P97259 (MGT5A_CRIGR) Reviewed, UniProtKB/Swiss-Prot
Last modified
January 25, 2012.
Version 63.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A EC=2.4.1.155 Alternative name(s): Alpha-mannoside beta-1,6-N-acetylglucosaminyltransferase GlcNAc-T V Short name=GNT-V Mannoside acetylglucosaminyltransferase 5 N-acetylglucosaminyl-transferase V | ||
| Gene names |
| ||
| Organism | Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus) | ||
| Taxonomic identifier | 10029 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Cricetidae › Cricetinae › Cricetulus |
Protein attributes
| Sequence length | 740 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at transcript level |
General annotation (Comments)
| Function | Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. |
| Catalytic activity | UDP-N-acetyl-D-glucosamine + 6-(2-(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R = UDP + 6-(2,6-bis(N-acetyl-beta-D-glucosaminyl)-alpha-D-mannosyl)-beta-D-mannosyl-R. |
| Pathway | |
| Subcellular location | Golgi apparatus membrane; Single-pass type II membrane protein. |
| Sequence similarities | Belongs to the glycosyltransferase 18 family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Golgi apparatus Membrane |
| Domain | Signal-anchor Transmembrane Transmembrane helix |
| Molecular function | Glycosyltransferase Transferase |
| PTM | Glycoprotein |
| Gene Ontology (GO) | |
| Cellular component | Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase activity Inferred from electronic annotation. Source: EC |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 740 | 740 | Alpha-1,6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A | PRO_0000080521 | |||||
Regions | |||||||||
| Topological domain | 1 – 13 | 13 | Cytoplasmic Potential | ||||||
| Transmembrane | 14 – 30 | 17 | Helical; Signal-anchor for type II membrane protein; Potential | ||||||
| Topological domain | 31 – 740 | 710 | Lumenal Potential | ||||||
Amino acid modifications | |||||||||
| Glycosylation | 109 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 114 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 117 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 333 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 432 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 446 | 1 | N-linked (GlcNAc...) Potential | ||||||
Natural variations | |||||||||
| Natural variant | 135 – 155 | 21 | DIING…GYPHC → VQHPNSIGWCCPHLEWVFPV S in LEC4; loss of activity. | ||||||
| Natural variant | 156 – 740 | 585 | Missing in LEC4. | ||||||
| Natural variant | 188 | 1 | L → R in LEC4A; loss of activity. | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "A point mutation causes mistargeting of Golgi GlcNAc-TV in the Lec4A Chinese hamster ovary glycosylation mutant." Weinstein J., Sundaram S., Wang X., Delgado D., Basu R., Stanley P. J. Biol. Chem. 271:27462-27469(1996) [PubMed: 8910328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANTS LEC4 AND LEC4A. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U62587 mRNA. Translation: AAC52925.1. U62588 mRNA. Translation: AAC52926.1. |
| RefSeq | XP_003497664.1. XM_003497616.1. |
3D structure databases | |
| ModBase | Search... |
Protein family/group databases | |
| CAZy | GT18. Glycosyltransferase Family 18. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| GeneID | 100760162. |
Phylogenomic databases | |
| HOVERGEN | HBG052469. |
Family and domain databases | |
| InterPro | IPR024144. Glyco_trans_18. [Graphical view] |
| PANTHER | PTHR15075. PTHR15075. 1 hit. |
| ProtoNet | Search... |
Entry information
| Entry name | MGT5A_CRIGR | ||||||||
| Accession | Primary (citable) accession number: P97259 Secondary accession number(s): P70100, P70101 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with