P97297 (ADML_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ADM

Cleaved into the following 2 chains:

1. Adrenomedullin
   Short name=AM
2. Proadrenomedullin N-20 terminal peptide
   Alternative name(s):
   ProAM N-terminal 20 peptide
   Short name=PAMP
   Short name=ProAM-N20

Gene names

Name:

Adm

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	184 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	AM and PAMP are potent hypotensive and vasodilatator agents.
Subcellular location	Secreted.
Sequence similarities	Belongs to the adrenomedullin family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hormone
PTM	Amidation Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	G-protein coupled receptor internalization Inferred from electronic annotation. Source: Compara aging Inferred from electronic annotation. Source: Compara androgen metabolic process Inferred from electronic annotation. Source: Compara branching involved in labyrinthine layer morphogenesis Inferred from mutant phenotype PubMed 16981008. Source: MGI cAMP biosynthetic process Inferred from electronic annotation. Source: Compara cAMP-mediated signaling Inferred from electronic annotation. Source: Compara developmental growth Inferred from mutant phenotype PubMed 16981008. Source: MGI elevation of cytosolic calcium ion concentration Inferred from electronic annotation. Source: Compara female pregnancy Inferred from electronic annotation. Source: Compara heart development Inferred from mutant phenotype PubMed 18097473. Source: UniProtKB hormone secretion Inferred from electronic annotation. Source: Compara negative regulation of cell proliferation Inferred from electronic annotation. Source: Compara negative regulation of vascular permeability Inferred from mutant phenotype PubMed 18097473. Source: UniProtKB negative regulation of vasoconstriction Inferred from electronic annotation. Source: Compara neural tube closure Inferred from mutant phenotype PubMed 16981008. Source: MGI neuron projection regeneration Inferred from electronic annotation. Source: Compara odontogenesis of dentin-containing tooth Inferred from electronic annotation. Source: Compara organ regeneration Inferred from electronic annotation. Source: Compara positive regulation of angiogenesis Inferred from mutant phenotype PubMed 18097473. Source: UniProtKB positive regulation of cAMP biosynthetic process Inferred from electronic annotation. Source: Compara positive regulation of cell proliferation Inferred from mutant phenotype PubMed 16537897. Source: MGI positive regulation of heart rate Inferred from electronic annotation. Source: Compara positive regulation of vasculogenesis Inferred from mutant phenotype PubMed 18097473. Source: UniProtKB positive regulation of vasodilation Inferred from electronic annotation. Source: Compara regulation of the force of heart contraction Inferred from electronic annotation. Source: Compara response to cold Inferred from electronic annotation. Source: Compara response to glucocorticoid stimulus Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara response to insulin stimulus Inferred from electronic annotation. Source: Compara response to lipopolysaccharide Inferred from electronic annotation. Source: Compara response to starvation Inferred from electronic annotation. Source: Compara response to wounding Inferred from electronic annotation. Source: Compara spongiotrophoblast layer development Inferred from mutant phenotype PubMed 16981008. Source: MGI vascular smooth muscle cell development Inferred from mutant phenotype PubMed 18097473. Source: UniProtKB vasculogenesis Inferred from electronic annotation. Source: Compara
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara extracellular space Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	By similarity
	Peptide	22 – 41	20	Proadrenomedullin N-20 terminal peptide		PRO_0000000965
	Propeptide	45 – 92	48	By similarity		PRO_0000000966
	Peptide	95 – 144	50	Adrenomedullin		PRO_0000000967
	Propeptide	151 – 184	34	PreproAM C-terminal fragment By similarity		PRO_0000000968
Amino acid modifications
	Modified residue	41	1	Arginine amide By similarity
	Modified residue	144	1	Tyrosine amide By similarity
	Disulfide bond	108 ↔ 113		By similarity
Experimental info
	Sequence conflict	173	1	G → A in BAA11367. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P97297 [UniParc]. Last modified June 28, 2011. Version 2. Checksum: C88C9903C479C898	FASTA	184	20,750
	10 20 30 40 50 60 MKLVSITLML LGSLAFLGAD TAGPDTPSQF RKKWNKWALS RGKRELQASS SYPTGLADET 70 80 90 100 110 120 TVPTQTLDPF LDEQNTTGPL QASNQSEAHI RVKRYRQSMN QGSRSNGCRF GTCTFQKLAH 130 140 150 160 170 180 QIYQLTDKDK DGMAPRNKIS PQGYGRRRRR SLLEVLRSRT VESSQEQTHT APGPWAHISR LFRI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organization, expression, and chromosomal mapping of the mouse adrenomedullin gene." Okazaki T., Ogawa Y., Tamura N., Mori Y., Isse N., Aoki T., Rochelle J.M., Taketo M.M., Seldin M.F., Nakao K. Genomics 37:395-399(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Sv.
[2]	"Expression of adrenomedullin, a hypotensive peptide, in the trophoblast giant cells at the embryo implantation site in mouse." Yotsumoto S., Shimada T., Cui C.Y., Nakashima H., Fujiwara H., Ko M.S.H. Dev. Biol. 203:264-275(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: NOD. Tissue: Kidney and Lung.
[4]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Egg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D78349 Genomic DNA. Translation: BAA11367.1. U77630 mRNA. Translation: AAB36535.1. AK144181 mRNA. Translation: BAE25751.1. AK144600 mRNA. Translation: BAE25959.1. AK144649 mRNA. Translation: BAE25989.1. AK171066 mRNA. Translation: BAE42225.1. CH466531 Genomic DNA. Translation: EDL16987.1. BC052665 mRNA. Translation: AAH52665.1.
IPI	IPI00122995.
RefSeq	NP_033757.1. NM_009627.1.
UniGene	Mm.1408.
3D structure databases
ProteinModelPortal	P97297.
SMR	P97297. Positions 95-144.
ModBase	Search...
Protein-protein interaction databases
STRING	10090.ENSMUSP00000033054.
PTM databases
PhosphoSite	P97297.
Protocols and materials databases
DNASU	11535.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000033054; ENSMUSP00000033054; ENSMUSG00000030790.
GeneID	11535.
KEGG	mmu:11535.
Organism-specific databases
CTD	133.
MGI	MGI:108058. Adm.
Phylogenomic databases
eggNOG	NOG46109.
GeneTree	ENSGT00390000000451.
HOGENOM	HOG000033821.
HOVERGEN	HBG004181.
InParanoid	Q6GTK2.
KO	K12333.
OMA	PQDVKGA.
OrthoDB	EOG4894NP.
Gene expression databases
Bgee	P97297.
CleanEx	MM_ADM.
Genevestigator	P97297.
GermOnline	ENSMUSG00000030790. Mus musculus.
Family and domain databases
InterPro	IPR001710. Adrenomedullin. IPR021116. Procalcitonin/adrenomedullin. [Graphical view]
Pfam	PF00214. Calc_CGRP_IAPP. 1 hit. [Graphical view]
PRINTS	PR00801. ADRENOMEDULN.
ProtoNet	Search...
Other
NextBio	278978.
SOURCE	Search...

Entry information

Entry name

ADML_MOUSE

Accession

Primary (citable) accession number: P97297
Secondary accession number(s): P97453, Q6GTK2

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 28, 2011
Last modified:	April 3, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program