Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P97299 (SFRP2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Secreted frizzled-related protein 2

Short name=sFRP-2
Alternative name(s):
Protein SDF5
Secreted apoptosis-related protein 1
Short name=SARP-1
Gene names
Name:Sfrp2
Synonyms:Sarp1, Sdf5
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length295 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis.

Subcellular location

Secreted.

Tissue specificity

Highly expressed in the eye. Weaker expression in heart and lung. Ref.2

Developmental stage

During kidney development, expressed at E10.5 in the mesonephric tubules, and at E12.5 strongly expressed in the comma shaped bodies and surrounding ureter stalk. In E14.5 kidney, expressed in the S-shaped bodies. In the developing nervous system, expressed in the presumptive hindbrain and the ventral part of the neural tube from E8.0 onwards. At E9.5, expression is additionally detected in the mesonephros and the optic vesicle. E10 expression is found in the second and third branchial cleft, in the eye, the ventral neural tube and specific rhombomeres and prosomers. E10.5 brain shows specific expression in the basal and alar plate. In developing eye, expressed at E9.0 in the optic placode, and at E9.5 in the optic vesicle. By E10.5, expression found in the lens vesicle and the inner layer of the invaginating optic vesicle. Strong expression at E14.5 in the anterior lens epithelium, decreasing thereafter. Expression also found in the prospective neural retina. In developing limbs, expression found at E11.5 in the shoulder and at the distal end of the cartilaginous condensation. At E12.5, expressed in the foot and hand paddle extending along the digital rays. Expressed, at E13.5 and E14.5, in the forelimb and hindlimb where the interphalangeal joints will develop. Also expressed at E14.5 between the sternal bands and where the ribs contact the sternum. In other developing structures, expression found at E11.5, in the maxillary and mandibular component of the first branchial arch, and later, in the loose mesenchyme surrounding cartilage and epithelia of the skull as well as in the whisker follicles. Also expressed in developing teeth, with the highest levels at E15.5 and E16.5 in the mesenchyme and the dental epithelium of the developing molars. Expressed in development smooth muscle surrounding the esophagus at E11.5, the dorsal aorta and the ductus arteriosus at E14.5, and the ureter stalk at E15.5. Ref.5 Ref.6

Domain

The FZ domain is involved in binding with Wnt ligands By similarity.

Sequence similarities

Belongs to the secreted frizzled-related protein (sFRP) family.

Contains 1 FZ (frizzled) domain.

Contains 1 NTR domain.

Ontologies

Keywords
   Biological processDifferentiation
Wnt signaling pathway
   Cellular componentSecreted
   DomainSignal
   Molecular functionDevelopmental protein
   PTMDisulfide bond
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processWnt signaling pathway involved in somitogenesis

Inferred from genetic interaction PubMed 18257070. Source: MGI

anterior/posterior pattern specification

Inferred from genetic interaction PubMed 16467359. Source: MGI

apoptotic process

Inferred from mutant phenotype PubMed 18729207. Source: MGI

bone morphogenesis

Inferred from mutant phenotype PubMed 9038166. Source: MGI

brain development

Inferred from Biological aspect of Ancestor. Source: RefGenome

cardiac left ventricle morphogenesis

Inferred from electronic annotation. Source: Ensembl

cartilage development

Inferred from mutant phenotype PubMed 18729207. Source: MGI

cell death

Inferred from mutant phenotype. Source: MGI

cellular response to X-ray

Inferred from direct assay PubMed 19778523. Source: UniProtKB

cellular response to extracellular stimulus

Inferred from direct assay PubMed 10322635. Source: MGI

chondrocyte development

Inferred from mutant phenotype PubMed 18729207. Source: MGI

collagen fibril organization

Inferred from mutant phenotype PubMed 19079247. Source: MGI

convergent extension involved in axis elongation

Inferred from genetic interaction PubMed 18257070. Source: MGI

development of primary male sexual characteristics

Inferred from genetic interaction PubMed 19100252. Source: MGI

digestive tract morphogenesis

Inferred from genetic interaction PubMed 19300477. Source: MGI

embryonic digit morphogenesis

Inferred from mutant phenotype PubMed 18729207. Source: MGI

hematopoietic stem cell proliferation

Inferred from direct assay PubMed 19778523. Source: UniProtKB

male gonad development

Inferred from genetic interaction PubMed 19100252. Source: MGI

negative regulation of BMP signaling pathway

Inferred from direct assay PubMed 19850029. Source: MGI

negative regulation of JUN kinase activity

Inferred from genetic interaction PubMed 19300477. Source: MGI

negative regulation of Wnt signaling pathway

Inferred from direct assay PubMed 10654605. Source: BHF-UCL

negative regulation of canonical Wnt signaling pathway

Inferred from direct assay PubMed 17804636. Source: MGI

negative regulation of cardiac muscle cell apoptotic process

Inferred from direct assay PubMed 17251350. Source: MGI

negative regulation of cell growth

Inferred from electronic annotation. Source: Ensembl

negative regulation of cell migration

Inferred from direct assay PubMed 10980594. Source: UniProtKB

negative regulation of cysteine-type endopeptidase activity involved in apoptotic process

Inferred from direct assay PubMed 17251350. Source: MGI

negative regulation of dermatome development

Inferred from direct assay PubMed 10654605. Source: BHF-UCL

negative regulation of epithelial cell proliferation

Inferred from electronic annotation. Source: Ensembl

negative regulation of epithelial to mesenchymal transition

Inferred from electronic annotation. Source: Ensembl

negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

Inferred from direct assay Ref.3. Source: BHF-UCL

negative regulation of gene expression

Inferred from direct assay PubMed 10980594. Source: UniProtKB

negative regulation of mesodermal cell fate specification

Inferred from genetic interaction PubMed 17462603. Source: MGI

negative regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 10980594. Source: UniProtKB

negative regulation of planar cell polarity pathway involved in axis elongation

Inferred from genetic interaction PubMed 18257070. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from electronic annotation. Source: Ensembl

neural tube closure

Inferred from genetic interaction PubMed 19850029. Source: MGI

neural tube development

Inferred from genetic interaction PubMed 19850029. Source: MGI

outflow tract morphogenesis

Inferred from electronic annotation. Source: Ensembl

patterning of blood vessels

Inferred from direct assay PubMed 19458075. Source: BHF-UCL

planar cell polarity pathway involved in neural tube closure

Inferred from genetic interaction PubMed 18257070. Source: MGI

positive regulation of angiogenesis

Inferred from direct assay PubMed 19458075. Source: BHF-UCL

positive regulation of apoptotic process

Inferred from electronic annotation. Source: Ensembl

positive regulation of canonical Wnt signaling pathway

Inferred from direct assay PubMed 20723538. Source: BHF-UCL

positive regulation of catenin import into nucleus

Inferred from direct assay PubMed 20723538. Source: BHF-UCL

positive regulation of cell growth

Inferred from direct assay PubMed 10980594. Source: UniProtKB

positive regulation of cell proliferation

Inferred from direct assay PubMed 10980594. Source: UniProtKB

positive regulation of endopeptidase activity

Inferred from direct assay PubMed 19079247. Source: MGI

positive regulation of fat cell differentiation

Inferred from sequence orthology PubMed 12055200. Source: MGI

positive regulation of osteoblast differentiation

Inferred from genetic interaction PubMed 18166153. Source: MGI

positive regulation of peptidyl-serine phosphorylation

Inferred from direct assay PubMed 20723538. Source: BHF-UCL

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 20723538. Source: BHF-UCL

post-anal tail morphogenesis

Inferred from mutant phenotype PubMed 18729207. Source: MGI

regulation of Wnt signaling pathway

Inferred from direct assay Ref.3. Source: UniProtKB

regulation of apoptotic process

Inferred from mutant phenotype PubMed 18729207. Source: MGI

regulation of cell proliferation

Inferred from mutant phenotype PubMed 18729207. Source: MGI

regulation of establishment of planar polarity

Inferred from genetic interaction PubMed 19300477. Source: MGI

regulation of stem cell division

Inferred from direct assay PubMed 19778523. Source: UniProtKB

response to drug

Inferred from direct assay PubMed 19778523. Source: UniProtKB

response to nutrient

Inferred from electronic annotation. Source: Ensembl

sclerotome development

Inferred from expression pattern PubMed 10654605. Source: BHF-UCL

somitogenesis

Inferred from genetic interaction PubMed 16467359. Source: MGI

   Cellular_componentcytoplasm

Inferred from Biological aspect of Ancestor. Source: RefGenome

extracellular space

Inferred from direct assay PubMed 10980594Ref.3. Source: UniProtKB

plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionPDZ domain binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-activated receptor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Wnt-protein binding

Inferred from physical interaction PubMed 17462603. Source: MGI

endopeptidase activator activity

Inferred from direct assay PubMed 19079247. Source: MGI

metalloenzyme activator activity

Inferred from direct assay PubMed 19079247. Source: MGI

protein binding

Inferred from physical interaction PubMed 19079247Ref.2PubMed 9853965. Source: MGI

receptor agonist activity

Inferred from direct assay PubMed 20723538. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 295271Secreted frizzled-related protein 2
PRO_0000032543

Regions

Domain35 – 155121FZ
Domain172 – 295124NTR

Amino acid modifications

Disulfide bond40 ↔ 103 By similarity
Disulfide bond50 ↔ 96 By similarity
Disulfide bond87 ↔ 125 By similarity
Disulfide bond114 ↔ 152 By similarity
Disulfide bond118 ↔ 142 By similarity
Disulfide bond172 ↔ 245 By similarity
Disulfide bond175 ↔ 247 By similarity
Disulfide bond190 ↔ 295 By similarity

Experimental info

Sequence conflict381S → T in AAB70795. Ref.3
Sequence conflict1191V → M in BAA09053. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P97299 [UniParc].

Last modified March 15, 2005. Version 2.
Checksum: 2D287C177C974C62

FASTA29533,469
        10         20         30         40         50         60 
MPRGPASLLL LVLASHCCLG SARGLFLFGQ PDFSYKRSNC KPIPANLQLC HGIEYQNMRL 

        70         80         90        100        110        120 
PNLLGHETMK EVLEQAGAWI PLVMKQCHPD TKKFLCSLFA PVCLDDLDET IQPCHSLCVQ 

       130        140        150        160        170        180 
VKDRCAPVMS AFGFPWPDML ECDRFPQDND LCIPLASSDH LLPATEEAPK VCEACKTKNE 

       190        200        210        220        230        240 
DDNDIMETLC KNDFALKIKV KEITYINRDT KIILETKSKT IYKLNGVSER DLKKSVLWLK 

       250        260        270        280        290 
DSLQCTCEEM NDINAPYLVM GQKQGGELVI TSVKRWQKGQ REFKRISRSI RKLQC 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of novel secreted and membrane proteins isolated by the signal sequence trap method."
Shirozu M., Tada H., Tashiro K., Nakamura T., Lopez N.D., Nazarea M., Hamada T., Sato T., Nakano T., Honjo T.
Genomics 37:273-280(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Bone marrow stroma.
[2]"A family of secreted proteins contains homology to the cysteine-rich ligand-binding domain of frizzled receptors."
Rattner A., Hsieh J.-C., Smallwood P.M., Gilbert D.J., Copeland N.G., Jenkins N.A., Nathans J.
Proc. Natl. Acad. Sci. U.S.A. 94:2859-2863(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Strain: C57BL/6J.
Tissue: Embryonic eye.
[3]"SARPs: a family of secreted apoptosis-related proteins."
Melkonyan H.S., Chang W.C., Shapiro J.P., Mahadevappa M., Fitzpatrick P.A., Kiefer M.C., Tomei L.D., Umansky S.R.
Proc. Natl. Acad. Sci. U.S.A. 94:13636-13641(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pancreas.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: FVB/N.
Tissue: Mammary tumor.
[5]"Developmental expression patterns of mouse sFRP genes encoding members of the secreted frizzled related protein family."
Leimeister C., Bach A., Gessler M.
Mech. Dev. 75:29-42(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
[6]"Genomic analysis of mouse retinal development."
Blackshaw S., Harpavat S., Trimarchi J., Cai L., Huang H., Kuo W.P., Weber G., Lee K., Fraioli R.E., Cho S.-H., Yung R., Asch E., Ohno-Machado L., Wong W.H., Cepko C.L.
PLoS Biol. 2:1411-1431(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: DEVELOPMENTAL STAGE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D50462 mRNA. Translation: BAA09053.1.
U88567 mRNA. Translation: AAC53146.1.
AF017989 mRNA. Translation: AAB70795.1.
BC014722 mRNA. Translation: AAH14722.1.
CCDSCCDS17434.1.
RefSeqNP_033170.1. NM_009144.2.
UniGeneMm.19155.

3D structure databases

ProteinModelPortalP97299.
SMRP97299. Positions 49-150.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203142. 9 interactions.
DIPDIP-59486N.

Protein family/group databases

MEROPSI93.001.

PTM databases

PhosphoSiteP97299.

Proteomic databases

PRIDEP97299.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000029625; ENSMUSP00000029625; ENSMUSG00000027996.
GeneID20319.
KEGGmmu:20319.
UCSCuc008ppl.2. mouse.

Organism-specific databases

CTD6423.
MGIMGI:108078. Sfrp2.

Phylogenomic databases

eggNOGNOG240950.
HOGENOMHOG000231058.
HOVERGENHBG052928.
InParanoidP97299.
KOK02176.
OMATSLKRWQ.
OrthoDBEOG789CBZ.
PhylomeDBP97299.
TreeFamTF350133.

Gene expression databases

ArrayExpressP97299.
BgeeP97299.
CleanExMM_SFRP2.
GenevestigatorP97299.

Family and domain databases

Gene3D1.10.2000.10. 1 hit.
InterProIPR015526. Frizzled/SFRP.
IPR020067. Frizzled_dom.
IPR001134. Netrin_domain.
IPR018933. Netrin_module_non-TIMP.
IPR026558. SFRP2.
IPR008993. TIMP-like_OB-fold.
[Graphical view]
PANTHERPTHR11309. PTHR11309. 1 hit.
PTHR11309:SF45. PTHR11309:SF45. 1 hit.
PfamPF01392. Fz. 1 hit.
PF01759. NTR. 1 hit.
[Graphical view]
SMARTSM00643. C345C. 1 hit.
SM00063. FRI. 1 hit.
[Graphical view]
SUPFAMSSF50242. SSF50242. 1 hit.
SSF63501. SSF63501. 1 hit.
PROSITEPS50038. FZ. 1 hit.
PS50189. NTR. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSFRP2. mouse.
NextBio298111.
PROP97299.
SOURCESearch...

Entry information

Entry nameSFRP2_MOUSE
AccessionPrimary (citable) accession number: P97299
Secondary accession number(s): O08862, O35297
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot