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P97270

- NOS3_CAVPO

UniProt

P97270 - NOS3_CAVPO

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Protein

Nitric oxide synthase, endothelial

Gene

NOS3

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets By similarity.By similarity

Catalytic activityi

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactori

Heme group.By similarity
Binds 1 FAD.By similarity
Binds 1 FMN.By similarity
Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.By similarity

Enzyme regulationi

Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN By similarity.By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitric-oxide synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitric oxide biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Calmodulin-binding, FAD, Flavoprotein, FMN, Heme, Iron, Metal-binding, NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Nitric oxide synthase, endothelial (EC:1.14.13.39)
Alternative name(s):
Constitutive NOS
Short name:
cNOS
EC-NOS
Endothelial NOS
Short name:
eNOS
NOS type III
Short name:
NOSIII
Gene namesi
Name:NOS3
Synonyms:ENOS
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

Subcellular locationi

Membranecaveola By similarity. Cytoplasmcytoskeleton By similarity. Golgi apparatus By similarity. Cell membrane By similarity
Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity.By similarity

GO - Cellular componenti

  1. cytoskeleton Source: UniProtKB-KW
  2. Golgi apparatus Source: UniProtKB-KW
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini‹1 – ›100›100Nitric oxide synthase, endothelialPRO_0000170942Add
BLAST

Expressioni

Inductioni

Repressed by hypoxemia in fetal brain.

Interactioni

Subunit structurei

Homodimer. Interacts with NOSIP and NOSTRIN By similarity.By similarity

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000003945.

Structurei

3D structure databases

ProteinModelPortaliP97270.
SMRiP97270. Positions 1-98.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NOS family.Curated

Phylogenomic databases

eggNOGiCOG4362.
InParanoidiP97270.

Family and domain databases

InterProiIPR004030. NOS_N.
[Graphical view]
PfamiPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF56512. SSF56512. 1 hit.

Sequencei

Sequence statusi: Fragment.

P97270-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
WYMSTEIGTR NLCDPHRYNI LEDVAVCMDL DTRTTSSLWK DKAAVEINVA
60 70 80 90 100
VLHSYQLAKV TIVDHHAATV SFMKHLENEQ KGRGGCPADW GWDPCPPSRA
Length:100
Mass (Da):11,271
Last modified:May 1, 1997 - v1
Checksum:i867D7F89F4B132A8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Non-terminal residuei100 – 1001

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U76736 mRNA. Translation: AAB40703.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U76736 mRNA. Translation: AAB40703.1 .

3D structure databases

ProteinModelPortali P97270.
SMRi P97270. Positions 1-98.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000003945.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

eggNOGi COG4362.
InParanoidi P97270.

Family and domain databases

InterProi IPR004030. NOS_N.
[Graphical view ]
Pfami PF02898. NO_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF56512. SSF56512. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Effect of chronic hypoxemia on the regulation of nitric-oxide synthase in the fetal sheep brain."
    Aguan K., Murotsuki J., Gagnon R., Thompson L.P., Weiner C.P.
    Brain Res. Dev. Brain Res. 111:271-277(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Endothelial cell.

Entry informationi

Entry nameiNOS3_CAVPO
AccessioniPrimary (citable) accession number: P97270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: October 29, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3