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Protein

Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A

Gene

MGAT5

Organism
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A (EC:2.4.1.155)
Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
GlcNAc-T V
Short name:
GNT-V
Mannoside acetylglucosaminyltransferase 5
N-acetylglucosaminyl-transferase V
Gene namesi
Name:MGAT5
OrganismiCricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Taxonomic identifieri10029 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaCricetidaeCricetinaeCricetulus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1313CytoplasmicSequence analysisAdd
BLAST
Transmembranei14 – 3017Helical; Signal-anchor for type II membrane proteinSequence analysisAdd
BLAST
Topological domaini31 – 740710LumenalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 740740Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase APRO_0000080521Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi109 – 1091N-linked (GlcNAc...)Sequence analysis
Glycosylationi114 – 1141N-linked (GlcNAc...)Sequence analysis
Glycosylationi117 – 1171N-linked (GlcNAc...)Sequence analysis
Glycosylationi333 – 3331N-linked (GlcNAc...)Sequence analysis
Glycosylationi432 – 4321N-linked (GlcNAc...)Sequence analysis
Glycosylationi446 – 4461N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 18 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG052469.
KOiK00744.

Family and domain databases

InterProiIPR027833. DUF4525.
IPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 1 hit.
PfamiPF15027. DUF4525. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P97259-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAFFTPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI
60 70 80 90 100
LDLSKRYIKA LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL
110 120 130 140 150
ESKVDNLVNG TGANSTNSTT AVPSLVSLEK ISVADIINGV QEKCVLPPMD
160 170 180 190 200
GYPHCEGKIK WMKDMWRSDP CYADYGVDGT SCSFFIYLSE VENWCPRLPW
210 220 230 240 250
RAKNPYEEAD HNSLAEIRTD FNILYSMMKK HEEFRWMRLR IRRMADAWIQ
260 270 280 290 300
AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW
310 320 330 340 350
SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI
360 370 380 390 400
DIVGLAQFKK TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW
410 420 430 440 450
GKWNLNPQQF YTMFPHTPDN SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV
460 470 480 490 500
YGKVDSFWKN KKIYLDIIHT YMEVHATVYG SSTKNIPSYV KNHGILSGRD
510 520 530 540 550
LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFSP PKSSKNTDFF
560 570 580 590 600
IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP
610 620 630 640 650
YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ
660 670 680 690 700
VCQENQLICE PSFFQHLNKE KDLLKYRVTC QSSELYKDIL VPSFYPKSKH
710 720 730 740
CVLQGDLLLF SCAGAHPTHQ RICPCRDFIK GQVALCKDCL
Length:740
Mass (Da):84,561
Last modified:May 1, 1997 - v1
Checksum:iDFAFCFBA7C172AE2
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti135 – 15521DIING…GYPHC → VQHPNSIGWCCPHLEWVFPV S in LEC4; loss of activity.
Add
BLAST
Natural varianti156 – 740585Missing in LEC4.
Add
BLAST
Natural varianti188 – 1881L → R in LEC4A; loss of activity.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62587 mRNA. Translation: AAC52925.1.
U62588 mRNA. Translation: AAC52926.1.
RefSeqiXP_007613026.1. XM_007614836.1.

Genome annotation databases

GeneIDi100760162.
KEGGicge:100760162.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U62587 mRNA. Translation: AAC52925.1.
U62588 mRNA. Translation: AAC52926.1.
RefSeqiXP_007613026.1. XM_007614836.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGT18. Glycosyltransferase Family 18.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100760162.
KEGGicge:100760162.

Organism-specific databases

CTDi4249.

Phylogenomic databases

HOVERGENiHBG052469.
KOiK00744.

Enzyme and pathway databases

UniPathwayiUPA00378.

Family and domain databases

InterProiIPR027833. DUF4525.
IPR026116. GlyclTrfase_18.
[Graphical view]
PANTHERiPTHR15075. PTHR15075. 1 hit.
PfamiPF15027. DUF4525. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A point mutation causes mistargeting of Golgi GlcNAc-TV in the Lec4A Chinese hamster ovary glycosylation mutant."
    Weinstein J., Sundaram S., Wang X., Delgado D., Basu R., Stanley P.
    J. Biol. Chem. 271:27462-27469(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEC4 AND LEC4A.

Entry informationi

Entry nameiMGT5A_CRIGR
AccessioniPrimary (citable) accession number: P97259
Secondary accession number(s): P70100, P70101
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: May 1, 1997
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.