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P97311

- MCM6_MOUSE

UniProt

P97311 - MCM6_MOUSE

Protein

DNA replication licensing factor MCM6

Gene

Mcm6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi396 – 4038ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ATP-dependent DNA helicase activity Source: Ensembl
    3. protein binding Source: IntAct
    4. single-stranded DNA binding Source: MGI

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. DNA replication initiation Source: InterPro
    3. DNA unwinding involved in DNA replication Source: MGI

    Keywords - Molecular functioni

    Helicase, Hydrolase

    Keywords - Biological processi

    Cell cycle, DNA replication

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_217374. Unwinding of DNA.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA replication licensing factor MCM6 (EC:3.6.4.12)
    Alternative name(s):
    Mis5 homolog
    Gene namesi
    Name:Mcm6
    Synonyms:Mcmd6, Mis5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1298227. Mcm6.

    Subcellular locationi

    Nucleus By similarity
    Note: Binds to chromatin during G1 and detach from it during S phase.By similarity

    GO - Cellular componenti

    1. MCM complex Source: UniProtKB
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 821821DNA replication licensing factor MCM6PRO_0000194114Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei13 – 131PhosphoserineBy similarity
    Modified residuei271 – 2711PhosphoserineBy similarity
    Modified residuei643 – 6431N6-acetyllysine1 Publication
    Modified residuei762 – 7621PhosphoserineBy similarity
    Modified residuei791 – 7911Phosphothreonine1 Publication

    Post-translational modificationi

    O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

    Keywords - PTMi

    Acetylation, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP97311.
    PaxDbiP97311.
    PRIDEiP97311.

    PTM databases

    PhosphoSiteiP97311.

    Expressioni

    Gene expression databases

    ArrayExpressiP97311.
    BgeeiP97311.
    GenevestigatoriP97311.

    Interactioni

    Subunit structurei

    Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). May interact with MCM10. Interacts with TIPIN. Interacts with MCMBP By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Cdt1Q8R4E92EBI-457132,EBI-457043

    Protein-protein interaction databases

    BioGridi201349. 6 interactions.
    IntActiP97311. 7 interactions.
    MINTiMINT-4084449.

    Structurei

    3D structure databases

    ProteinModelPortaliP97311.
    SMRiP97311. Positions 27-652, 710-821.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini346 – 553208MCMAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi528 – 5314Arginine finger

    Sequence similaritiesi

    Belongs to the MCM family.Curated
    Contains 1 MCM domain.Curated

    Phylogenomic databases

    eggNOGiCOG1241.
    HOGENOMiHOG000224130.
    HOVERGENiHBG006334.
    InParanoidiP97311.
    KOiK02542.
    OMAiGTNIRGE.
    OrthoDBiEOG7CCBQC.
    PhylomeDBiP97311.
    TreeFamiTF105646.

    Family and domain databases

    Gene3Di2.20.28.10. 1 hit.
    2.40.50.140. 2 hits.
    3.40.50.300. 1 hit.
    InterProiIPR008049. MCM6.
    IPR018525. MCM_CS.
    IPR001208. MCM_DNA-dep_ATPase.
    IPR027925. MCM_N.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR004039. Rubredoxin-type_fold.
    [Graphical view]
    PfamiPF00493. MCM. 1 hit.
    PF14551. MCM_N. 1 hit.
    [Graphical view]
    PRINTSiPR01657. MCMFAMILY.
    PR01662. MCMPROTEIN6.
    SMARTiSM00350. MCM. 1 hit.
    [Graphical view]
    SUPFAMiSSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEiPS00847. MCM_1. 1 hit.
    PS50051. MCM_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P97311-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDLAAAAEPG AGSQHPEVRD EVAEKCQKLF LDFLEEFQGS DGEIKYLQFA    50
    EELIRPERNT LVVSFADLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD 100
    RKEIPFAKDF YVAFQDLPTR HKIRELTSSR IGLLTRISGQ VVRTHPVHPE 150
    LVSGTFLCLD CQTVIKDVEQ QFKYTQPNIC RNPVCANRKR FLLDTNKSRF 200
    VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD RCDFTGALIV 250
    VPDVSKLSTP GARAETNSRV SGADGYETEG IRGLRALGVR DLSYRLVFLA 300
    CHVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL 350
    CTSLFPTIHG NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST 400
    AKSQFLKHVD EFSPRAVYTS GKASSAAGLT AAVVRDEESH EFVIEAGALM 450
    LADNGVCCID EFDKMDMRDQ VAIHEAMEQQ TISITKAGVK ATLNARTSIL 500
    AAANPVSGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC NEVTDYAIAR 550
    RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKR 600
    LRQRDGSGVT KSSWRITVRQ LESMIRLSES MARMHCCDEV QPKHVKEAFR 650
    LLNKSIIRVE TPDVNLDQEE EIQMETDEGQ GGVNGHADSP APVNRFNGSS 700
    EDASQETVSK PSLRLGFAEY CRISNLIVLH LRKMEEEEDE SALKRSELVN 750
    WYLKEIESEI DSEEELINKK TIIEKVVHRL THYDHVLIEL TQAGLKGSSE 800
    GSESYEEDPY LVVNPNYLLE D 821
    Length:821
    Mass (Da):92,867
    Last modified:May 1, 1997 - v1
    Checksum:i79E753D2DA218CBF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86726 mRNA. Translation: BAA13159.1.
    BC050886 mRNA. Translation: AAH50886.2.
    BC057584 mRNA. Translation: AAH57584.1.
    CCDSiCCDS15252.1.
    RefSeqiNP_032593.1. NM_008567.1.
    UniGeneiMm.4933.

