Reviewed,
UniProtKB/Swiss-Prot P97287 (MCL1_MOUSE)
Last modified
November 25, 2008.
Version 74.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Induced myeloid leukemia cell differentiation protein Mcl-1 homolog Alternative name(s): Bcl-2-related protein EAT/mcl1 | ||
| Gene names |
| ||
| Organism | Mus musculus (Mouse) | ||
| Taxonomic identifier | 10090 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus |
Protein attributes
| Sequence length | 331 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. |
| Subunit structure | Interacts with BAD, BOK, BIK, BAX, BAK1, and TPT1 By similarity. Interacts with BBC3, BCL2L11, BFM and PMAIP1. |
| Subcellular location | Membrane; Single-pass membrane proteinBy similarity. CytoplasmBy similarity. MitochondrionBy similarity. Nucleus › nucleoplasmBy similarity. Note= Cytoplasmic, associated with mitochondria. |
| Induction | Up-regulated by IL3 and CSF2. Up-regulated in murine embryonal carcinoma cells in response to retinoic acid treatment. Levels reach a maximum after 4 hours, are decreased after 8 hours and are back to maximum after 12 hours. Levels are decreased after 24 hours and back to basal levels after 48 hours. Expression remains constant in retinoic acid-treated embryonic stem cells. |
| Post-translational modification | Cleaved by CASP3 during apoptosis, yielding a pro-apoptotic C-terminal fragment By similarity. Rapidly degraded in the absence of phosphorylation in the PEST region By similarity. Phosphorylated on several Ser and Thr residues By similarity. |
| Sequence similarities | Belongs to the Bcl-2 family. |
Ontologies
Keywords | |
|---|---|
| Biological process | Apoptosis Differentiation |
| Cellular component | Cytoplasm Membrane Mitochondrion Nucleus |
| Domain | Transmembrane |
| Molecular function | Developmental protein |
| PTM | Phosphoprotein Ubl conjugation |
| Technical term | 3D-structure |
Gene Ontology (GO) | |
| Biological process | cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal developmentInferred from electronic annotation. Source: UniProtKB-KW regulation of apoptosisInferred from electronic annotation. Source: InterPro |
| Cellular component | integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrionInferred from electronic annotation. Source: UniProtKB-KW nucleusInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| BAK1 | Q16611 | 3 | EBI-707292,EBI-519866 | From a different organism. |
| BBC3 | Q9BXH1 | 2 | EBI-707292,EBI-519884 | From a different organism. |
| BCL2L11 | O43521 | 3 | EBI-707292,EBI-526406 | From a different organism. |
| Bcl2l11 | O54918 | 1 | EBI-707292,EBI-526067 | |
| PMAIP1 | Q13794 | 1 | EBI-707292,EBI-707392 | From a different organism. |
| Pmaip1 | Q9JM54 | 1 | EBI-707292,EBI-709183 |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | |||||||||||||||||||||||
Molecule processing | ||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 331 | 331 | Induced myeloid leukemia cell differentiation protein Mcl-1 homolog | PRO_0000143081 | ||||||||||||||||||||||||
Regions | ||||||||||||||||||||||||||||
| Transmembrane | 308 – 330 | 23 | Potential | |||||||||||||||||||||||||
| Region | 85 – 156 | 72 | PEST-like By similarity | |||||||||||||||||||||||||
| Motif | 190 – 204 | 15 | BH3 | |||||||||||||||||||||||||
| Motif | 233 – 253 | 21 | BH1 | |||||||||||||||||||||||||
| Motif | 285 – 300 | 16 | BH2 | |||||||||||||||||||||||||
| Compositional bias | 149 – 152 | 4 | Poly-Glu | |||||||||||||||||||||||||
Sites | ||||||||||||||||||||||||||||
| Site | 108 – 109 | 2 | Cleavage; by caspase-3 By similarity | |||||||||||||||||||||||||
| Site | 138 – 139 | 2 | Cleavage; by caspase-3 By similarity | |||||||||||||||||||||||||
Amino acid modifications | ||||||||||||||||||||||||||||
| Modified residue | 102 | 1 | Phosphoserine By similarity | |||||||||||||||||||||||||
| Modified residue | 144 | 1 | Phosphothreonine; by MAPK By similarity | |||||||||||||||||||||||||
| Cross-link | 117 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity | ||||||||||||||||||||||||||
| Cross-link | 175 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity | ||||||||||||||||||||||||||
| Cross-link | 178 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) By similarity | ||||||||||||||||||||||||||
Secondary structure | ||||||||||||||||||||||||||||
Helix Strand Turn | ||||||||||||||||||||||||||||
| Helix | 154 – 172 | 19 | ||||||||||||||||||||||||||
| Beta strand | 181 – 183 | 3 | ||||||||||||||||||||||||||
| Helix | 185 – 215 | 31 | ||||||||||||||||||||||||||
| Turn | 221 – 223 | 3 | ||||||||||||||||||||||||||
| Helix | 224 – 234 | 11 | ||||||||||||||||||||||||||
| Beta strand | 237 – 239 | 3 | ||||||||||||||||||||||||||
| Helix | 242 – 261 | 20 | ||||||||||||||||||||||||||
| Helix | 265 – 282 | 18 | ||||||||||||||||||||||||||
| Helix | 284 – 289 | 6 | ||||||||||||||||||||||||||
| Turn | 290 – 292 | 3 | ||||||||||||||||||||||||||
| Helix | 293 – 299 | 7 | ||||||||||||||||||||||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Up-regulated expression of murine Mcl1/EAT, a bcl-2 related gene, in the early stage of differentiation of murine embryonal carcinoma cells and embryonic stem cells." Okita H., Umezawa A., Suzuki A., Hata J. Biochim. Biophys. Acta 1398:335-341(1998) [PubMed: 9655929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], INDUCTION. Tissue: Fetus. |
| [2] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo and Embryonic stem cell. |
| [3] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II and FVB/N. Tissue: Mammary tumor and Salivary gland. |
| [4] | "Mcl-1 is an immediate-early gene activated by the granulocyte-macrophage colony-stimulating factor (GM-CSF) signaling pathway and is one component of the GM-CSF viability response." Chao J.-R., Wang J.-M., Lee S.-F., Peng H.-W., Lin Y.-H., Chou C.-H., Li J.-C., Huang H.-M., Chou C.-K., Kuo M.-L., Yen J.J.-Y., Yang-Yen H.-F. Mol. Cell. Biol. 18:4883-4898(1998) [PubMed: 9671497] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-65, INDUCTION. Strain: 129/SvJ. |
| [5] | "Solution structure of prosurvival Mcl-1 and characterization of its binding by proapoptotic BH3-only ligands." Day C.L., Chen L., Richardson S.J., Harrison P.J., Huang D.C.S., Hinds M.G. J. Biol. Chem. 280:4738-4744(2005) [PubMed: 15550399] [Abstract] Cited for: STRUCTURE BY NMR OF 152-308, INTERACTION WITH BCL2L11; BMF AND PMAIP. |
| [6] | "Structure of the BH3 domains from the p53-inducible BH3-only proteins Noxa and Puma in complex with Mcl-1." Day C.L., Smits C., Fan F.C., Lee E.F., Fairlie W.D., Hinds M.G. J. Mol. Biol. 380:958-971(2008) [PubMed: 18589438] [Abstract] Cited for: STRUCTURE BY NMR OF 148-308 IN COMPLEXES WITH WITH BBC3 AND PMAIP1. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| U35623 mRNA. Translation: AAC31790.1. AK010424 mRNA. Translation: BAB26927.1. AK159504 mRNA. Translation: BAE35137.1. AK160594 mRNA. Translation: BAE35901.1. BC003839 mRNA. Translation: AAH03839.1. BC005427 mRNA. Translation: AAH05427.1. BC021638 mRNA. Translation: AAH21638.1. AF063886 Genomic DNA. Translation: AAC27929.1. | |||||||||||||||||||||||||||||||
| RefSeq | NP_032588.1. | ||||||||||||||||||||||||||||||
| UniGene | Mm.1639 | ||||||||||||||||||||||||||||||
3D structure databases | |||||||||||||||||||||||||||||||
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| ModBase | Search... | ||||||||||||||||||||||||||||||
Protein-protein interaction databases | |||||||||||||||||||||||||||||||
| IntAct | P97287. | ||||||||||||||||||||||||||||||
PTM databases | |||||||||||||||||||||||||||||||
| PhosphoSite | P97287. | ||||||||||||||||||||||||||||||
Genome annotation databases | |||||||||||||||||||||||||||||||
| Ensembl | ENSMUSG00000038612. Mus musculus. [Contig view] | ||||||||||||||||||||||||||||||
| GeneID | 17210. | ||||||||||||||||||||||||||||||
| KEGG | mmu:17210. | ||||||||||||||||||||||||||||||
Organism-specific databases | |||||||||||||||||||||||||||||||
| MGI | MGI:101769. Mcl1. | ||||||||||||||||||||||||||||||
Phylogenomic databases | |||||||||||||||||||||||||||||||
| HOGENOM | P97287. | ||||||||||||||||||||||||||||||
| HOVERGEN | P97287. | ||||||||||||||||||||||||||||||
Gene expression databases | |||||||||||||||||||||||||||||||
| ArrayExpress | P97287. | ||||||||||||||||||||||||||||||
| CleanEx | MM_MCL1. | ||||||||||||||||||||||||||||||
| GermOnline | ENSMUSG00000038612. Mus musculus. | ||||||||||||||||||||||||||||||
Family and domain databases | |||||||||||||||||||||||||||||||
| InterPro | IPR013281. Apop_reg_Mc1. IPR002475. BCL2_apoptsis. IPR000712. Bcl2_BH. [Graphical view] | ||||||||||||||||||||||||||||||
| Pfam | PF00452. Bcl-2. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| PRINTS | PR01866. APOPREGMCL1. PR01862. BCL2FAMILY. | ||||||||||||||||||||||||||||||
| SMART | SM00337. BCL. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| PROSITE | PS50062. BCL2_FAMILY. 1 hit. PS01080. BH1. 1 hit. PS01258. BH2. 1 hit. PS01259. BH3. 1 hit. [Graphical view] | ||||||||||||||||||||||||||||||
| ProtoNet | Search... | ||||||||||||||||||||||||||||||
Other Resources | |||||||||||||||||||||||||||||||
| NextBio | 291598. | ||||||||||||||||||||||||||||||
| SOURCE | Search... | ||||||||||||||||||||||||||||||
Entry information
| Entry name | MCL1_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P97287 Secondary accession number(s): Q3TUS0, Q792P0, Q9CRI4 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PDB cross-references Index of Protein Data Bank (PDB) cross-references |
| SIMILARITY comments Index of protein domains and families |

Clusters with


