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P97310

- MCM2_MOUSE

UniProt

P97310 - MCM2_MOUSE

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Protein

DNA replication licensing factor MCM2

Gene
Mcm2, Bm28, Cdcl1, Kiaa0030, Mcmd2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri329 – 35527C4-type Reviewed predictionAdd
BLAST
Nucleotide bindingi523 – 5308ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA helicase activity Source: InterPro
  3. DNA replication origin binding Source: MGI
  4. histone binding Source: MGI
  5. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. cellular response to interleukin-4 Source: MGI
  3. DNA replication initiation Source: MGI
  4. DNA unwinding involved in DNA replication Source: MGI
  5. nucleosome assembly Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase

Keywords - Biological processi

Cell cycle, DNA replication

Keywords - Ligandi

ATP-binding, DNA-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiREACT_217374. Unwinding of DNA.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA replication licensing factor MCM2 (EC:3.6.4.12)
Alternative name(s):
Minichromosome maintenance protein 2 homolog
Nuclear protein BM28
Gene namesi
Name:Mcm2
Synonyms:Bm28, Cdcl1, Kiaa0030, Mcmd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:105380. Mcm2.

Subcellular locationi

Nucleus Inferred

GO - Cellular componenti

  1. chromatin Source: Ensembl
  2. MCM complex Source: UniProtKB
  3. nuclear origin of replication recognition complex Source: MGI
  4. nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi237 – 2371L → P: Impairs interaction with KAT7. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 904903DNA replication licensing factor MCM2PRO_0000194088Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei12 – 121Phosphoserine1 Publication
Modified residuei21 – 211Phosphoserine3 Publications
Modified residuei26 – 261Phosphoserine By similarity
Modified residuei27 – 271Phosphoserine2 Publications
Modified residuei39 – 391Phosphothreonine By similarity
Modified residuei40 – 401Phosphoserine; by CDC7 By similarity
Modified residuei41 – 411Phosphoserine By similarity
Modified residuei53 – 531Phosphoserine; by CDC7 By similarity
Modified residuei59 – 591Phosphothreonine By similarity
Modified residuei108 – 1081Phosphoserine By similarity
Modified residuei139 – 1391Phosphoserine2 Publications
Modified residuei140 – 1401Phosphoserine2 Publications
Modified residuei216 – 2161N6-acetyllysine By similarity
Modified residuei381 – 3811Phosphoserine By similarity

Post-translational modificationi

Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication By similarity.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP97310.
PaxDbiP97310.
PRIDEiP97310.

PTM databases

PhosphoSiteiP97310.

Miscellaneous databases

PMAP-CutDBP97310.

Expressioni

Gene expression databases

ArrayExpressiP97310.
BgeeiP97310.
GenevestigatoriP97310.

Interactioni

Subunit structurei

Component of the MCM2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order MCM2-MCM6-MCM4-MCM7-MCM3-MCM5. Interacts with KAT7 and DBF4. May interact with MCM10 By similarity.2 Publications

Protein-protein interaction databases

BioGridi201346. 4 interactions.
DIPiDIP-33057N.
IntActiP97310. 7 interactions.
MINTiMINT-4101561.

Structurei

3D structure databases

ProteinModelPortaliP97310.
SMRiP97310. Positions 199-798.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini473 – 680208MCMAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 257256Interaction with KAT7Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi655 – 6584Arginine finger

Sequence similaritiesi

Belongs to the MCM family.
Contains 1 MCM domain.

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiCOG1241.
GeneTreeiENSGT00740000115337.
HOVERGENiHBG106398.
InParanoidiP97310.
KOiK02540.
OMAiNMEETVY.
OrthoDBiEOG71VSRZ.
PhylomeDBiP97310.
TreeFamiTF300772.

Family and domain databases

Gene3Di2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR008045. MCM2.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00493. MCM. 1 hit.
PF12619. MCM2_N. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view]
PRINTSiPR01657. MCMFAMILY.
PR01658. MCMPROTEIN2.
SMARTiSM00350. MCM. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97310-1 [UniParc]FASTAAdd to Basket

« Hide

MAESSESLSA SSPARQRRRI SDPLTSSPGR SSRRADALTS SPGRDLPPFE    50
DESEGLLGTE GPMEEEEDGE ELIGDGMERD YRPIPELDVY EAEGLALDDE 100
DVEELTASQR EAAERTMRQR DREAGRGLGR MRRGLLYDSS EEDEERPARK 150
RRHVERATED GEEDEEMIES IENLEDLKGH SVREWVSMAG PRLEIHHRFK 200
NFLRTHVDSH GHNVFKERIS DMCKENRESL VVNYEDLAAR EHVLAYFLPE 250
APAELLQIFD EAALEVVLAM YPKYDRITNH IHVRISHLPL VEELRSLRQL 300
HLNQLIRTSG VVTSCTGVLP QLSMVKYNCS KCNFVLGPFC QSQNQEVKPG 350
SCPECQSAGP FEINMEETIY QNYQRIRIQE SPGKVAAGRL PRSKDAILLA 400
DLVDSCKPGD EIELTGIYHN NYDGSLNTAN GFPVFATIIL ANHVAKKDNK 450
VAVGELTDED VKMITGLSKD QQIGEKIFAS IAPSIYGHED IKRGLALALF 500
GGEPKNPGGK HKVRGDINVL LCGDPGTAKS QFLKYIEKVS SRAIFTTGQG 550
ASAVGLTAYV QRHPVSREWT LEAGALVLAD RGVCLIDEFD KMNDQDRTSI 600
HEAMEQQSIS ISKAGIVTSL QARCTVIAAA NPIGGRYDPS LTFSENVDLT 650
EPIISRFDVL CVVRDTVDPV QDEMLARFVV GSHVRHHPSN KKDEGLTNGG 700
TLEPAMPNTY GVEPLPQEVL KKYIIYAKER VRPKLNQMDQ DKVARMYSDL 750
RKESMATGSI PITVRHIESM IRMAEAHARM HLRDYVMEDD VNMAIRVMME 800
SFIDTQKFSV MRSMRKTFAR YLSFRRDNND LLLFILKQLV AEQVTYQRNR 850
FGAQQDTIEI PEKDLMDKAR QINIHNLSAF YDSDLFKFNK FSRDLKRKLI 900
LQQF 904
Length:904
Mass (Da):102,078
Last modified:April 13, 2004 - v3
Checksum:i7B61C13DAD1CAC58
GO

Sequence cautioni

The sequence BAC97849.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701E → G in AAC16250. 1 Publication
Sequence conflicti135 – 1351L → P in AAC16250. 1 Publication
Sequence conflicti185 – 1851W → R in BAA22148. 1 Publication
Sequence conflicti271 – 2711Y → H in AAC16250. 1 Publication
Sequence conflicti506 – 5061N → D in AAC36510. 1 Publication
Sequence conflicti521 – 5211L → S in AAC36510. 1 Publication
Sequence conflicti648 – 6481D → N in AAC36510. 1 Publication
Sequence conflicti822 – 8221L → I in AAC16250. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86725 mRNA. Translation: BAA22148.1.
AF004105 mRNA. Translation: AAC16250.1.
AK129039 mRNA. Translation: BAC97849.1. Different initiation.
AK088156 mRNA. Translation: BAC40178.1.
BC055318 mRNA. Translation: AAH55318.1.
U89403 mRNA. Translation: AAC36510.1.
CCDSiCCDS39554.1.
PIRiT10067.
RefSeqiNP_032590.2. NM_008564.2.
UniGeneiMm.16711.

Genome annotation databases

EnsembliENSMUST00000058011; ENSMUSP00000061923; ENSMUSG00000002870.
GeneIDi17216.
KEGGimmu:17216.
UCSCiuc009cvx.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D86725 mRNA. Translation: BAA22148.1 .
AF004105 mRNA. Translation: AAC16250.1 .
AK129039 mRNA. Translation: BAC97849.1 . Different initiation.
AK088156 mRNA. Translation: BAC40178.1 .
BC055318 mRNA. Translation: AAH55318.1 .
U89403 mRNA. Translation: AAC36510.1 .
CCDSi CCDS39554.1.
PIRi T10067.
RefSeqi NP_032590.2. NM_008564.2.
UniGenei Mm.16711.

