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Reviewed, UniProtKB/Swiss-Prot P97288 (5HT4R_MOUSE)

Last modified June 16, 2009. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    5-hydroxytryptamine receptor 4
      Short name=5-HT-4
      Short name=5-HT4
Alternative name(s):
    Serotonin receptor 4
Gene names
Name: Htr4
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase By similarity.

Subunit structure

Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with INADL, NOS1 and SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR. Ref.5

Subcellular location

Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

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Ontologies

Keywords
   Cellular componentCell membrane
Endosome
Membrane
   Coding sequence diversityAlternative splicing
   DomainTransmembrane
   Molecular functionG-protein coupled receptor
Receptor
Transducer
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
Gene Ontology (GO)
   Biological processG-protein coupled receptor protein signaling pathway

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentendosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionG-protein coupled receptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97288-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 5-HT4(A) (identifier: P97288-2)

The sequence of this isoform differs from the canonical sequence as follows:
     360-388: DAVECGGQWESRCHLTATSPLVAAQPSDT → YTVLHSGHHQELEKLPIHNDPESLESCF
Isoform 5-HT4(E) (identifier: P97288-3)

The sequence of this isoform differs from the canonical sequence as follows:
     359-388: RDAVECGGQWESRCHLTATSPLVAAQPSDT → SFPLLFRNRPVPV
Isoform 5-HT4(F) (identifier: P97288-4)

The sequence of this isoform differs from the canonical sequence as follows:
     360-388: DAVECGGQWESRCHLTATSPLVAAQPSDT → PVPV

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3883885-hydroxytryptamine receptor 4
PRO_0000068966

Regions

Topological domain1 – 1919Extracellular By similarity
Transmembrane20 – 40211 By similarity
Topological domain41 – 5818Cytoplasmic By similarity
Transmembrane59 – 79212 By similarity
Topological domain80 – 9314Extracellular By similarity
Transmembrane94 – 116233 By similarity
Topological domain117 – 13721Cytoplasmic By similarity
Transmembrane138 – 158214 By similarity
Topological domain159 – 19234Extracellular By similarity
Transmembrane193 – 213215 By similarity
Topological domain214 – 26047Cytoplasmic By similarity
Transmembrane261 – 281216 By similarity
Topological domain282 – 29413Extracellular By similarity
Transmembrane295 – 315217 By similarity
Topological domain316 – 38873Cytoplasmic By similarity

Amino acid modifications

Lipidation3291S-palmitoyl cysteine By similarity
Glycosylation71N-linked (GlcNAc...) Potential
Disulfide bond93 ↔ 184 By similarity

Natural variations

Alternative sequence359 – 38830RDAVE…QPSDT → SFPLLFRNRPVPV in isoform 5-HT4(E).
VSP_001852
Alternative sequence360 – 38829DAVEC…QPSDT → YTVLHSGHHQELEKLPIHND PESLESCF in isoform 5-HT4(A).
VSP_001851
Alternative sequence360 – 38829DAVEC…QPSDT → PVPV in isoform 5-HT4(F).
VSP_001853

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 15, 1999. Version 2.
Checksum: F84163F39BA115B0

FASTA38843,932
        10         20         30         40         50         60 
MDKLDANVSS NEGFRSVEKV VLLTFLAVVI LMAILGNLLV MVAVCRDRQL RKIKTNYFIV 

        70         80         90        100        110        120 
SLAFADLLVS VLVMPFGAIE LVQDIWAYGE MFCLVRTSLD VLLTTASIFH LCCISLDRYY 

       130        140        150        160        170        180 
AICCQPLVYR NKMTPLRIAL MLGGCWVLPM FISFLPIMQG WNNIGIVDVI EKRKFSHNSN 

       190        200        210        220        230        240 
STWCVFMVNK PYAITCSVVA FYIPFLLMVL AYYRIYVTAK EHAQQIQMLQ RAGATSESRP 

       250        260        270        280        290        300 
QPADQHSTHR MRTETKAAKT LCVIMGCFCF CWAPFFVTNI VDPFIDYTVP EQVWTAFLWL 

       310        320        330        340        350        360 
GYINSGLNPF LYAFLNKSFR RAFLIILCCD DERYKRPPIL GQTVPCSTTT INGSTHVLRD 

       370        380 
AVECGGQWES RCHLTATSPL VAAQPSDT

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Isoform 5-HT4(A).

Checksum: 2EE398916C432032
Show »

FASTA38744,162
Isoform 5-HT4(E).

Checksum: D1AD0ACD3D1C8129
Show »

FASTA37142,287
Isoform 5-HT4(F).

Checksum: 2C8C6F05B22CF91C
Show »

FASTA36341,312

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References

[1]"Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor."
Claeysen S., Sebben M., Journot L., Bockaert J., Dumuis A.
FEBS Lett. 398:19-25(1996) [PubMed: 8946946] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss.
Tissue: Brain.
[2]Dumuis A.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]"5-HT4 receptors: cloning and expression of new splice variants."
Claeysen S., Faye P., Sebben M., Taviaux S., Bockaert J., Dumuis A.
Ann. N. Y. Acad. Sci. 861:49-56(1998) [PubMed: 9928238] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5-HT4(A) AND 5-HT4(E)).
Strain: Swiss.
Tissue: Brain.
[4]"Novel brain-specific 5-HT4 receptor splice variants show marked constitutive activity: role of the C-terminal intracellular domain."
Claeysen S., Sebben M., Becamel C., Bockaert J., Dumuis A.
Mol. Pharmacol. 55:910-920(1999) [PubMed: 10220570] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5-HT4(F)).
Tissue: Brain.
[5]"New sorting nexin (SNX27) and NHERF specifically interact with the 5-HT4a receptor splice variant: roles in receptor targeting."
Joubert L., Hanson B., Barthet G., Sebben M., Claeysen S., Hong W., Marin P., Dumuis A., Bockaert J.
J. Cell Sci. 117:5367-5379(2004) [PubMed: 15466885] [Abstract]
Cited for: INTERACTION WITH INADL; MAGI2; MPP3; NOS1; SEC23A; SLC9A3R1 AND SNX27.
+Additional computationally mapped references.

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Cross-references

Sequence databases

Y09585 mRNA. Translation: CAA70773.1.
Y09587 mRNA. Translation: CAA70775.1.
Y09588 mRNA. Translation: CAA70776.1.
AJ011369 mRNA. Translation: CAA09598.1.
IPIIPI00122961.
IPI00223494.
IPI00223495.
IPI00223496.
UniGeneMm.20440

3D structure databases

HSSPHSSP built from PDB template 1HLL based on UniProtKB P08913.
ModBaseSearch...

Protein family/group databases

GPCRDBSearch...

Genome annotation databases

EnsemblENSMUSG00000026322. Mus musculus. [Contig view]

Organism-specific databases

MGIMGI:109246. Htr4.

Phylogenomic databases

HOGENOMP97288.
HOVERGENP97288.

Gene expression databases

ArrayExpressP97288.
BgeeP97288.
CleanExMM_HTR4.
GermOnlineENSMUSG00000026322. Mus musculus.

Family and domain databases

InterProIPR001520. 5HT4_rcpt.
IPR000276. 7TM_GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_supfam.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01059. 5HT4RECEPTR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

SOURCESearch...

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Entry information

Entry name5HT4R_MOUSE
AccessionPrimary (citable) accession number: P97288
Secondary accession number(s): O89003, O89004, Q9R2A4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 15, 1999
Last modified: June 16, 2009
This is version 78 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents