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P97288

- 5HT4R_MOUSE

UniProt

P97288 - 5HT4R_MOUSE

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Protein

5-hydroxytryptamine receptor 4

Gene

Htr4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase (By similarity).By similarity

GO - Molecular functioni

  1. serotonin receptor activity Source: Ensembl

GO - Biological processi

  1. positive regulation of cell proliferation Source: Ensembl
  2. regulation of appetite Source: InterPro
  3. synaptic transmission Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Names & Taxonomyi

Protein namesi
Recommended name:
5-hydroxytryptamine receptor 4
Short name:
5-HT-4
Short name:
5-HT4
Alternative name(s):
Serotonin receptor 4
Gene namesi
Name:Htr4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 18

Organism-specific databases

MGIiMGI:109246. Htr4.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Endosome
Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.

GO - Cellular componenti

  1. endosome Source: UniProtKB-KW
  2. integral component of plasma membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3883885-hydroxytryptamine receptor 4PRO_0000068966Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi7 – 71N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi93 ↔ 184PROSITE-ProRule annotation
Lipidationi329 – 3291S-palmitoyl cysteineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PRIDEiP97288.

PTM databases

PhosphoSiteiP97288.

Expressioni

Gene expression databases

BgeeiP97288.
CleanExiMM_HTR4.
GenevestigatoriP97288.

Interactioni

Subunit structurei

Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with INADL, NOS1 and SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR. Interacts (via C-terminus 330-346 AA) with GRK5; this interaction is promoted by 5-HT (serotonin) (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
GRK5P349478EBI-7149283,EBI-7149314From a different organism.
SRCP129312EBI-7149283,EBI-621482From a different organism.

Protein-protein interaction databases

IntActiP97288. 5 interactions.

Structurei

3D structure databases

ProteinModelPortaliP97288.
SMRiP97288. Positions 25-328.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1919ExtracellularBy similarityAdd
BLAST
Topological domaini41 – 5818CytoplasmicBy similarityAdd
BLAST
Topological domaini80 – 9314ExtracellularBy similarityAdd
BLAST
Topological domaini117 – 13721CytoplasmicBy similarityAdd
BLAST
Topological domaini159 – 19234ExtracellularBy similarityAdd
BLAST
Topological domaini214 – 26047CytoplasmicBy similarityAdd
BLAST
Topological domaini282 – 29413ExtracellularBy similarityAdd
BLAST
Topological domaini316 – 38873CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei20 – 4021Helical; Name=1By similarityAdd
BLAST
Transmembranei59 – 7921Helical; Name=2By similarityAdd
BLAST
Transmembranei94 – 11623Helical; Name=3By similarityAdd
BLAST
Transmembranei138 – 15821Helical; Name=4By similarityAdd
BLAST
Transmembranei193 – 21321Helical; Name=5By similarityAdd
BLAST
Transmembranei261 – 28121Helical; Name=6By similarityAdd
BLAST
Transmembranei295 – 31521Helical; Name=7By similarityAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG262978.
GeneTreeiENSGT00760000118774.
HOGENOMiHOG000239242.
HOVERGENiHBG106962.
InParanoidiP97288.
KOiK04160.
OMAiYRIYITA.
OrthoDBiEOG7CG707.
TreeFamiTF316350.

