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P97288 (5HT4R_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
5-hydroxytryptamine receptor 4

Short name=5-HT-4
Short name=5-HT4
Alternative name(s):
Serotonin receptor 4
Gene names
Name:Htr4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length388 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase By similarity.

Subunit structure

Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with INADL, NOS1 and SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR. Interacts (via C-terminus 330-346 AA) with GRK5; this interaction is promoted by 5-HT (serotonin) By similarity. Ref.7

Subcellular location

Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

GRK5P349478EBI-7149283,EBI-7149314From a different organism.
SRCP129313EBI-7149283,EBI-621482From a different organism.

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P97288-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 5-HT4(A) (identifier: P97288-2)

The sequence of this isoform differs from the canonical sequence as follows:
     360-388: DTVECGGQWESRCHLTATSPLVAAQPSDT → YTVLHSGHHQELEKLPIHNDPESLESCF
Isoform 5-HT4(E) (identifier: P97288-3)

The sequence of this isoform differs from the canonical sequence as follows:
     359-388: RDTVECGGQWESRCHLTATSPLVAAQPSDT → SFPLLFRNRPVPV
Isoform 5-HT4(F) (identifier: P97288-4)

The sequence of this isoform differs from the canonical sequence as follows:
     360-388: DTVECGGQWESRCHLTATSPLVAAQPSDT → PVPV

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 3883885-hydroxytryptamine receptor 4
PRO_0000068966

Regions

Topological domain1 – 1919Extracellular By similarity
Transmembrane20 – 4021Helical; Name=1; By similarity
Topological domain41 – 5818Cytoplasmic By similarity
Transmembrane59 – 7921Helical; Name=2; By similarity
Topological domain80 – 9314Extracellular By similarity
Transmembrane94 – 11623Helical; Name=3; By similarity
Topological domain117 – 13721Cytoplasmic By similarity
Transmembrane138 – 15821Helical; Name=4; By similarity
Topological domain159 – 19234Extracellular By similarity
Transmembrane193 – 21321Helical; Name=5; By similarity
Topological domain214 – 26047Cytoplasmic By similarity
Transmembrane261 – 28121Helical; Name=6; By similarity
Topological domain282 – 29413Extracellular By similarity
Transmembrane295 – 31521Helical; Name=7; By similarity
Topological domain316 – 38873Cytoplasmic By similarity

Amino acid modifications

Lipidation3291S-palmitoyl cysteine By similarity
Glycosylation71N-linked (GlcNAc...) Potential
Disulfide bond93 ↔ 184 By similarity

Natural variations

Alternative sequence359 – 38830RDTVE…QPSDT → SFPLLFRNRPVPV in isoform 5-HT4(E).
VSP_001852
Alternative sequence360 – 38829DTVEC…QPSDT → YTVLHSGHHQELEKLPIHND PESLESCF in isoform 5-HT4(A).
VSP_001851
Alternative sequence360 – 38829DTVEC…QPSDT → PVPV in isoform 5-HT4(F).
VSP_001853

Experimental info

Sequence conflict3611T → A in CAA70773. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: F84163E7CAE415B0

FASTA38843,962
        10         20         30         40         50         60 
MDKLDANVSS NEGFRSVEKV VLLTFLAVVI LMAILGNLLV MVAVCRDRQL RKIKTNYFIV 

        70         80         90        100        110        120 
SLAFADLLVS VLVMPFGAIE LVQDIWAYGE MFCLVRTSLD VLLTTASIFH LCCISLDRYY 

       130        140        150        160        170        180 
AICCQPLVYR NKMTPLRIAL MLGGCWVLPM FISFLPIMQG WNNIGIVDVI EKRKFSHNSN 

       190        200        210        220        230        240 
STWCVFMVNK PYAITCSVVA FYIPFLLMVL AYYRIYVTAK EHAQQIQMLQ RAGATSESRP 

       250        260        270        280        290        300 
QPADQHSTHR MRTETKAAKT LCVIMGCFCF CWAPFFVTNI VDPFIDYTVP EQVWTAFLWL 

       310        320        330        340        350        360 
GYINSGLNPF LYAFLNKSFR RAFLIILCCD DERYKRPPIL GQTVPCSTTT INGSTHVLRD 

       370        380 
TVECGGQWES RCHLTATSPL VAAQPSDT 

« Hide

Isoform 5-HT4(A) [UniParc].

