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Protein

Secreted frizzled-related protein 2

Gene

Sfrp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

GO - Molecular functioni

  1. endopeptidase activator activity Source: MGI
  2. metalloenzyme activator activity Source: MGI
  3. receptor agonist activity Source: BHF-UCL
  4. Wnt-protein binding Source: MGI

GO - Biological processi

  1. anterior/posterior pattern specification Source: MGI
  2. apoptotic process Source: MGI
  3. bone morphogenesis Source: MGI
  4. cardiac left ventricle morphogenesis Source: MGI
  5. cartilage development Source: MGI
  6. cellular response to extracellular stimulus Source: MGI
  7. cellular response to X-ray Source: UniProtKB
  8. chondrocyte development Source: MGI
  9. collagen fibril organization Source: MGI
  10. convergent extension involved in axis elongation Source: MGI
  11. development of primary male sexual characteristics Source: MGI
  12. digestive tract morphogenesis Source: MGI
  13. embryonic digit morphogenesis Source: MGI
  14. hematopoietic stem cell proliferation Source: UniProtKB
  15. male gonad development Source: MGI
  16. negative regulation of BMP signaling pathway Source: MGI
  17. negative regulation of canonical Wnt signaling pathway Source: MGI
  18. negative regulation of cardiac muscle cell apoptotic process Source: MGI
  19. negative regulation of cell growth Source: MGI
  20. negative regulation of cell migration Source: UniProtKB
  21. negative regulation of cell proliferation Source: MGI
  22. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  23. negative regulation of dermatome development Source: BHF-UCL
  24. negative regulation of epithelial cell proliferation Source: MGI
  25. negative regulation of epithelial to mesenchymal transition Source: MGI
  26. negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
  27. negative regulation of gene expression Source: UniProtKB
  28. negative regulation of JUN kinase activity Source: MGI
  29. negative regulation of mesodermal cell fate specification Source: MGI
  30. negative regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  31. negative regulation of planar cell polarity pathway involved in axis elongation Source: MGI
  32. negative regulation of transcription, DNA-templated Source: MGI
  33. negative regulation of Wnt signaling pathway Source: BHF-UCL
  34. neural tube closure Source: MGI
  35. neural tube development Source: MGI
  36. outflow tract morphogenesis Source: MGI
  37. patterning of blood vessels Source: BHF-UCL
  38. planar cell polarity pathway involved in neural tube closure Source: MGI
  39. positive regulation of angiogenesis Source: BHF-UCL
  40. positive regulation of apoptotic process Source: MGI
  41. positive regulation of canonical Wnt signaling pathway Source: BHF-UCL
  42. positive regulation of catenin import into nucleus Source: BHF-UCL
  43. positive regulation of cell growth Source: UniProtKB
  44. positive regulation of cell proliferation Source: UniProtKB
  45. positive regulation of endopeptidase activity Source: MGI
  46. positive regulation of fat cell differentiation Source: MGI
  47. positive regulation of osteoblast differentiation Source: MGI
  48. positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
  49. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  50. post-anal tail morphogenesis Source: MGI
  51. regulation of apoptotic process Source: MGI
  52. regulation of cell proliferation Source: MGI
  53. regulation of establishment of planar polarity Source: MGI
  54. regulation of stem cell division Source: UniProtKB
  55. regulation of Wnt signaling pathway Source: UniProtKB
  56. response to drug Source: UniProtKB
  57. response to nutrient Source: Ensembl
  58. sclerotome development Source: BHF-UCL
  59. somitogenesis Source: MGI
  60. Wnt signaling pathway involved in somitogenesis Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiREACT_210612. negative regulation of TCF-dependent signaling by WNT ligand antagonists.

Protein family/group databases

MEROPSiI93.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Secreted frizzled-related protein 2
Short name:
sFRP-2
Alternative name(s):
Protein SDF5
Secreted apoptosis-related protein 1
Short name:
SARP-1
Gene namesi
Name:Sfrp2
Synonyms:Sarp1, Sdf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 3

Organism-specific databases

MGIiMGI:108078. Sfrp2.

