLATION AT TYR-198 AND TYR-220.
[5]	"Cyclin-dependent kinase 5 phosphorylates disabled 1 independently of reelin signaling." Keshvara L., Magdaleno S., Benhayon D., Curran T. J. Neurosci. 22:4869-4877(2002) [PubMed: 12077184] [Abstract] Cited for: PHOSPHORYLATION AT SER-524.
[6]	"Inhibition of ubiquitin ligase Siah-1A by disabled-1." Park T.-J., Hamanaka H., Ohshima T., Watanabe N., Mikoshiba K., Nukina N. Biochem. Biophys. Res. Commun. 302:671-678(2003) [PubMed: 12646221] [Abstract] Cited for: INTERACTION WITH SIAH1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y08380 mRNA. Translation: CAA69663.1. Y08379 mRNA. Translation: CAA69662.1. Y08381 mRNA. Translation: CAA69664.1. Y08383 Genomic DNA. No translation available. AK005640 mRNA. Translation: BAB24163.1. AL627134, AL645483 Genomic DNA. Translation: CAM26758.1. AL627134, AL645483, AL669938 Genomic DNA. Translation: CAM26759.1. AL627134, AL645483, AL669938 Genomic DNA. Translation: CAM26760.1. AL627134, AL645483, AL669938 Genomic DNA. Translation: CAM26762.1. AL627134, AL645483, AL669938 Genomic DNA. Translation: CAM26765.1. AL645483, AL627134 Genomic DNA. Translation: CAM17685.1. AL645483, AL627134, AL669938 Genomic DNA. Translation: