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P97303

- BACH2_MOUSE

UniProt

P97303 - BACH2_MOUSE

Protein

Transcription regulator protein BACH2

Gene

Bach2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 3 (18 Sep 2013)
      Previous versions | rss
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    Functioni

    Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK.

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: MGI
    3. RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
    4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: NTNU_SB
    5. sequence-specific DNA binding transcription factor activity Source: MGI

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    2. regulation of transcription, DNA-templated Source: MGI

    Keywords - Molecular functioni

    Activator, Repressor

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Transcription regulator protein BACH2
    Alternative name(s):
    BTB and CNC homolog 2
    Gene namesi
    Name:Bach2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:894679. Bach2.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 PublicationPROSITE-ProRule annotation
    Note: Nucleocytoplasmic shuttling is controlled by phosphorylation.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: MGI

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 839839Transcription regulator protein BACH2PRO_0000076457Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi20 – 20Interchain; redox-activeBy similarity
    Modified residuei520 – 5201Phosphoserine; by RPS6KB1By similarity

    Post-translational modificationi

    The reversible disulfide bond may provide mechanism to regulate the activity in oxidative stress responses.By similarity
    Phosphorylation at Ser-520 downstream of the PI-3K pathway promotes nuclear export.By similarity

    Keywords - PTMi

    Disulfide bond, Phosphoprotein

    Proteomic databases

    PRIDEiP97303.

    PTM databases

    PhosphoSiteiP97303.

    Expressioni

    Tissue specificityi

    Expression restricted to monocytes and neuronal cells.

    Gene expression databases

    ArrayExpressiP97303.
    BgeeiP97303.
    CleanExiMM_BACH2.
    GenevestigatoriP97303.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked By similarity. Heterodimer of BACH2 and Maf-related transcription factors.By similarity

    Protein-protein interaction databases

    IntActiP97303. 1 interaction.
    MINTiMINT-1728942.
    STRINGi10090.ENSMUSP00000103815.

    Structurei

    3D structure databases

    ProteinModelPortaliP97303.
    SMRiP97303. Positions 8-129, 608-705.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 10367BTBPROSITE-ProRule annotationAdd
    BLAST
    Domaini645 – 70864bZIPPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni650 – 66617Basic motifPROSITE-ProRule annotationAdd
    BLAST
    Regioni670 – 6778Leucine-zipperPROSITE-ProRule annotation

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi819 – 83921Nuclear export signalAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi162 – 1687Poly-Glu

    Sequence similaritiesi

    Belongs to the bZIP family. CNC subfamily.Curated
    Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
    Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG276808.
    GeneTreeiENSGT00550000074399.
    HOGENOMiHOG000231712.
    HOVERGENiHBG050655.
    KOiK09042.
    OMAiLHRYCPV.
    OrthoDBiEOG7WMCHX.
    PhylomeDBiP97303.
    TreeFamiTF326681.

    Family and domain databases

    Gene3Di1.10.880.10. 1 hit.
    3.30.710.10. 1 hit.
    InterProiIPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR004827. bZIP.
    IPR004826. bZIP_Maf.
    IPR008917. TF_DNA-bd.
    [Graphical view]
    PfamiPF00651. BTB. 1 hit.
    PF03131. bZIP_Maf. 1 hit.
    [Graphical view]
    SMARTiSM00338. BRLZ. 1 hit.
    SM00225. BTB. 1 hit.
    [Graphical view]
    SUPFAMiSSF47454. SSF47454. 1 hit.
    SSF54695. SSF54695. 1 hit.
    PROSITEiPS50097. BTB. 1 hit.
    PS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P97303-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MSVDEKPGSP MYVYESTVHC ANILLGLNDQ RKKDILCDVT LIVERKEFRA    50
    HRAVLAACSE YFWQALVGQT KDDLVVSLPE EVTARGFGPL LQFAYTAKLL 100
    LSRENIREVI RCAEFLRMHN LEDSCFSFLQ TQLLNREDGL FVCRKDSACQ 150
    RPQEDHGNSA GEEEEEEETM DSETARMACA TDQMLPDPIS FEATAIPVAE 200
    KEEALLPESE VPTDTKENSE KGALTQYPRY KKYQLACTKN VYSAPSHGTS 250
    GFASTFSEDS PGNSLKPGLP MGQIKSEPPS EETEEESITL CLSGDETDIK 300
    DRPGDVEMDR KQPSPAPTPS TPTGAACLDR SRSVSSPSCL RSLFGITKGV 350
    ESTGLPSTSQ QPLVRSSACP FNKGISQGDL KTDYTPLAGN YGQPHVGQKD 400
    VSNFAMGSPL RGPGPETLCK QEGELDRRSV IFSASACDQP NTPVHSYSAV 450
    SNLDKDLSEP VPKSLWVGAG QSLPSSQAYS HSGLMADHLP GRIRPNTSCP 500
    VPIKVCPRSP PLETRTRTSS SCSSYSYAED GSGGSPCSLP LCEFSSSPCS 550
    QGARFLATEH QEPGLMGDGM YNQVRPQIKC EQSYGTNSSD ESGSFSEADS 600
    ESCPVQDRGQ EVKLPFPVDQ ITDLPRNDFQ MMIKMHKLTS EQLEFIHDIR 650
    RRSKNRIAAQ RCRKRKLDCI QNLECEIRKL VCEKEKLLSE RNHLKACMGE 700
    LLDNFSCLSQ EVCRDIQSPE QIQALHRYCP VLIPMDLPGA SVNPPPVGVE 750
    QSLAPSPCAV GGSVPCCLEP GAAPPGLPWV PSNTSENCTS GRRLEGSDPG 800
    TFSERGPPLE ARSQSVTVDF CQEMTEKCTT DEQPRKDYA 839
    Length:839
    Mass (Da):91,836
    Last modified:September 18, 2013 - v3
    Checksum:i8A5321A68A07653D
    GO
    Isoform 2 (identifier: P97303-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         421-543: Missing.

    Show »
    Length:716
    Mass (Da):78,817
    Checksum:i703BCE4305A70352
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti220 – 2201E → G in AAI31643. (PubMed:15489334)Curated
    Sequence conflicti317 – 3171P → R in BAA13138. (PubMed:8887638)Curated
    Sequence conflicti322 – 3221P → R in BAA13138. (PubMed:8887638)Curated
    Sequence conflicti420 – 4201K → E in BAA13138. (PubMed:8887638)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei421 – 543123Missing in isoform 2. 1 PublicationVSP_047933Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86604 mRNA. Translation: BAA13138.1.
    AL732547 Genomic DNA. No translation available.
    AL831746 Genomic DNA. Translation: CAM26623.1.
    BC131642 mRNA. Translation: AAI31643.1.
    CCDSiCCDS51135.1. [P97303-1]
    RefSeqiNP_001103131.1. NM_001109661.1. [P97303-1]
    XP_006537630.1. XM_006537567.1. [P97303-1]
    UniGeneiMm.491223.

    Genome annotation databases

    EnsembliENSMUST00000037416; ENSMUSP00000043693; ENSMUSG00000040270. [P97303-2]
    ENSMUST00000108180; ENSMUSP00000103815; ENSMUSG00000040270. [P97303-1]
    ENSMUST00000171600; ENSMUSP00000131592; ENSMUSG00000040270. [P97303-1]
    GeneIDi12014.
    KEGGimmu:12014.
    UCSCiuc012dbf.1. mouse.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D86604 mRNA. Translation: BAA13138.1 .
    AL732547 Genomic DNA. No translation available.
    AL831746 Genomic DNA. Translation: CAM26623.1 .
    BC131642 mRNA. Translation: AAI31643.1 .
    CCDSi CCDS51135.1. [P97303-1 ]
    RefSeqi NP_001103131.1. NM_001109661.1. [P97303-1 ]
    XP_006537630.1. XM_006537567.1. [P97303-1 ]
    UniGenei Mm.491223.

    3D structure databases

    ProteinModelPortali P97303.
    SMRi P97303. Positions 8-129, 608-705.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P97303. 1 interaction.
    MINTi MINT-1728942.
    STRINGi 10090.ENSMUSP00000103815.

    PTM databases

    PhosphoSitei P97303.

    Proteomic databases

    PRIDEi P97303.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000037416 ; ENSMUSP00000043693 ; ENSMUSG00000040270 . [P97303-2 ]
    ENSMUST00000108180 ; ENSMUSP00000103815 ; ENSMUSG00000040270 . [P97303-1 ]
    ENSMUST00000171600 ; ENSMUSP00000131592 ; ENSMUSG00000040270 . [P97303-1 ]
    GeneIDi 12014.
    KEGGi mmu:12014.
    UCSCi uc012dbf.1. mouse.

    Organism-specific databases

    CTDi 60468.
    MGIi MGI:894679. Bach2.

    Phylogenomic databases

    eggNOGi NOG276808.
    GeneTreei ENSGT00550000074399.
    HOGENOMi HOG000231712.
    HOVERGENi HBG050655.
    KOi K09042.
    OMAi LHRYCPV.
    OrthoDBi EOG7WMCHX.
    PhylomeDBi P97303.
    TreeFami TF326681.

    Miscellaneous databases

    NextBioi 280225.
    PROi P97303.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97303.
    Bgeei P97303.
    CleanExi MM_BACH2.
    Genevestigatori P97303.

    Family and domain databases

    Gene3Di 1.10.880.10. 1 hit.
    3.30.710.10. 1 hit.
    InterProi IPR000210. BTB/POZ-like.
    IPR011333. BTB/POZ_fold.
    IPR013069. BTB_POZ.
    IPR004827. bZIP.
    IPR004826. bZIP_Maf.
    IPR008917. TF_DNA-bd.
    [Graphical view ]
    Pfami PF00651. BTB. 1 hit.
    PF03131. bZIP_Maf. 1 hit.
    [Graphical view ]
    SMARTi SM00338. BRLZ. 1 hit.
    SM00225. BTB. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47454. SSF47454. 1 hit.
    SSF54695. SSF54695. 1 hit.
    PROSITEi PS50097. BTB. 1 hit.
    PS50217. BZIP. 1 hit.
    PS00036. BZIP_BASIC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Bach proteins belong to a novel family of BTB-basic leucine zipper transcription factors that interact with MafK and regulate transcription through the NF-E2 site."
      Oyake T., Itoh K., Motohashi H., Hayashi N., Hoshino H., Nishizawa M., Yamamoto M., Igarashi K.
      Mol. Cell. Biol. 16:6083-6095(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
      Strain: BALB/c.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    4. "Oxidative stress abolishes leptomycin B-sensitive nuclear export of transcription repressor Bach2 that counteracts activation of Maf recognition element."
      Hoshino H., Kobayashi A., Yoshida M., Kudo N., Oyake T., Motohashi H., Hayashi N., Yamamoto M., Igarashi K.
      J. Biol. Chem. 275:15370-15376(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, NUCLEAR EXPORT SIGNAL.
    5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiBACH2_MOUSE
    AccessioniPrimary (citable) accession number: P97303
    Secondary accession number(s): A2ANU8, A2ANU9, A2RRI0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: September 18, 2013
    Last modified: October 1, 2014
    This is version 119 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3