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Reviewed, UniProtKB/Swiss-Prot P97270 (NOS3_CAVPO)

Last modified January 19, 2010. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Nitric oxide synthase, endothelial
    EC=1.14.13.39
Alternative name(s):
    Endothelial NOS
      Short name=eNOS
    EC-NOS
    NOS type III
      Short name=NOSIII
    Constitutive NOS
      Short name=cNOS
Gene names
Name: NOS3
Synonyms: ENOS
OrganismCavia porcellus (Guinea pig)
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length100 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets By similarity.

Catalytic activity

L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN By similarity.

Subunit structure

Homodimer. Interacts with NOSIP and NOSTRIN By similarity.

Subcellular location

Membranecaveola By similarity. Cytoplasmcytoskeleton By similarity. Golgi apparatus By similarity. Cell membrane By similarity. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity By similarity.

Induction

Repressed by hypoxemia in fetal brain.

Sequence similarities

Belongs to the NOS family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›100›100Nitric oxide synthase, endothelial
PRO_0000170942

Experimental info

Non-terminal residue11
Non-terminal residue1001

Sequences

Sequence LengthMass (Da)Tools
P97270-1 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 867D7F89F4B132A8

FASTA10011,271
        10         20         30         40         50         60 
WYMSTEIGTR NLCDPHRYNI LEDVAVCMDL DTRTTSSLWK DKAAVEINVA VLHSYQLAKV 

        70         80         90        100 
TIVDHHAATV SFMKHLENEQ KGRGGCPADW GWDPCPPSRA 

« Hide

References

[1]"Effect of chronic hypoxemia on the regulation of nitric-oxide synthase in the fetal sheep brain."
Aguan K., Murotsuki J., Gagnon R., Thompson L.P., Weiner C.P.
Brain Res. Dev. Brain Res. 111:271-277(1998) [PubMed: 9838160] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Endothelial cell.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U76736 mRNA. Translation: AAB40703.1.

3D structure databases

SMRP97270. Positions 1-98.
ModBaseSearch...

Genome annotation databases

EnsemblENSCPOT00000026637; ENSCPOP00000017885; ENSCPOG00000004373; Cavia porcellus. [Genome view]

Phylogenomic databases

HOVERGENP97270.

Enzyme and pathway databases

BRENDA1.14.13.39. 44.

Family and domain databases

InterProIPR004030. NO_synthase_oxygenase_dom.
[Graphical view]
PfamPF02898. NO_synthase. 1 hit.
[Graphical view]
PROSITEPS60001. NOS. Partial match.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNOS3_CAVPO
AccessionPrimary (citable) accession number: P97270
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: January 19, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents