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P97288

- 5HT4R_MOUSE

UniProt

P97288 - 5HT4R_MOUSE

Protein

5-hydroxytryptamine receptor 4

Gene

Htr4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase By similarity.By similarity

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. serotonin receptor activity Source: Ensembl

    GO - Biological processi

    1. positive regulation of cell proliferation Source: Ensembl
    2. regulation of appetite Source: InterPro
    3. synaptic transmission Source: InterPro

    Keywords - Molecular functioni

    G-protein coupled receptor, Receptor, Transducer

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    5-hydroxytryptamine receptor 4
    Short name:
    5-HT-4
    Short name:
    5-HT4
    Alternative name(s):
    Serotonin receptor 4
    Gene namesi
    Name:Htr4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 18

    Organism-specific databases

    MGIiMGI:109246. Htr4.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Endosome
    Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.

    GO - Cellular componenti

    1. endosome Source: UniProtKB-SubCell
    2. integral component of plasma membrane Source: InterPro

    Keywords - Cellular componenti

    Cell membrane, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 3883885-hydroxytryptamine receptor 4PRO_0000068966Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi7 – 71N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi93 ↔ 184PROSITE-ProRule annotation
    Lipidationi329 – 3291S-palmitoyl cysteineBy similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

    Proteomic databases

    PRIDEiP97288.

    PTM databases

    PhosphoSiteiP97288.

    Expressioni

    Gene expression databases

    BgeeiP97288.
    CleanExiMM_HTR4.
    GenevestigatoriP97288.

    Interactioni

    Subunit structurei

    Isoform 5-HT4(A) interacts with MAGI2, MPP3, SLC9A3R1 and SNX27 isoforms 1 and 2. Isoform 5-HT4(E) interacts with INADL, NOS1 and SEC23A. Isoform 5-HT4(A) forms a complex including SLC9A3R1 and EZR. Interacts (via C-terminus 330-346 AA) with GRK5; this interaction is promoted by 5-HT (serotonin) By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    GRK5P349478EBI-7149283,EBI-7149314From a different organism.
    SRCP129312EBI-7149283,EBI-621482From a different organism.

    Protein-protein interaction databases

    IntActiP97288. 5 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP97288.
    SMRiP97288. Positions 25-328.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1919ExtracellularBy similarityAdd
    BLAST
    Topological domaini41 – 5818CytoplasmicBy similarityAdd
    BLAST
    Topological domaini80 – 9314ExtracellularBy similarityAdd
    BLAST
    Topological domaini117 – 13721CytoplasmicBy similarityAdd
    BLAST
    Topological domaini159 – 19234ExtracellularBy similarityAdd
    BLAST
    Topological domaini214 – 26047CytoplasmicBy similarityAdd
    BLAST
    Topological domaini282 – 29413ExtracellularBy similarityAdd
    BLAST
    Topological domaini316 – 38873CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei20 – 4021Helical; Name=1By similarityAdd
    BLAST
    Transmembranei59 – 7921Helical; Name=2By similarityAdd
    BLAST
    Transmembranei94 – 11623Helical; Name=3By similarityAdd
    BLAST
    Transmembranei138 – 15821Helical; Name=4By similarityAdd
    BLAST
    Transmembranei193 – 21321Helical; Name=5By similarityAdd
    BLAST
    Transmembranei261 – 28121Helical; Name=6By similarityAdd
    BLAST
    Transmembranei295 – 31521Helical; Name=7By similarityAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG262978.
    GeneTreeiENSGT00720000108411.
    HOGENOMiHOG000239242.
    HOVERGENiHBG106962.
    InParanoidiQ3URB5.
    KOiK04160.
    OMAiYRIYITA.
    OrthoDBiEOG7CG707.
    TreeFamiTF316350.

    Family and domain databases

    Gene3Di1.20.1070.10. 1 hit.
    InterProiIPR001520. 5HT4_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view]
    PANTHERiPTHR24249:SF33. PTHR24249:SF33. 1 hit.
    PfamiPF00001. 7tm_1. 1 hit.
    [Graphical view]
    PRINTSiPR01059. 5HT4RECEPTR.
    PR00237. GPCRRHODOPSN.
    PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view]

    Sequences (4)i

    Sequence statusi: Complete.

