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Protein

Transcription regulator protein BACH2

Gene

Bach2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional regulator that acts as repressor or activator (PubMed:8887638). Binds to Maf recognition elements (MARE) (PubMed:8887638). Plays an important role in coordinating transcription activation and repression by MAFK (PubMed:8887638). Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1 (By similarity). Positively regulates the nuclear import of actin (PubMed:26021350).By similarity1 Publication

GO - Molecular functioni

  • DNA binding Source: MGI
  • RNA polymerase II distal enhancer sequence-specific DNA binding Source: NTNU_SB
  • transcriptional repressor activity, RNA polymerase II distal enhancer sequence-specific binding Source: NTNU_SB
  • transcription factor activity, sequence-specific DNA binding Source: MGI

GO - Biological processi

  • negative regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • protein ubiquitination Source: GOC
  • regulation of transcription, DNA-templated Source: MGI
  • single-organism nuclear import Source: UniProtKB
  • transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Transcription regulator protein BACH2
Alternative name(s):
BTB and CNC homolog 2
Gene namesi
Name:Bach2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:894679. Bach2.

Subcellular locationi

GO - Cellular componenti

  • Cul3-RING ubiquitin ligase complex Source: GO_Central
  • cytoplasm Source: UniProtKB-SubCell
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000764571 – 839Transcription regulator protein BACH2Add BLAST839

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi20Interchain; redox-activeBy similarity
Modified residuei314PhosphoserineCombined sources1
Modified residuei520PhosphoserineBy similarity1

Post-translational modificationi

The reversible disulfide bond may provide a mechanism to regulate the activity in oxidative stress responses.By similarity
Phosphorylation at Ser-520 downstream of the PI-3K pathway promotes nuclear export.By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP97303.
PaxDbiP97303.
PeptideAtlasiP97303.
PRIDEiP97303.

PTM databases

iPTMnetiP97303.
PhosphoSitePlusiP97303.

Expressioni

Tissue specificityi

Detected in brain and spleen.1 Publication

Developmental stagei

In brain, expression is lower in the adult than in the neonate.1 Publication

Gene expression databases

BgeeiENSMUSG00000040270.
CleanExiMM_BACH2.
GenevisibleiP97303. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Heterodimer of BACH2 and Maf-related transcription factors (PubMed:8887638).By similarity1 Publication

Protein-protein interaction databases

BioGridi198295. 6 interactors.
IntActiP97303. 1 interactor.
MINTiMINT-1728942.
STRINGi10090.ENSMUSP00000103815.

Structurei

3D structure databases

ProteinModelPortaliP97303.
SMRiP97303.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 103BTBPROSITE-ProRule annotationAdd BLAST67
Domaini645 – 708bZIPPROSITE-ProRule annotationAdd BLAST64

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni650 – 666Basic motifPROSITE-ProRule annotationAdd BLAST17
Regioni670 – 677Leucine-zipperPROSITE-ProRule annotation8

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi819 – 839Nuclear export signalAdd BLAST21

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi162 – 168Poly-Glu7

Sequence similaritiesi

Belongs to the bZIP family. CNC subfamily.Curated
Contains 1 BTB (POZ) domain.PROSITE-ProRule annotation
Contains 1 bZIP (basic-leucine zipper) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG410ISV4. Eukaryota.
ENOG410ZDVB. LUCA.
GeneTreeiENSGT00830000128250.
HOGENOMiHOG000231712.
HOVERGENiHBG050655.
InParanoidiP97303.
KOiK09042.
OMAiSESCPVQ.
OrthoDBiEOG091G02EB.
PhylomeDBiP97303.
TreeFamiTF326681.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR011333. SKP1/BTB/POZ.
IPR008917. TF_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P97303-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSVDEKPGSP MYVYESTVHC ANILLGLNDQ RKKDILCDVT LIVERKEFRA
60 70 80 90 100
HRAVLAACSE YFWQALVGQT KDDLVVSLPE EVTARGFGPL LQFAYTAKLL
110 120 130 140 150
LSRENIREVI RCAEFLRMHN LEDSCFSFLQ TQLLNREDGL FVCRKDSACQ
160 170 180 190 200
RPQEDHGNSA GEEEEEEETM DSETARMACA TDQMLPDPIS FEATAIPVAE
210 220 230 240 250
KEEALLPESE VPTDTKENSE KGALTQYPRY KKYQLACTKN VYSAPSHGTS
260 270 280 290 300
GFASTFSEDS PGNSLKPGLP MGQIKSEPPS EETEEESITL CLSGDETDIK
310 320 330 340 350
DRPGDVEMDR KQPSPAPTPS TPTGAACLDR SRSVSSPSCL RSLFGITKGV
360 370 380 390 400
ESTGLPSTSQ QPLVRSSACP FNKGISQGDL KTDYTPLAGN YGQPHVGQKD
410 420 430 440 450
VSNFAMGSPL RGPGPETLCK QEGELDRRSV IFSASACDQP NTPVHSYSAV
460 470 480 490 500
SNLDKDLSEP VPKSLWVGAG QSLPSSQAYS HSGLMADHLP GRIRPNTSCP
510 520 530 540 550
VPIKVCPRSP PLETRTRTSS SCSSYSYAED GSGGSPCSLP LCEFSSSPCS
560 570 580 590 600
QGARFLATEH QEPGLMGDGM YNQVRPQIKC EQSYGTNSSD ESGSFSEADS
610 620 630 640 650
ESCPVQDRGQ EVKLPFPVDQ ITDLPRNDFQ MMIKMHKLTS EQLEFIHDIR
660 670 680 690 700
RRSKNRIAAQ RCRKRKLDCI QNLECEIRKL VCEKEKLLSE RNHLKACMGE
710 720 730 740 750
LLDNFSCLSQ EVCRDIQSPE QIQALHRYCP VLIPMDLPGA SVNPPPVGVE
760 770 780 790 800
QSLAPSPCAV GGSVPCCLEP GAAPPGLPWV PSNTSENCTS GRRLEGSDPG
810 820 830
TFSERGPPLE ARSQSVTVDF CQEMTEKCTT DEQPRKDYA
Length:839
Mass (Da):91,836
Last modified:September 18, 2013 - v3
Checksum:i8A5321A68A07653D
GO
Isoform 2 (identifier: P97303-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     421-543: Missing.

Show »
Length:716
Mass (Da):78,817
Checksum:i703BCE4305A70352
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti220E → G in AAI31643 (PubMed:15489334).Curated1
Sequence conflicti317P → R in BAA13138 (PubMed:8887638).Curated1
Sequence conflicti322P → R in BAA13138 (PubMed:8887638).Curated1
Sequence conflicti420K → E in BAA13138 (PubMed:8887638).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_047933421 – 543Missing in isoform 2. 1 PublicationAdd BLAST123

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86604 mRNA. Translation: BAA13138.1.
AL732547 Genomic DNA. No translation available.
AL831746 Genomic DNA. Translation: CAM26623.1.
BC131642 mRNA. Translation: AAI31643.1.
CCDSiCCDS51135.1. [P97303-1]
RefSeqiNP_001103131.1. NM_001109661.1. [P97303-1]
XP_006537630.1. XM_006537567.3. [P97303-1]
XP_011248211.1. XM_011249909.2. [P97303-1]
UniGeneiMm.491223.

Genome annotation databases

EnsembliENSMUST00000037416; ENSMUSP00000043693; ENSMUSG00000040270. [P97303-2]
ENSMUST00000108180; ENSMUSP00000103815; ENSMUSG00000040270. [P97303-1]
ENSMUST00000171600; ENSMUSP00000131592; ENSMUSG00000040270. [P97303-1]
GeneIDi12014.
KEGGimmu:12014.
UCSCiuc012dbf.1. mouse. [P97303-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D86604 mRNA. Translation: BAA13138.1.
AL732547 Genomic DNA. No translation available.
AL831746 Genomic DNA. Translation: CAM26623.1.
BC131642 mRNA. Translation: AAI31643.1.
CCDSiCCDS51135.1. [P97303-1]
RefSeqiNP_001103131.1. NM_001109661.1. [P97303-1]
XP_006537630.1. XM_006537567.3. [P97303-1]
XP_011248211.1. XM_011249909.2. [P97303-1]
UniGeneiMm.491223.

3D structure databases

ProteinModelPortaliP97303.
SMRiP97303.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198295. 6 interactors.
IntActiP97303. 1 interactor.
MINTiMINT-1728942.
STRINGi10090.ENSMUSP00000103815.

PTM databases

iPTMnetiP97303.
PhosphoSitePlusiP97303.

Proteomic databases

MaxQBiP97303.
PaxDbiP97303.
PeptideAtlasiP97303.
PRIDEiP97303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037416; ENSMUSP00000043693; ENSMUSG00000040270. [P97303-2]
ENSMUST00000108180; ENSMUSP00000103815; ENSMUSG00000040270. [P97303-1]
ENSMUST00000171600; ENSMUSP00000131592; ENSMUSG00000040270. [P97303-1]
GeneIDi12014.
KEGGimmu:12014.
UCSCiuc012dbf.1. mouse. [P97303-1]

Organism-specific databases

CTDi60468.
MGIiMGI:894679. Bach2.

Phylogenomic databases

eggNOGiENOG410ISV4. Eukaryota.
ENOG410ZDVB. LUCA.
GeneTreeiENSGT00830000128250.
HOGENOMiHOG000231712.
HOVERGENiHBG050655.
InParanoidiP97303.
KOiK09042.
OMAiSESCPVQ.
OrthoDBiEOG091G02EB.
PhylomeDBiP97303.
TreeFamiTF326681.

Miscellaneous databases

ChiTaRSiBach2. mouse.
PROiP97303.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040270.
CleanExiMM_BACH2.
GenevisibleiP97303. MM.

Family and domain databases

Gene3Di1.10.880.10. 1 hit.
InterProiIPR000210. BTB/POZ_dom.
IPR004827. bZIP.
IPR004826. bZIP_Maf.
IPR011333. SKP1/BTB/POZ.
IPR008917. TF_DNA-bd.
[Graphical view]
PfamiPF00651. BTB. 1 hit.
PF03131. bZIP_Maf. 1 hit.
[Graphical view]
SMARTiSM00338. BRLZ. 1 hit.
SM00225. BTB. 1 hit.
[Graphical view]
SUPFAMiSSF47454. SSF47454. 1 hit.
SSF54695. SSF54695. 1 hit.
PROSITEiPS50097. BTB. 1 hit.
PS50217. BZIP. 1 hit.
PS00036. BZIP_BASIC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBACH2_MOUSE
AccessioniPrimary (citable) accession number: P97303
Secondary accession number(s): A2ANU8, A2ANU9, A2RRI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: September 18, 2013
Last modified: November 30, 2016
This is version 139 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.