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P97265

- GBA3_CAVPO

UniProt

P97265 - GBA3_CAVPO

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Protein
Cytosolic beta-glucosidase
Gene
Gba3, Cbg
Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity.1 Publication

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Enzyme regulationi

Inhibited by 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171Substrate By similarity
Binding sitei120 – 1201Substrate By similarity
Binding sitei164 – 1641Substrate By similarity
Active sitei165 – 1651Proton donor Reviewed prediction
Binding sitei309 – 3091Substrate By similarity
Active sitei373 – 3731Nucleophile Reviewed prediction
Binding sitei417 – 4171Substrate By similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB
  2. beta-glucosidase activity Source: UniProtKB
  3. glycosylceramidase activity Source: UniProtKB
Complete GO annotation...

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycosylceramide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic beta-glucosidase (EC:3.2.1.21)
Gene namesi
Name:Gba3
Synonyms:Cbg
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

Subcellular locationi

Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Cytosolic beta-glucosidase
PRO_0000063907Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiP97265.

Expressioni

Tissue specificityi

Present in hepatocytes (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000014554.

Structurei

3D structure databases

ProteinModelPortaliP97265.
SMRiP97265. Positions 1-466.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni424 – 4252Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
HOVERGENiHBG053101.
InParanoidiP97265.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P97265-1 [UniParc]FASTAAdd to Basket

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MAFPADLVGG LPTAAYQVEG GWDADGRGPC VWDTFTHQGG ERVFKNQTGD    50
VACGSYTLWE EDLKCIKQLG LTHYRFSISW SRLLPDGTTG FINQKGVDYY 100
NKIIDDLLTN GVTPVVTLYH FDLPQALEDQ GGWLSEAIIE VFDKYAQFCF 150
STFGNRVRQW ITINEPNVLC AMGYDLGFFA PGVSQIGTGG YQAAHNMIKA 200
HARAWHSYDS LFREKQKGMV SLSLFCIWPQ PENPNSVLDQ KAAERAINFQ 250
FDFFAKPIFI DGDYPELVKS QIASMSEKQG YPSSRLSKFT EEEKKMIKGT 300
ADFFAVQYYT TRFIRHKENK EAELGILQDA EIELFSDPSW KGVGWVRVVP 350
WGIRKLLNYI KDTYNNPVIY ITENGFPQDD PPSIDDTQRW ECFRQTFEEL 400
FKAIHVDKVN LQLYCAWSLL DNFEWNDGYS KRFGLFHVDF EDPAKPRVPY 450
TSAKEYAKII RNNGLERPQ 469
Length:469
Mass (Da):53,745
Last modified:May 1, 1997 - v1
Checksum:i9ED6B5BA89C22EE6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U50545 mRNA. Translation: AAB41058.1.
RefSeqiNP_001166590.1. NM_001173119.1.

Genome annotation databases

GeneIDi100379247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U50545 mRNA. Translation: AAB41058.1 .
RefSeqi NP_001166590.1. NM_001173119.1.

3D structure databases

ProteinModelPortali P97265.
SMRi P97265. Positions 1-466.
ModBasei Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000014554.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi P97265.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100379247.

Organism-specific databases

CTDi 57733.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
HOVERGENi HBG053101.
InParanoidi P97265.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Primary structure of the cytosolic beta-glucosidase of guinea pig liver."
    Hays W.S., Jenison S.A., Yamada T., Pastuszyn A., Glew R.H.
    Biochem. J. 319:829-837(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-41; 46-59; 76-82; 96-102; 204-212; 218-232; 246-260; 270-274; 299-310; 321-335; 390-394 AND 448-454, BLOCKAGE OF N-TERMINUS.
    Strain: Dunkin-Hartley.
    Tissue: Liver.
  2. "Expression of cytosolic beta-glucosidase in guinea pig liver cells."
    Hays W.S., Wheeler D.E., Eghtesad B., Glew R.H., Johnston D.E.
    Hepatology 28:156-163(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ENZYME REGULATION.

Entry informationi

Entry nameiGBA3_CAVPO
AccessioniPrimary (citable) accession number: P97265
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: May 1, 1997
Last modified: February 19, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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