3D structure databases

ProteinModelPortali P97310.
SMRi P97310. Positions 199-798.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 201346. 4 interactions.
DIPi DIP-33057N.
IntActi P97310. 7 interactions.
MINTi MINT-4101561.

PTM databases

PhosphoSitei P97310.

Proteomic databases

MaxQBi P97310.
PaxDbi P97310.
PRIDEi P97310.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000058011 ; ENSMUSP00000061923 ; ENSMUSG00000002870 .
GeneIDi 17216.
KEGGi mmu:17216.
UCSCi uc009cvx.2. mouse.

Organism-specific databases

CTDi 4171.
MGIi MGI:105380. Mcm2.
Rougei Search...

Phylogenomic databases

eggNOGi COG1241.
GeneTreei ENSGT00740000115337.
HOVERGENi HBG106398.
InParanoidi P97310.
KOi K02540.
OMAi NMEETVY.
OrthoDBi EOG71VSRZ.
PhylomeDBi P97310.
TreeFami TF300772.

Enzyme and pathway databases

Reactomei REACT_217374. Unwinding of DNA.

Miscellaneous databases

ChiTaRSi MCM2. mouse.
NextBioi 291606.
PMAP-CutDB P97310.
PROi P97310.
SOURCEi Search...

Gene expression databases

ArrayExpressi P97310.
Bgeei P97310.
Genevestigatori P97310.

Family and domain databases

Gene3Di 2.40.50.140. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR008045. MCM2.
IPR018525. MCM_CS.
IPR001208. MCM_DNA-dep_ATPase.
IPR027925. MCM_N.
IPR012340. NA-bd_OB-fold.
IPR027417. P-loop_NTPase.
[Graphical view ]
Pfami PF00493. MCM. 1 hit.
PF12619. MCM2_N. 1 hit.
PF14551. MCM_N. 1 hit.
[Graphical view ]
PRINTSi PR01657. MCMFAMILY.
PR01658. MCMPROTEIN2.
SMARTi SM00350. MCM. 1 hit.
[Graphical view ]
SUPFAMi SSF50249. SSF50249. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS00847. MCM_1. 1 hit.
PS50051. MCM_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse MCM proteins: complex formation and transportation to the nucleus."
    Kimura H., Ohtomo T., Yamaguchi M., Ishii A., Sugimoto K.
    Genes Cells 1:977-993(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 217-224; 376-384 AND 477-487.
  2. Kimura H.
    Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Clone 10d/BM28 (CDCL1), an early S-phase protein, is an important growth regulator of melanoma."
    Spanjaard R.A., Lee P.J., Sarkar S., Goedegebuure P.S., Eberlein T.J.
    Cancer Res. 57:5122-5128(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Melanoma.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene: III. The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S., Saga Y., Nagase T., Ohara O., Koga H.
    DNA Res. 10:167-180(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Embryonic tail.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Thymus.
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  7. "Expressed genes in interleukin-4 treated B cells identified by cDNA representational difference analysis."
    Chu C.C., Paul W.E.
    Mol. Immunol. 35:487-502(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 477-668.
    Strain: BALB/c.
    Tissue: Spleen.
  8. "Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes."
    Lepke M., Puetter V., Staib C., Kneissl M., Berger C., Hoehn K., Nanda I., Schmid M., Grummt F.
    Mol. Gen. Genet. 262:220-229(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH DBF4.
    Strain: C57BL/6J.
    Tissue: Egg and Embryo.
  9. "Replication factors MCM2 and ORC1 interact with the histone acetyltransferase HBO1."
    Burke T.W., Cook J.G., Asano M., Nevins J.R.
    J. Biol. Chem. 276:15397-15408(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH KAT7, MUTAGENESIS OF LEU-237.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  11. "A differential phosphoproteomic analysis of retinoic acid-treated P19 cells."
    Smith J.C., Duchesne M.A., Tozzi P., Ethier M., Figeys D.
    J. Proteome Res. 6:3174-3186(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Teratocarcinoma.
  12. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-21; SER-27; SER-139 AND SER-140, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  13. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-12; SER-21; SER-27; SER-139 AND SER-140, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiMCM2_MOUSE
AccessioniPrimary (citable) accession number: P97310
Secondary accession number(s): O08971, O89057, Q8C2R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: April 13, 2004
Last modified: September 3, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi