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P97311 (MCM6_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA replication licensing factor MCM6

EC=3.6.4.12
Alternative name(s):
Mis5 homolog
Gene names
Name:Mcm6
Synonyms:Mcmd6, Mis5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length821 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). May interact with MCM10. Interacts with TIPIN. Interacts with MCMBP By similarity.

Subcellular location

Nucleus By similarity. Note: Binds to chromatin during G1 and detach from it during S phase By similarity.

Post-translational modification

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner By similarity.

Sequence similarities

Belongs to the MCM family.

Contains 1 MCM domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Cdt1Q8R4E92EBI-457132,EBI-457043

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 821821DNA replication licensing factor MCM6
PRO_0000194114

Regions

Domain346 – 553208MCM
Nucleotide binding396 – 4038ATP Potential
Motif528 – 5314Arginine finger

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue131Phosphoserine By similarity
Modified residue2711Phosphoserine By similarity
Modified residue6431N6-acetyllysine Ref.4
Modified residue7621Phosphoserine By similarity
Modified residue7911Phosphothreonine Ref.3

Sequences

Sequence LengthMass (Da)Tools
P97311 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 79E753D2DA218CBF

FASTA82192,867
        10         20         30         40         50         60 
MDLAAAAEPG AGSQHPEVRD EVAEKCQKLF LDFLEEFQGS DGEIKYLQFA EELIRPERNT 

        70         80         90        100        110        120 
LVVSFADLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD RKEIPFAKDF YVAFQDLPTR 

       130        140        150        160        170        180 
HKIRELTSSR IGLLTRISGQ VVRTHPVHPE LVSGTFLCLD CQTVIKDVEQ QFKYTQPNIC 

       190        200        210        220        230        240 
RNPVCANRKR FLLDTNKSRF VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD 

       250        260        270        280        290        300 
RCDFTGALIV VPDVSKLSTP GARAETNSRV SGADGYETEG IRGLRALGVR DLSYRLVFLA 

       310        320        330        340        350        360 
CHVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL CTSLFPTIHG 

       370        380        390        400        410        420 
NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST AKSQFLKHVD EFSPRAVYTS 

       430        440        450        460        470        480 
GKASSAAGLT AAVVRDEESH EFVIEAGALM LADNGVCCID EFDKMDMRDQ VAIHEAMEQQ 

       490        500        510        520        530        540 
TISITKAGVK ATLNARTSIL AAANPVSGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC 

       550        560        570        580        590        600 
NEVTDYAIAR RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKR 

       610        620        630        640        650        660 
LRQRDGSGVT KSSWRITVRQ LESMIRLSES MARMHCCDEV QPKHVKEAFR LLNKSIIRVE 

       670        680        690        700        710        720 
TPDVNLDQEE EIQMETDEGQ GGVNGHADSP APVNRFNGSS EDASQETVSK PSLRLGFAEY 

       730        740        750        760        770        780 
CRISNLIVLH LRKMEEEEDE SALKRSELVN WYLKEIESEI DSEEELINKK TIIEKVVHRL 

       790        800        810        820 
THYDHVLIEL TQAGLKGSSE GSESYEEDPY LVVNPNYLLE D 

« Hide

References

« Hide 'large scale' references
[1]"Mouse MCM proteins: complex formation and transportation to the nucleus."
Kimura H., Ohtomo T., Yamaguchi M., Ishii A., Sugimoto K.
Genes Cells 1:977-993(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage."
Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III, Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N., Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.
Science 316:1160-1166(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
[4]"SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-643, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Embryonic fibroblast.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D86726 mRNA. Translation: BAA13159.1.
BC050886 mRNA. Translation: AAH50886.2.
BC057584 mRNA. Translation: AAH57584.1.
CCDSCCDS15252.1.
RefSeqNP_032593.1. NM_008567.1.
UniGeneMm.4933.

3D structure databases

ProteinModelPortalP97311.
SMRP97311. Positions 27-652, 710-821.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid201349. 6 interactions.
IntActP97311. 7 interactions.
MINTMINT-4084449.

PTM databases

PhosphoSiteP97311.

Proteomic databases

MaxQBP97311.
PaxDbP97311.
PRIDEP97311.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027601; ENSMUSP00000027601; ENSMUSG00000026355.
GeneID17219.
KEGGmmu:17219.
UCSCuc007cln.1. mouse.

Organism-specific databases

CTD4175.
MGIMGI:1298227. Mcm6.

Phylogenomic databases

eggNOGCOG1241.
HOGENOMHOG000224130.
HOVERGENHBG006334.
InParanoidP97311.
KOK02542.
OMAGTNIRGE.
OrthoDBEOG7CCBQC.
PhylomeDBP97311.
TreeFamTF105646.

Gene expression databases

ArrayExpressP97311.
BgeeP97311.
GenevestigatorP97311.

Family and domain databases

Gene3D2.20.28.10. 1 hit.
2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProIPR008049. MCM6.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004039. Rubredoxin-type_fold.
[Graphical view]
PfamPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSPR01657. MCMFAMILY.
PR01662. MCMPROTEIN6.
SMARTSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSMCM6. mouse.
NextBio291618.
PROP97311.
SOURCESearch...

Entry information

Entry nameMCM6_MOUSE
AccessionPrimary (citable) accession number: P97311
Secondary accession number(s): Q80YQ7
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: July 9, 2014
This is version 122 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot