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P97311

- MCM6_MOUSE

UniProt

P97311 - MCM6_MOUSE

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Protein

DNA replication licensing factor MCM6

Gene

Mcm6

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi396 – 4038ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ATP-dependent DNA helicase activity Source: Ensembl
  3. single-stranded DNA binding Source: MGI

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. DNA replication initiation Source: InterPro
  3. DNA unwinding involved in DNA replication Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_217374. Unwinding of DNA.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_245230. Orc1 removal from chromatin.
REACT_254983. Activation of the pre-replicative complex.
REACT_262530. Switching of origins to a post-replicative state.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM6 (EC:3.6.4.12)
Alternative name(s):
Mis5 homolog
Gene namesi
Name:Mcm6
Synonyms:Mcmd6, Mis5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:1298227. Mcm6.

Subcellular locationi

Nucleus By similarity
Note: Binds to chromatin during G1 and detach from it during S phase.By similarity

GO - Cellular componenti

  1. MCM complex Source: UniProtKB
  2. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 821821DNA replication licensing factor MCM6PRO_0000194114Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Modified residuei271 – 2711PhosphoserineBy similarity
Modified residuei643 – 6431N6-acetyllysine1 Publication
Modified residuei762 – 7621PhosphoserineBy similarity
Modified residuei791 – 7911Phosphothreonine1 Publication

Post-translational modificationi

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.By similarity

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP97311.
PaxDbiP97311.
PRIDEiP97311.

PTM databases

PhosphoSiteiP97311.

Expressioni

Gene expression databases

BgeeiP97311.
ExpressionAtlasiP97311. baseline and differential.
GenevestigatoriP97311.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5 (By simililarity). May interact with MCM10. Interacts with TIPIN. Interacts with MCMBP (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cdt1Q8R4E92EBI-457132,EBI-457043

Protein-protein interaction databases

BioGridi201349. 6 interactions.
IntActiP97311. 7 interactions.
MINTiMINT-4084449.

Structurei

3D structure databases

ProteinModelPortaliP97311.
SMRiP97311. Positions 27-652, 710-821.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini346 – 553208MCMAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi528 – 5314Arginine finger

Sequence similaritiesi

Belongs to the MCM family.Curated
Contains 1 MCM domain.Curated

Phylogenomic databases

eggNOGiCOG1241.
HOGENOMiHOG000224130.
HOVERGENiHBG006334.
InParanoidiP97311.
KOiK02542.
OMAiGTNIRGE.
OrthoDBiEOG7CCBQC.
PhylomeDBiP97311.
TreeFamiTF105646.

Family and domain databases

Gene3Di2.20.28.10. 1 hit.
2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR008049. MCM6.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004039. Rubredoxin-type_fold.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01662. MCMPROTEIN6.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97311-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDLAAAAEPG AGSQHPEVRD EVAEKCQKLF LDFLEEFQGS DGEIKYLQFA
60 70 80 90 100
EELIRPERNT LVVSFADLEQ FNQQLSTTIQ EEFYRVYPYL CRALKTFVKD
110 120 130 140 150
RKEIPFAKDF YVAFQDLPTR HKIRELTSSR IGLLTRISGQ VVRTHPVHPE
160 170 180 190 200
LVSGTFLCLD CQTVIKDVEQ QFKYTQPNIC RNPVCANRKR FLLDTNKSRF
210 220 230 240 250
VDFQKVRIQE TQAELPRGSI PRSLEVILRA EAVESAQAGD RCDFTGALIV
260 270 280 290 300
VPDVSKLSTP GARAETNSRV SGADGYETEG IRGLRALGVR DLSYRLVFLA
310 320 330 340 350
CHVAPTNPRF GGKELRDEEQ TAESIKNQMT VKEWEKVFEM SQDKNLYHNL
360 370 380 390 400
CTSLFPTIHG NDEVKRGVLL MLFGGVPKTT GEGTSLRGDI NVCIVGDPST
410 420 430 440 450
AKSQFLKHVD EFSPRAVYTS GKASSAAGLT AAVVRDEESH EFVIEAGALM
460 470 480 490 500
LADNGVCCID EFDKMDMRDQ VAIHEAMEQQ TISITKAGVK ATLNARTSIL
510 520 530 540 550
AAANPVSGHY DRSKSLKQNI NLSAPIMSRF DLFFILVDEC NEVTDYAIAR
560 570 580 590 600
RIVDLHSRIE ESIDRVYSLD DIRRYLLFAR QFKPKISKES EDFIVEQYKR
610 620 630 640 650
LRQRDGSGVT KSSWRITVRQ LESMIRLSES MARMHCCDEV QPKHVKEAFR
660 670 680 690 700
LLNKSIIRVE TPDVNLDQEE EIQMETDEGQ GGVNGHADSP APVNRFNGSS
710 720 730 740 750
EDASQETVSK PSLRLGFAEY CRISNLIVLH LRKMEEEEDE SALKRSELVN
760 770 780 790 800
WYLKEIESEI DSEEELINKK TIIEKVVHRL THYDHVLIEL TQAGLKGSSE
810 820
GSESYEEDPY LVVNPNYLLE D
Length:821
Mass (Da):92,867
Last modified:May 1, 1997 - v1
Checksum:i79E753D2DA218CBF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86726 mRNA. Translation: BAA13159.1.
BC050886 mRNA. Translation: AAH50886.2.
BC057584 mRNA. Translation: AAH57584.1.
CCDSiCCDS15252.1.
RefSeqiNP_032593.1. NM_008567.1.
UniGeneiMm.4933.

Genome annotation databases

EnsembliENSMUST00000027601; ENSMUSP00000027601; ENSMUSG00000026355.
GeneIDi17219.
KEGGimmu:17219.
UCSCiuc007cln.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86726 mRNA. Translation: BAA13159.1 .
BC050886 mRNA. Translation: AAH50886.2 .
BC057584 mRNA. Translation: AAH57584.1 .
CCDSi CCDS15252.1.
RefSeqi NP_032593.1. NM_008567.1.
UniGenei Mm.4933.

3D structure databases

ProteinModelPortali P97311.
SMRi P97311. Positions 27-652, 710-821.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201349. 6 interactions.
IntActi P97311. 7 interactions.
MINTi MINT-4084449.

PTM databases

PhosphoSitei P97311.

Proteomic databases

MaxQBi P97311.
PaxDbi P97311.
PRIDEi P97311.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027601 ; ENSMUSP00000027601 ; ENSMUSG00000026355 .
GeneIDi 17219.
KEGGi mmu:17219.
UCSCi uc007cln.1. mouse.

Organism-specific databases

CTDi 4175.
MGIi MGI:1298227. Mcm6.

Phylogenomic databases

eggNOGi COG1241.
HOGENOMi HOG000224130.
HOVERGENi HBG006334.
InParanoidi P97311.
KOi K02542.
OMAi GTNIRGE.
OrthoDBi EOG7CCBQC.
PhylomeDBi P97311.
TreeFami TF105646.

Enzyme and pathway databases

Reactomei REACT_217374. Unwinding of DNA.
REACT_230841. Assembly of the pre-replicative complex.
REACT_232236. Activation of ATR in response to replication stress.
REACT_236939. Removal of licensing factors from origins.
REACT_245230. Orc1 removal from chromatin.
REACT_254983. Activation of the pre-replicative complex.
REACT_262530. Switching of origins to a post-replicative state.

Miscellaneous databases

ChiTaRSi Mcm6. mouse.
NextBioi 291618.
PROi P97311.
SOURCEi Search...

Gene expression databases

Bgeei P97311.
ExpressionAtlasi P97311. baseline and differential.
Genevestigatori P97311.

Family and domain databases

Gene3Di 2.20.28.10. 1 hit.
2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR008049. MCM6.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
IPR004039. Rubredoxin-type_fold.
[Graphical view ]
Pfami PF00493. MCM. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view ]
PRINTSi PR01657. MCMFAMILY.
PR01662. MCMPROTEIN6.
SMARTi SM00350. MCM. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse MCM proteins: complex formation and transportation to the nucleus."
    Kimura H., Ohtomo T., Yamaguchi M., Ishii A., Sugimoto K.
    Genes Cells 1:977-993(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-791, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  4. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-643, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiMCM6_MOUSE
AccessioniPrimary (citable) accession number: P97311
Secondary accession number(s): Q80YQ7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: November 26, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3