P97046 (LMRA_LACLM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multidrug resistance ABC transporter ATP-binding and permease protein
EC=3.6.3.44

Gene names

Name:	lmrA
Ordered Locus Names:	llmg_1856

Organism

Lactococcus lactis subsp. cremoris (strain MG1363) [Complete proteome] [HAMAP]

Taxonomic identifier

416870 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Lactococcus

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Efflux transporter for a variety of amphiphilic cationic compounds, including antibiotics.
Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the ABC transporter superfamily. Multidrug exporter LmrA (TC 3.A.1.117.1) family. [View classification] Contains 1 ABC transmembrane type-1 domain. Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Antibiotic resistance Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Multidrug resistance ABC transporter ATP-binding and permease protein		PRO_0000092414
Regions
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	79 – 99	21	Helical; Potential
	Transmembrane	150 – 170	21	Helical; Potential
	Transmembrane	176 – 196	21	Helical; Potential
	Transmembrane	261 – 281	21	Helical; Potential
	Transmembrane	292 – 312	21	Helical; Potential
	Domain	38 – 317	280	ABC transmembrane type-1
	Domain	349 – 584	236	ABC transporter
	Nucleotide binding	382 – 389	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	P97046 [UniParc]. Last modified July 25, 2003. Version 3. Checksum: CCE4E7D4EF912B8D	FASTA	590	64,617
	10 20 30 40 50 60 MERGPQMANR IEGKAVDKTS IKHFVKLIRA AKPRYLFFVI GIVAGIIGTL IQLQVPKMVQ 70 80 90 100 110 120 PLINSFGHGV NGGKVALVIA LYIGSAAVSA IAAIVLGIFG ESVVKNLRTR VWDKMIHLPV 130 140 150 160 170 180 KYFDEVKTGE MSSRLANDTT QVKNLIANSI PQAFTSILLL VGSIIFMLQM QWRLTLAMII 190 200 210 220 230 240 AVPIVMLIMF PIMTFGQKIG WTRQDSLANF QGIASESLSE IRLVKSSNAE KQASKKAEND 250 260 270 280 290 300 VNALYKIGVK EAVFDGLMSP VMMLSMMLMI FGLLAYGIYL ISTGVMSLGT LLGMMMYLMN 310 320 330 340 350 360 LIGVVPTVAT FFTELAKASG STGRLTELLD EEQEVLHQGD SLDLEGKTLS AHHVDFAYDD 370 380 390 400 410 420 SEQILHDISF EAQPNSIIAF AGPSGGGKST IFSLLERFYQ PTAGEITIGG QPIDSVSLEN 430 440 450 460 470 480 WRSQIGFVSQ DSAIMAGTIR ENLTYGLEGN FTDEDLWQVL DLAFARSFVE NMPDQLNTEV 490 500 510 520 530 540 GERGVKISGG QRQRLAIARA FLRNPKILML DEATASLDSE SESMVQRALD SLMKGRTTLV 550 560 570 580 590 IAHRLSTIVD ADKIYFIEKG EITGSGKHNE LVATHPLYAK YVSEQLTVGQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multidrug resistance mediated by a bacterial homolog of the human multidrug transporter MDR1." van Veen H.W., Venema K., Bolhuis H., Oussenko I., Kok J., Poolman B., Driessen A.J., Konings W.N. Proc. Natl. Acad. Sci. U.S.A. 93:10668-10672(1996) [PubMed: 8855237] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the lactic acid bacterial paradigm Lactococcus lactis subsp. cremoris MG1363." Wegmann U., O'Connell-Motherway M., Zomer A., Buist G., Shearman C., Canchaya C., Ventura M., Goesmann A., Gasson M.J., Kuipers O.P., van Sinderen D., Kok J. J. Bacteriol. 189:3256-3270(2007) [PubMed: 17307855] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MG1363.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63741 Genomic DNA. Translation: AAB49750.1. AM406671 Genomic DNA. Translation: CAL98427.1.
RefSeq	YP_001033128.1. NC_009004.1.
3D structure databases
ProteinModelPortal	P97046.
SMR	P97046. Positions 349-589.
ModBase	Search...
Protein-protein interaction databases
STRING	P97046.
Protein family/group databases
TCDB	3.A.1.117.1. ATP-binding cassette (ABC) superfamily.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4797383.
GenomeReviews	Gene locus llmg_1856 in contig AM406671_GR.
KEGG	llm:llmg_1856.
PATRIC	22284896. VBILacLac4574_1901.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1132.
HOGENOM	HBG758042.
OMA	NELVATH.
ProtClustDB	CLSK697366.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K06147.
Pfam	PF00664. ABC_membrane. 1 hit. PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF90123. ABC_TM_1. 1 hit.
PROSITE	PS50929. ABC_TM1F. 1 hit. PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LMRA_LACLM

Accession

Primary (citable) accession number: P97046
Secondary accession number(s): A2RMA0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
Last sequence update:	July 25, 2003
Last modified:	January 25, 2012
This is version 88 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program