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Protein

DNA replication licensing factor MCM6

Gene

Mcm6

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi396 – 403ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • DNA replication initiation Source: InterPro
  • DNA unwinding involved in DNA replication Source: MGI

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processCell cycle, DNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-176187 Activation of ATR in response to replication stress
R-MMU-68867 Assembly of the pre-replicative complex
R-MMU-68949 Orc1 removal from chromatin
R-MMU-68962 Activation of the pre-replicative complex
R-MMU-69052 Switching of origins to a post-replicative state

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM6 (EC:3.6.4.12)
Alternative name(s):
Mis5 homolog
Gene namesi
Name:Mcm6
Synonyms:Mcmd6, Mis5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1298227 Mcm6

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001941141 – 821DNA replication licensing factor MCM6Add BLAST821

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei13PhosphoserineBy similarity1
Modified residuei219PhosphoserineBy similarity1
Modified residuei271PhosphoserineCombined sources1
Modified residuei278PhosphothreonineBy similarity1
Modified residuei643N6-acetyllysineCombined sources1
Modified residuei689PhosphoserineCombined sources1
Modified residuei704PhosphoserineCombined sources1
Modified residuei762PhosphoserineCombined sources1
Modified residuei791PhosphothreonineCombined sources1

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

EPDiP97311
PaxDbiP97311
PeptideAtlasiP97311
PRIDEiP97311

PTM databases

iPTMnetiP97311
PhosphoSitePlusiP97311
SwissPalmiP97311

Expressioni

Gene expression databases

BgeeiENSMUSG00000026355
ExpressionAtlasiP97311 baseline and differential
GenevisibleiP97311 MM

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (Probable). May interact with MCM10. Interacts with TIPIN. Interacts with MCMBP (By similarity).By similarityCurated

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdt1Q8R4E92EBI-457132,EBI-457043

GO - Molecular functioni

Protein-protein interaction databases

BioGridi201349, 9 interactors
ComplexPortaliCPX-2941 MCM complex
CORUMiP97311
IntActiP97311, 7 interactors
MINTiP97311
STRINGi10090.ENSMUSP00000027601

Structurei

3D structure databases

ProteinModelPortaliP97311
SMRiP97311
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini346 – 553MCMAdd BLAST208

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi528 – 531Arginine finger4

Sequence similaritiesi

Belongs to the MCM family.Curated

Phylogenomic databases

eggNOGiKOG0480 Eukaryota
COG1241 LUCA
GeneTreeiENSGT00550000074860
HOGENOMiHOG000224130
HOVERGENiHBG006334
InParanoidiP97311
KOiK02542
OMAiFLKYICS
OrthoDBiEOG091G020Q
PhylomeDBiP97311
TreeFamiTF105646

Family and domain databases

InterProiView protein in InterPro
IPR031327 MCM
IPR008049 MCM6
IPR018525 MCM_CS
IPR001208 MCM_dom
IPR027925 MCM_N
IPR033762 MCM_OB
IPR012340 NA-bd_OB-fold
IPR027417 P-loop_NTPase
PANTHERiPTHR11630 PTHR11630, 1 hit
PfamiView protein in Pfam
PF00493 MCM, 1 hit
PF14551 MCM_N, 1 hit
PF17207 MCM_OB, 1 hit
PRINTSiPR01657 MCMFAMILY
PR01662 MCMPROTEIN6
SMARTiView protein in SMART
SM00350 MCM, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
SSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS00847 MCM_1, 1 hit
PS50051 MCM_2, 1 hit

Sequencei

Sequence statusi: Complete.

P97311-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLAAAAEPG AGSQHPEVRD EVAEKCQKLF LDFLEEFQGS DGEIKYLQFA
60 70 80 90 100
EELIRPERNT LVVSFADLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD
110 120 130 140 150
RKEIPFAKDF YVAFQDLPTR HKIRELTSSR IGLLTRISGQ VVRTHPVHPE
160 170 180 190 200
LVSGTFLCLD CQTVIKDVEQ QFKYTQPNIC RNPVCANRKR FLLDTNKSRF
210 220 230 240 250
VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD RCDFTGALIV
260 270 280 290 300
VPDVSKLSTP GARAETNSRV SGADGYETEG IRGLRALGVR DLSYRLVFLA
310 320 330 340 350
CHVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL
360 370 380 390 400
CTSLFPTIHG NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST
410 420 430 440 450
AKSQFLKHVD EFSPRAVYTS GKASSAAGLT AAVVRDEESH EFVIEAGALM
460 470 480 490 500
LADNGVCCID EFDKMDMRDQ VAIHEAMEQQ TISITKAGVK ATLNARTSIL
510 520 530 540 550
AAANPVSGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC NEVTDYAIAR
560 570 580 590 600
RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKR
610 620 630 640 650
LRQRDGSGVT KSSWRITVRQ LESMIRLSES MARMHCCDEV QPKHVKEAFR
660 670 680 690 700
LLNKSIIRVE TPDVNLDQEE EIQMETDEGQ GGVNGHADSP APVNRFNGSS
710 720 730 740 750
EDASQETVSK PSLRLGFAEY CRISNLIVLH LRKMEEEEDE SALKRSELVN
760 770 780 790 800
WYLKEIESEI DSEEELINKK TIIEKVVHRL THYDHVLIEL TQAGLKGSSE
810 820
GSESYEEDPY LVVNPNYLLE D
Length:821
Mass (Da):92,867
Last modified:May 1, 1997 - v1
Checksum:i79E753D2DA218CBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86726 mRNA Translation: BAA13159.1
BC050886 mRNA Translation: AAH50886.2
BC057584 mRNA Translation: AAH57584.1
CCDSiCCDS15252.1
RefSeqiNP_032593.1, NM_008567.2
UniGeneiMm.4933

Genome annotation databases

EnsembliENSMUST00000027601; ENSMUSP00000027601; ENSMUSG00000026355
GeneIDi17219
KEGGimmu:17219
UCSCiuc007cln.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMCM6_MOUSE
AccessioniPrimary (citable) accession number: P97311
Secondary accession number(s): Q80YQ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: June 20, 2018
This is version 158 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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