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P97270 (NOS3_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitric oxide synthase, endothelial

EC=1.14.13.39
Alternative name(s):
Constitutive NOS
Short name=cNOS
EC-NOS
Endothelial NOS
Short name=eNOS
NOS type III
Short name=NOSIII
Gene names
Name:NOS3
Synonyms:ENOS
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length100 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets By similarity.

Catalytic activity

2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor

Heme group By similarity.

Binds 1 FAD By similarity.

Binds 1 FMN By similarity.

Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme By similarity.

Enzyme regulation

Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN By similarity.

Subunit structure

Homodimer. Interacts with NOSIP and NOSTRIN By similarity.

Subcellular location

Membranecaveola By similarity. Cytoplasmcytoskeleton By similarity. Golgi apparatus By similarity. Cell membrane By similarity. Note: Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle, which is favored by interaction with NOSIP and results in a reduced enzymatic activity By similarity.

Induction

Repressed by hypoxemia in fetal brain.

Sequence similarities

Belongs to the NOS family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – ›100›100Nitric oxide synthase, endothelial
PRO_0000170942

Experimental info

Non-terminal residue11
Non-terminal residue1001

Sequences

Sequence LengthMass (Da)Tools
P97270 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 867D7F89F4B132A8

FASTA10011,271
        10         20         30         40         50         60 
WYMSTEIGTR NLCDPHRYNI LEDVAVCMDL DTRTTSSLWK DKAAVEINVA VLHSYQLAKV 

        70         80         90        100 
TIVDHHAATV SFMKHLENEQ KGRGGCPADW GWDPCPPSRA 

« Hide

References

[1]"Effect of chronic hypoxemia on the regulation of nitric-oxide synthase in the fetal sheep brain."
Aguan K., Murotsuki J., Gagnon R., Thompson L.P., Weiner C.P.
Brain Res. Dev. Brain Res. 111:271-277(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Endothelial cell.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U76736 mRNA. Translation: AAB40703.1.

3D structure databases

ProteinModelPortalP97270.
SMRP97270. Positions 1-98.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000003945.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG4362.

Family and domain databases

InterProIPR004030. NO_synthase_oxygenase_dom.
[Graphical view]
PfamPF02898. NO_synthase. 1 hit.
[Graphical view]
SUPFAMSSF56512. SSF56512. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNOS3_CAVPO
AccessionPrimary (citable) accession number: P97270
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families