    This entry describes 4 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P97288-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MDKLDANVSS NEGFRSVEKV VLLTFLAVVI LMAILGNLLV MVAVCRDRQL    50
    RKIKTNYFIV SLAFADLLVS VLVMPFGAIE LVQDIWAYGE MFCLVRTSLD 100
    VLLTTASIFH LCCISLDRYY AICCQPLVYR NKMTPLRIAL MLGGCWVLPM 150
    FISFLPIMQG WNNIGIVDVI EKRKFSHNSN STWCVFMVNK PYAITCSVVA 200
    FYIPFLLMVL AYYRIYVTAK EHAQQIQMLQ RAGATSESRP QPADQHSTHR 250
    MRTETKAAKT LCVIMGCFCF CWAPFFVTNI VDPFIDYTVP EQVWTAFLWL 300
    GYINSGLNPF LYAFLNKSFR RAFLIILCCD DERYKRPPIL GQTVPCSTTT 350
    INGSTHVLRD TVECGGQWES RCHLTATSPL VAAQPSDT 388
    Length:388
    Mass (Da):43,962
    Last modified:July 27, 2011 - v3
    Checksum:iF84163E7CAE415B0
    GO
    Isoform 5-HT4(A) (identifier: P97288-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         360-388: DTVECGGQWESRCHLTATSPLVAAQPSDT → YTVLHSGHHQELEKLPIHNDPESLESCF

    Show »
    Length:387
    Mass (Da):44,162
    Checksum:i2EE398916C432032
    GO
    Isoform 5-HT4(E) (identifier: P97288-3) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         359-388: RDTVECGGQWESRCHLTATSPLVAAQPSDT → SFPLLFRNRPVPV

    Show »
    Length:371
    Mass (Da):42,287
    Checksum:iD1AD0ACD3D1C8129
    GO
    Isoform 5-HT4(F) (identifier: P97288-4) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         360-388: DTVECGGQWESRCHLTATSPLVAAQPSDT → PVPV

    Show »
    Length:363
    Mass (Da):41,312
    Checksum:i2C8C6F05B22CF91C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti361 – 3611T → A in CAA70773. (PubMed:8946946)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei359 – 38830RDTVE…QPSDT → SFPLLFRNRPVPV in isoform 5-HT4(E). CuratedVSP_001852Add
    BLAST
    Alternative sequencei360 – 38829DTVEC…QPSDT → YTVLHSGHHQELEKLPIHND PESLESCF in isoform 5-HT4(A). CuratedVSP_001851Add
    BLAST
    Alternative sequencei360 – 38829DTVEC…QPSDT → PVPV in isoform 5-HT4(F). CuratedVSP_001853Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09585 mRNA. Translation: CAA70773.1.
    Y09587 mRNA. Translation: CAA70775.1.
    Y09588 mRNA. Translation: CAA70776.1.
    AJ011369 mRNA. Translation: CAA09598.1.
    AK141587 mRNA. Translation: BAE24750.1.
    AK141625 mRNA. Translation: BAE24773.1.
    CH466528 Genomic DNA. Translation: EDL09743.1.
    CCDSiCCDS29291.1. [P97288-1]
    RefSeqiNP_032339.2. NM_008313.4. [P97288-1]
    XP_006525738.1. XM_006525675.1. [P97288-1]
    XP_006525739.1. XM_006525676.1. [P97288-1]
    XP_006525740.1. XM_006525677.1. [P97288-1]
    XP_006525741.1. XM_006525678.1. [P97288-1]
    XP_006525742.1. XM_006525679.1. [P97288-1]
    XP_006525743.1. XM_006525680.1. [P97288-2]
    XP_006525746.1. XM_006525683.1. [P97288-3]
    UniGeneiMm.20440.

    Genome annotation databases

    EnsembliENSMUST00000027560; ENSMUSP00000027560; ENSMUSG00000026322. [P97288-1]
    GeneIDi15562.
    KEGGimmu:15562.
    UCSCiuc008fda.1. mouse. [P97288-1]
    uc008fdb.1. mouse. [P97288-2]
    uc008fdc.1. mouse. [P97288-4]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y09585 mRNA. Translation: CAA70773.1 .
    Y09587 mRNA. Translation: CAA70775.1 .
    Y09588 mRNA. Translation: CAA70776.1 .
    AJ011369 mRNA. Translation: CAA09598.1 .
    AK141587 mRNA. Translation: BAE24750.1 .
    AK141625 mRNA. Translation: BAE24773.1 .
    CH466528 Genomic DNA. Translation: EDL09743.1 .
    CCDSi CCDS29291.1. [P97288-1 ]
    RefSeqi NP_032339.2. NM_008313.4. [P97288-1 ]
    XP_006525738.1. XM_006525675.1. [P97288-1 ]
    XP_006525739.1. XM_006525676.1. [P97288-1 ]
    XP_006525740.1. XM_006525677.1. [P97288-1 ]
    XP_006525741.1. XM_006525678.1. [P97288-1 ]
    XP_006525742.1. XM_006525679.1. [P97288-1 ]
    XP_006525743.1. XM_006525680.1. [P97288-2 ]
    XP_006525746.1. XM_006525683.1. [P97288-3 ]
    UniGenei Mm.20440.

    3D structure databases

    ProteinModelPortali P97288.
    SMRi P97288. Positions 25-328.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P97288. 5 interactions.

    Chemistry

    BindingDBi P97288.
    ChEMBLi CHEMBL2183.
    GuidetoPHARMACOLOGYi 9.

    Protein family/group databases

    GPCRDBi Search...

    PTM databases

    PhosphoSitei P97288.

    Proteomic databases

    PRIDEi P97288.

    Protocols and materials databases

    DNASUi 15562.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027560 ; ENSMUSP00000027560 ; ENSMUSG00000026322 . [P97288-1 ]
    GeneIDi 15562.
    KEGGi mmu:15562.
    UCSCi uc008fda.1. mouse. [P97288-1 ]
    uc008fdb.1. mouse. [P97288-2 ]
    uc008fdc.1. mouse. [P97288-4 ]

    Organism-specific databases

    CTDi 3360.
    MGIi MGI:109246. Htr4.

    Phylogenomic databases

    eggNOGi NOG262978.
    GeneTreei ENSGT00720000108411.
    HOGENOMi HOG000239242.
    HOVERGENi HBG106962.
    InParanoidi Q3URB5.
    KOi K04160.
    OMAi YRIYITA.
    OrthoDBi EOG7CG707.
    TreeFami TF316350.

    Miscellaneous databases

    NextBioi 288518.
    PROi P97288.
    SOURCEi Search...

    Gene expression databases

    Bgeei P97288.
    CleanExi MM_HTR4.
    Genevestigatori P97288.

    Family and domain databases

    Gene3Di 1.20.1070.10. 1 hit.
    InterProi IPR001520. 5HT4_rcpt.
    IPR000276. GPCR_Rhodpsn.
    IPR017452. GPCR_Rhodpsn_7TM.
    [Graphical view ]
    PANTHERi PTHR24249:SF33. PTHR24249:SF33. 1 hit.
    Pfami PF00001. 7tm_1. 1 hit.
    [Graphical view ]
    PRINTSi PR01059. 5HT4RECEPTR.
    PR00237. GPCRRHODOPSN.
    PROSITEi PS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
    PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning, expression and pharmacology of the mouse 5-HT(4L) receptor."
      Claeysen S., Sebben M., Journot L., Bockaert J., Dumuis A.
      FEBS Lett. 398:19-25(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Swiss.
      Tissue: Brain.
    2. Dumuis A.
      Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    3. "5-HT4 receptors: cloning and expression of new splice variants."
      Claeysen S., Faye P., Sebben M., Taviaux S., Bockaert J., Dumuis A.
      Ann. N. Y. Acad. Sci. 861:49-56(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 5-HT4(A) AND 5-HT4(E)).
      Strain: Swiss.
      Tissue: Brain.
    4. "Novel brain-specific 5-HT4 receptor splice variants show marked constitutive activity: role of the C-terminal intracellular domain."
      Claeysen S., Sebben M., Becamel C., Bockaert J., Dumuis A.
      Mol. Pharmacol. 55:910-920(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 5-HT4(F)).
      Tissue: Brain.
    5. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    7. "New sorting nexin (SNX27) and NHERF specifically interact with the 5-HT4a receptor splice variant: roles in receptor targeting."
      Joubert L., Hanson B., Barthet G., Sebben M., Claeysen S., Hong W., Marin P., Dumuis A., Bockaert J.
      J. Cell Sci. 117:5367-5379(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH INADL; MAGI2; MPP3; NOS1; SEC23A; SLC9A3R1 AND SNX27.

    Entry informationi

    Entry namei5HT4R_MOUSE
    AccessioniPrimary (citable) accession number: P97288
    Secondary accession number(s): O89003
    , O89004, Q3URB5, Q9R2A4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 126 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. 7-transmembrane G-linked receptors
      List of 7-transmembrane G-linked receptor entries
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3