Reviewed, UniProtKB/Swiss-Prot P97270 (NOS3_CAVPO)

Names and origin

Protein names

Recommended name:
    Nitric oxide synthase, endothelial
    EC=1.14.13.39
Alternative name(s):
    Endothelial NOS
      Short name=eNOS
    EC-NOS
    NOS type III
      Short name=NOSIII
    Constitutive NOS
      Short name=cNOS

Gene names

Name:	NOS3
Synonyms:	ENOS

Organism

Cavia porcellus (Guinea pig)

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	100 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets By similarity.
Catalytic activity	L-arginine + n NADPH + m O(2) = citrulline + nitric oxide + n NADP(+).
Cofactor	Heme group By similarity. Binds 1 FAD By similarity. Binds 1 FMN By similarity. Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.
Enzyme regulation	Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN By similarity.
Subunit structure	Homodimer. Interacts with NOSIP and NOSTRIN By similarity.
Subcellular location	Cell membrane › caveolaBy similarity. Cytoplasm › cytoskeletonBy similarity. Golgi apparatusBy similarity. Note= Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity By similarity.
Induction	Repressed by hypoxemia in fetal brain.
Sequence similarities	Belongs to the NOS family.

Ontologies

Keywords
Cellular component	Cell membrane Cytoplasm Cytoskeleton Golgi apparatus Membrane
Ligand	Calcium Calmodulin-binding FAD FMN Heme Iron Metal-binding NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	nitric oxide biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW nitric-oxide synthase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›100	›100	Nitric oxide synthase, endothelial		PRO_0000170942
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	100	1

Sequences

	Sequence		Length	Mass (Da)
	P97270-1 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 867D7F89F4B132A8	FASTA	100	11,271
	10 20 30 40 50 60 WYMSTEIGTR NLCDPHRYNI LEDVAVCMDL DTRTTSSLWK DKAAVEINVA VLHSYQLAKV 70 80 90 100 TIVDHHAATV SFMKHLENEQ KGRGGCPADW GWDPCPPSRA

References

[1]	"Effect of chronic hypoxemia on the regulation of nitric-oxide synthase in the fetal sheep brain." Aguan K., Murotsuki J., Gagnon R., Thompson L.P., Weiner C.P. Brain Res. Dev. Brain Res. 111:271-277(1998) [PubMed: 9838160] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Endothelial cell.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U76736 mRNA. Translation: AAB40703.1.
3D structure databases
HSSP	HSSP built from PDB template 1M9M based on UniProtKB P29474.
SMR	P97270. Positions 1-98.
ModBase	Search...
Phylogenomic databases
HOVERGEN	P97270.
Family and domain databases
InterPro	IPR004030. NO_synthase_oxygenase_reg. [Graphical view]
Pfam	PF02898. NO_synthase. 1 hit. [Graphical view]
PROSITE	PS60001. NOS. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOS3_CAVPO

Accession

Primary (citable) accession number: P97270

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 1, 1997
Last modified:	November 25, 2008
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)