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P97298

- PEDF_MOUSE

UniProt

P97298 - PEDF_MOUSE

Protein

Pigment epithelium-derived factor

Gene

Serpinf1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 2 (29 Mar 2004)
      Previous versions | rss
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    Functioni

    Neurotrophic protein; induces extensive neuronal differentiation in retinoblastoma cells. Potent inhibitor of angiogenesis. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.1 Publication

    GO - Molecular functioni

    1. serine-type endopeptidase inhibitor activity Source: RefGenome

    GO - Biological processi

    1. aging Source: Ensembl
    2. kidney development Source: Ensembl
    3. negative regulation of angiogenesis Source: UniProtKB
    4. negative regulation of endopeptidase activity Source: RefGenome
    5. negative regulation of epithelial cell proliferation involved in prostate gland development Source: MGI
    6. negative regulation of inflammatory response Source: Ensembl
    7. positive regulation of neurogenesis Source: UniProtKB
    8. regulation of proteolysis Source: RefGenome
    9. response to glucocorticoid Source: Ensembl
    10. response to retinoic acid Source: Ensembl
    11. short-term memory Source: Ensembl

    Protein family/group databases

    MEROPSiI04.979.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Pigment epithelium-derived factor
    Short name:
    PEDF
    Alternative name(s):
    Caspin
    Serpin F1
    Stromal cell-derived factor 3
    Short name:
    SDF-3
    Gene namesi
    Name:Serpinf1
    Synonyms:Pedf, Sdf3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:108080. Serpinf1.

    Subcellular locationi

    Secreted. Melanosome By similarity

    GO - Cellular componenti

    1. extracellular matrix Source: Ensembl
    2. extracellular region Source: UniProtKB
    3. extracellular space Source: MGI
    4. melanosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919By similarityAdd
    BLAST
    Chaini20 – 417398Pigment epithelium-derived factorPRO_0000032509Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei20 – 201Pyrrolidone carboxylic acidBy similarity
    Modified residuei24 – 241PhosphoserineBy similarity
    Glycosylationi284 – 2841N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Phosphoprotein, Pyrrolidone carboxylic acid

    Proteomic databases

    MaxQBiP97298.
    PaxDbiP97298.
    PRIDEiP97298.

    PTM databases

    PhosphoSiteiP97298.

    Expressioni

    Tissue specificityi

    Highly expressed in the liver, gastric glandular mucosa and renal tubules. It is also expressed in the brain, heart, lung retina and testes.2 Publications

    Developmental stagei

    First detected at 12.5 dpc in cartilage primordium, it is present in the osseous matrix of developing limbs, vertebrae, ribs and skull. At 16.5 dpc it is detected in bone matrix and smooth muscle, and at lower levels in connective tissue, bronchial epithelial cells, metanephron microtubules, and skin.1 Publication

    Gene expression databases

    ArrayExpressiP97298.
    BgeeiP97298.
    GenevestigatoriP97298.

    Structurei

    3D structure databases

    ProteinModelPortaliP97298.
    SMRiP97298. Positions 35-414.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the serpin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4826.
    GeneTreeiENSGT00750000117478.
    HOGENOMiHOG000115489.
    HOVERGENiHBG106911.
    InParanoidiP97298.
    OMAiLNCKIAQ.
    OrthoDBiEOG7K3TMD.
    PhylomeDBiP97298.
    TreeFamiTF317350.

    Family and domain databases

    InterProiIPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view]
    PANTHERiPTHR11461. PTHR11461. 1 hit.
    PfamiPF00079. Serpin. 1 hit.
    [Graphical view]
    SMARTiSM00093. SERPIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF56574. SSF56574. 1 hit.
    PROSITEiPS00284. SERPIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P97298-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQALVLLLWT GALLGHGSSQ NVPSSSEGSP VPDSTGEPVE EEDPFFKVPV    50
    NKLAAAVSNF GYDLYRLRSS ASPTGNVLLS PLSVATALSA LSLGAEHRTE 100
    SVIHRALYYD LITNPDIHST YKELLASVTA PEKNLKSASR IVFERKLRVK 150
    SSFVAPLEKS YGTRPRILTG NPRVDLQEIN NWVQAQMKGK IARSTREMPS 200
    ALSILLLGVA YFKGQWVTKF DSRKTTLQDF HLDEDRTVRV PMMSDPKAIL 250
    RYGLDSDLNC KIAQLPLTGS MSIIFFLPLT VTQNLTMIEE SLTSEFIHDI 300
    DRELKTIQAV LTVPKLKLSF EGELTKSLQD MKLQSLFESP DFSKITGKPV 350
    KLTQVEHRAA FEWNEEGAGS SPSPGLQPVR LTFPLDYHLN QPFLFVLRDT 400
    DTGALLFIGR ILDPSST 417
    Length:417
    Mass (Da):46,234
    Last modified:March 29, 2004 - v2
    Checksum:iECD360FE6AA74D25
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti70 – 701S → G in AAC69271. 1 PublicationCurated
    Sequence conflicti136 – 1361K → N in BAA31978. (PubMed:9614124)Curated
    Sequence conflicti137 – 1371S → R in AAC69271. 1 PublicationCurated
    Sequence conflicti280 – 2801T → A in BAA09051. (PubMed:8938438)Curated
    Sequence conflicti377 – 3771Q → L in AAC69271. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50460 mRNA. Translation: BAA09051.1.
    AF036164 mRNA. Translation: AAC69271.1.
    D87975 mRNA. Translation: BAA31978.1.
    AF017055
    , AF017051, AF017052, AF017053, AF017054 Genomic DNA. Translation: AAC05733.1.
    AF017057 mRNA. Translation: AAC05731.1.
    BC019852 mRNA. Translation: AAH19852.1.
    CCDSiCCDS25045.1.
    RefSeqiNP_035470.3. NM_011340.3.
    UniGeneiMm.2044.