Checksum: 2EE398916C432032
Show »

FASTA38744,162
Isoform 5-HT4(E) [UniParc].

Checksum: D1AD0ACD3D1C8129
Show »

FASTA37142,287
Isoform 5-HT4(F) [UniParc].

Checksum: 2C8C6F05B22CF91C
Show »

FASTA36341,312

References

« Hide 'large scale' references
[1]"Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor."
Claeysen S., Sebben M., Journot L., Bockaert J., Dumuis A.
FEBS Lett. 398:19-25(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Swiss.
Tissue: Brain.
[2]Dumuis A.
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[3]"5-HT4 receptors: cloning and expression of new splice variants."
Claeysen S., Faye P., Sebben M., Taviaux S., Bockaert J., Dumuis A.
Ann. N. Y. Acad. Sci. 861:49-56(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5-HT4(A) AND 5-HT4(E)).
Strain: Swiss.
Tissue: Brain.
[4]"Novel brain-specific 5-HT4 receptor splice variants show marked constitutive activity: role of the C-terminal intracellular domain."
Claeysen S., Sebben M., Becamel C., Bockaert J., Dumuis A.
Mol. Pharmacol. 55:910-920(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5-HT4(F)).
Tissue: Brain.
[5]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"New sorting nexin (SNX27) and NHERF specifically interact with the 5-HT4a receptor splice variant: roles in receptor targeting."
Joubert L., Hanson B., Barthet G., Sebben M., Claeysen S., Hong W., Marin P., Dumuis A., Bockaert J.
J. Cell Sci. 117:5367-5379(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH INADL; MAGI2; MPP3; NOS1; SEC23A; SLC9A3R1 AND SNX27.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09585 mRNA. Translation: CAA70773.1.
Y09587 mRNA. Translation: CAA70775.1.
Y09588 mRNA. Translation: CAA70776.1.
AJ011369 mRNA. Translation: CAA09598.1.
AK141587 mRNA. Translation: BAE24750.1.
AK141625 mRNA. Translation: BAE24773.1.
CH466528 Genomic DNA. Translation: EDL09743.1.
CCDSCCDS29291.1. [P97288-1]
RefSeqNP_032339.2. NM_008313.4. [P97288-1]
XP_006525738.1. XM_006525675.1. [P97288-1]
XP_006525739.1. XM_006525676.1. [P97288-1]
XP_006525740.1. XM_006525677.1. [P97288-1]
XP_006525741.1. XM_006525678.1. [P97288-1]
XP_006525742.1. XM_006525679.1. [P97288-1]
XP_006525743.1. XM_006525680.1. [P97288-2]
XP_006525746.1. XM_006525683.1. [P97288-3]
UniGeneMm.20440.

3D structure databases

ProteinModelPortalP97288.
SMRP97288. Positions 25-328.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP97288. 4 interactions.

Chemistry

BindingDBP97288.
ChEMBLCHEMBL2183.
GuidetoPHARMACOLOGY9.

Protein family/group databases

GPCRDBSearch...

PTM databases

PhosphoSiteP97288.

Proteomic databases

PRIDEP97288.

Protocols and materials databases

DNASU15562.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000027560; ENSMUSP00000027560; ENSMUSG00000026322. [P97288-1]
GeneID15562.
KEGGmmu:15562.
UCSCuc008fda.1. mouse. [P97288-1]
uc008fdb.1. mouse. [P97288-2]
uc008fdc.1. mouse. [P97288-4]

Organism-specific databases

CTD3360.
MGIMGI:109246. Htr4.

Phylogenomic databases

eggNOGNOG262978.
GeneTreeENSGT00720000108411.
HOGENOMHOG000239242.
HOVERGENHBG106962.
InParanoidQ3URB5.
KOK04160.
OMAYRIYITA.
OrthoDBEOG7CG707.
TreeFamTF316350.

Gene expression databases

BgeeP97288.
CleanExMM_HTR4.
GenevestigatorP97288.

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR001520. 5HT4_rcpt.
IPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PANTHERPTHR24249:SF33. PTHR24249:SF33. 1 hit.
PfamPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSPR01059. 5HT4RECEPTR.
PR00237. GPCRRHODOPSN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio288518.
PROP97288.
SOURCESearch...

Entry information

Entry name5HT4R_MOUSE
AccessionPrimary (citable) accession number: P97288
Secondary accession number(s): O89003 expand/collapse secondary AC list , O89004, Q3URB5, Q9R2A4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries