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Protein

Alkyldihydroxyacetonephosphate synthase, peroxisomal

Gene

AGPS

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the exchange of an acyl for a long-chain alkyl group and the formation of the ether bond in the biosynthesis of ether phospholipids.1 Publication

Catalytic activityi

1-acyl-glycerone 3-phosphate + a long-chain alcohol = an alkyl-glycerone 3-phosphate + a long-chain acid anion.1 Publication

Cofactori

FAD1 Publication

Pathwayi: ether lipid biosynthesis

This protein is involved in the pathway ether lipid biosynthesis, which is part of Glycerolipid metabolism.
View all proteins of this organism that are known to be involved in the pathway ether lipid biosynthesis and in Glycerolipid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei419 – 4191Important for enzyme activity
Binding sitei515 – 5151Substrate
Active sitei578 – 5781Proton donor/acceptor1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi234 – 2407FAD1 Publication
Nucleotide bindingi303 – 3097FAD1 Publication
Nucleotide bindingi316 – 3194FAD1 Publication
Nucleotide bindingi368 – 3747FAD1 Publication

GO - Molecular functioni

  • alkylglycerone-phosphate synthase activity Source: UniProtKB
  • FAD binding Source: UniProtKB
  • oxidoreductase activity, acting on CH-OH group of donors Source: InterPro

GO - Biological processi

  • ether lipid biosynthetic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

FAD, Flavoprotein

Enzyme and pathway databases

SABIO-RKP97275.
UniPathwayiUPA00781.

Chemistry

SwissLipidsiSLP:000000212.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkyldihydroxyacetonephosphate synthase, peroxisomal (EC:2.5.1.26)
Short name:
Alkyl-DHAP synthase
Alternative name(s):
Alkylglycerone-phosphate synthase
Gene namesi
Name:AGPS
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Peroxisome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi300 – 3001H → A: Loss of activity. 1 Publication
Mutagenesisi309 – 3091T → I: Impaired FAD binding and protein stability. Loss of activity. 1 Publication
Mutagenesisi367 – 3671S → A: Strongly reduced activity. 1 Publication
Mutagenesisi419 – 4191R → H: Loss of activity. 2 Publications
Mutagenesisi419 – 4191R → K: Strongly reduced activity. 2 Publications
Mutagenesisi469 – 4691L → P: Impaired FAD binding and protein stability. Loss of activity. 1 Publication
Mutagenesisi515 – 5151R → L: Impaired FAD binding and protein stability. Loss of activity. 1 Publication
Mutagenesisi578 – 5781Y → F: Loss of activity. 1 Publication
Mutagenesisi615 – 6151H → A: Loss of activity. 1 Publication
Mutagenesisi616 – 6161H → A: Loss of activity. 1 Publication
Mutagenesisi617 – 6171H → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5858Peroxisome1 PublicationAdd
BLAST
Chaini59 – 658600Alkyldihydroxyacetonephosphate synthase, peroxisomalPRO_0000020430Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei65 – 651PhosphoserineBy similarity
Modified residuei74 – 741PhosphothreonineBy similarity
Modified residuei102 – 1021N6-acetyllysineBy similarity
Modified residuei347 – 3471N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000000601.

Structurei

Secondary structure

1
658
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi87 – 893Combined sources
Beta strandi95 – 984Combined sources
Beta strandi103 – 1053Combined sources
Beta strandi111 – 1133Combined sources
Beta strandi115 – 1184Combined sources
Turni119 – 1224Combined sources
Helixi128 – 1369Combined sources
Beta strandi140 – 1423Combined sources
Helixi152 – 1543Combined sources
Helixi162 – 17110Combined sources
Beta strandi174 – 1763Combined sources
Helixi179 – 1846Combined sources
Helixi191 – 1988Combined sources
Beta strandi207 – 2115Combined sources
Helixi215 – 22713Combined sources
Beta strandi231 – 2377Combined sources
Beta strandi241 – 2433Combined sources
Beta strandi255 – 2595Combined sources
Beta strandi266 – 2705Combined sources
Turni271 – 2744Combined sources
Beta strandi275 – 2795Combined sources
Helixi284 – 29310Combined sources
Turni303 – 3075Combined sources
Helixi310 – 3167Combined sources
Helixi323 – 3264Combined sources
Helixi329 – 3324Combined sources
Beta strandi333 – 3408Combined sources
Beta strandi343 – 3464Combined sources
Beta strandi356 – 3583Combined sources
Helixi361 – 3644Combined sources
Beta strandi373 – 3808Combined sources
Beta strandi386 – 39712Combined sources
Helixi398 – 41013Combined sources
Beta strandi416 – 4216Combined sources
Helixi423 – 4319Combined sources
Beta strandi432 – 4343Combined sources
Helixi444 – 4496Combined sources
Turni451 – 4577Combined sources
Turni460 – 4623Combined sources
Beta strandi464 – 4729Combined sources
Helixi474 – 48916Combined sources
Turni490 – 4923Combined sources
Helixi498 – 52023Combined sources
Beta strandi523 – 53311Combined sources
Helixi534 – 5363Combined sources
Helixi537 – 55418Combined sources
Beta strandi562 – 5709Combined sources
Beta strandi572 – 58413Combined sources
Helixi591 – 60818Combined sources
Beta strandi615 – 6173Combined sources
Turni620 – 6223Combined sources
Helixi624 – 6263Combined sources
Helixi627 – 6315Combined sources
Helixi633 – 64614Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BBYX-ray1.90A/B/C/D1-658[»]
4BC7X-ray2.40A/B/C/D1-658[»]
4BC9X-ray2.41A/B/C/D1-658[»]
4BCAX-ray2.40A/B/C/D1-658[»]
5ADZX-ray2.20A/B/C/D1-658[»]
5AE1X-ray2.10A/B/C/D1-658[»]
5AE2X-ray2.00A/B/C/D1-658[»]
5AE3X-ray2.18A/B/C/D1-658[»]
ProteinModelPortaliP97275.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini202 – 384183FAD-binding PCMH-typePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni615 – 6173Important for enzyme activity

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi2 – 1413Poly-AlaAdd
BLAST

Sequence similaritiesi

Contains 1 FAD-binding PCMH-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1233. Eukaryota.
COG0277. LUCA.
GeneTreeiENSGT00530000063515.
HOGENOMiHOG000231620.
HOVERGENiHBG004179.
InParanoidiP97275.
OMAiEIMKWNG.
OrthoDBiEOG091G07KJ.
TreeFamiTF313830.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR025650. Alkyl-DHAP_Synthase.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR004113. FAD-linked_oxidase_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR11748:SF3. PTHR11748:SF3. 1 hit.
PfamiPF02913. FAD-oxidase_C. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 2 hits.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P97275-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAEAAAAAAA AAAAGETSAS SGSAAERDPD QDRAGRRLRV LSGHLLGRPQ
60 70 80 90 100
EALSTNECKA RRAASAATAA PTATPAAPES GIIPKKRQEL MKWNGWGYND
110 120 130 140 150
SKFFLNKKGQ LELTGKRYPL SGVALPTFKD WIQNTFGINL DHKTTSKASL
160 170 180 190 200
NPSDTPPSIV NEDFLHELKK TNISYSQEAD DRVFRAHGHC LHEIFLLREG
210 220 230 240 250
MFERIPDIVL WPTCHDDVVK IVNLACKYNL CIIPIGGGTS VSYGLMCPAD
260 270 280 290 300
ETRTIISLDT SQMNRILWVD ENNLTAHVEA GITGQELERQ LKESGYCTGH
310 320 330 340 350
EPDSLEFSTV GGWISTRASG MKKNIYGNIE DLVVHMKVVT PRGVIEKSCQ
360 370 380 390 400
GPRMSTGPDI HHFIMGSEGT LGVITEATIK IRPTPEYQKY GSVAFPNFEQ
410 420 430 440 450
GVACLREIAK QRCAPASIRL MDNQQFQFGH ALKPQVSSIF TSFLDGLKKF
460 470 480 490 500
YITKFKGFDP NQLSVATLLF EGDREKVLQH EKQVYDIAAK FGGLAAGEDN
510 520 530 540 550
GQRGYLLTYV IAYMRDLGLE YYIIGESFET SAPWDRVVDL CRNVKERIRR
560 570 580 590 600
ECKEKGVQFP PLSTCRVTQT YDAGACIYFY FAFNYRGISD PLAVFEQTEA
610 620 630 640 650
AAREEILANG GSLSHHHGVG KLRKQWLKES ISDVGFGMLK SVKDYVDPTN

IFGNRNLL
Length:658
Mass (Da):72,846
Last modified:May 1, 1997 - v1
Checksum:iB6272EB407EBB5F1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08826 mRNA. Translation: CAA70060.1.
RefSeqiXP_005007711.2. XM_005007654.2.

Genome annotation databases

EnsembliENSCPOT00000000684; ENSCPOP00000000601; ENSCPOG00000000679.
GeneIDi100734021.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y08826 mRNA. Translation: CAA70060.1.
RefSeqiXP_005007711.2. XM_005007654.2.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4BBYX-ray1.90A/B/C/D1-658[»]
4BC7X-ray2.40A/B/C/D1-658[»]
4BC9X-ray2.41A/B/C/D1-658[»]
4BCAX-ray2.40A/B/C/D1-658[»]
5ADZX-ray2.20A/B/C/D1-658[»]
5AE1X-ray2.10A/B/C/D1-658[»]
5AE2X-ray2.00A/B/C/D1-658[»]
5AE3X-ray2.18A/B/C/D1-658[»]
ProteinModelPortaliP97275.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000000601.

Chemistry

SwissLipidsiSLP:000000212.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSCPOT00000000684; ENSCPOP00000000601; ENSCPOG00000000679.
GeneIDi100734021.

Organism-specific databases

CTDi8540.

Phylogenomic databases

eggNOGiKOG1233. Eukaryota.
COG0277. LUCA.
GeneTreeiENSGT00530000063515.
HOGENOMiHOG000231620.
HOVERGENiHBG004179.
InParanoidiP97275.
OMAiEIMKWNG.
OrthoDBiEOG091G07KJ.
TreeFamiTF313830.

Enzyme and pathway databases

UniPathwayiUPA00781.
SABIO-RKP97275.

Family and domain databases

Gene3Di3.30.43.10. 1 hit.
3.30.465.10. 1 hit.
InterProiIPR025650. Alkyl-DHAP_Synthase.
IPR016169. CO_DH_flavot_FAD-bd_sub2.
IPR016166. FAD-bd_2.
IPR016167. FAD-bd_2_sub1.
IPR016164. FAD-linked_Oxase-like_C.
IPR004113. FAD-linked_oxidase_C.
IPR006094. Oxid_FAD_bind_N.
[Graphical view]
PANTHERiPTHR11748:SF3. PTHR11748:SF3. 1 hit.
PfamiPF02913. FAD-oxidase_C. 1 hit.
PF01565. FAD_binding_4. 1 hit.
[Graphical view]
SUPFAMiSSF55103. SSF55103. 2 hits.
SSF56176. SSF56176. 1 hit.
PROSITEiPS51387. FAD_PCMH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiADAS_CAVPO
AccessioniPrimary (citable) accession number: P97275
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: May 1, 1997
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.