    Genome annotation databases

    EnsembliENSMUST00000000769; ENSMUSP00000000769; ENSMUSG00000000753.
    GeneIDi20317.
    KEGGimmu:20317.
    UCSCiuc007kdp.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D50460 mRNA. Translation: BAA09051.1 .
    AF036164 mRNA. Translation: AAC69271.1 .
    D87975 mRNA. Translation: BAA31978.1 .
    AF017055
    , AF017051 , AF017052 , AF017053 , AF017054 Genomic DNA. Translation: AAC05733.1 .
    AF017057 mRNA. Translation: AAC05731.1 .
    BC019852 mRNA. Translation: AAH19852.1 .
    CCDSi CCDS25045.1.
    RefSeqi NP_035470.3. NM_011340.3.
    UniGenei Mm.2044.

    3D structure databases

    ProteinModelPortali P97298.
    SMRi P97298. Positions 35-414.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi I04.979.

    PTM databases

    PhosphoSitei P97298.

    Proteomic databases

    MaxQBi P97298.
    PaxDbi P97298.
    PRIDEi P97298.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000769 ; ENSMUSP00000000769 ; ENSMUSG00000000753 .
    GeneIDi 20317.
    KEGGi mmu:20317.
    UCSCi uc007kdp.1. mouse.

    Organism-specific databases

    CTDi 5176.
    MGIi MGI:108080. Serpinf1.

    Phylogenomic databases

    eggNOGi COG4826.
    GeneTreei ENSGT00750000117478.
    HOGENOMi HOG000115489.
    HOVERGENi HBG106911.
    InParanoidi P97298.
    OMAi LNCKIAQ.
    OrthoDBi EOG7K3TMD.
    PhylomeDBi P97298.
    TreeFami TF317350.

    Miscellaneous databases

    ChiTaRSi SERPINF1. mouse.
    NextBioi 298103.
    PROi P97298.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P97298.
    Bgeei P97298.
    Genevestigatori P97298.

    Family and domain databases

    InterProi IPR023795. Serpin_CS.
    IPR023796. Serpin_dom.
    IPR000215. Serpin_fam.
    [Graphical view ]
    PANTHERi PTHR11461. PTHR11461. 1 hit.
    Pfami PF00079. Serpin. 1 hit.
    [Graphical view ]
    SMARTi SM00093. SERPIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56574. SSF56574. 1 hit.
    PROSITEi PS00284. SERPIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of novel secreted and membrane proteins isolated by the signal sequence trap method."
      Shirozu M., Tada H., Tashiro K., Nakamura T., Lopez N.D., Nazarea M., Hamada T., Sato T., Nakano T., Honjo T.
      Genomics 37:273-280(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Isolation, purification and characterization of a collagen-associated serpin, caspin, produced by murine colon adenocarcinoma cells."
      Kozaki K., Miyaishi O., Koiwai O., Yasui Y., Kashiwai A., Nishikawa Y., Shimizu S., Saga S.
      J. Biol. Chem. 273:15125-15130(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 53-66; 252-261; 333-344 AND 359-372, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Strain: BALB/c.
      Tissue: Liver.
    3. "Structural and comparative analysis of the mouse gene for pigment epithelium-derived factor (PEDF)."
      Singh V.K., Chader G.J., Rodriguez I.R.
      Mol. Vis. 4:7-7(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
      Strain: FVB/N.
      Tissue: Liver.
    4. Tombran-Tink J.
      Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: BALB/c.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    6. "Wilms' tumor growth is suppressed by antiangiogenic pigment epithelium-derived factor in a xenograft model."
      Abramson L.P., Stellmach V., Doll J.A., Cornwell M., Arensman R.M., Crawford S.E.
      J. Pediatr. Surg. 38:336-342(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPEDF_MOUSE
    AccessioniPrimary (citable) accession number: P97298
    Secondary accession number(s): O70629, O88691
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: March 29, 2004
    Last modified: October 1, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3