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P97299

- SFRP2_MOUSE

UniProt

P97299 - SFRP2_MOUSE

Protein

Secreted frizzled-related protein 2

Gene

Sfrp2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (15 Mar 2005)
      Previous versions | rss
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    Functioni

    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

    GO - Molecular functioni

    1. endopeptidase activator activity Source: MGI
    2. metalloenzyme activator activity Source: MGI
    3. PDZ domain binding Source: RefGenome
    4. protein binding Source: MGI
    5. receptor agonist activity Source: BHF-UCL
    6. Wnt-activated receptor activity Source: RefGenome
    7. Wnt-protein binding Source: MGI

    GO - Biological processi

    1. anterior/posterior pattern specification Source: MGI
    2. apoptotic process Source: MGI
    3. bone morphogenesis Source: MGI
    4. brain development Source: RefGenome
    5. cardiac left ventricle morphogenesis Source: Ensembl
    6. cartilage development Source: MGI
    7. cellular response to extracellular stimulus Source: MGI
    8. cellular response to X-ray Source: UniProtKB
    9. chondrocyte development Source: MGI
    10. collagen fibril organization Source: MGI
    11. convergent extension involved in axis elongation Source: MGI
    12. development of primary male sexual characteristics Source: MGI
    13. digestive tract morphogenesis Source: MGI
    14. embryonic digit morphogenesis Source: MGI
    15. hematopoietic stem cell proliferation Source: UniProtKB
    16. male gonad development Source: MGI
    17. negative regulation of BMP signaling pathway Source: MGI
    18. negative regulation of canonical Wnt signaling pathway Source: MGI
    19. negative regulation of cardiac muscle cell apoptotic process Source: MGI
    20. negative regulation of cell growth Source: Ensembl
    21. negative regulation of cell migration Source: UniProtKB
    22. negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
    23. negative regulation of dermatome development Source: BHF-UCL
    24. negative regulation of epithelial cell proliferation Source: Ensembl
    25. negative regulation of epithelial to mesenchymal transition Source: Ensembl
    26. negative regulation of extrinsic apoptotic signaling pathway via death domain receptors Source: BHF-UCL
    27. negative regulation of gene expression Source: UniProtKB
    28. negative regulation of JUN kinase activity Source: MGI
    29. negative regulation of mesodermal cell fate specification Source: MGI
    30. negative regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
    31. negative regulation of planar cell polarity pathway involved in axis elongation Source: MGI
    32. negative regulation of transcription, DNA-templated Source: Ensembl
    33. negative regulation of Wnt signaling pathway Source: BHF-UCL
    34. neural tube closure Source: MGI
    35. neural tube development Source: MGI
    36. outflow tract morphogenesis Source: Ensembl
    37. patterning of blood vessels Source: BHF-UCL
    38. planar cell polarity pathway involved in neural tube closure Source: MGI
    39. positive regulation of angiogenesis Source: BHF-UCL
    40. positive regulation of apoptotic process Source: Ensembl
    41. positive regulation of canonical Wnt signaling pathway Source: BHF-UCL
    42. positive regulation of catenin import into nucleus Source: BHF-UCL
    43. positive regulation of cell growth Source: UniProtKB
    44. positive regulation of cell proliferation Source: UniProtKB
    45. positive regulation of endopeptidase activity Source: MGI
    46. positive regulation of fat cell differentiation Source: MGI
    47. positive regulation of osteoblast differentiation Source: MGI
    48. positive regulation of peptidyl-serine phosphorylation Source: BHF-UCL
    49. positive regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
    50. post-anal tail morphogenesis Source: MGI
    51. regulation of apoptotic process Source: MGI
    52. regulation of cell proliferation Source: MGI
    53. regulation of establishment of planar polarity Source: MGI
    54. regulation of stem cell division Source: UniProtKB
    55. regulation of Wnt signaling pathway Source: UniProtKB
    56. response to drug Source: UniProtKB
    57. response to nutrient Source: Ensembl
    58. sclerotome development Source: BHF-UCL
    59. somitogenesis Source: MGI
    60. Wnt signaling pathway involved in somitogenesis Source: MGI

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Wnt signaling pathway

    Enzyme and pathway databases

    ReactomeiREACT_210612. negative regulation of TCF-dependent signaling by WNT ligand antagonists.

    Protein family/group databases

    MEROPSiI93.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Secreted frizzled-related protein 2
    Short name:
    sFRP-2
    Alternative name(s):
    Protein SDF5
    Secreted apoptosis-related protein 1
    Short name:
    SARP-1
    Gene namesi
    Name:Sfrp2
    Synonyms:Sarp1, Sdf5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:108078. Sfrp2.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: RefGenome
    2. extracellular space Source: UniProtKB
    3. plasma membrane Source: RefGenome

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Chaini25 – 295271Secreted frizzled-related protein 2PRO_0000032543Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi40 ↔ 103By similarity
    Disulfide bondi50 ↔ 96By similarity
    Disulfide bondi87 ↔ 125By similarity
    Disulfide bondi114 ↔ 152By similarity
    Disulfide bondi118 ↔ 142By similarity
    Disulfide bondi172 ↔ 245By similarity
    Disulfide bondi175 ↔ 247By similarity
    Disulfide bondi190 ↔ 295By similarity

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP97299.