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: Reactome
  2. extracellular space Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 295271Secreted frizzled-related protein 2PRO_0000032543Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi40 ↔ 103By similarity
Disulfide bondi50 ↔ 96By similarity
Disulfide bondi87 ↔ 125By similarity
Disulfide bondi114 ↔ 152By similarity
Disulfide bondi118 ↔ 142By similarity
Disulfide bondi172 ↔ 245By similarity
Disulfide bondi175 ↔ 247By similarity
Disulfide bondi190 ↔ 295By similarity

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP97299.

PTM databases

PhosphoSiteiP97299.

Expressioni

Tissue specificityi

Highly expressed in the eye. Weaker expression in heart and lung.1 Publication

Developmental stagei

During kidney development, expressed at E10.5 in the mesonephric tubules, and at E12.5 strongly expressed in the comma shaped bodies and surrounding ureter stalk. In E14.5 kidney, expressed in the S-shaped bodies. In the developing nervous system, expressed in the presumptive hindbrain and the ventral part of the neural tube from E8.0 onwards. At E9.5, expression is additionally detected in the mesonephros and the optic vesicle. E10 expression is found in the second and third branchial cleft, in the eye, the ventral neural tube and specific rhombomeres and prosomers. E10.5 brain shows specific expression in the basal and alar plate. In developing eye, expressed at E9.0 in the optic placode, and at E9.5 in the optic vesicle. By E10.5, expression found in the lens vesicle and the inner layer of the invaginating optic vesicle. Strong expression at E14.5 in the anterior lens epithelium, decreasing thereafter. Expression also found in the prospective neural retina. In developing limbs, expression found at E11.5 in the shoulder and at the distal end of the cartilaginous condensation. At E12.5, expressed in the foot and hand paddle extending along the digital rays. Expressed, at E13.5 and E14.5, in the forelimb and hindlimb where the interphalangeal joints will develop. Also expressed at E14.5 between the sternal bands and where the ribs contact the sternum. In other developing structures, expression found at E11.5, in the maxillary and mandibular component of the first branchial arch, and later, in the loose mesenchyme surrounding cartilage and epithelia of the skull as well as in the whisker follicles. Also expressed in developing teeth, with the highest levels at E15.5 and E16.5 in the mesenchyme and the dental epithelium of the developing molars. Expressed in development smooth muscle surrounding the esophagus at E11.5, the dorsal aorta and the ductus arteriosus at E14.5, and the ureter stalk at E15.5.2 Publications

Gene expression databases

BgeeiP97299.
CleanExiMM_SFRP2.
ExpressionAtlasiP97299. baseline and differential.
GenevestigatoriP97299.

Interactioni

Protein-protein interaction databases

BioGridi203142. 9 interactions.
DIPiDIP-59486N.

Structurei

3D structure databases

ProteinModelPortaliP97299.
SMRiP97299. Positions 49-150.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 155121FZPROSITE-ProRule annotationAdd
BLAST
Domaini172 – 295124NTRPROSITE-ProRule annotationAdd
BLAST

Domaini

The FZ domain is involved in binding with Wnt ligands.By similarity

Sequence similaritiesi

Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation
Contains 1 NTR domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG240950.
HOGENOMiHOG000231058.
HOVERGENiHBG052928.
InParanoidiP97299.
KOiK02176.
OMAiTSLKRWQ.
OrthoDBiEOG789CBZ.
PhylomeDBiP97299.
TreeFamiTF350133.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026558. SFRP2.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF45. PTHR11309:SF45. 1 hit.
PfamiPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97299-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPRGPASLLL LVLASHCCLG SARGLFLFGQ PDFSYKRSNC KPIPANLQLC
60 70 80 90 100
HGIEYQNMRL PNLLGHETMK EVLEQAGAWI PLVMKQCHPD TKKFLCSLFA
110 120 130 140 150
PVCLDDLDET IQPCHSLCVQ VKDRCAPVMS AFGFPWPDML ECDRFPQDND
160 170 180 190 200
LCIPLASSDH LLPATEEAPK VCEACKTKNE DDNDIMETLC KNDFALKIKV
210 220 230 240 250
KEITYINRDT KIILETKSKT IYKLNGVSER DLKKSVLWLK DSLQCTCEEM
260 270 280 290
NDINAPYLVM GQKQGGELVI TSVKRWQKGQ REFKRISRSI RKLQC
Length:295
Mass (Da):33,469
Last modified:March 15, 2005 - v2
Checksum:i2D287C177C974C62
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti38 – 381S → T in AAB70795 (PubMed:9391078).Curated
Sequence conflicti119 – 1191V → M in BAA09053 (PubMed:8938438).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50462 mRNA. Translation: BAA09053.1.
U88567 mRNA. Translation: AAC53146.1.
AF017989 mRNA. Translation: AAB70795.1.
BC014722 mRNA. Translation: AAH14722.1.
CCDSiCCDS17434.1.
RefSeqiNP_033170.1. NM_009144.2.
UniGeneiMm.19155.

Genome annotation databases

EnsembliENSMUST00000029625; ENSMUSP00000029625; ENSMUSG00000027996.
GeneIDi20319.
KEGGimmu:20319.
UCSCiuc008ppl.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50462 mRNA. Translation: BAA09053.1.
U88567 mRNA. Translation: AAC53146.1.
AF017989 mRNA. Translation: AAB70795.1.
BC014722 mRNA. Translation: AAH14722.1.
CCDSiCCDS17434.1.
RefSeqiNP_033170.1. NM_009144.2.
UniGeneiMm.19155.

3D structure databases

ProteinModelPortaliP97299.
SMRiP97299. Positions 49-150.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203142. 9 interactions.
DIPiDIP-59486N.

Protein family/group databases

MEROPSiI93.001.

PTM databases

PhosphoSiteiP97299.

Proteomic databases

PRIDEiP97299.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000029625; ENSMUSP00000029625; ENSMUSG00000027996.
GeneIDi20319.
KEGGimmu:20319.
UCSCiuc008ppl.2. mouse.

Organism-specific databases

CTDi6423.
MGIiMGI:108078. Sfrp2.

Phylogenomic databases

eggNOGiNOG240950.
HOGENOMiHOG000231058.
HOVERGENiHBG052928.
InParanoidiP97299.
KOiK02176.
OMAiTSLKRWQ.
OrthoDBiEOG789CBZ.
PhylomeDBiP97299.
TreeFamiTF350133.

Enzyme and pathway databases

ReactomeiREACT_210612. negative regulation of TCF-dependent signaling by WNT ligand antagonists.

Miscellaneous databases

ChiTaRSiSfrp2. mouse.
NextBioi298111.
PROiP97299.
SOURCEiSearch...

Gene expression databases

BgeeiP97299.
CleanExiMM_SFRP2.
ExpressionAtlasiP97299. baseline and differential.
GenevestigatoriP97299.

Family and domain databases

Gene3Di1.10.2000.10. 1 hit.
InterProiIPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026558. SFRP2.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERiPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF45. PTHR11309:SF45. 1 hit.
PfamiPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTiSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
SUPFAMiSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEiPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of novel secreted and membrane proteins isolated by the signal sequence trap method."
    Shirozu M., Tada H., Tashiro K., Nakamura T., Lopez N.D., Nazarea M., Hamada T., Sato T., Nakano T., Honjo T.
    Genomics 37:273-280(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Bone marrow stroma.
  2. "A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors."
    Rattner A., Hsieh J.-C., Smallwood P.M., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J.
    Proc. Natl. Acad. Sci. U.S.A. 94:2859-2863(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Strain: C57BL/6J.
    Tissue: Embryonic eye.
  3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary tumor.
  5. "Developmental expression patterns of mouse sFRP genes encoding members of the secreted frizzled related protein family."
    Leimeister C., Bach A., Gessler M.
    Mech. Dev. 75:29-42(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: DEVELOPMENTAL STAGE.
  6. Cited for: DEVELOPMENTAL STAGE.

Entry informationi

Entry nameiSFRP2_MOUSE
AccessioniPrimary (citable) accession number: P97299
Secondary accession number(s): O08862, O35297
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: March 4, 2015
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.