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P97265 (GBA3_CAVPO) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cytosolic beta-glucosidase

EC=3.2.1.21
Gene names
Name:Gba3
Synonyms:Cbg
OrganismCavia porcellus (Guinea pig) [Reference proteome]
Taxonomic identifier10141 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia

Protein attributes

Sequence length469 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity. Ref.2

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Enzyme regulation

Inhibited by 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside. Ref.2

Subcellular location

Cytoplasmcytosol Ref.2.

Tissue specificity

Present in hepatocytes (at protein level). Ref.2

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the glycosyl hydrolase 1 family. Klotho subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 469469Cytosolic beta-glucosidase
PRO_0000063907

Regions

Region424 – 4252Substrate binding By similarity

Sites

Active site1651Proton donor Potential
Active site3731Nucleophile Potential
Binding site171Substrate By similarity
Binding site1201Substrate By similarity
Binding site1641Substrate By similarity
Binding site3091Substrate By similarity
Binding site4171Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P97265 [UniParc].

Last modified May 1, 1997. Version 1.
Checksum: 9ED6B5BA89C22EE6

FASTA46953,745
        10         20         30         40         50         60 
MAFPADLVGG LPTAAYQVEG GWDADGRGPC VWDTFTHQGG ERVFKNQTGD VACGSYTLWE 

        70         80         90        100        110        120 
EDLKCIKQLG LTHYRFSISW SRLLPDGTTG FINQKGVDYY NKIIDDLLTN GVTPVVTLYH 

       130        140        150        160        170        180 
FDLPQALEDQ GGWLSEAIIE VFDKYAQFCF STFGNRVRQW ITINEPNVLC AMGYDLGFFA 

       190        200        210        220        230        240 
PGVSQIGTGG YQAAHNMIKA HARAWHSYDS LFREKQKGMV SLSLFCIWPQ PENPNSVLDQ 

       250        260        270        280        290        300 
KAAERAINFQ FDFFAKPIFI DGDYPELVKS QIASMSEKQG YPSSRLSKFT EEEKKMIKGT 

       310        320        330        340        350        360 
ADFFAVQYYT TRFIRHKENK EAELGILQDA EIELFSDPSW KGVGWVRVVP WGIRKLLNYI 

       370        380        390        400        410        420 
KDTYNNPVIY ITENGFPQDD PPSIDDTQRW ECFRQTFEEL FKAIHVDKVN LQLYCAWSLL 

       430        440        450        460 
DNFEWNDGYS KRFGLFHVDF EDPAKPRVPY TSAKEYAKII RNNGLERPQ 

« Hide

References

[1]"Primary structure of the cytosolic beta-glucosidase of guinea pig liver."
Hays W.S., Jenison S.A., Yamada T., Pastuszyn A., Glew R.H.
Biochem. J. 319:829-837(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-41; 46-59; 76-82; 96-102; 204-212; 218-232; 246-260; 270-274; 299-310; 321-335; 390-394 AND 448-454, BLOCKAGE OF N-TERMINUS.
Strain: Dunkin-Hartley.
Tissue: Liver.
[2]"Expression of cytosolic beta-glucosidase in guinea pig liver cells."
Hays W.S., Wheeler D.E., Eghtesad B., Glew R.H., Johnston D.E.
Hepatology 28:156-163(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ENZYME REGULATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U50545 mRNA. Translation: AAB41058.1.
RefSeqNP_001166590.1. NM_001173119.1.

3D structure databases

ProteinModelPortalP97265.
SMRP97265. Positions 1-466.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10141.ENSCPOP00000014554.

Protein family/group databases

CAZyGH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEP97265.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100379247.

Organism-specific databases

CTD57733.

Phylogenomic databases

eggNOGCOG2723.
HOGENOMHOG000088630.
HOVERGENHBG053101.
InParanoidP97265.

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10353. PTHR10353. 1 hit.
PfamPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSPR00131. GLHYDRLASE1.
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGBA3_CAVPO
AccessionPrimary (citable) accession number: P97265
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries