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P97313 (PRKDC_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 136. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA-dependent protein kinase catalytic subunit

Short name=DNA-PK catalytic subunit
Short name=DNA-PKcs
EC=2.7.11.1
Alternative name(s):
p460
Gene names
Name:Prkdc
Synonyms:Xrcc7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length4128 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Serine/threonine-protein kinase that acts as a molecular sensor for DNA damage. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX, thereby regulating DNA damage response mechanism. Phosphorylates DCLRE1C, C1D, c-Abl/ABL1, histone H1, HSPCA, c-jun/JUN, p53/TP53, PARP1, POU2F1, DHX9, SRF, XRCC1, XRCC4, XRCC5, XRCC6, WRN, MYC and RFA2. Can phosphorylate C1D not only in the presence of linear DNA but also in the presence of supercoiled DNA. Ability to phosphorylate p53/TP53 in the presence of supercoiled DNA is dependent on C1D By similarity. Ref.12

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation

Inhibited by wortmannin. Activity of the enzyme seems to be attenuated by autophosphorylation By similarity.

Subunit structure

DNA-PK is a heterotrimer of PRKDC and the Ku p70-p86 (XRCC6-XRCC5) dimer. Formation of this complex may be promoted by interaction with ILF3. Associates with the DNA-bound Ku heterodimer, but it can also bind to and be activated by free DNA. Interacts with DNA-PKcs-interacting protein (KIP) with the region upstream the kinase domain. PRKDC alone also interacts with and phosphorylates DCLRE1C, thereby activating the latent endonuclease activity of this protein. Interacts with C1D By similarity. Interacts with TTI1 and TELO2 By similarity. Interacts with CIB1 By similarity.

Subcellular location

Nucleus. Nucleusnucleolus By similarity.

Post-translational modification

Autophosphorylated on Ser-2053, Thr-2605, Thr-2634 and Thr-2643. Ser-2053 and Thr-2605 are DNA damage-inducible phosphorylation sites (inducible with ionizing radiation, IR) dephosphorylated by PPP5C. Autophosphorylation induces a conformational change that leads to remodeling of the DNA-PK complex, requisite for efficient end processing and DNA repair By similarity. Ref.11

S-nitrosylated by GAPDH.

Involvement in disease

Defects in Prkdc are the cause of severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections if not chronically treated with antibiotics. The lack of B- and T-cell immunity resembles severe combined immunodeficiency syndrome in human infants. Ref.1

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 FAT domain.

Contains 1 FATC domain.

Contains 3 HEAT repeats.

Contains 1 PI3K/PI4K domain.

Contains 3 TPR repeats.

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentNucleus
   Coding sequence diversityAlternative splicing
Polymorphism
   DiseaseDisease mutation
SCID
   DomainRepeat
TPR repeat
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Serine/threonine-protein kinase
Transferase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell lineage commitment

Inferred from mutant phenotype PubMed 8788039. Source: MGI

T cell differentiation in thymus

Inferred from mutant phenotype PubMed 9768755. Source: MGI

T cell lineage commitment

Inferred from mutant phenotype PubMed 8788039. Source: MGI

T cell receptor V(D)J recombination

Inferred from mutant phenotype PubMed 9768755. Source: MGI

brain development

Inferred from genetic interaction PubMed 16687486. Source: MGI

cellular response to insulin stimulus

Inferred from electronic annotation. Source: Ensembl

double-strand break repair

Inferred from mutant phenotype PubMed 11751629PubMed 12604618. Source: MGI

double-strand break repair via nonhomologous end joining

Inferred from electronic annotation. Source: InterPro

ectopic germ cell programmed cell death

Inferred from mutant phenotype PubMed 17145779. Source: MGI

heart development

Inferred from genetic interaction PubMed 16687486. Source: MGI

immunoglobulin V(D)J recombination

Inferred from mutant phenotype PubMed 9768755. Source: MGI

intrinsic apoptotic signaling pathway in response to DNA damage

Inferred from mutant phenotype PubMed 12604618. Source: MGI

lymphocyte differentiation

Inferred from mutant phenotype PubMed 8133891. Source: MGI

peptidyl-serine phosphorylation

Inferred from electronic annotation. Source: Ensembl

positive regulation of apoptotic process

Inferred from mutant phenotype PubMed 17145779. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from electronic annotation. Source: Ensembl

pro-B cell differentiation

Inferred from mutant phenotype PubMed 9768755. Source: MGI

protein destabilization

Inferred from direct assay PubMed 15194694. Source: MGI

response to gamma radiation

Inferred from mutant phenotype PubMed 9768755. Source: MGI

somitogenesis

Inferred from genetic interaction PubMed 16687486. Source: MGI

telomere maintenance

Inferred from mutant phenotype PubMed 15010310. Source: MGI

   Cellular_componentDNA-dependent protein kinase-DNA ligase 4 complex

Inferred from electronic annotation. Source: Ensembl

nonhomologous end joining complex

Inferred from sequence or structural similarity. Source: UniProtKB

nucleolus

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 12604618. Source: MGI

transcription factor complex

Inferred from electronic annotation. Source: Ensembl

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from electronic annotation. Source: InterPro

DNA-dependent protein kinase activity

Inferred from electronic annotation. Source: Ensembl

enzyme binding

Inferred from physical interaction Ref.13. Source: UniProtKB

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: A number of isoforms are produced.
Isoform 1 (identifier: P97313-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P97313-2)

The sequence of this isoform differs from the canonical sequence as follows:
     842-852: DEALSLEEIRI → VRNPFLILYLK
     853-4128: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 41284128DNA-dependent protein kinase catalytic subunit
PRO_0000225599

Regions

Repeat288 – 32336HEAT 1
Repeat1001 – 103737HEAT 2
Repeat1050 – 108637HEAT 3
Repeat1720 – 175334TPR 1
Domain2884 – 3539656FAT
Repeat2921 – 295434TPR 2
Repeat2956 – 298328TPR 3
Domain3747 – 4015269PI3K/PI4K
Domain4096 – 412833FATC
Region1501 – 153636Interaction with C1D By similarity
Region1501 – 153636Leucine-zipper
Region2432 – 3213782KIP-binding By similarity

Sites

Site2017 – 20182Cleavage; by caspase-3 By similarity

Amino acid modifications

Modified residue1171N6-acetyllysine By similarity
Modified residue8911Phosphoserine By similarity
Modified residue12061N6-acetyllysine By similarity
Modified residue19671N6-acetyllysine By similarity
Modified residue20531Phosphoserine; by autocatalysis By similarity
Modified residue22551N6-acetyllysine By similarity
Modified residue26051Phosphothreonine; by autocatalysis By similarity
Modified residue26081Phosphoserine; by autocatalysis By similarity
Modified residue26341Phosphothreonine; by autocatalysis By similarity
Modified residue26431Phosphothreonine; by autocatalysis By similarity
Modified residue32061Phosphoserine By similarity
Modified residue32411N6-acetyllysine By similarity
Modified residue32601N6-acetyllysine By similarity
Modified residue36381N6-acetyllysine By similarity
Modified residue36421N6-acetyllysine By similarity
Modified residue40261Phosphoserine By similarity

Natural variations

Alternative sequence842 – 85211DEALSLEEIRI → VRNPFLILYLK in isoform 2.
VSP_017361
Alternative sequence853 – 41283276Missing in isoform 2.
VSP_017362
Natural variant21401R → C. Ref.1 Ref.2 Ref.3
Natural variant31911L → P.
Natural variant4046 – 412883Missing in SCID.

Experimental info

Sequence conflict33001M → T in BAA19566. Ref.1
Sequence conflict33001M → T in BAA28873. Ref.2
Sequence conflict33001M → T in BAA28875. Ref.2
Sequence conflict33001M → T in BAB91149. Ref.3
Sequence conflict38441M → V in AAB36939. Ref.10
Sequence conflict38441M → V in AAB36940. Ref.10

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: B143922D57F4331E

FASTA4,128471,471
        10         20         30         40         50         60 
MAEEGTGVRC WLLQLQEFLS AADRCSAAGA SYQLIRSLGQ ECVLSTSSAV QALQISLVFS 

        70         80         90        100        110        120 
RDFGLLVFIR KSLSIEDFRD CREEALKFLC VFLEKIDQKV MHYSLDIKNT CTSVYTKDRT 

       130        140        150        160        170        180 
AKCKIPALDL LIKLLQILRS TRLMDEFKIG ELFNKFYGEL ASKSKLPDTV LEKVYELLGV 

       190        200        210        220        230        240 
LGEVHPSEMI NHSENLFRAF LGELKTQMTS TVREPKFPVL AGCLKGLSSL LCNFTKSMEE 

       250        260        270        280        290        300 
DPQTSKEIFG FTFKAIRPQI EMKRYAVPLA GLRLLTLHAS QFTACLLDNY ITLFEVLSKW 

       310        320        330        340        350        360 
CSHTNVELKK AAHSALESFL RQISFTVAED AELHKSRLKY FMEQFYGIIR NTDSNNKELA 

       370        380        390        400        410        420 
IAIRGYGLFA GPCKVINAKD VDFMYVELIQ RCKQMFLTHA DASEDHVYQM PSFLQSIASV 

       430        440        450        460        470        480 
LLYLDTVPEV YTPVLEHLMV VQIDSFPQYS PKMQLVCCKA IIKLFLALSE KGPVHWNCIS 

       490        500        510        520        530        540 
AVVHQGLIRI CSKPVVLQKD VESRSDNRSA SEEVRTGRWK VPTYKDYVDL FQHLLGCDQM 

       550        560        570        580        590        600 
EDFILGDETF LFVNSSLKSL NHLLYDEFIR SVLKIVEKLD LTLEKQTVGE QEDGSTADVW 

       610        620        630        640        650        660 
VIPTSDPAAN LHPAKPSDFS ALINLVEFCR EILPRKHVGF FEPWVYSFAY ELILQSTRLP 

       670        680        690        700        710        720 
LISGFYKLLS IAVKNARKIK YFEGISPKSL KHSPEDTEKY SCFALFAKFG KEVSVKMKQY 

       730        740        750        760        770        780 
KDELLASCLT FVLSLPHDII ELDVRAYVPA LQMAFKLGLS HMPLAEIGLH ALKEWSVHID 

       790        800        810        820        830        840 
KSILQPYYKD ILPCLDGYLN TSTLSDETKS HWGLSALSRA AQKGFNRHVV KHLKRTRNSS 

       850        860        870        880        890        900 
PDEALSLEEI RIKVVQILGS LGGQINKSLV TATSGERMKK YVAWDAERRL SFAVPFREMK 

       910        920        930        940        950        960 
PVIYLDVFLP RVTELALSAS DRQTKVAACE LLHSMVMFML GRATQMPEGQ GLPPMYQLYK 

       970        980        990       1000       1010       1020 
HTFPVLLQLA CDVDQVTRQL YEPLVMQLIH WLTNNKKFES QDTVALLEAI LDGIVDPVDS 

      1030       1040       1050       1060       1070       1080 
TLRDFCGRCV QEFLKWSIKQ TTPQQQEKSP VNSKSLFKRL YSLALHPNAF KRLGAALAFN 

      1090       1100       1110       1120       1130       1140 
HIYKEFREEG SLVEQFVFEA LVTYMESLAL AHEDEKSLGT VQQCCDAIDH LRRIIEKKHV 

      1150       1160       1170       1180       1190       1200 
SLNKAKKRRL PQGFPPLTSL CLLDLVEWLL AHCGRPQTEC RHKSMELFYK FVPLLPGNKS 

      1210       1220       1230       1240       1250       1260 
PSLWLKDLIK KKGISFLINT FEGGASSSDQ PAGILAQPTL VYLQGPISLR GVLQWLDLLL 

      1270       1280       1290       1300       1310       1320 
AALECYNTFI EKETVQGQEV LGAEVQSSLL KSVAFFLESI ATHSARAVEQ RFGSGAPGPP 

      1330       1340       1350       1360       1370       1380 
SLHEEEKYNY SKCTVLVRIM EFTTTLLIAS PEDCKLLEKD LCNTNLMQVL VKMICEPMSL 

      1390       1400       1410       1420       1430       1440 
GFNIGDVQVM NHLPSICVNL LKALRKSPYR DMLETHLKEK VTVQSVEELC SINLCSSGAR 

      1450       1460       1470       1480       1490       1500 
QERSKLLSIL SACKQLHKAG FSHVISPSQS TALNHSVGMR LLSLVYKGIV PAEERQCLQS 

      1510       1520       1530       1540       1550       1560 
LDPSCKSLAN GLLELAFGFG GLCDHLVSLL LNSAMLSTQY LGSSQRNISF SHGEYFYSLF 

      1570       1580       1590       1600       1610       1620 
SEVINSELLK NLDIAVSRLM ESSSDNPKMV STVLNGMLDT SFRDRAVQKH QGLKLATAIL 

      1630       1640       1650       1660       1670       1680 
QNWRKCDSWW APDSAPESKT TVLSLLAKML QIDSALSFDT NHSSFSEIFT TYASLLADTK 

      1690       1700       1710       1720       1730       1740 
LGLHLKGQAI ILLPFFTSLR EGSLENLKHI LEKLIVCNFP MKSDEFPPDS LKYNNYVDCM 

      1750       1760       1770       1780       1790       1800 
KKFLDALELS QSPMLFQLMT DILCREQRHI MEELFQTTFK RIARQSPCVT QLNLLESVYT 

      1810       1820       1830       1840       1850       1860 
MFRKADLPSN VTRQAFVDRS LLTLLWHCDL DTLKEFFSRI VVDAIDVLKS RFTKLNEFTF 

      1870       1880       1890       1900       1910       1920 
DTQITKKMCY YKMLAVMYSR LLKDDVHSKE AKINQAFHGS RVAEGNELTK TLLKLCHDAF 

      1930       1940       1950       1960       1970       1980 
TENMVGESQL LEKRRLYHCA AYNCAISLIS CVFNELKFYQ GFLFNEKPEK NLFIFENLID 

      1990       2000       2010       2020       2030       2040 
LKRCYTFPIE VEVPMERKKK YIEIRKEARD AANGASGSPH YMSSLSYLTD SSLSEEMSQF 

      2050       2060       2070       2080       2090       2100 
DFSTGVQSYS YSSQDRKPTT GHFQRREHQD SMTQDDIMEL EMDELNQHEC MAPMIALIKH 

      2110       2120       2130       2140       2150       2160 
MQRNVIAPKG EEGSIPKDLP PWMKFLHDKL GNASVSLNIR LFLAKLVINT EEVFRPYAKH 

      2170       2180       2190       2200       2210       2220 
WLSPLLQLAV CENNREGIHY MMVEIVATIL SWTGLATPTG VPKDEVLANR LLRFLMKHVF 

      2230       2240       2250       2260       2270       2280 
HPKRAVFRHN LEIIKTLVEC WKECLSIPYR LIFEKFSHKD PNSKDNSVGI QLLGIVIANN 

      2290       2300       2310       2320       2330       2340 
LPPYDPNCDI TSAMYFEALV NNMSFVKYKE VYAAAAEVLG LILQYITERK HVIAELVCEL 

      2350       2360       2370       2380       2390       2400 
VIKQLKQHQN TMEDKFIVCL NKIAKGFPPL ADRFLNALFF LLPKFHGVMK TLCLEVVLCR 

      2410       2420       2430       2440       2450       2460 
AEEITGLYLQ LKSKDFLQVM RHRDDERQKV CLDIVYKMVA KLKPIELREL LNPVVEFVSH 

      2470       2480       2490       2500       2510       2520 
PSPTCREQMY NILMWIHDNY RDQESQNDED SQEIFKLAKD VLIQGLIDEN VGLQLIIRNF 

      2530       2540       2550       2560       2570       2580 
WSHETRLPSN TLDRLLALNS LYSPKIEVHF LSLATNFLLE MTRMSPDYLN PIFEHPLSEC 

      2590       2600       2610       2620       2630       2640 
EFQEYTIDPD WRFRSTVLTP MFIETQASPS ILHTQTQEGP LSDQRQKPGQ VRATQQQYDF 

      2650       2660       2670       2680       2690       2700 
TPTQASVERS SFDWLTGSSI DLLADHTVFS SETLSSSLLF SHKRTEKSQR MSCKSVGPDF 

      2710       2720       2730       2740       2750       2760 
GTKKLGLPDD EVDNQVKSGT PSQADILRLR RRFLKDREKL SLLYAKRGLM EQKLEKDIKS 

      2770       2780       2790       2800       2810       2820 
EFKMKQDAQV VLYRSYRHGD LPDIQIQHSG LITPLQAVAQ KDPIIAKQLF SSLFSGILKE 

      2830       2840       2850       2860       2870       2880 
MNKFKTTSEK NIITQNLLQD FNRFLNTTFL FFPPFVSCIQ EISCQHPDFL TLDPASVRVG 

      2890       2900       2910       2920       2930       2940 
CLASLQQPGG IRLLEEALLR LMPKEPPTKR VRGKTCLPPD VLRWMELAKL YRSIGEYDVL 

      2950       2960       2970       2980       2990       3000 
RGIFSSELGT TQDTQNALLA EARSDYCQAA KLYDEALNKL EWVDGEPTEA EKEFWELASL 

      3010       3020       3030       3040       3050       3060 
DCYNNLSKWK ELEYCSTVNI VSENSLDLSK MWSEPFYQET YLPYVIRSKL KLLLQGEGNQ 

      3070       3080       3090       3100       3110       3120 
SLLTFVDEAM NKELQKTVLE LQYSQELSLL YILQDDIDRA TYYIKNGIQI FMQNYSSIDV 

      3130       3140       3150       3160       3170       3180 
LLYRSRLAKL QSVQTLAEIE EFLSFICKHG DLSSLGPLRR LLKTWTSRYP DVVTDPMHIW 

      3190       3200       3210       3220       3230       3240 
DDIITNRCFF LSKIEERLTA PSGDHSMSVD EDEESIDREV YEPKEDVRCM LQSCRFTMKM 

      3250       3260       3270       3280       3290       3300 
KMIESAWKQS NFSLSMKLLK EMHKESKTRE IWRVQWLHSY SQLNHCRSHT QSPREQVLNM 

      3310       3320       3330       3340       3350       3360 
LKTITLLDES DISNYLNKNI QASCDQSILL GTTCRIMADA LSREPACLSD LEENKVNSIL 

      3370       3380       3390       3400       3410       3420 
TLSGSNAENT ETVITGLYQR AFHHLSKAVQ SAEEETQLSC WGHEAAAERA HAYMTLVGFC 

      3430       3440       3450       3460       3470       3480 
DQQLRKVEES ASQKTSAEME AYPALVVEKM LRALKLNSSE ARLKFPRLLQ IIEQYSEETL 

      3490       3500       3510       3520       3530       3540 
NIMTKEISSI PCWQFIGWIS HMMALLDKEE AIAVQHTVEE IADNYPQAII YPFIISSESY 

      3550       3560       3570       3580       3590       3600 
SFKNTSSGHN NKAFVERIKS KLDHGEVIHS FINALDQLSN PDLLFKDWVS DTKDELGKNP 

      3610       3620       3630       3640       3650       3660 
VNKKNIEKLY ERMYAALGDL RAPGLGPFRR RFIQAFGKEF VKSFGNGGSK LLTMKVDDFC 

      3670       3680       3690       3700       3710       3720 
KITGSLLVRM KKDSKLPGNL KEYSPWMSEF KAQFLKNELE IPGQYDGKSK PLPEYHVRIS 

      3730       3740       3750       3760       3770       3780 
GFDERVKVML SLRKPKRIVI RGHDEKEYPF LVKGGEDLRQ DQRIEQIFEV MNAILSQDAA 

      3790       3800       3810       3820       3830       3840 
CSQRNMQLRT YRVVPMTSRL GLIEWIENTM TLKDLLLSNM SQEEKVANNS DPKAPIRDYK 

      3850       3860       3870       3880       3890       3900 
DWLMKVSGKS DAGAYVLMYS RANRTETVVA FRRRESQVPP DLLKRAFVKM STSPEAFLAL 

      3910       3920       3930       3940       3950       3960 
RSHFASSHAL LCISHWLLGI GDRHLNNFMV AMETGSVIGI DFGHAFGSAT QFLPVPELMP 

      3970       3980       3990       4000       4010       4020 
FRLTRQFVSL MLPMKETGLM CTVMVHALRA FRSCAGLLTD TMEIFVKEPS FDWKSFEQTM 

      4030       4040       4050       4060       4070       4080 
LRKGGSWIQE INVTEKNWYP QHKIRYAKRK LAGANPAVIT CDELYLGHEA SSAFRSYTAV 

      4090       4100       4110       4120 
ARGNRDYNIR AQEPESGLSE ETQVKCLVDQ ATDPNILGRT WEGWEPWM 

« Hide

Isoform 2 [UniParc].

Checksum: 813746121866A369
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FASTA85296,914

References

« Hide 'large scale' references
[1]"Nonsense mutation at Tyr-4046 in the DNA-dependent protein kinase catalytic subunit of severe combined immune deficiency mice."
Araki R., Fujimori A., Hamatani K., Mita K., Saito T., Mori M., Fukumura R., Morimyo M., Muto M., Itoh M., Tatsumi K., Abe M.
Proc. Natl. Acad. Sci. U.S.A. 94:2438-2443(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT CYS-2140, VARIANT SCID 4046-TYR--MET-4128 DEL.
Strain: C.B17.
Tissue: Fibroblast and Leukocyte.
[2]"Murine cell line SX9 bearing a mutation in the DNA-PKcs gene exhibits aberrant V(D)J recombination not only in the coding joint but also in the signal joint."
Fukumura R., Araki R., Fujimori A., Mori M., Saito T., Watanabe F., Sarashi M., Itsukaichi H., Eguch-Kasai K., Sato K., Tatsumi K., Abe M.
J. Biol. Chem. 273:13058-13064(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT CYS-2140.
[3]"The murine DNA-PKcs gene consists of 86 exons dispersed in more than 250 kb."
Fujimori A., Araki R., Fukumura R., Saito T., Mori M., Mita K., Tatsumi K., Abe M.
Genomics 45:194-199(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT CYS-2140.
Strain: 129/SvJ.
[4]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3618-4128.
Strain: C57BL/6J and NOD.
Tissue: Heart and Thymus.
[5]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[6]"Mouse Cdc21 only 0.5 kb upstream from DNA-PKcs in a head-to-head organization: an implication of co-evolution of ATM family members and cell cycle regulating genes."
Saito T., Matsuda Y., Ishii H., Watanabe F., Mori M., Hayashi A., Araki R., Fujimori A., Fukumura R., Morimyo M., Tatsumi K., Hori T., Abe M.
Mamm. Genome 9:769-772(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-26.
[7]"Nonhomologous end-joining deficiency of L5178Y-S cells is not associated with mutation in the ABCDE autophosphorylation cluster."
Brzoska K., Kruszewski M., Szumiel I.
Acta Biochim. Pol. 53:233-236(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 2550-2658.
[8]"Cloning and chromosomal mapping of the mouse DNA-dependent protein kinase gene."
Hamatani K., Matsuda Y., Araki R., Itoh M., Abe M.
Immunogenetics 45:1-5(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3615-4128.
Strain: BALB/c.
Tissue: Leukocyte.
[9]"Identification of a nonsense mutation in the carboxyl-terminal region of DNA-dependent protein kinase catalytic subunit in the scid mouse."
Blunt T., Gell D., Fox M., Taccioli G.E., Lehmann A.R., Jackson S.P., Jeggo P.A.
Proc. Natl. Acad. Sci. U.S.A. 93:10285-10290(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3680-4128.
[10]"Biochemical and genetic defects in the DNA-dependent protein kinase in murine scid lymphocytes."
Danska J.S., Holland D.P., Mariathasan S., Williams K.M., Guidos C.J.
Mol. Cell. Biol. 16:5507-5517(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 3839-4128.
Strain: C.B17.
[11]"Functional interaction between DNA-PK and c-Abl in response to DNA damage."
Kharbanda S., Pandey P., Jin S., Inoue S., Bharti A., Yuan Z.-M., Weichselbaum R., Weaver D., Kufe D.
Nature 386:732-735(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION OF ABL1.
[12]"Functional interaction between DNA-PKcs and telomerase in telomere length maintenance."
Espejel S., Franco S., Sgura A., Gae D., Bailey S.M., Taccioli G.E., Blasco M.A.
EMBO J. 21:6275-6287(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[13]"GAPDH mediates nitrosylation of nuclear proteins."
Kornberg M.D., Sen N., Hara M.R., Juluri K.R., Nguyen J.V., Snowman A.M., Law L., Hester L.D., Snyder S.H.
Nat. Cell Biol. 12:1094-1100(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: S-NITROSYLATION BY GAPDH.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D87521 mRNA. Translation: BAA19566.1.
AB007544 mRNA. Translation: BAA28873.1.
AB011543 mRNA. Translation: BAA28875.1.
AB030754 Genomic DNA. Translation: BAB91149.1.
AK084827 mRNA. Translation: BAE43387.1.
AK088981 mRNA. Translation: BAC40685.1.
AC111103 Genomic DNA. No translation available.
AC154586 Genomic DNA. No translation available.
CT010522 Genomic DNA. No translation available.
CT030649 Genomic DNA. No translation available.
AB000629 Genomic DNA. Translation: BAA34640.1.
DQ235257 mRNA. Translation: ABB36568.1.
DQ235258 mRNA. Translation: ABB36569.1.
D83786 mRNA. Translation: BAA12115.1.
U78157 mRNA. Translation: AAB36939.1.
U78158 mRNA. Translation: AAB36940.1.
PIRJC6306.
RefSeqNP_035289.2. NM_011159.2.
UniGeneMm.71.

3D structure databases

ProteinModelPortalP97313.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202371. 6 interactions.
IntActP97313. 4 interactions.
MINTMINT-4108568.
STRING10090.ENSMUSP00000023352.

Chemistry

ChEMBLCHEMBL2176779.

PTM databases

PhosphoSiteP97313.

Proteomic databases

PaxDbP97313.
PRIDEP97313.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000023352; ENSMUSP00000023352; ENSMUSG00000022672. [P97313-1]
GeneID19090.
KEGGmmu:19090.
UCSCuc007yhs.1. mouse. [P97313-2]
uc007yht.1. mouse. [P97313-1]

Organism-specific databases

CTD5591.
MGIMGI:104779. Prkdc.

Phylogenomic databases

eggNOGCOG5032.
GeneTreeENSGT00720000108767.
HOVERGENHBG053681.
InParanoidP97313.
KOK06642.
OMAYNYSKCT.
OrthoDBEOG7DNNT7.
TreeFamTF324494.

Gene expression databases

ArrayExpressP97313.
BgeeP97313.
GenevestigatorP97313.

Family and domain databases

Gene3D1.10.1070.11. 3 hits.
1.25.10.10. 1 hit.
InterProIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR003152. FATC.
IPR011009. Kinase-like_dom.
IPR012582. NUC194.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR003151. PIK-rel_kinase_FAT.
IPR014009. PIK_FAT.
[Graphical view]
PfamPF02259. FAT. 1 hit.
PF02260. FATC. 1 hit.
PF08163. NUC194. 1 hit.
PF00454. PI3_PI4_kinase. 1 hit.
[Graphical view]
SMARTSM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 9 hits.
SSF56112. SSF56112. 2 hits.
PROSITEPS51189. FAT. 1 hit.
PS51190. FATC. 1 hit.
PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295644.
PROP97313.
SOURCESearch...

Entry information

Entry namePRKDC_MOUSE
AccessionPrimary (citable) accession number: P97313
Secondary accession number(s): E9QN15 expand/collapse secondary AC list , O88187, P97928, Q307W9, Q3V2W8, Q8C2A7, Q9Z341
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 136 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot