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P97305 (NFAC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nuclear factor of activated T-cells, cytoplasmic 3
Short name=NF-ATc3
Short name=NFATc3
Alternative name(s):
NFATx
T-cell transcription factor NFAT4
Short name=NF-AT4
Gene names
Name:Nfatc3
Synonyms:Nfat4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length1075 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Acts as a regulator of transcriptional activation. Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2 By similarity.

Subunit structure

Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. NFATC proteins bind to DNA as monomers.

Subcellular location

Cytoplasm. Nucleus. Note: Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription.

Tissue specificity

Expressed in thymus. Weakly expressed in muscle, spleen and kidney. Also expressed in lymph node.

Domain

Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors By similarity.

Post-translational modification

Phosphorylated by NFATC-kinase; dephosphorylated by calcineurin By similarity.

Sequence similarities

Contains 1 RHD (Rel-like) domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   Coding sequence diversityAlternative splicing
   DomainRepeat
   LigandDNA-binding
   Molecular functionActivator
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular respiration

Inferred from mutant phenotype PubMed 12750314. Source: MGI

cellular response to lithium ion

Inferred from direct assay PubMed 20530871. Source: MGI

heart development

Inferred from genetic interaction PubMed 12750314PubMed 17229811. Source: MGI

muscle cell development

Inferred from genetic interaction PubMed 11439183. Source: MGI

patterning of blood vessels

Inferred from genetic interaction PubMed 11439183. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

smooth muscle cell differentiation

Inferred from genetic interaction PubMed 11439183. Source: MGI

transcription, DNA-dependent

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytosol

Inferred from direct assay PubMed 20530871. Source: MGI

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from direct assay PubMed 16260608. Source: MGI

chromatin binding

Inferred from direct assay PubMed 16998587. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from direct assay PubMed 16260608PubMed 16998587. Source: MGI

Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform X1 (identifier: P97305-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform X2 (identifier: P97305-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1035-1075: VNEIIGRDMSQISVSQATEVMRDTPLPGPASPDLMTSHSAH → DQLISDLEHQPSGSTEKWSNHSEFSCPVPFWRI
Isoform Delta-X (identifier: P97305-3)

The sequence of this isoform differs from the canonical sequence as follows:
     468-497: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 10751074Nuclear factor of activated T-cells, cytoplasmic 3
PRO_0000205181

Regions

Repeat207 – 223171
Repeat236 – 252172
Repeat292 – 308173
Domain415 – 596182RHD
DNA binding444 – 4518 By similarity
Region109 – 1146Calcineurin-binding
Region207 – 3081023 X SP repeats
Motif273 – 2753Nuclear localization signal
Motif686 – 6883Nuclear localization signal
Motif1031 – 104010Nuclear export signal
Compositional bias24 – 296Poly-Pro

Amino acid modifications

Modified residue21N-acetylthreonine By similarity

Natural variations

Alternative sequence468 – 49730Missing in isoform Delta-X.
VSP_005603
Alternative sequence1035 – 107541VNEII…SHSAH → DQLISDLEHQPSGSTEKWSN HSEFSCPVPFWRI in isoform X2.
VSP_005604

Experimental info

Sequence conflict12 – 3423LDFKL…GSRPA → PRRVLFSVSAQLPSRTRPGP SDL Ref.2
Sequence conflict57 – 615TTPLC → NSSLG in AAA93249. Ref.2
Sequence conflict681P → LQ Ref.2
Sequence conflict82 – 832SH → GY in AAA93249. Ref.2
Sequence conflict90 – 934CEIP → GDIS in AAA93249. Ref.2
Sequence conflict1131F → I in AAA93249. Ref.2
Sequence conflict121 – 1244QQEL → HQGT in AAA93249. Ref.2
Sequence conflict1321Q → H in AAA93249. Ref.2
Sequence conflict1401F → Y in AAA93249. Ref.2
Sequence conflict6411G → R in AAA93249. Ref.2
Sequence conflict6461V → A in AAA93249. Ref.2
Sequence conflict707 – 73428REDTD…SSETG → KEKTQICLQFHLCLCLILPS PEALLRDR in AAA93249. Ref.2
Sequence conflict7461G → S in AAA93249. Ref.2
Sequence conflict10751H → Q in AAA93249. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform X1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 4ED38C9AA6F452BB

FASTA1,075115,451
        10         20         30         40         50         60 
MTTANCGAHD ELDFKLVFGE DGAPAPPPPG SRPADLEPDD CASIYIFNVD PPPSTLTTPL 

        70         80         90        100        110        120 
CLPHHGLPSH SSVLSPSFQL QSHKNYEGTC EIPESKYSPL GGPKPFECPS IQFTSISPNC 

       130        140        150        160        170        180 
QQELDAHEDD LQINDPEREF LERPSRDHLY LPLEPSYRES SLSPSPASSI SSRSWFSDAS 

       190        200        210        220        230        240 
SCESLSHIYD DVDSELNEAA ARFTLGSPLT SPGGSPGGCP GEESWHQQYG SGHSLSPRQS 

       250        260        270        280        290        300 
PCHSPRSSIT DENWLSPRPA SGPSSRPTSP CGKRRHSSAE VCYAGSLSPH HSPVPSPGHS 

       310        320        330        340        350        360 
PRGSVTEDTW LTAPVHTGSG LSPAPFPFQY CVETDIPLKT RKTSEDQAAI LPGKLEICSD 

       370        380        390        400        410        420 
DQGNLSPSRE TSVDDGLGSQ YPLKKDSSGD QFLSVPSPFT WSKPKPGHTP IFRTSSLPPL 

       430        440        450        460        470        480 
DWPLPTHFGQ CELKIEVQPK THHRAHYETE GSRGAVKAST GGHPVVKLLG YSEKPINLQM 

       490        500        510        520        530        540 
FIGTADDRYL RPHAFYQVHR ITGKTVATAS QEIIIASTKV LEIPLLPENN MSASIDCAGI 

       550        560        570        580        590        600 
LKLRNSDIEL RKGETDIGRK NTRVRLVFRV HIPQPSGKVL SLQIASIPVE CSQRSAQELP 

       610        620        630        640        650        660 
HIEKYSINSC SVNGGHEMIV TGSNFLPESK IIFLEKGQDG GPHWEVEGKI IREKCQGAHI 

       670        680        690        700        710        720 
VLEVPPYHNP AVTSAVQVHF YLCNGKRKKS QSQRFTYTPV LMKQEQREDT DLPSVPSLPV 

       730        740        750        760        770        780 
PHSAQAQRPS SETGHPHDRA MSAPGGLLCQ VQPAYTSMVA STHLPQLQCR DEGAGKEQHI 

       790        800        810        820        830        840 
ATSSVMHQPF QVTPTSPIGS SYQSIQTSMY NGPTCLPVNP ASSQEFDPVL FQQDAALSSL 

       850        860        870        880        890        900 
VNLGCQPLSP IPFHSSNSDA TGHLLAHSPH SVQTPPHLQS MGYHCSNAGQ TALSSPVADQ 

       910        920        930        940        950        960 
ITGQPSSHLQ PITYCPSHPG SATAASPAAS HPLASSPISG PSSPQLQPMP YQSPSSGTAS 

       970        980        990       1000       1010       1020 
SPSPTTRMHS GQHSTQAQST GQGGLSVPSS LVCHSLCDPA SFPPGGATVS IKPEPEDQEP 

      1030       1040       1050       1060       1070 
NFATIGLQDI TLDDVNEIIG RDMSQISVSQ ATEVMRDTPL PGPASPDLMT SHSAH

« Hide

Isoform X2 [UniParc].

Checksum: D3F228D966EE4C3C
Show »

FASTA1,067114,932
Isoform Delta-X [UniParc].

Checksum: 8C7DB1A257C2BE6E
Show »

FASTA1,045111,911

References

[1]"NFATc3, a lymphoid-specific NFATc family member that is calcium-regulated and exhibits distinct DNA binding specificity."
Ho S.N., Thomas D.J., Timmerman L.A., Li X., Francke U., Crabtree G.R.
J. Biol. Chem. 270:19898-19907(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM X1).
Tissue: Thymus.
[2]"Calcineurin-dependent nuclear translocation of a murine transcription factor NFATx: molecular cloning and functional characterization."
Liu J., Koyano-Nakagawa N., Amasaki Y., Saito-Ohara F., Ikeuchi T., Imai S., Takano T., Arai N., Yokota T., Arai K.
Mol. Biol. Cell 8:157-170(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 12-1075 (ISOFORMS X1; X2 AND DELTA-X).
Tissue: Thymic lymphoma.
[3]"Generic signals and specific outcomes: signaling through Ca2+, calcineurin, and NF-AT."
Crabtree G.R.
Cell 96:611-614(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		D85612 mRNA. Translation: BAA12833.1.
U28807 mRNA. Translation: AAA93249.1.
IPIIPI00123845.
IPI00225469.
IPI00225470.
UniGeneMm.383185.

3D structure databases

ProteinModelPortalP97305.
SMRP97305. Positions 419-700.
ModBaseSearch...

PTM databases

PhosphoSiteP97305.

Proteomic databases

PRIDEP97305.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:103296. Nfatc3.

Phylogenomic databases

eggNOGNOG70055.
HOGENOMHOG000231780.
HOVERGENHBG069754.
InParanoidP97305.

Gene expression databases

GenevestigatorP97305.
GermOnlineENSMUSG00000031902. Mus musculus.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
2.60.40.340. 1 hit.
InterProIPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
IPR002909. IPT_TIG_rcpt.
IPR008366. NFAT.
IPR008967. p53-like_TF_DNA-bd.
IPR011539. RHD.
[Graphical view]
PANTHERPTHR12533. PTHR12533. 1 hit.
PfamPF00554. RHD. 1 hit.
[Graphical view]
PRINTSPR01789. NUCFACTORATC.
SMARTSM00429. IPT. 1 hit.
[Graphical view]
SUPFAMSSF81296. Ig_E-set. 1 hit.
SSF49417. P53_like_DNA_bnd. 1 hit.
PROSITEPS01204. REL_1. False negative.
PS50254. REL_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

SOURCESearch...

Entry information

Entry nameNFAC3_MOUSE
AccessionPrimary (citable) accession number: P97305
Secondary accession number(s): Q60896
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1997
Last modified: April 3, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents