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P97265

- GBA3_CAVPO

UniProt

P97265 - GBA3_CAVPO

Protein

Cytosolic beta-glucosidase

Gene

Gba3

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 May 1997)
      Previous versions | rss
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    Functioni

    Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity.By similarity

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

    Enzyme regulationi

    Inhibited by 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei17 – 171SubstrateBy similarity
    Binding sitei120 – 1201SubstrateBy similarity
    Binding sitei164 – 1641SubstrateBy similarity
    Active sitei165 – 1651Proton donorSequence Analysis
    Binding sitei309 – 3091SubstrateBy similarity
    Active sitei373 – 3731NucleophileSequence Analysis
    Binding sitei417 – 4171SubstrateBy similarity

    GO - Molecular functioni

    1. beta-galactosidase activity Source: UniProtKB
    2. beta-glucosidase activity Source: UniProtKB
    3. glycosylceramidase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. glycosylceramide catabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Protein family/group databases

    CAZyiGH1. Glycoside Hydrolase Family 1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cytosolic beta-glucosidase (EC:3.2.1.21)
    Gene namesi
    Name:Gba3
    Synonyms:Cbg
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    Subcellular locationi

    Cytoplasmcytosol 1 Publication

    GO - Cellular componenti

    1. cytosol Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 469469Cytosolic beta-glucosidasePRO_0000063907Add
    BLAST

    Post-translational modificationi

    The N-terminus is blocked.

    Proteomic databases

    PRIDEiP97265.

    Expressioni

    Tissue specificityi

    Present in hepatocytes (at protein level).1 Publication

    Interactioni

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000014554.

    Structurei

    3D structure databases

    ProteinModelPortaliP97265.
    SMRiP97265. Positions 1-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni424 – 4252Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2723.
    HOGENOMiHOG000088630.
    HOVERGENiHBG053101.
    InParanoidiP97265.

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PANTHERiPTHR10353. PTHR10353. 1 hit.
    PfamiPF00232. Glyco_hydro_1. 1 hit.
    [Graphical view]
    PRINTSiPR00131. GLHYDRLASE1.
    SUPFAMiSSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P97265-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFPADLVGG LPTAAYQVEG GWDADGRGPC VWDTFTHQGG ERVFKNQTGD    50
    VACGSYTLWE EDLKCIKQLG LTHYRFSISW SRLLPDGTTG FINQKGVDYY 100
    NKIIDDLLTN GVTPVVTLYH FDLPQALEDQ GGWLSEAIIE VFDKYAQFCF 150
    STFGNRVRQW ITINEPNVLC AMGYDLGFFA PGVSQIGTGG YQAAHNMIKA 200
    HARAWHSYDS LFREKQKGMV SLSLFCIWPQ PENPNSVLDQ KAAERAINFQ 250
    FDFFAKPIFI DGDYPELVKS QIASMSEKQG YPSSRLSKFT EEEKKMIKGT 300
    ADFFAVQYYT TRFIRHKENK EAELGILQDA EIELFSDPSW KGVGWVRVVP 350
    WGIRKLLNYI KDTYNNPVIY ITENGFPQDD PPSIDDTQRW ECFRQTFEEL 400
    FKAIHVDKVN LQLYCAWSLL DNFEWNDGYS KRFGLFHVDF EDPAKPRVPY 450
    TSAKEYAKII RNNGLERPQ 469
    Length:469
    Mass (Da):53,745
    Last modified:May 1, 1997 - v1
    Checksum:i9ED6B5BA89C22EE6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U50545 mRNA. Translation: AAB41058.1.
    RefSeqiNP_001166590.1. NM_001173119.1.

    Genome annotation databases

    GeneIDi100379247.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U50545 mRNA. Translation: AAB41058.1 .
    RefSeqi NP_001166590.1. NM_001173119.1.

    3D structure databases

    ProteinModelPortali P97265.
    SMRi P97265. Positions 1-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000014554.

    Protein family/group databases

    CAZyi GH1. Glycoside Hydrolase Family 1.

    Proteomic databases

    PRIDEi P97265.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100379247.

    Organism-specific databases

    CTDi 57733.

    Phylogenomic databases

    eggNOGi COG2723.
    HOGENOMi HOG000088630.
    HOVERGENi HBG053101.
    InParanoidi P97265.

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR001360. Glyco_hydro_1.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    PANTHERi PTHR10353. PTHR10353. 1 hit.
    Pfami PF00232. Glyco_hydro_1. 1 hit.
    [Graphical view ]
    PRINTSi PR00131. GLHYDRLASE1.
    SUPFAMi SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the cytosolic beta-glucosidase of guinea pig liver."
      Hays W.S., Jenison S.A., Yamada T., Pastuszyn A., Glew R.H.
      Biochem. J. 319:829-837(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-41; 46-59; 76-82; 96-102; 204-212; 218-232; 246-260; 270-274; 299-310; 321-335; 390-394 AND 448-454, BLOCKAGE OF N-TERMINUS.
      Strain: Dunkin-Hartley.
      Tissue: Liver.
    2. "Expression of cytosolic beta-glucosidase in guinea pig liver cells."
      Hays W.S., Wheeler D.E., Eghtesad B., Glew R.H., Johnston D.E.
      Hepatology 28:156-163(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ENZYME REGULATION.

    Entry informationi

    Entry nameiGBA3_CAVPO
    AccessioniPrimary (citable) accession number: P97265
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 11, 2005
    Last sequence update: May 1, 1997
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3