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Protein

Mesoderm posterior protein 1

Gene

Mesp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.2 Publications

GO - Molecular functioni

  • enhancer binding Source: BHF-UCL
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: BHF-UCL
  • transcription regulatory region DNA binding Source: MGI

GO - Biological processi

  • cardiac atrium formation Source: MGI
  • cardiac cell fate determination Source: BHF-UCL
  • cardiac muscle cell differentiation Source: MGI
  • cardiac vascular smooth muscle cell differentiation Source: MGI
  • cardiac ventricle formation Source: MGI
  • cardioblast anterior-lateral migration Source: BHF-UCL
  • cardioblast migration Source: MGI
  • cardioblast migration to the midline involved in heart field formation Source: BHF-UCL
  • embryonic heart tube morphogenesis Source: BHF-UCL
  • endothelial cell differentiation Source: MGI
  • gastrulation Source: BHF-UCL
  • growth involved in heart morphogenesis Source: BHF-UCL
  • heart looping Source: BHF-UCL
  • heart morphogenesis Source: MGI
  • lateral mesoderm development Source: BHF-UCL
  • mesodermal cell migration Source: MGI
  • negative regulation of endodermal cell fate specification Source: BHF-UCL
  • negative regulation of mesodermal cell fate specification Source: BHF-UCL
  • negative regulation of transcription, DNA-templated Source: BHF-UCL
  • neurogenesis Source: MGI
  • Notch signaling pathway Source: UniProtKB-KW
  • positive regulation of heart induction by negative regulation of canonical Wnt signaling pathway Source: BHF-UCL
  • positive regulation of hepatocyte differentiation Source: BHF-UCL
  • positive regulation of Notch signaling pathway Source: BHF-UCL
  • positive regulation of Notch signaling pathway involved in heart induction Source: BHF-UCL
  • positive regulation of striated muscle cell differentiation Source: BHF-UCL
  • positive regulation of transcription, DNA-templated Source: BHF-UCL
  • positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • secondary heart field specification Source: BHF-UCL
  • signal transduction involved in regulation of gene expression Source: MGI
  • sinoatrial node cell differentiation Source: MGI
  • sinus venosus morphogenesis Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Notch signaling pathway, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mesoderm posterior protein 1
Gene namesi
Name:Mesp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:107785. Mesp1.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: BHF-UCL
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice embryos exhibit defects in single heart tube formation, due to a delay in mesodermal migration. However, the cells eventually acquired migratory activity and gave rise to an abnormal heart tube. Mice lacking Mesp1 and Mesp2 die around 9.5 dpc. The major defect is the apparent lack of any mesodermal layer between endoderm and ectoderm and a defect in the migratory activity of mesodermal cells. It seems that a compensatory mechanism exists in which Mesp1 expression is up-regulated in the absence of Mesp2. Mesp1 may be involved in the rescue of somitogenesis in the null Mesp2 embryos.3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 243243Mesoderm posterior protein 1PRO_0000304405Add
BLAST

Proteomic databases

PaxDbiP97309.
PRIDEiP97309.

Expressioni

Tissue specificityi

No expression was detected in adult tissues except the testis. Expression in the testis was regulated developmentally; expressed 2 weeks after birth, and increases, reaching the full expression level in mature testes.1 Publication

Developmental stagei

The earliest expression is detected at the junction of the epiblast and extraembryonic ectoderm, which is the initiation site for gastrulation. Around 6.5-6.75 dpc, expression becomes evident as gastrulation progresses. Expression does not spread over all mesodermal cells; it is seen only in a fraction of cells, particularly those in the early emergence group destined to become the extraembryonic mesoderm and the most posterior part of the tailbud. Expression remains for a while at the base of the allantois, and then spreads out to the lateral margins of the tail bud mesoderm. Expressed in the anterior presomitic mesoderm in a broad or thin stripe pattern. Expressed in the paraxial mesoderm during somitogenesis. Expression disappears before 8.5 dpc.4 Publications

Inductioni

Expression is induced by Notch signaling.

Gene expression databases

BgeeiP97309.
CleanExiMM_MESP1.
GenevisibleiP97309. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032760.

Structurei

3D structure databases

ProteinModelPortaliP97309.
SMRiP97309. Positions 88-131.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini76 – 13055bHLHPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi153 – 1575CPLCP

Sequence similaritiesi

Contains 1 bHLH (basic helix-loop-helix) domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00530000063712.
HOGENOMiHOG000113566.
HOVERGENiHBG094949.
InParanoidiP97309.
KOiK09076.
OMAiPLSESWM.
OrthoDBiEOG79PJQZ.
PhylomeDBiP97309.
TreeFamiTF325707.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97309-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQPLCEPRS ESWILSPAGR QPPMPSDGNS VCSPAWSSDP WDGAQASSPA
60 70 80 90 100
PPCARPARRA GTPGRRGTHG SRLGSGQRQS ASEREKLRMR TLARALHELR
110 120 130 140 150
RFLPPSVAPT GQNLTKIETL RLAIRYIGHL SAVLGLSEDN LRRQRHAVSP
160 170 180 190 200
RGCPLCPDSD LAQSQSLGPR LSPAVCSGVS WGSPPAYPRP RVAAESWDPS
210 220 230 240
FLYAETASQE RQEMEPSPSS PLFSSDMLAL LETWTPPQEW PPA
Length:243
Mass (Da):26,386
Last modified:September 11, 2007 - v2
Checksum:iD82F72C0B0FB05EE
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti170 – 1701R → G in BAA12041 (PubMed:8787751).Curated
Sequence conflicti202 – 2021L → Q in BAA12041 (PubMed:8787751).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83674 mRNA. Translation: BAA12041.1.
BC125505 mRNA. Translation: AAI25506.1.
BC125507 mRNA. Translation: AAI25508.1.
CCDSiCCDS21387.1.
RefSeqiNP_032614.2. NM_008588.2.
UniGeneiMm.1404.

Genome annotation databases

EnsembliENSMUST00000032760; ENSMUSP00000032760; ENSMUSG00000030544.
GeneIDi17292.
KEGGimmu:17292.
UCSCiuc009hzb.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D83674 mRNA. Translation: BAA12041.1.
BC125505 mRNA. Translation: AAI25506.1.
BC125507 mRNA. Translation: AAI25508.1.
CCDSiCCDS21387.1.
RefSeqiNP_032614.2. NM_008588.2.
UniGeneiMm.1404.

3D structure databases

ProteinModelPortaliP97309.
SMRiP97309. Positions 88-131.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032760.

Proteomic databases

PaxDbiP97309.
PRIDEiP97309.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032760; ENSMUSP00000032760; ENSMUSG00000030544.
GeneIDi17292.
KEGGimmu:17292.
UCSCiuc009hzb.2. mouse.

Organism-specific databases

CTDi55897.
MGIiMGI:107785. Mesp1.

Phylogenomic databases

eggNOGiKOG4029. Eukaryota.
ENOG411227D. LUCA.
GeneTreeiENSGT00530000063712.
HOGENOMiHOG000113566.
HOVERGENiHBG094949.
InParanoidiP97309.
KOiK09076.
OMAiPLSESWM.
OrthoDBiEOG79PJQZ.
PhylomeDBiP97309.
TreeFamiTF325707.

Miscellaneous databases

PROiP97309.
SOURCEiSearch...

Gene expression databases

BgeeiP97309.
CleanExiMM_MESP1.
GenevisibleiP97309. MM.

Family and domain databases

Gene3Di4.10.280.10. 1 hit.
InterProiIPR011598. bHLH_dom.
[Graphical view]
PfamiPF00010. HLH. 1 hit.
[Graphical view]
SMARTiSM00353. HLH. 1 hit.
[Graphical view]
SUPFAMiSSF47459. SSF47459. 1 hit.
PROSITEiPS50888. BHLH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "MesP1: a novel basic helix-loop-helix protein expressed in the nascent mesodermal cells during mouse gastrulation."
    Saga Y., Hata N., Kobayashi S., Magnuson T., Seldin M.F., Taketo M.M.
    Development 122:2769-2778(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: ICR.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "MesP1 and MesP2 are essential for the development of cardiac mesoderm."
    Kitajima S., Takagi A., Inoue T., Saga Y.
    Development 127:3215-3226(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  4. "Differential contributions of Mesp1 and Mesp2 to the epithelialization and rostro-caudal patterning of somites."
    Takahashi Y., Kitajima S., Inoue T., Kanno J., Saga Y.
    Development 132:787-796(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  5. "Appropriate suppression of Notch signaling by Mesp factors is essential for stripe pattern formation leading to segment boundary formation."
    Takahashi Y., Yasuhiko Y., Kitajima S., Kanno J., Saga Y.
    Dev. Biol. 304:593-603(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiMESP1_MOUSE
AccessioniPrimary (citable) accession number: P97309
Secondary accession number(s): Q059W2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: September 11, 2007
Last modified: July 6, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.