P97259 (MGT5A_CRIGR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A
EC=2.4.1.155

Alternative name(s):
Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase
GlcNAc-T V
Short name=GNT-V
Mannoside acetylglucosaminyltransferase 5
N-acetylglucosaminyl-transferase V

Gene names

Name:

MGAT5

Organism

Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)

Taxonomic identifier

10029 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus

Protein attributes

Sequence length	740 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
Catalytic activity	UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus membrane; Single-pass type II membrane protein.
Sequence similarities	Belongs to the glycosyltransferase 18 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 740	740	Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A		PRO_0000080521
Regions
	Topological domain	1 – 13	13	Cytoplasmic Potential
	Transmembrane	14 – 30	17	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	31 – 740	710	Lumenal Potential
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...) Potential
	Glycosylation	114	1	N-linked (GlcNAc...) Potential
	Glycosylation	117	1	N-linked (GlcNAc...) Potential
	Glycosylation	333	1	N-linked (GlcNAc...) Potential
	Glycosylation	432	1	N-linked (GlcNAc...) Potential
	Glycosylation	446	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	135 – 155	21	DIING…GYPHC → VQHPNSIGWCCPHLEWVFPV S in LEC4; loss of activity.
	Natural variant	156 – 740	585	Missing in LEC4.
	Natural variant	188	1	L → R in LEC4A; loss of activity.

Sequences

	Sequence		Length	Mass (Da)
	P97259 [UniParc]. Last modified May 1, 1997. Version 1. Checksum: DFAFCFBA7C172AE2	FASTA	740	84,561
	10 20 30 40 50 60 MAFFTPWKLS SQKLGFFLVT FGFIWGMMLL HFTIQQRTQP ESSSMLREQI LDLSKRYIKA 70 80 90 100 110 120 LAEENRNVVD GPYAGVMTAY DLKKTLAVLL DNILQRIGKL ESKVDNLVNG TGANSTNSTT 130 140 150 160 170 180 AVPSLVSLEK ISVADIINGV QEKCVLPPMD GYPHCEGKIK WMKDMWRSDP CYADYGVDGT 190 200 210 220 230 240 SCSFFIYLSE VENWCPRLPW RAKNPYEEAD HNSLAEIRTD FNILYSMMKK HEEFRWMRLR 250 260 270 280 290 300 IRRMADAWIQ AIKSLAEKQN LEKRKRKKIL VHLGLLTKES GFKIAETAFS GGPLGELVQW 310 320 330 340 350 360 SDLITSLYLL GHDIRISASL AELKEIMKKV VGNRSGCPTV GDRIVELIYI DIVGLAQFKK 370 380 390 400 410 420 TLGPSWVHYQ CMLRVLDSFG TEPEFNHASY AQSKGHKTPW GKWNLNPQQF YTMFPHTPDN 430 440 450 460 470 480 SFLGFVVEQH LNSSDIHHIN EIKRQNQSLV YGKVDSFWKN KKIYLDIIHT YMEVHATVYG 490 500 510 520 530 540 SSTKNIPSYV KNHGILSGRD LQFLLRETKL FVGLGFPYEG PAPLEAIANG CAFLNPKFSP 550 560 570 580 590 600 PKSSKNTDFF IGKPTLRELT SQHPYAEVFI GRPHVWTVDL NNREEVEDAV KAILNQKIEP 610 620 630 640 650 660 YMPYEFTCEG MLQRINAFIE KQDFCHGQVM WPPLSALQVK LAEPGQSCKQ VCQENQLICE 670 680 690 700 710 720 PSFFQHLNKE KDLLKYRVTC QSSELYKDIL VPSFYPKSKH CVLQGDLLLF SCAGAHPTHQ 730 740 RICPCRDFIK GQVALCKDCL

References

[1]

"A point mutation causes mistargeting of Golgi GlcNAc-TV in the Lec4A Chinese hamster ovary glycosylation mutant." Weinstein J., Sundaram S., Wang X., Delgado D., Basu R., Stanley P. J. Biol. Chem. 271:27462-27469(1996) [PubMed: 8910328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEC4 AND LEC4A.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U62587 mRNA. Translation: AAC52925.1. U62588 mRNA. Translation: AAC52926.1.
RefSeq	XP_003497664.1. XM_003497616.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT18. Glycosyltransferase Family 18.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100760162.
Phylogenomic databases
HOVERGEN	HBG052469.
Family and domain databases
InterPro	IPR024144. Glyco_trans_18. [Graphical view]
PANTHER	PTHR15075. PTHR15075. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MGT5A_CRIGR

Accession

Primary (citable) accession number: P97259
Secondary accession number(s): P70100, P70101

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 28, 2003
Last sequence update:	May 1, 1997
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program