Reviewed, UniProtKB/Swiss-Prot P97292 (HRH2_MOUSE)

Names and origin

Protein names

Recommended name:
    Histamine H2 receptor
      Short name=H2R
Alternative name(s):
    Gastric receptor I

Gene names

Name:

Hrh2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	358 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	The H2 subclass of histamine receptors mediates gastric acid secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Subcellular location	Cell membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the G-protein coupled receptor 1 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	G-protein coupled receptor Receptor Transducer
PTM	Disulfide bond Glycoprotein Lipoprotein Palmitate
Gene Ontology (GO)
Biological process	G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histamine receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 358	358	Histamine H2 receptor		PRO_0000069685
Regions
	Topological domain	1 – 22	22	Extracellular Potential
	Transmembrane	23 – 44	22	1 Potential
	Topological domain	45 – 57	13	Cytoplasmic Potential
	Transmembrane	58 – 81	24	2 Potential
	Topological domain	82 – 92	11	Extracellular Potential
	Transmembrane	93 – 114	22	3 Potential
	Topological domain	115 – 134	20	Cytoplasmic Potential
	Transmembrane	135 – 159	25	4 Potential
	Topological domain	160 – 179	20	Extracellular Potential
	Transmembrane	180 – 203	24	5 Potential
	Topological domain	204 – 233	30	Cytoplasmic Potential
	Transmembrane	234 – 257	24	6 Potential
	Topological domain	258 – 266	9	Extracellular Potential
	Transmembrane	267 – 288	22	7 Potential
	Topological domain	289 – 358	70	Cytoplasmic Potential
Sites
	Site	98	1	Essential for histamine binding By similarity
	Site	185	1	Essential for tiotidine binding and implicated in histamine binding By similarity
	Site	189	1	Implicated in histamine binding By similarity
Amino acid modifications
	Lipidation	304	1	S-palmitoyl cysteine By similarity
	Glycosylation	4	1	N-linked (GlcNAc...) Potential
	Disulfide bond	91 ↔ 173		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97292-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: D3DBA81D71B6D927	FASTA	358	40,379
	10 20 30 40 50 60 MEPNGTVHSC CLDSIALKVT ISVVLTTLIF ITVAGNVVVC LAVSLNRRLR SLTNCFIVSL 70 80 90 100 110 120 AATDLLLGLL VMPFSAIYQL SFKWRFGQVF CNIYTSLDVM LCTASILNLF MISLDRYCAV 130 140 150 160 170 180 TDPLRYPVLV TPVRVAISLV FIWVISITLS FLSIHLGWNS RNGTRGGNDT FKCKVQVNEV 190 200 210 220 230 240 YGLVDGMVTF YLPLLIMCVT YYRIFKIARE QAKRINHISS WKAATIREHK ATVTLAAVMG 250 260 270 280 290 300 AFIVCWFPYF TAFVYRGLRG DDPVNEVVEG IVLWLGYANS ALNPILYATL NRDFRMAYQQ 310 320 330 340 350 LFHCKLASHN SHKTSLRLNN SLLSRSQSRE GRWQEEKPLK LQVWSGTELT HPQGSPVR

References

[1]	"Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene." Kobayashi T., Inoue I., Jenkins N.A., Gilbert D.J., Copeland N.G., Watanabe T. Genomics 37:390-394(1996) [PubMed: 8938453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 129/Ola.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D50096 Genomic DNA. Translation: BAA08792.1.
IPI	IPI00467449.
UniGene	Mm.57243
3D structure databases
ModBase	Search...
Protein family/group databases
GPCRDB	Search...
Genome annotation databases
Ensembl	ENSMUSG00000034987. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:108482. Hrh2.
Phylogenomic databases
HOVERGEN	P97292.
Gene expression databases
ArrayExpress	P97292.
Bgee	P97292.
CleanEx	MM_HRH2.
GermOnline	ENSMUSG00000034987. Mus musculus.
Family and domain databases
InterPro	IPR000276. 7TM_GPCR_Rhodpsn. IPR017452. GPCR_Rhodpsn_supfam. IPR000503. Histamine_H2_recept. [Graphical view]
Pfam	PF00001. 7tm_1. 1 hit. [Graphical view]
PRINTS	PR00237. GPCRRHODOPSN. PR00531. HISTAMINEH2R.
PROSITE	PS00237. G_PROTEIN_RECEP_F1_1. 1 hit. PS50262. G_PROTEIN_RECEP_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

HRH2_MOUSE

Accession

Primary (citable) accession number: P97292

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	May 1, 1997
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)