P97265 (GBA3_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytosolic beta-glucosidase
EC=3.2.1.21

Gene names

Name:	Gba3
Synonyms:	Cbg

Organism

Cavia porcellus (Guinea pig) [Reference proteome]

Taxonomic identifier

10141 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Hystricognathi › Caviidae › Cavia

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity. Ref.2
Catalytic activity	Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.
Enzyme regulation	Inhibited by 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside. Ref.2
Subcellular location	Cytoplasm › cytosol Ref.2.
Tissue specificity	Present in hepatocytes (at protein level). Ref.2
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycosylceramide catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	cytosol Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	beta-galactosidase activity Inferred from sequence or structural similarity. Source: UniProtKB beta-glucosidase activity Inferred from sequence or structural similarity. Source: UniProtKB glycosylceramidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	Cytosolic beta-glucosidase		PRO_0000063907
Regions
	Region	424 – 425	2	Substrate binding By similarity
Sites
	Active site	165	1	Proton donor Potential
	Active site	373	1	Nucleophile Potential
	Binding site	17	1	Substrate By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	309	1	Substrate By similarity
	Binding site	417	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P97265 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: 9ED6B5BA89C22EE6	FASTA	469	53,745
	10 20 30 40 50 60 MAFPADLVGG LPTAAYQVEG GWDADGRGPC VWDTFTHQGG ERVFKNQTGD VACGSYTLWE 70 80 90 100 110 120 EDLKCIKQLG LTHYRFSISW SRLLPDGTTG FINQKGVDYY NKIIDDLLTN GVTPVVTLYH 130 140 150 160 170 180 FDLPQALEDQ GGWLSEAIIE VFDKYAQFCF STFGNRVRQW ITINEPNVLC AMGYDLGFFA 190 200 210 220 230 240 PGVSQIGTGG YQAAHNMIKA HARAWHSYDS LFREKQKGMV SLSLFCIWPQ PENPNSVLDQ 250 260 270 280 290 300 KAAERAINFQ FDFFAKPIFI DGDYPELVKS QIASMSEKQG YPSSRLSKFT EEEKKMIKGT 310 320 330 340 350 360 ADFFAVQYYT TRFIRHKENK EAELGILQDA EIELFSDPSW KGVGWVRVVP WGIRKLLNYI 370 380 390 400 410 420 KDTYNNPVIY ITENGFPQDD PPSIDDTQRW ECFRQTFEEL FKAIHVDKVN LQLYCAWSLL 430 440 450 460 DNFEWNDGYS KRFGLFHVDF EDPAKPRVPY TSAKEYAKII RNNGLERPQ

References

[1]

"Primary structure of the cytosolic beta-glucosidase of guinea pig liver." Hays W.S., Jenison S.A., Yamada T., Pastuszyn A., Glew R.H. Biochem. J. 319:829-837(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-41; 46-59; 76-82; 96-102; 204-212; 218-232; 246-260; 270-274; 299-310; 321-335; 390-394 AND 448-454, BLOCKAGE OF N-TERMINUS. Strain: Dunkin-Hartley. Tissue: Liver.

[2]

"Expression of cytosolic beta-glucosidase in guinea pig liver cells." Hays W.S., Wheeler D.E., Eghtesad B., Glew R.H., Johnston D.E. Hepatology 28:156-163(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ENZYME REGULATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U50545 mRNA. Translation: AAB41058.1.
RefSeq	NP_001166590.1. NM_001173119.1.
3D structure databases
ProteinModelPortal	P97265.
SMR	P97265. Positions 1-466.
ModBase	Search...
Protein-protein interaction databases
STRING	10141.ENSCPOP00000014554.
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Proteomic databases
PRIDE	P97265.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100379247.
Organism-specific databases
CTD	57733.
Phylogenomic databases
eggNOG	COG2723.
HOGENOM	HOG000088630.
HOVERGEN	HBG053101.
InParanoid	P97265.
OrthoDB	EOG4WDDBM.
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR001360. Glyco_hydro_1. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
PANTHER	PTHR10353. PTHR10353. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. False negative. PS00653. GLYCOSYL_HYDROL_F1_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GBA3_CAVPO

Accession

Primary (citable) accession number: P97265

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 11, 2005
Last sequence update:	May 1, 1997
Last modified:	May 1, 2013
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program