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P97265

- GBA3_CAVPO

UniProt

P97265 - GBA3_CAVPO

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Protein

Cytosolic beta-glucosidase

Gene

Gba3

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Seems to be involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. Able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. Possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide By similarity.By similarity

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose.

Enzyme regulationi

Inhibited by 2,4-dinitrophenyl-2-fluoro-2-deoxy-beta-D-glucopyranoside.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171SubstrateBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Binding sitei164 – 1641SubstrateBy similarity
Active sitei165 – 1651Proton donorSequence Analysis
Binding sitei309 – 3091SubstrateBy similarity
Active sitei373 – 3731NucleophileSequence Analysis
Binding sitei417 – 4171SubstrateBy similarity

GO - Molecular functioni

  1. beta-galactosidase activity Source: UniProtKB
  2. beta-glucosidase activity Source: UniProtKB
  3. glycosylceramidase activity Source: UniProtKB

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. glycosylceramide catabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH1. Glycoside Hydrolase Family 1.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosolic beta-glucosidase (EC:3.2.1.21)
Gene namesi
Name:Gba3
Synonyms:Cbg
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

Subcellular locationi

Cytoplasmcytosol 1 Publication

GO - Cellular componenti

  1. cytosol Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Cytosolic beta-glucosidasePRO_0000063907Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PRIDEiP97265.

Expressioni

Tissue specificityi

Present in hepatocytes (at protein level).1 Publication

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000014554.

Structurei

3D structure databases

ProteinModelPortaliP97265.
SMRiP97265. Positions 1-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni424 – 4252Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2723.
HOGENOMiHOG000088630.
HOVERGENiHBG053101.
InParanoidiP97265.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERiPTHR10353. PTHR10353. 1 hit.
PfamiPF00232. Glyco_hydro_1. 1 hit.
[Graphical view]
PRINTSiPR00131. GLHYDRLASE1.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

P97265-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFPADLVGG LPTAAYQVEG GWDADGRGPC VWDTFTHQGG ERVFKNQTGD
60 70 80 90 100
VACGSYTLWE EDLKCIKQLG LTHYRFSISW SRLLPDGTTG FINQKGVDYY
110 120 130 140 150
NKIIDDLLTN GVTPVVTLYH FDLPQALEDQ GGWLSEAIIE VFDKYAQFCF
160 170 180 190 200
STFGNRVRQW ITINEPNVLC AMGYDLGFFA PGVSQIGTGG YQAAHNMIKA
210 220 230 240 250
HARAWHSYDS LFREKQKGMV SLSLFCIWPQ PENPNSVLDQ KAAERAINFQ
260 270 280 290 300
FDFFAKPIFI DGDYPELVKS QIASMSEKQG YPSSRLSKFT EEEKKMIKGT
310 320 330 340 350
ADFFAVQYYT TRFIRHKENK EAELGILQDA EIELFSDPSW KGVGWVRVVP
360 370 380 390 400
WGIRKLLNYI KDTYNNPVIY ITENGFPQDD PPSIDDTQRW ECFRQTFEEL
410 420 430 440 450
FKAIHVDKVN LQLYCAWSLL DNFEWNDGYS KRFGLFHVDF EDPAKPRVPY
460
TSAKEYAKII RNNGLERPQ
Length:469
Mass (Da):53,745
Last modified:May 1, 1997 - v1
Checksum:i9ED6B5BA89C22EE6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U50545 mRNA. Translation: AAB41058.1.
RefSeqiNP_001166590.1. NM_001173119.1.

Genome annotation databases

GeneIDi100379247.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U50545 mRNA. Translation: AAB41058.1 .
RefSeqi NP_001166590.1. NM_001173119.1.

3D structure databases

ProteinModelPortali P97265.
SMRi P97265. Positions 1-466.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000014554.

Protein family/group databases

CAZyi GH1. Glycoside Hydrolase Family 1.

Proteomic databases

PRIDEi P97265.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100379247.

Organism-specific databases

CTDi 57733.

Phylogenomic databases

eggNOGi COG2723.
HOGENOMi HOG000088630.
HOVERGENi HBG053101.
InParanoidi P97265.

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR001360. Glyco_hydro_1.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
PANTHERi PTHR10353. PTHR10353. 1 hit.
Pfami PF00232. Glyco_hydro_1. 1 hit.
[Graphical view ]
PRINTSi PR00131. GLHYDRLASE1.
SUPFAMi SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Primary structure of the cytosolic beta-glucosidase of guinea pig liver."
    Hays W.S., Jenison S.A., Yamada T., Pastuszyn A., Glew R.H.
    Biochem. J. 319:829-837(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 28-41; 46-59; 76-82; 96-102; 204-212; 218-232; 246-260; 270-274; 299-310; 321-335; 390-394 AND 448-454, BLOCKAGE OF N-TERMINUS.
    Strain: Dunkin-Hartley.
    Tissue: Liver.
  2. "Expression of cytosolic beta-glucosidase in guinea pig liver cells."
    Hays W.S., Wheeler D.E., Eghtesad B., Glew R.H., Johnston D.E.
    Hepatology 28:156-163(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, ENZYME REGULATION.

Entry informationi

Entry nameiGBA3_CAVPO
AccessioniPrimary (citable) accession number: P97265
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: May 1, 1997
Last modified: October 1, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3