    Genome annotation databases

    EnsembliENSMUST00000027601; ENSMUSP00000027601; ENSMUSG00000026355.
    GeneIDi17219.
    KEGGimmu:17219.
    UCSCiuc007cln.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86726 mRNA. Translation: BAA13159.1 .
    BC050886 mRNA. Translation: AAH50886.2 .
    BC057584 mRNA. Translation: AAH57584.1 .
    CCDSi CCDS15252.1.
    RefSeqi NP_032593.1. NM_008567.1.
    UniGenei Mm.4933.

    3D structure databases

    ProteinModelPortali P97311.
    SMRi P97311. Positions 27-652, 710-821.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 201349. 6 interactions.
    IntActi P97311. 7 interactions.
    MINTi MINT-4084449.

    PTM databases

    PhosphoSitei P97311.

    Proteomic databases

    MaxQBi P97311.
    PaxDbi P97311.
    PRIDEi P97311.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027601 ; ENSMUSP00000027601 ; ENSMUSG00000026355 .
    GeneIDi 17219.
    KEGGi mmu:17219.
    UCSCi uc007cln.1. mouse.

    Organism-specific databases

    CTDi 4175.
    MGIi MGI:1298227. Mcm6.

    Phylogenomic databases

    eggNOGi COG1241.
    HOGENOMi HOG000224130.
    HOVERGENi HBG006334.
    InParanoidi P97311.
    KOi K02542.
    OMAi GTNIRGE.
    OrthoDBi EOG7CCBQC.
    PhylomeDBi P97311.
    TreeFami TF105646.

    Enzyme and pathway databases

    Reactomei REACT_217374. Unwinding of DNA.

    Miscellaneous databases

    ChiTaRSi MCM6. mouse.
    NextBioi 291618.
    PROi P97311.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97311.
    Bgeei P97311.
    Genevestigatori P97311.

    Family and domain databases

    Gene3Di 2.20.28.10. 1 hit.
    2.40.50.140. 2 hits.
    3.40.50.300. 1 hit.
    InterProi IPR008049. MCM6.
    IPR018525. MCM_CS.
    IPR001208. MCM_DNA-dep_ATPase.
    IPR027925. MCM_N.
    IPR012340. NA-bd_OB-fold.
    IPR027417. P-loop_NTPase.
    IPR004039. Rubredoxin-type_fold.
    [Graphical view ]
    Pfami PF00493. MCM. 1 hit.
    PF14551. MCM_N. 1 hit.
    [Graphical view ]
    PRINTSi PR01657. MCMFAMILY.
    PR01662. MCMPROTEIN6.
    SMARTi SM00350. MCM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50249. SSF50249. 1 hit.
    SSF52540. SSF52540. 1 hit.
    PROSITEi PS00847. MCM_1. 1 hit.
    PS50051. MCM_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mouse MCM proteins: complex formation and transportation to the nucleus."
      Kimura H., Ohtomo T., Yamaguchi M., Ishii A., Sugimoto K.
      Genes Cells 1:977-993(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-643, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiMCM6_MOUSE
    AccessioniPrimary (citable) accession number: P97311
    Secondary accession number(s): Q80YQ7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 124 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3