Family and domain databases

Gene3Di1.20.1070.10. 1 hit.
InterProiIPR001520. 5HT4_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERiPTHR24249:SF33. PTHR24249:SF33. 1 hit.
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR01059. 5HT4RECEPTR.
PR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P97288-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKLDANVSS NEGFRSVEKV VLLTFLAVVI LMAILGNLLV MVAVCRDRQL
60 70 80 90 100
RKIKTNYFIV SLAFADLLVS VLVMPFGAIE LVQDIWAYGE MFCLVRTSLD
110 120 130 140 150
VLLTTASIFH LCCISLDRYY AICCQPLVYR NKMTPLRIAL MLGGCWVLPM
160 170 180 190 200
FISFLPIMQG WNNIGIVDVI EKRKFSHNSN STWCVFMVNK PYAITCSVVA
210 220 230 240 250
FYIPFLLMVL AYYRIYVTAK EHAQQIQMLQ RAGATSESRP QPADQHSTHR
260 270 280 290 300
MRTETKAAKT LCVIMGCFCF CWAPFFVTNI VDPFIDYTVP EQVWTAFLWL
310 320 330 340 350
GYINSGLNPF LYAFLNKSFR RAFLIILCCD DERYKRPPIL GQTVPCSTTT
360 370 380
INGSTHVLRD TVECGGQWES RCHLTATSPL VAAQPSDT
Length:388
Mass (Da):43,962
Last modified:July 27, 2011 - v3
Checksum:iF84163E7CAE415B0
GO
Isoform 5-HT4(A) (identifier: P97288-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     360-388: DTVECGGQWESRCHLTATSPLVAAQPSDT → YTVLHSGHHQELEKLPIHNDPESLESCF

Show »
Length:387
Mass (Da):44,162
Checksum:i2EE398916C432032
GO
Isoform 5-HT4(E) (identifier: P97288-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     359-388: RDTVECGGQWESRCHLTATSPLVAAQPSDT → SFPLLFRNRPVPV

Show »
Length:371
Mass (Da):42,287
Checksum:iD1AD0ACD3D1C8129
GO
Isoform 5-HT4(F) (identifier: P97288-4) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     360-388: DTVECGGQWESRCHLTATSPLVAAQPSDT → PVPV

Show »
Length:363
Mass (Da):41,312
Checksum:i2C8C6F05B22CF91C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti361 – 3611T → A in CAA70773. (PubMed:8946946)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei359 – 38830RDTVE…QPSDT → SFPLLFRNRPVPV in isoform 5-HT4(E). CuratedVSP_001852Add
BLAST
Alternative sequencei360 – 38829DTVEC…QPSDT → YTVLHSGHHQELEKLPIHND PESLESCF in isoform 5-HT4(A). CuratedVSP_001851Add
BLAST
Alternative sequencei360 – 38829DTVEC…QPSDT → PVPV in isoform 5-HT4(F). CuratedVSP_001853Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09585 mRNA. Translation: CAA70773.1.
Y09587 mRNA. Translation: CAA70775.1.
Y09588 mRNA. Translation: CAA70776.1.
AJ011369 mRNA. Translation: CAA09598.1.
AK141587 mRNA. Translation: BAE24750.1.
AK141625 mRNA. Translation: BAE24773.1.
CH466528 Genomic DNA. Translation: EDL09743.1.
CCDSiCCDS29291.1. [P97288-1]
RefSeqiNP_032339.2. NM_008313.4. [P97288-1]
XP_006525738.1. XM_006525675.1. [P97288-1]
XP_006525739.1. XM_006525676.1. [P97288-1]
XP_006525740.1. XM_006525677.1. [P97288-1]
XP_006525741.1. XM_006525678.1. [P97288-1]
XP_006525742.1. XM_006525679.1. [P97288-1]
XP_006525743.1. XM_006525680.1. [P97288-2]
XP_006525746.1. XM_006525683.1. [P97288-3]
UniGeneiMm.20440.

Genome annotation databases

EnsembliENSMUST00000027560; ENSMUSP00000027560; ENSMUSG00000026322. [P97288-1]
GeneIDi15562.
KEGGimmu:15562.
UCSCiuc008fda.1. mouse. [P97288-1]
uc008fdb.1. mouse. [P97288-2]
uc008fdc.1. mouse. [P97288-4]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y09585 mRNA. Translation: CAA70773.1 .
Y09587 mRNA. Translation: CAA70775.1 .
Y09588 mRNA. Translation: CAA70776.1 .
AJ011369 mRNA. Translation: CAA09598.1 .
AK141587 mRNA. Translation: BAE24750.1 .
AK141625 mRNA. Translation: BAE24773.1 .
CH466528 Genomic DNA. Translation: EDL09743.1 .
CCDSi CCDS29291.1. [P97288-1 ]
RefSeqi NP_032339.2. NM_008313.4. [P97288-1 ]
XP_006525738.1. XM_006525675.1. [P97288-1 ]
XP_006525739.1. XM_006525676.1. [P97288-1 ]
XP_006525740.1. XM_006525677.1. [P97288-1 ]
XP_006525741.1. XM_006525678.1. [P97288-1 ]
XP_006525742.1. XM_006525679.1. [P97288-1 ]
XP_006525743.1. XM_006525680.1. [P97288-2 ]
XP_006525746.1. XM_006525683.1. [P97288-3 ]
UniGenei Mm.20440.