    PTM databases

    PhosphoSiteiP97299.

    Expressioni

    Tissue specificityi

    Highly expressed in the eye. Weaker expression in heart and lung.1 Publication

    Developmental stagei

    During kidney development, expressed at E10.5 in the mesonephric tubules, and at E12.5 strongly expressed in the comma shaped bodies and surrounding ureter stalk. In E14.5 kidney, expressed in the S-shaped bodies. In the developing nervous system, expressed in the presumptive hindbrain and the ventral part of the neural tube from E8.0 onwards. At E9.5, expression is additionally detected in the mesonephros and the optic vesicle. E10 expression is found in the second and third branchial cleft, in the eye, the ventral neural tube and specific rhombomeres and prosomers. E10.5 brain shows specific expression in the basal and alar plate. In developing eye, expressed at E9.0 in the optic placode, and at E9.5 in the optic vesicle. By E10.5, expression found in the lens vesicle and the inner layer of the invaginating optic vesicle. Strong expression at E14.5 in the anterior lens epithelium, decreasing thereafter. Expression also found in the prospective neural retina. In developing limbs, expression found at E11.5 in the shoulder and at the distal end of the cartilaginous condensation. At E12.5, expressed in the foot and hand paddle extending along the digital rays. Expressed, at E13.5 and E14.5, in the forelimb and hindlimb where the interphalangeal joints will develop. Also expressed at E14.5 between the sternal bands and where the ribs contact the sternum. In other developing structures, expression found at E11.5, in the maxillary and mandibular component of the first branchial arch, and later, in the loose mesenchyme surrounding cartilage and epithelia of the skull as well as in the whisker follicles. Also expressed in developing teeth, with the highest levels at E15.5 and E16.5 in the mesenchyme and the dental epithelium of the developing molars. Expressed in development smooth muscle surrounding the esophagus at E11.5, the dorsal aorta and the ductus arteriosus at E14.5, and the ureter stalk at E15.5.2 Publications

    Gene expression databases

    ArrayExpressiP97299.
    BgeeiP97299.
    CleanExiMM_SFRP2.
    GenevestigatoriP97299.

    Interactioni

    Protein-protein interaction databases

    BioGridi203142. 9 interactions.
    DIPiDIP-59486N.

    Structurei

    3D structure databases

    ProteinModelPortaliP97299.
    SMRiP97299. Positions 49-150.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini35 – 155121FZPROSITE-ProRule annotationAdd
    BLAST
    Domaini172 – 295124NTRPROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The FZ domain is involved in binding with Wnt ligands.By similarity

    Sequence similaritiesi

    Contains 1 FZ (frizzled) domain.PROSITE-ProRule annotation
    Contains 1 NTR domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG240950.
    HOGENOMiHOG000231058.
    HOVERGENiHBG052928.
    InParanoidiP97299.
    KOiK02176.
    OMAiTSLKRWQ.
    OrthoDBiEOG789CBZ.
    PhylomeDBiP97299.
    TreeFamiTF350133.

    Family and domain databases

    Gene3Di1.10.2000.10. 1 hit.
    InterProiIPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR001134. Netrin_domain.
    IPR018933. Netrin_module_non-TIMP.
    IPR026558. SFRP2.
    IPR008993. TIMP-like_OB-fold.
    [Graphical view]
    PANTHERiPTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF45. PTHR11309:SF45. 1 hit.
    PfamiPF01392. Fz. 1 hit.
    PF01759. NTR. 1 hit.
    [Graphical view]
    SMARTiSM00643. C345C. 1 hit.
    SM00063. FRI. 1 hit.
    [Graphical view]
    SUPFAMiSSF50242. SSF50242. 1 hit.
    SSF63501. SSF63501. 1 hit.
    PROSITEiPS50038. FZ. 1 hit.
    PS50189. NTR. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97299-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPRGPASLLL LVLASHCCLG SARGLFLFGQ PDFSYKRSNC KPIPANLQLC    50
    HGIEYQNMRL PNLLGHETMK EVLEQAGAWI PLVMKQCHPD TKKFLCSLFA 100
    PVCLDDLDET IQPCHSLCVQ VKDRCAPVMS AFGFPWPDML ECDRFPQDND 150
    LCIPLASSDH LLPATEEAPK VCEACKTKNE DDNDIMETLC KNDFALKIKV 200
    KEITYINRDT KIILETKSKT IYKLNGVSER DLKKSVLWLK DSLQCTCEEM 250
    NDINAPYLVM GQKQGGELVI TSVKRWQKGQ REFKRISRSI RKLQC 295
    Length:295
    Mass (Da):33,469
    Last modified:March 15, 2005 - v2
    Checksum:i2D287C177C974C62
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti38 – 381S → T in AAB70795. (PubMed:9391078)Curated
    Sequence conflicti119 – 1191V → M in BAA09053. (PubMed:8938438)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50462 mRNA. Translation: BAA09053.1.
    U88567 mRNA. Translation: AAC53146.1.
    AF017989 mRNA. Translation: AAB70795.1.
    BC014722 mRNA. Translation: AAH14722.1.
    CCDSiCCDS17434.1.
    RefSeqiNP_033170.1. NM_009144.2.
    UniGeneiMm.19155.

    Genome annotation databases

    EnsembliENSMUST00000029625; ENSMUSP00000029625; ENSMUSG00000027996.
    GeneIDi20319.
    KEGGimmu:20319.
    UCSCiuc008ppl.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50462 mRNA. Translation: BAA09053.1 .
    U88567 mRNA. Translation: AAC53146.1 .
    AF017989 mRNA. Translation: AAB70795.1 .
    BC014722 mRNA. Translation: AAH14722.1 .
    CCDSi CCDS17434.1.
    RefSeqi NP_033170.1. NM_009144.2.
    UniGenei Mm.19155.

    3D structure databases

    ProteinModelPortali P97299.
    SMRi P97299. Positions 49-150.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 203142. 9 interactions.
    DIPi DIP-59486N.

    Protein family/group databases

    MEROPSi I93.001.

    PTM databases

    PhosphoSitei P97299.

    Proteomic databases

    PRIDEi P97299.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029625 ; ENSMUSP00000029625 ; ENSMUSG00000027996 .
    GeneIDi 20319.
    KEGGi mmu:20319.
    UCSCi uc008ppl.2. mouse.

    Organism-specific databases

    CTDi 6423.
    MGIi MGI:108078. Sfrp2.

    Phylogenomic databases

    eggNOGi NOG240950.
    HOGENOMi HOG000231058.
    HOVERGENi HBG052928.
    InParanoidi P97299.
    KOi K02176.
    OMAi TSLKRWQ.
    OrthoDBi EOG789CBZ.
    PhylomeDBi P97299.
    TreeFami TF350133.

    Enzyme and pathway databases

    Reactomei REACT_210612. negative regulation of TCF-dependent signaling by WNT ligand antagonists.

    Miscellaneous databases

    ChiTaRSi SFRP2. mouse.
    NextBioi 298111.
    PROi P97299.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97299.
    Bgeei P97299.
    CleanExi MM_SFRP2.
    Genevestigatori P97299.

    Family and domain databases

    Gene3Di 1.10.2000.10. 1 hit.
    InterProi IPR015526. Frizzled/SFRP.
    IPR020067. Frizzled_dom.
    IPR001134. Netrin_domain.
    IPR018933. Netrin_module_non-TIMP.
    IPR026558. SFRP2.
    IPR008993. TIMP-like_OB-fold.
    [Graphical view ]
    PANTHERi PTHR11309. PTHR11309. 1 hit.
    PTHR11309:SF45. PTHR11309:SF45. 1 hit.
    Pfami PF01392. Fz. 1 hit.
    PF01759. NTR. 1 hit.
    [Graphical view ]
    SMARTi SM00643. C345C. 1 hit.
    SM00063. FRI. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50242. SSF50242. 1 hit.
    SSF63501. SSF63501. 1 hit.
    PROSITEi PS50038. FZ. 1 hit.
    PS50189. NTR. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of novel secreted and membrane proteins isolated by the signal sequence trap method."
      Shirozu M., Tada H., Tashiro K., Nakamura T., Lopez N.D., Nazarea M., Hamada T., Sato T., Nakano T., Honjo T.
      Genomics 37:273-280(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Bone marrow stroma.
    2. "A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors."
      Rattner A., Hsieh J.-C., Smallwood P.M., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J.
      Proc. Natl. Acad. Sci. U.S.A. 94:2859-2863(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: C57BL/6J.
      Tissue: Embryonic eye.
    3. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Pancreas.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary tumor.
    5. "Developmental expression patterns of mouse sFRP genes encoding members of the secreted frizzled related protein family."
      Leimeister C., Bach A., Gessler M.
      Mech. Dev. 75:29-42(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: DEVELOPMENTAL STAGE.
    6. Cited for: DEVELOPMENTAL STAGE.

    Entry informationi

    Entry nameiSFRP2_MOUSE
    AccessioniPrimary (citable) accession number: P97299
    Secondary accession number(s): O08862, O35297
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: March 15, 2005
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3