3D structure databases

ProteinModelPortali P97288.
SMRi P97288. Positions 25-328.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P97288. 5 interactions.

Chemistry

BindingDBi P97288.
ChEMBLi CHEMBL2183.
GuidetoPHARMACOLOGYi 9.

Protein family/group databases

GPCRDBi Search...

PTM databases

PhosphoSitei P97288.

Proteomic databases

PRIDEi P97288.

Protocols and materials databases

DNASUi 15562.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000027560 ; ENSMUSP00000027560 ; ENSMUSG00000026322 . [P97288-1 ]
GeneIDi 15562.
KEGGi mmu:15562.
UCSCi uc008fda.1. mouse. [P97288-1 ]
uc008fdb.1. mouse. [P97288-2 ]
uc008fdc.1. mouse. [P97288-4 ]

Organism-specific databases

CTDi 3360.
MGIi MGI:109246. Htr4.

Phylogenomic databases

eggNOGi NOG262978.
GeneTreei ENSGT00760000118774.
HOGENOMi HOG000239242.
HOVERGENi HBG106962.
InParanoidi P97288.
KOi K04160.
OMAi YRIYITA.
OrthoDBi EOG7CG707.
TreeFami TF316350.

Miscellaneous databases

NextBioi 288518.
PROi P97288.
SOURCEi Search...

Gene expression databases

Bgeei P97288.
CleanExi MM_HTR4.
Genevestigatori P97288.

Family and domain databases

Gene3Di 1.20.1070.10. 1 hit.
InterProi IPR001520. 5HT4_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view ]
PANTHERi PTHR24249:SF33. PTHR24249:SF33. 1 hit.
Pfami PF00001. 7tm_1. 1 hit.
[Graphical view ]
PRINTSi PR01059. 5HT4RECEPTR.
PR00237. GPCRRHODOPSN.
PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor."
    Claeysen S., Sebben M., Journot L., Bockaert J., Dumuis A.
    FEBS Lett. 398:19-25(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Swiss.
    Tissue: Brain.
  2. Dumuis A.
    Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  3. "5-HT4 receptors: cloning and expression of new splice variants."
    Claeysen S., Faye P., Sebben M., Taviaux S., Bockaert J., Dumuis A.
    Ann. N. Y. Acad. Sci. 861:49-56(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5-HT4(A) AND 5-HT4(E)).
    Strain: Swiss.
    Tissue: Brain.
  4. "Novel brain-specific 5-HT4 receptor splice variants show marked constitutive activity: role of the C-terminal intracellular domain."
    Claeysen S., Sebben M., Becamel C., Bockaert J., Dumuis A.
    Mol. Pharmacol. 55:910-920(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5-HT4(F)).
    Tissue: Brain.
  5. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "New sorting nexin (SNX27) and NHERF specifically interact with the 5-HT4a receptor splice variant: roles in receptor targeting."
    Joubert L., Hanson B., Barthet G., Sebben M., Claeysen S., Hong W., Marin P., Dumuis A., Bockaert J.
    J. Cell Sci. 117:5367-5379(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH INADL; MAGI2; MPP3; NOS1; SEC23A; SLC9A3R1 AND SNX27.

Entry informationi

Entry namei5HT4R_MOUSE
AccessioniPrimary (citable) accession number: P97288
Secondary accession number(s): O89003
, O89004, Q3URB5, Q9R2A4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3